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POLA2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  POLA2
Gene Name:  polymerase (DNA directed), alpha 2, accessory subunit
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH182419.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00040977

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1797nts         Open reading frame : 1 to 1797
Reference Sequence Alignment

ATGTCCGCATCCGCCCAGCAGCTGGCGGAGGAGCTGCAGATCTTCGGCCTAGACTGCGAG
GAGGCTCTAATTGAGAAATTGGTAGAGCTTTGTGTTCAGTATGGACAGAATGAGGAGGGA
ATGGTAGGCGAGCTTATAGCCTTCTGCACCAGCACACATAAAGTTGGCCTTACCTCAGAG
ATCCTGAACTCTTTTGAGCATGAGTTTCTGAGCAAAAGATTATCGAAAGCCAGGCATAGT
ACCTGCAAGGACAGTGGCCATGCAGGAGCTAGAGACATTGTTTCCATTCAAGAGCTAATT
GAAGTGGAAGAAGAAGAGGAAATCCTCTTGAACTCTTACACCACACCTTCAAAGGGTTCT
CAGAAGCGAGCTATCTCTACCCCAGAAACCCCCCTAACAAAAAGGAGTGTGTCAACTCGT
AGCCCCCATCAGCTACTCTCACCGTCAAGTTTCTCTCCAAGTGCTACTCCCTCCCAGAAA
TACAACTCACGAAGTAACCGAGGAGAAGTGGTTACCTCCTTCGGCTTAGCACAGGGAGTA
TCTTGGTCTGGGAGAGGAGGAGCTGGAAACATCAGCCTGAAGGTCTTGGGATGTCCAGAG
GCACTAACTGGGAGCTACAAATCCATGTTTCAGAAGCTCCCAGACATTCGAGAAGTTCTG
ACCTGTAAGATAGAAGAACTTGGCAGCGAACTCAAGGAACATTACAAGATTGAAGCTTTC
ACTCCTTTGCTAGCCCCAGCACAGGAGCCTGTCACTCTGCTGGGCCAGATTGGCTGTGAT
AGCAACGGGAAGCTGAACAACAAGTCAGTGATTCTCGAGGGAGACCGGGAACATTCCTCG
GGTGCTCAAATTCCAGTGGATTTATCTGAGCTTAAGGAATATTCTCTGTTTCCTGGACAG
GTTGTAATTATGGAAGGAATCAACACCACTGGTAGGAAACTTGTTGCCACCAAACTCTAC
GAGGGTGTGCCACTTCCATTTTATCAGCCCACTGAAGAGGATGCAGACTTTGAGCAAAGC
ATGGTCCTGGTTGCCTGTGGACCATACACCACATCTGACAGCATCACGTATGACCCCCTG
CTTGACCTGATTGCTGTCATCAACCATGACCGGCCAGATGTCTGCATCCTGTTTGGCCCT
TTCCTGGATGCTAAGCATGAACAGGTGGAGAATTGTCTACTGACAAGTCCATTTGAAGAC
ATTTTCAAGCAGTGTCTACGAACAATTATTGAAGGCACAAGAAGCTCCGGCTCCCACCTT
GTCTTTGTCCCGTCATTGAGAGATGTGCACCATGAGCCTGTGTACCCCCAGCCGCCTTTC
AGCTACTCCGATCTGTCTCGAGAGGACAAAAAGCAAGTACAGTTTGTGTCCGAGCCCTGC
AGCCTCTCCATAAACGGAGTGATCTTCGGCTTGACATCCACAGATCTGCTTTTCCACCTG
GGGGCCGAGGAGATCAGTAGTTCTTCCGGAACTTCAGACAGATTCAGCCGAATACTCAAG
CACATCTTGACCCAGAGGAGCTACTACCCACTCTACCCGCCCCAAGAAGACATGGCCATT
GACTATGAGTCGTTCTATGTTTACGCACAGCTGCCTGTCACCCCAGATGTCCTCATCATC
CCGTCAGAGCTGAGGTACTTCGTGAAGGATGTCCTCGGCTGTGTCTGTGTGAACCCTGGG
CGCCTTACCAAAGGGCAGGTGGGAGGCACCTTCGCCCGACTCTACCTTAGGAGGCCGGCA
GCGGACGGGGCAGAGAGGCAGAGCCCATGCATTGCTGTGCAGGTCGTCAGGATCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2148
Start on reference sequence 352
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : POLA2
Symbol Nomenclature : POLA2
Designation : DNA polymerase alpha 70 kDa subunit|DNA polymerase alpha subunit B|polymerase (DNA directed), alpha 2 (70kD subunit)|polymerase (DNA-directed), alpha (70kD)
Full Nomenclature : polymerase (DNA directed), alpha 2, accessory subunit
GENEID : 23649
HGNC : 30073
Target GenBank: BC001347

 

Reference Sequence Alignment

  

HsCD00040977      ATGTCCGCATCCGCCCAGCAGCTGGCGGAGGAGCTGCAGATCTTCGGCCTAGACTGCGAG
NM_002689.2       ATGTCCGCATCCGCCCAGCAGCTGGCGGAGGAGCTGCAGATCTTCGGCCTAGACTGCGAG
                  ************************************************************

HsCD00040977      GAGGCTCTAATTGAGAAATTGGTAGAGCTTTGTGTTCAGTATGGACAGAATGAGGAGGGA
NM_002689.2       GAGGCTCTAATTGAGAAATTGGTAGAGCTTTGTGTTCAGTATGGACAGAATGAGGAGGGA
                  ************************************************************

HsCD00040977      ATGGTAGGCGAGCTTATAGCCTTCTGCACCAGCACACATAAAGTTGGCCTTACCTCAGAG
NM_002689.2       ATGGTAGGCGAGCTTATAGCCTTCTGCACCAGCACACATAAAGTTGGCCTTACCTCAGAG
                  ************************************************************

HsCD00040977      ATCCTGAACTCTTTTGAGCATGAGTTTCTGAGCAAAAGATTATCGAAAGCCAGGCATAGT
NM_002689.2       ATCCTGAACTCTTTTGAGCATGAGTTTCTGAGCAAAAGATTATCGAAAGCCAGGCATAGT
                  ************************************************************

HsCD00040977      ACCTGCAAGGACAGTGGCCATGCAGGAGCTAGAGACATTGTTTCCATTCAAGAGCTAATT
NM_002689.2       ACCTGCAAGGACAGTGGCCATGCAGGAGCTAGAGACATTGTTTCCATTCAAGAGCTAATT
                  ************************************************************

HsCD00040977      GAAGTGGAAGAAGAAGAGGAAATCCTCTTGAACTCTTACACCACACCTTCAAAGGGTTCT
NM_002689.2       GAAGTGGAAGAAGAAGAGGAAATCCTCTTGAACTCTTACACCACACCTTCAAAGGGTTCT
                  ************************************************************

HsCD00040977      CAGAAGCGAGCTATCTCTACCCCAGAAACCCCCCTAACAAAAAGGAGTGTGTCAACTCGT
NM_002689.2       CAGAAGCGAGCTATCTCTACCCCAGAAACCCCCCTAACAAAAAGGAGTGTGTCAACTCGT
                  ************************************************************

HsCD00040977      AGCCCCCATCAGCTACTCTCACCGTCAAGTTTCTCTCCAAGTGCTACTCCCTCCCAGAAA
NM_002689.2       AGCCCCCATCAGCTACTCTCACCGTCAAGTTTCTCTCCAAGTGCTACTCCCTCCCAGAAA
                  ************************************************************

HsCD00040977      TACAACTCACGAAGTAACCGAGGAGAAGTGGTTACCTCCTTCGGCTTAGCACAGGGAGTA
NM_002689.2       TACAACTCACGAAGTAACCGAGGAGAAGTGGTTACCTCCTTCGGCTTAGCACAGGGAGTA
                  ************************************************************

HsCD00040977      TCTTGGTCTGGGAGAGGAGGAGCTGGAAACATCAGCCTGAAGGTCTTGGGATGTCCAGAG
NM_002689.2       TCTTGGTCTGGGAGAGGAGGAGCTGGAAACATCAGCCTGAAGGTCTTGGGATGTCCAGAG
                  ************************************************************

HsCD00040977      GCACTAACTGGGAGCTACAAATCCATGTTTCAGAAGCTCCCAGACATTCGAGAAGTTCTG
NM_002689.2       GCACTAACTGGGAGCTACAAATCCATGTTTCAGAAGCTCCCAGACATTCGAGAAGTTCTG
                  ************************************************************

HsCD00040977      ACCTGTAAGATAGAAGAACTTGGCAGCGAACTCAAGGAACATTACAAGATTGAAGCTTTC
NM_002689.2       ACCTGTAAGATAGAAGAACTTGGCAGCGAACTCAAGGAACATTACAAGATTGAAGCTTTC
                  ************************************************************

HsCD00040977      ACTCCTTTGCTAGCCCCAGCACAGGAGCCTGTCACTCTGCTGGGCCAGATTGGCTGTGAT
NM_002689.2       ACTCCTTTGCTAGCCCCAGCACAGGAGCCTGTCACTCTGCTGGGCCAGATTGGCTGTGAT
                  ************************************************************

HsCD00040977      AGCAACGGGAAGCTGAACAACAAGTCAGTGATTCTCGAGGGAGACCGGGAACATTCCTCG
NM_002689.2       AGCAACGGGAAGCTGAACAACAAGTCAGTGATTCTCGAGGGAGACCGGGAACATTCCTCG
                  ************************************************************

HsCD00040977      GGTGCTCAAATTCCAGTGGATTTATCTGAGCTTAAGGAATATTCTCTGTTTCCTGGACAG
NM_002689.2       GGTGCTCAAATTCCAGTGGATTTATCTGAGCTTAAGGAATATTCTCTGTTTCCTGGACAG
                  ************************************************************

HsCD00040977      GTTGTAATTATGGAAGGAATCAACACCACTGGTAGGAAACTTGTTGCCACCAAACTCTAC
NM_002689.2       GTTGTAATTATGGAAGGAATCAACACCACTGGTAGGAAACTTGTTGCCACCAAACTCTAC
                  ************************************************************

HsCD00040977      GAGGGTGTGCCACTTCCATTTTATCAGCCCACTGAAGAGGATGCAGACTTTGAGCAAAGC
NM_002689.2       GAGGGTGTGCCACTTCCATTTTATCAGCCCACTGAAGAGGATGCAGACTTTGAGCAAAGC
                  ************************************************************

HsCD00040977      ATGGTCCTGGTTGCCTGTGGACCATACACCACATCTGACAGCATCACGTATGACCCCCTG
NM_002689.2       ATGGTCCTGGTTGCCTGTGGACCATACACCACATCTGACAGCATCACGTATGACCCCCTG
                  ************************************************************

HsCD00040977      CTTGACCTGATTGCTGTCATCAACCATGACCGGCCAGATGTCTGCATCCTGTTTGGCCCT
NM_002689.2       CTTGACCTGATTGCTGTCATCAACCATGACCGGCCAGATGTCTGCATCCTGTTTGGCCCT
                  ************************************************************

HsCD00040977      TTCCTGGATGCTAAGCATGAACAGGTGGAGAATTGTCTACTGACAAGTCCATTTGAAGAC
NM_002689.2       TTCCTGGATGCTAAGCATGAACAGGTGGAGAATTGTCTACTGACAAGTCCATTTGAAGAC
                  ************************************************************

HsCD00040977      ATTTTCAAGCAGTGTCTACGAACAATTATTGAAGGCACAAGAAGCTCCGGCTCCCACCTT
NM_002689.2       ATTTTCAAGCAGTGTCTACGAACAATTATTGAAGGCACAAGAAGCTCCGGCTCCCACCTT
                  ************************************************************

HsCD00040977      GTCTTTGTCCCGTCATTGAGAGATGTGCACCATGAGCCTGTGTACCCCCAGCCGCCTTTC
NM_002689.2       GTCTTTGTCCCGTCATTGAGAGATGTGCACCATGAGCCTGTGTACCCCCAGCCGCCTTTC
                  ************************************************************

HsCD00040977      AGCTACTCCGATCTGTCTCGAGAGGACAAAAAGCAAGTACAGTTTGTGTCCGAGCCCTGC
NM_002689.2       AGCTACTCCGATCTGTCTCGAGAGGACAAAAAGCAAGTACAGTTTGTGTCCGAGCCCTGC
                  ************************************************************

HsCD00040977      AGCCTCTCCATAAACGGAGTGATCTTCGGCTTGACATCCACAGATCTGCTTTTCCACCTG
NM_002689.2       AGCCTCTCCATAAACGGAGTGATCTTCGGCTTGACATCCACAGATCTGCTTTTCCACCTG
                  ************************************************************

HsCD00040977      GGGGCCGAGGAGATCAGTAGTTCTTCCGGAACTTCAGACAGATTCAGCCGAATACTCAAG
NM_002689.2       GGGGCCGAGGAGATCAGTAGTTCTTCCGGAACTTCAGACAGATTCAGCCGAATACTCAAG
                  ************************************************************

HsCD00040977      CACATCTTGACCCAGAGGAGCTACTACCCACTCTACCCGCCCCAAGAAGACATGGCCATT
NM_002689.2       CACATCTTGACCCAGAGGAGCTACTACCCACTCTACCCGCCCCAAGAAGACATGGCCATT
                  ************************************************************

HsCD00040977      GACTATGAGTCGTTCTATGTTTACGCACAGCTGCCTGTCACCCCAGATGTCCTCATCATC
NM_002689.2       GACTATGAGTCGTTCTATGTTTACGCACAGCTGCCTGTCACCCCAGATGTCCTCATCATC
                  ************************************************************

HsCD00040977      CCGTCAGAGCTGAGGTACTTCGTGAAGGATGTCCTCGGCTGTGTCTGTGTGAACCCTGGG
NM_002689.2       CCGTCAGAGCTGAGGTACTTCGTGAAGGATGTCCTCGGCTGTGTCTGTGTGAACCCTGGG
                  ************************************************************

HsCD00040977      CGCCTTACCAAAGGGCAGGTGGGAGGCACCTTCGCCCGACTCTACCTTAGGAGGCCGGCA
NM_002689.2       CGCCTTACCAAAGGGCAGGTGGGAGGCACCTTCGCCCGACTCTACCTTAGGAGGCCGGCA
                  ************************************************************

HsCD00040977      GCGGACGGGGCAGAGAGGCAGAGCCCATGCATTGCTGTGCAGGTCGTCAGGATCTTG
NM_002689.2       GCGGACGGGGCAGAGAGGCAGAGCCCATGCATTGCTGTGCAGGTCGTCAGGATCTGA
                  ******************************************************* .

Pathways

Reactome : Activation of the pre-replicative complex
Reactome : Cell Cycle
Reactome : Cell Cycle, Mitotic
Reactome : Chromosome Maintenance
Reactome : DNA Replication
Reactome : DNA Replication Pre-Initiation
Reactome : DNA replication initiation
Reactome : DNA strand elongation
Reactome : E2F mediated regulation of DNA replication
Reactome : Extension of Telomeres
Reactome : G1/S Transition
Reactome : Inhibition of replication initiation of damaged DNA by RB1/E2F1
Reactome : Lagging Strand Synthesis
Reactome : Leading Strand Synthesis
Reactome : M/G1 Transition
Reactome : Mitotic G1-G1/S phases
Reactome : Mitotic M-M/G1 phases
Reactome : Polymerase switching
Reactome : Polymerase switching on the C-strand of the telomere
Reactome : Processive synthesis on the lagging strand
Reactome : Removal of the Flap Intermediate
Reactome : S Phase
Reactome : Synthesis of DNA
Reactome : Telomere C-strand (Lagging Strand) Synthesis
Reactome : Telomere C-strand synthesis initiation
Reactome : Telomere Maintenance
WikiPathway : DNA Replication
WikiPathway : G1 to S cell cycle control
WikiPathway : Lymphocyte TarBase
WikiPathway : Muscle cell TarBase

Protein

UniProt : Q14181
HPRD : 10160

Clone

Cloning Information : 182419
HIP Master Clone ID : 157363
Original Clone ID : FLH182419.01L

Organism


TAX_ID : 9606
Species Specific ID: 23649

Genome

Chromosome : 11
Map Location : 11q13.1

Reagents

Labome : POLA2-antibody