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PPP1CA (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  PPP1CA
Gene Name:  protein phosphatase 1, catalytic subunit, alpha isozyme
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH026428.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00004106

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 993nts         Open reading frame : 1 to 993
Reference Sequence Alignment

ATGTCCGACAGCGAGAAGCTCAACCTGGACTCGATCATCGGGCGCCTGCTGGAAGTGCAG
GGCTCGCGGCCTGGCAAGAATGTACAGCTGACAGAGAACGAGATCCGCGGTCTGTGCCTG
AAATCCCGGGAGATTTTTCTGAGCCAGCCCATTCTTCTGGAGCTGGAGGCACCCCTCAAG
ATCTGCGGTGACATACACGGCCAGTACTACGACCTTCTGCGACTATTTGAGTATGGCGGT
TTCCCTCCCGAGAGCAACTACCTCTTTCTGGGGGACTATGTGGACAGGGGCAAGCAGTCC
TTGGAGACCATCTGCCTGCTGCTGGCCTATAAGATCAAGTACCCCGAGAACTTCTTCCTG
CTCCGTGGGAACCACGAGTGTGCCAGCATCAACCGCATCTATGGTTTCTACGATGAGTGC
AAGAGACGCTACAACATCAAACTGTGGAAAACCTTCACTGACTGCTTCAACTGCCTGCCC
ATCGCGGCCATAGTGGACGAAAAGATCTTCTGCTGCCACGGAGGCCTGTCCCCGGACCTG
CAGTCTATGGAGCAGATTCGGCGGATCATGCGGCCCACAGATGTGCCTGACCAGGGCCTG
CTGTGTGACCTGCTGTGGTCTGACCCTGACAAGGACGTGCAGGGCTGGGGCGAGAACGAC
CGTGGCGTCTCTTTTACCTTTGGAGCCGAGGTGGTGGCCAAGTTCCTCCACAAGCACGAC
TTGGACCTCATCTGCCGAGCACACCAGGTGGTAGAAGACGGCTACGAGTTCTTTGCCAAG
CGGCAGCTGGTGACACTTTTCTCAGCTCCCAACTACTGTGGCGAGTTTGACAATGCTGGC
GCCATGATGAGTGTGGACGAGACCCTCATGTGCTCTTTCCAGATCCTCAAGCCCGCCGAC
AAGAACAAGGGGAAGTACGGGCAGTTCAGTGGCCTGAACCCTGGAGGCCGACCCATCACC
CCACCCCGCAATTCCGCCAAAGCCAAGAAATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1057
Start on reference sequence 65
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PPP1CA
Symbol Nomenclature : PPP1CA
SYNONYM : PP-1A; PP1A; PP1alpha; PPP1A
Designation : protein phosphatase 1, catalytic subunit, alpha isoform|serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit|serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Full Nomenclature : protein phosphatase 1, catalytic subunit, alpha isozyme
GENEID : 5499
GI : 60656412
GenBank Accession : AY889979
HGNC : 9281
MIM : 176875
Vega : OTTHUMG00000167671
Target GenBank: BC008010

 

Reference Sequence Alignment

  

HsCD00004106        ATGTCCGACAGCGAGAAGCTCAACCTGGACTCGATCATCGGGCGCCTGCTGGAAG-----
NM_002710.3         ATGGCGGATTTAGATAAACTCAACATCGACAGCATTATCCAACGGCTGCTGGAAG-----
NM_002708.3         ATGTCCGACAGCGAGAAGCTCAACCTGGACTCGATCATCGGGCGCCTGCTGGAAG-----
NM_001244974.1      ATGGCGGATTTAGATAAACTCAACATCGACAGCATTATCCAACGGCTGCTGGAAG-----
NM_001008709.1      ATGTCCGACAGCGAGAAGCTCAACCTGGACTCGATCATCGGGCGCCTGCTGGAAGGGTCC
                    *** * ** : .** **.******.* ***:  ** *** ..** **********     

HsCD00004106        ----------------------------TGCAGGGCTCGCGGCCTGGCAAGAATGTACAG
NM_002710.3         ----------------------------TGAGAGGGTCCAAGCCTGGTAAGAATGTCCAG
NM_002708.3         ----------------------------TGCAGGGCTCGCGGCCTGGCAAGAATGTACAG
NM_001244974.1      ----------------------------TGAGAGGGTCCAAGCCTGGTAAGAATGTCCAG
NM_001008709.1      AGGGTCCTGACACCCCATTGCGCCCCAGTGCAGGGCTCGCGGCCTGGCAAGAATGTACAG
                                                **...** ** ..****** ********.***

HsCD00004106        CTGACAGAGAACGAGATCCGCGGTCTGTGCCTGAAATCCCGGGAGATTTTTCTGAGCCAG
NM_002710.3         CTTCAGGAGAATGAAATCAGAGGACTGTGCTTAAAGTCTCGTGAAATCTTTCTCAGTCAG
NM_002708.3         CTGACAGAGAACGAGATCCGCGGTCTGTGCCTGAAATCCCGGGAGATTTTTCTGAGCCAG
NM_001244974.1      CTTCAGGAGAATGAAATCAGAGGACTGTGCTTAAAGTCTCGTGAAATCTTTCTCAGTCAG
NM_001008709.1      CTGACAGAGAACGAGATCCGCGGTCTGTGCCTGAAATCCCGGGAGATTTTTCTGAGCCAG
                    ** ...***** **.***.*.**:****** *.**.** ** **.** ***** ** ***

HsCD00004106        CCCATTCTTCTGGAGCTGGAGGCACCCCTCAAGATCTGCGGTGACATACACGGCCAGTAC
NM_002710.3         CCTATCCTACTAGAACTTGAAGCACCACTCAAAATATGTGGTGACATCCATGGACAATAC
NM_002708.3         CCCATTCTTCTGGAGCTGGAGGCACCCCTCAAGATCTGCGGTGACATACACGGCCAGTAC
NM_001244974.1      CCTATCCTACTAGAACTTGAAGCACCACTCAAAATATGTGGTGACATCCATGGACAATAC
NM_001008709.1      CCCATTCTTCTGGAGCTGGAGGCACCCCTCAAGATCTGCGGTGACATACACGGCCAGTAC
                    ** ** **:**.**.** **.*****.*****.**.** ********.** **.**.***

HsCD00004106        TACGACCTTCTGCGACTATTTGAGTATGGCGGTTTCCCTCCCGAGAGCAACTACCTCTTT
NM_002710.3         TATGATTTGCTGCGACTTTTTGAGTACGGTGGTTTCCCACCAGAAAGCAACTACCTGTTT
NM_002708.3         TACGACCTTCTGCGACTATTTGAGTATGGCGGTTTCCCTCCCGAGAGCAACTACCTCTTT
NM_001244974.1      TATGATTTGCTGCGACTTTTTGAGTACGGTGGTTTCCCACCAGAAAGCAACTACCTGTTT
NM_001008709.1      TACGACCTTCTGCGACTATTTGAGTATGGCGGTTTCCCTCCCGAGAGCAACTACCTCTTT
                    ** **  * ********:******** ** ********:**.**.*********** ***

HsCD00004106        CTGGGGGACTATGTGGACAGGGGCAAGCAGTCCTTGGAGACCATCTGCCTGCTGCTGGCC
NM_002710.3         CTTGGGGACTATGTGGACAGGGGAAAGCAGTCATTGGAGACGATCTGCCTCTTACTGGCC
NM_002708.3         CTGGGGGACTATGTGGACAGGGGCAAGCAGTCCTTGGAGACCATCTGCCTGCTGCTGGCC
NM_001244974.1      CTTGGGGACTATGTGGACAGGGGAAAGCAGTCATTGGAGACGATCTGCCTCTTACTGGCC
NM_001008709.1      CTGGGGGACTATGTGGACAGGGGCAAGCAGTCCTTGGAGACCATCTGCCTGCTGCTGGCC
                    ** ********************.********.******** ********  *.******

HsCD00004106        TATAAGATCAAGTACCCCGAGAACTTCTTCCTGCTCCGTGGGAACCACGAGTGTGCCAGC
NM_002710.3         TACAAAATAAAATATCCTGAGAATTTTTTTCTTCTCAGAGGGAACCATGAATGTGCCAGC
NM_002708.3         TATAAGATCAAGTACCCCGAGAACTTCTTCCTGCTCCGTGGGAACCACGAGTGTGCCAGC
NM_001244974.1      TACAAAATAAAATATCCTGAGAATTTTTTTCTTCTCAGAGGGAACCATGAATGTGCCAGC
NM_001008709.1      TATAAGATCAAGTACCCCGAGAACTTCTTCCTGCTCCGTGGGAACCACGAGTGTGCCAGC
                    ** **.**.**.** ** ***** ** ** ** ***.*:******** **.*********

HsCD00004106        ATCAACCGCATCTATGGTTTCTACGATGAGTGCAAGAGACGCTACAACATCAAACTGTGG
NM_002710.3         ATCAACAGAATTTATGGATTTTATGATGAATGTAAAAGAAGATACAACATTAAACTATGG
NM_002708.3         ATCAACCGCATCTATGGTTTCTACGATGAGTGCAAGAGACGCTACAACATCAAACTGTGG
NM_001244974.1      ATCAACAGAATTTATGGATTTTATGATGAATGTAAAAGAAGATACAACATTAAACTATGG
NM_001008709.1      ATCAACCGCATCTATGGTTTCTACGATGAGTGCAAGAGACGCTACAACATCAAACTGTGG
                    ******.*.** *****:** ** *****.** **.***.*.******** *****.***

HsCD00004106        AAAACCTTCACTGACTGCTTCAACTGCCTGCCCATCGCGGCCATAGTGGACGAAAAGATC
NM_002710.3         AAAACTTTCACAGACTGTTTTAACTGTTTACCGATAGCAGCCATCGTGGATGAGAAGATA
NM_002708.3         AAAACCTTCACTGACTGCTTCAACTGCCTGCCCATCGCGGCCATAGTGGACGAAAAGATC
NM_001244974.1      AAAACTTTCACAGACTGTTTTAACTGTTTACCGATAGCAGCCATCGTGGATGAGAAGATA
NM_001008709.1      AAAACCTTCACTGACTGCTTCAACTGCCTGCCCATCGCGGCCATAGTGGACGAAAAGATC
                    ***** *****:***** ** *****  *.** **.**.*****.***** **.*****.

HsCD00004106        TTCTGCTGCCACGGAGGCCTGTCCCCGGACCTGCAGTCTATGGAGCAGATTCGGCGGATC
NM_002710.3         TTCTGCTGTCATGGAGGTTTATCACCAGATCTTCAATCTATGGAGCAGATTCGGCGAATT
NM_002708.3         TTCTGCTGCCACGGAGGCCTGTCCCCGGACCTGCAGTCTATGGAGCAGATTCGGCGGATC
NM_001244974.1      TTCTGCTGTCATGGAGGTTTATCACCAGATCTTCAATCTATGGAGCAGATTCGGCGAATT
NM_001008709.1      TTCTGCTGCCACGGAGGCCTGTCCCCGGACCTGCAGTCTATGGAGCAGATTCGGCGGATC
                    ******** ** *****  *.**.**.** ** **.********************.** 

HsCD00004106        ATGCGGCCCACAGATGTGCCTGACCAGGGCCTGCTGTGTGACCTGCTGTGGTCTGACCCT
NM_002710.3         ATGCGACCAACTGATGTACCAGATCAAGGTCTTCTTTGTGATCTTTTGTGGTCTGACCCC
NM_002708.3         ATGCGGCCCACAGATGTGCCTGACCAGGGCCTGCTGTGTGACCTGCTGTGGTCTGACCCT
NM_001244974.1      ATGCGACCAACTGATGTACCAGATCAAGGTCTTCTTTGTGATCTTTTGTGGTCTGACCCC
NM_001008709.1      ATGCGGCCCACAGATGTGCCTGACCAGGGCCTGCTGTGTGACCTGCTGTGGTCTGACCCT
                    *****.**.**:*****.**:** **.** ** ** ***** **  ************* 

HsCD00004106        GACAAGGACGTGCAGGGCTGGGGCGAGAACGACCGTGGCGTCTCTTTTACCTTTGGAGCC
NM_002710.3         GATAAAGATGTCTTAGGCTGGGGTGAAAATGACAGAGGAGTGTCCTTCACATTTGGTGCA
NM_002708.3         GACAAGGACGTGCAGGGCTGGGGCGAGAACGACCGTGGCGTCTCTTTTACCTTTGGAGCC
NM_001244974.1      GATAAAGATGTCTTAGGCTGGGGTGAAAATGACAGAGGAGTGTCCTTCACATTTGGTGCA
NM_001008709.1      GACAAGGACGTGCAGGGCTGGGGCGAGAACGACCGTGGCGTCTCTTTTACCTTTGGAGCC
                    ** **.** **  :.******** **.** ***.*:**.** ** ** **.*****:**.

HsCD00004106        GAGGTGGTGGCCAAGTTCCTCCACAAGCACGACTTGGACCTCATCTGCCGAGCACACCAG
NM_002710.3         GAAGTGGTTGCAAAATTTCTCCATAAGCATGATTTGGATCTTATATGTAGAGCCCATCAG
NM_002708.3         GAGGTGGTGGCCAAGTTCCTCCACAAGCACGACTTGGACCTCATCTGCCGAGCACACCAG
NM_001244974.1      GAAGTGGTTGCAAAATTTCTCCATAAGCATGATTTGGATCTTATATGTAGAGCCCATCAG
NM_001008709.1      GAGGTGGTGGCCAAGTTCCTCCACAAGCACGACTTGGACCTCATCTGCCGAGCACACCAG
                    **.***** **.**.** ***** ***** ** ***** ** **.** .****.** ***

HsCD00004106        GTGGTAGAAGACGGCTACGAGTTCTTTGCCAAGCGGCAGCTGGTGACACTTTTCTCAGCT
NM_002710.3         GTGGTTGAAGATGGATATGAATTTTTTGCAAAGAGGCAGTTGGTCACTCTGTTTTCTGCG
NM_002708.3         GTGGTAGAAGACGGCTACGAGTTCTTTGCCAAGCGGCAGCTGGTGACACTTTTCTCAGCT
NM_001244974.1      GTGGTTGAAGATGGATATGAATTTTTTGCAAAGAGGCAGTTGGTCACTCTGTTTTCTGCG
NM_001008709.1      GTGGTAGAAGACGGCTACGAGTTCTTTGCCAAGCGGCAGCTGGTGACACTTTTCTCAGCT
                    *****:***** **.** **.** *****.***.***** **** **:** ** **:** 

HsCD00004106        CCCAACTACTGTGGCGAGTTTGACAATGCTGGCGCCATGATGAGTGTGGACGAGACCCTC
NM_002710.3         CCCAATTATTGCGGAGAGTTTGACAATGCAGGTGCCATGATGAGTGTGGATGAAACACTA
NM_002708.3         CCCAACTACTGTGGCGAGTTTGACAATGCTGGCGCCATGATGAGTGTGGACGAGACCCTC
NM_001244974.1      CCCAATTATTGCGGAGAGTTTGACAATGCAGGTGCCATGATGAGTGTGGATGAAACACTA
NM_001008709.1      CCCAACTACTGTGGCGAGTTTGACAATGCTGGCGCCATGATGAGTGTGGACGAGACCCTC
                    ***** ** ** **.**************:** ***************** **.**.**.

HsCD00004106        ATGTGCTCTTTCCAGATCCTCAAGCCCGCCGACAAGAACAAGGGGAAGTACGGGCAGTTC
NM_002710.3         ATGTGTTCTTTTCAGATTTTAAAGCCTGCAGAGAAAAAGAAGCCAAATGCCACGAGACCT
NM_002708.3         ATGTGCTCTTTCCAGATCCTCAAGCCCGCCGACAAGAACAAGGGGAAGTACGGGCAGTTC
NM_001244974.1      ATGTGTTCTTTTCAGATTTTAAAGCCTGCAGAGAAAAAGAAGCCAAATGCCACGAGACCT
NM_001008709.1      ATGTGCTCTTTCCAGATCCTCAAGCCCGCCGACAAGAACAAGGGGAAGTACGGGCAGTTC
                    ***** ***** *****  *.***** **.** **.** ***  .**  .*. *...   

HsCD00004106        AGTGGCCTGAACCCTGGAGGCCGACCCATCACCCCACCCCGCAATTCCGCCAAAGCCAAG
NM_002710.3         GTAACGCCTCCAAGGGGTATGATCACAAAGCAAGCAAAGAAATAG---------------
NM_002708.3         AGTGGCCTGAACCCTGGAGGCCGACCCATCACCCCACCCCGCAATTCCGCCAAAGCCAAG
NM_001244974.1      GTAACGCCTCCAAGGGTTGCATCAGGCCTGAACCCGTCCATTCAGAAAGCTTCAAATTAT
NM_001008709.1      AGTGGCCTGAACCCTGGAGGCCGACCCATCACCCCACCCCGCAATTCCGCCAAAGCCAAG
                    . :.  *  ....  * :.    .  ..: ... *. . .   *                

HsCD00004106        AAATAG---------------------
NM_002710.3         ---------------------------
NM_002708.3         AAATAG---------------------
NM_001244974.1      AGAAACAATACTGTTCTATACGAGTGA
NM_001008709.1      AAATAG---------------------
                                               

Pathways

NCI : ALK1 signaling events
NCI : BMP receptor signaling
NCI : TGF-beta receptor signaling
Panther : Dopamine receptor mediated signaling pathway
Panther : Nicotine pharmacodynamics pathway
Reactome : DARPP-32 events
Reactome : Disease
Reactome : Downregulation of TGF-beta receptor signaling
Reactome : Hormone-sensitive lipase (HSL)-mediated triacylglycerol hydrolysis
Reactome : Lipid digestion, mobilization, and transport
Reactome : Loss of Function of SMAD2/3 in Cancer
Reactome : Loss of Function of SMAD4 in Cancer
Reactome : Loss of Function of TGFBR1 in Cancer
Reactome : Loss of Function of TGFBR2 in Cancer
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Opioid Signalling
Reactome : SMAD2/3 MH2 Domain Mutants in Cancer
Reactome : SMAD2/3 Phosphorylation Motif Mutants in Cancer
Reactome : SMAD4 MH2 Domain Mutants in Cancer
Reactome : Signal Transduction
Reactome : Signaling by GPCR
Reactome : Signaling by TGF-beta Receptor Complex
Reactome : Signaling by TGF-beta Receptor Complex in Cancer
Reactome : TGF-beta receptor signaling activates SMADs
Reactome : TGFBR1 KD Mutants in Cancer
Reactome : TGFBR1 LBD Mutants in Cancer
Reactome : TGFBR2 Kinase Domain Mutants in Cancer
Reactome : TGFBR2 MSI Frameshift Mutants in Cancer
WikiPathway : Nicotine Activity on Dopaminergic Neurons

Protein

SMART domain : PP2Ac : Protein phosphatase 2A homologues, catalytic domain.
UniProt : Q07161
UniProt : P62136
UniProt : A6NNR3
HPRD : 15942

Clone

Cloning Information : 26428
HIP Master Clone ID : 56804
Original Clone ID : FLH026428.01X

Organism


TAX_ID : 9606
Species Specific ID: 5499

Genome

Chromosome : 11
Map Location : 11q13
Ensembl : ENSG00000172531

Reagents

Labome : PP1-antibody