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ZNF524 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF524
Gene Name:  zinc finger protein 524
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH184215.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00041169

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 795nts         Open reading frame : 1 to 795
Reference Sequence Alignment

ATGGACACCCCCAGCCCAGACCCGTTGCCTTCGCCTTTGCCCGGGGAGGAAGAGAAACCT
CTGGCCTTATCTCCTCCTGTTCCCCGGGGCCGCCGAGGCCGTCGTCCTGGGGGAGCCACC
TCCTCAAATCGGACACTCAAGGCCTCCCTCCCTCGCAAGCGGGGCCGCCCCCCCAAGTCA
GGGCAGGAGCCCCCACTGGTGCAGGTGCAGGGGGTGACAGCCCCAGTAGGCAGCAGTGGC
GGGAGCGACCTCCTCCTGATCGATGATCAGGGTGTGCCCTATACGGTCTCTGAAGGTTCA
GCGGCTGGGCCTGAGGGCTCTGGCCCCAGGAAGGCCCCACACTTCTGCCCGGTGTGCCTG
CGGGCCTTCCCCTACCTCTCCGACCTGGAGCGCCACAGCATCTCGCACTCAGAGCTGAAG
CCGCACCAGTGCAAGGTTTGCGGCAAGACCTTCAAGCGCTCCAGCCACCTGCGGCGGCAC
TGCAACATCCATGCCGGCCTGCGGCCCTTCCGCTGCCCGCTGTGCCCCCGCCGCTTCCGC
GAGGCGGGCGAGCTGGCGCACCACCACCGCGTGCACTCGGGGGAGCGCCCGTACCAGTGC
CCCATCTGCCGGCTGCGCTTTACAGAGGCCAACACGCTCCGGCGCCATGCGAAGCGCAAG
CACCCGGAGGCCATGGGGGTACCCCTGTGTGCACCAGATCCAGGGTCTGAACCGCCGTGG
GACGAGGAGGGCATCCCGGCCACAGCAGGGGCCGAGGAAGAGGAGGAGACAGAGGGGAAA
GGGGAGCCGGCCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 845
Start on reference sequence 51
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF524
Symbol Nomenclature : ZNF524
Full Nomenclature : zinc finger protein 524
GENEID : 147807
HGNC : 28322
Vega : OTTHUMG00000180859
Target GenBank: BC014666

 

Reference Sequence Alignment

  

HsCD00041169      ATGGACACCCCCAGCCCAGACCCGTTGCCTTCGCCTTTGCCCGGGGAGGAAGAGAAACCT
NM_153219.2       ATGGACACCCCCAGCCCAGACCCGTTGCCTTCGCCTTTGCCCGGGGAGGAAGAGAAACCT
                  ************************************************************

HsCD00041169      CTGGCCTTATCTCCTCCTGTTCCCCGGGGCCGCCGAGGCCGTCGTCCTGGGGGAGCCACC
NM_153219.2       CTGGCCTTATCTCCTCCTGTTCCCCGGGGCCGCCGAGGCCGTCGTCCTGGGGGAGCCACC
                  ************************************************************

HsCD00041169      TCCTCAAATCGGACACTCAAGGCCTCCCTCCCTCGCAAGCGGGGCCGCCCCCCCAAGTCA
NM_153219.2       TCCTCAAATCGGACACTCAAGGCCTCCCTCCCTCGCAAGCGGGGCCGCCCCCCCAAGTCA
                  ************************************************************

HsCD00041169      GGGCAGGAGCCCCCACTGGTGCAGGTGCAGGGGGTGACAGCCCCAGTAGGCAGCAGTGGC
NM_153219.2       GGGCAGGAGCCCCCACTGGTGCAGGTGCAGGGGGTGACAGCCCCAGTAGGCAGCAGTGGC
                  ************************************************************

HsCD00041169      GGGAGCGACCTCCTCCTGATCGATGATCAGGGTGTGCCCTATACGGTCTCTGAAGGTTCA
NM_153219.2       GGGAGCGACCTCCTCCTGATCGATGATCAGGGTGTGCCCTATACGGTCTCTGAAGGTTCA
                  ************************************************************

HsCD00041169      GCGGCTGGGCCTGAGGGCTCTGGCCCCAGGAAGGCCCCACACTTCTGCCCGGTGTGCCTG
NM_153219.2       GCGGCTGGGCCTGAGGGCTCTGGCCCCAGGAAGGCCCCACACTTCTGCCCGGTGTGCCTG
                  ************************************************************

HsCD00041169      CGGGCCTTCCCCTACCTCTCCGACCTGGAGCGCCACAGCATCTCGCACTCAGAGCTGAAG
NM_153219.2       CGGGCCTTCCCCTACCTCTCCGACCTGGAGCGCCACAGCATCTCGCACTCAGAGCTGAAG
                  ************************************************************

HsCD00041169      CCGCACCAGTGCAAGGTTTGCGGCAAGACCTTCAAGCGCTCCAGCCACCTGCGGCGGCAC
NM_153219.2       CCGCACCAGTGCAAGGTTTGCGGCAAGACCTTCAAGCGCTCCAGCCACCTGCGGCGGCAC
                  ************************************************************

HsCD00041169      TGCAACATCCATGCCGGCCTGCGGCCCTTCCGCTGCCCGCTGTGCCCCCGCCGCTTCCGC
NM_153219.2       TGCAACATCCATGCCGGCCTGCGGCCCTTCCGCTGCCCGCTGTGCCCCCGCCGCTTCCGC
                  ************************************************************

HsCD00041169      GAGGCGGGCGAGCTGGCGCACCACCACCGCGTGCACTCGGGGGAGCGCCCGTACCAGTGC
NM_153219.2       GAGGCGGGCGAGCTGGCGCACCACCACCGCGTGCACTCGGGGGAGCGCCCGTACCAGTGC
                  ************************************************************

HsCD00041169      CCCATCTGCCGGCTGCGCTTTACAGAGGCCAACACGCTCCGGCGCCATGCGAAGCGCAAG
NM_153219.2       CCCATCTGCCGGCTGCGCTTTACAGAGGCCAACACGCTCCGGCGCCATGCGAAGCGCAAG
                  ************************************************************

HsCD00041169      CACCCGGAGGCCATGGGGGTACCCCTGTGTGCACCAGATCCAGGGTCTGAACCGCCGTGG
NM_153219.2       CACCCGGAGGCCATGGGGGTACCCCTGTGTGCACCAGATCCAGGGTCTGAACCGCCGTGG
                  ************************************************************

HsCD00041169      GACGAGGAGGGCATCCCGGCCACAGCAGGGGCCGAGGAAGAGGAGGAGACAGAGGGGAAA
NM_153219.2       GACGAGGAGGGCATCCCGGCCACAGCAGGGGCCGAGGAAGAGGAGGAGACAGAGGGGAAA
                  ************************************************************

HsCD00041169      GGGGAGCCGGCCTTG
NM_153219.2       GGGGAGCCGGCCTGA
                  ************* .

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q96C55
HPRD : 15817

Clone

Cloning Information : 184215
HIP Master Clone ID : 159159
Original Clone ID : FLH184215.01L

Organism


TAX_ID : 9606
Species Specific ID: 147807

Genome

Chromosome : 19
Map Location : 19q13.42
Ensembl : ENSG00000171443

Reagents

Labome : ZNF524-antibody