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CNTFR (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CNTFR
Gene Name:  ciliary neurotrophic factor receptor
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH184561.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00041205

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1119nts         Open reading frame : 1 to 1119
Reference Sequence Alignment

ATGGCTGCTCCTGTCCCGTGGGCCTGCTGTGCTGTGCTTGCCGCCGCCGCCGCAGTTGTC
TACGCCCAGAGACACAGTCCACAGGAGGCACCCCATGTGCAGTACGAGCGCCTGGGCTCT
GACGTGACACTGCCATGTGGGACAGCAAACTGGGATGCTGCGGTGACGTGGCGGGTAAAT
GGGACAGACCTGGCCCCTGACCTGCTCAACGGCTCTCAGCTGGTGCTCCATGGCCTGGAA
CTGGGCCACAGTGGCCTCTACGCCTGCTTCCACCGTGACTCCTGGCACCTGCGCCACCAA
GTCCTGCTGCATGTGGGCTTGCCGCCGCGGGAGCCTGTGCTCAGCTGCCGCTCCAACACT
TACCCCAAGGGCTTCTACTGCAGCTGGCATCTGCCCACCCCCACCTACATTCCCAACACC
TTCAATGTGACTGTGCTGCATGGCTCCAAAATTATGGTCTGTGAGAAGGACCCAGCCCTC
AAGAACCGCTGCCACATTCGCTACATGCACCTGTTCTCCACCATCAAGTACAAGGTCTCC
ATAAGTGTCAGCAATGCCCTGGGCCACAATGCCACAGCTATCACCTTTGACGAGTTCACC
ATTGTGAAGCCTGATCCTCCAGAAAATGTGGTAGCCCGGCCAGTGCCCAGCAACCCTCGC
CGGCTGGAGGTGACGTGGCAGACCCCCTCGACCTGGCCTGACCCTGAGTCTTTTCCTCTC
AAGTTCTTTCTGCGCTACCGACCCCTCATCCTGGACCAGTGGCAGCATGTGGAGCTGTCC
GACGGCACAGCACACACCATCACAGATGCCTACGCCGGGAAGGAGTACATTATCCAGGTG
GCAGCCAAGGACAATGAGATTGGGACATGGAGTGACTGGAGCGTAGCCGCCCACGCTACG
CCCTGGACTGAGGAACCGCGACACCTCACCACGGAGGCCCAGGCTGCGGAGACCACGACC
AGCACCACCAGCTCCCTGGCACCCCCACCTACCACGAAGATCTGTGACCCTGGGGAGCTG
GGCAGCGGCGGGGGACCCTCGGCACCCTTCTTGGTCAGCGTCCCCATCACTCTGGCCCTG
GCTGCCGCTGCCGCCACTGCCAGCAGTCTCTTGATCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1268
Start on reference sequence 150
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CNTFR
Symbol Nomenclature : CNTFR
Designation : CNTF receptor subunit alpha|CNTFR-alpha|ciliary neurotrophic factor receptor subunit alpha
Full Nomenclature : ciliary neurotrophic factor receptor
GENEID : 1271
Locus Tag : RP11-296L22.1
HGNC : 2170
MIM : 118946
Vega : OTTHUMG00000019821
Target GenBank: BC001492

 

Reference Sequence Alignment

  

HsCD00041205        ATGGCTGCTCCTGTCCCGTGGGCCTGCTGTGCTGTGCTTGCCGCCGCCGCCGCAGTTGTC
NM_147164.2         ATGGCTGCTCCTGTCCCGTGGGCCTGCTGTGCTGTGCTTGCCGCCGCCGCCGCAGTTGTC
NM_001842.4         ATGGCTGCTCCTGTCCCGTGGGCCTGCTGTGCTGTGCTTGCCGCCGCCGCCGCAGTTGTC
NM_001207011.1      ATGGCTGCTCCTGTCCCGTGGGCCTGCTGTGCTGTGCTTGCCGCCGCCGCCGCAGTTGTC
                    ************************************************************

HsCD00041205        TACGCCCAGAGACACAGTCCACAGGAGGCACCCCATGTGCAGTACGAGCGCCTGGGCTCT
NM_147164.2         TACGCCCAGAGACACAGTCCACAGGAGGCACCCCATGTGCAGTACGAGCGCCTGGGCTCT
NM_001842.4         TACGCCCAGAGACACAGTCCACAGGAGGCACCCCATGTGCAGTACGAGCGCCTGGGCTCT
NM_001207011.1      TACGCCCAGAGACACAGTCCACAGGAGGCACCCCATGTGCAGTACGAGCGCCTGGGCTCT
                    ************************************************************

HsCD00041205        GACGTGACACTGCCATGTGGGACAGCAAACTGGGATGCTGCGGTGACGTGGCGGGTAAAT
NM_147164.2         GACGTGACACTGCCATGTGGGACAGCAAACTGGGATGCTGCGGTGACGTGGCGGGTAAAT
NM_001842.4         GACGTGACACTGCCATGTGGGACAGCAAACTGGGATGCTGCGGTGACGTGGCGGGTAAAT
NM_001207011.1      GACGTGACACTGCCATGTGGGACAGCAAACTGGGATGCTGCGGTGACGTGGCGGGTAAAT
                    ************************************************************

HsCD00041205        GGGACAGACCTGGCCCCTGACCTGCTCAACGGCTCTCAGCTGGTGCTCCATGGCCTGGAA
NM_147164.2         GGGACAGACCTGGCCCCTGACCTGCTCAACGGCTCTCAGCTGGTGCTCCATGGCCTGGAA
NM_001842.4         GGGACAGACCTGGCCCCTGACCTGCTCAACGGCTCTCAGCTGGTGCTCCATGGCCTGGAA
NM_001207011.1      GGGACAGACCTGGCCCCTGACCTGCTCAACGGCTCTCAGCTGGTGCTCCATGGCCTGGAA
                    ************************************************************

HsCD00041205        CTGGGCCACAGTGGCCTCTACGCCTGCTTCCACCGTGACTCCTGGCACCTGCGCCACCAA
NM_147164.2         CTGGGCCACAGTGGCCTCTACGCCTGCTTCCACCGTGACTCCTGGCACCTGCGCCACCAA
NM_001842.4         CTGGGCCACAGTGGCCTCTACGCCTGCTTCCACCGTGACTCCTGGCACCTGCGCCACCAA
NM_001207011.1      CTGGGCCACAGTGGCCTCTACGCCTGCTTCCACCGTGACTCCTGGCACCTGCGCCACCAA
                    ************************************************************

HsCD00041205        GTCCTGCTGCATGTGGGCTTGCCGCCGCGGGAGCCTGTGCTCAGCTGCCGCTCCAACACT
NM_147164.2         GTCCTGCTGCATGTGGGCTTGCCGCCGCGGGAGCCTGTGCTCAGCTGCCGCTCCAACACT
NM_001842.4         GTCCTGCTGCATGTGGGCTTGCCGCCGCGGGAGCCTGTGCTCAGCTGCCGCTCCAACACT
NM_001207011.1      GTCCTGCTGCATGTGGGCTTGCCGCCGCGGGAGCCTGTGCTCAGCTGCCGCTCCAACACT
                    ************************************************************

HsCD00041205        TACCCCAAGGGCTTCTACTGCAGCTGGCATCTGCCCACCCCCACCTACATTCCCAACACC
NM_147164.2         TACCCCAAGGGCTTCTACTGCAGCTGGCATCTGCCCACCCCCACCTACATTCCCAACACC
NM_001842.4         TACCCCAAGGGCTTCTACTGCAGCTGGCATCTGCCCACCCCCACCTACATTCCCAACACC
NM_001207011.1      TACCCCAAGGGCTTCTACTGCAGCTGGCATCTGCCCACCCCCACCTACATTCCCAACACC
                    ************************************************************

HsCD00041205        TTCAATGTGACTGTGCTGCATGGCTCCAAAATTATGGTCTGTGAGAAGGACCCAGCCCTC
NM_147164.2         TTCAATGTGACTGTGCTGCATGGCTCCAAAATTATGGTCTGTGAGAAGGACCCAGCCCTC
NM_001842.4         TTCAATGTGACTGTGCTGCATGGCTCCAAAATTATGGTCTGTGAGAAGGACCCAGCCCTC
NM_001207011.1      TTCAATGTGACTGTGCTGCATGGCTCCAAAATTATGGTCTGTGAGAAGGACCCAGCCCTC
                    ************************************************************

HsCD00041205        AAGAACCGCTGCCACATTCGCTACATGCACCTGTTCTCCACCATCAAGTACAAGGTCTCC
NM_147164.2         AAGAACCGCTGCCACATTCGCTACATGCACCTGTTCTCCACCATCAAGTACAAGGTCTCC
NM_001842.4         AAGAACCGCTGCCACATTCGCTACATGCACCTGTTCTCCACCATCAAGTACAAGGTCTCC
NM_001207011.1      AAGAACCGCTGCCACATTCGCTACATGCACCTGTTCTCCACCATCAAGTACAAGGTCTCC
                    ************************************************************

HsCD00041205        ATAAGTGTCAGCAATGCCCTGGGCCACAATGCCACAGCTATCACCTTTGACGAGTTCACC
NM_147164.2         ATAAGTGTCAGCAATGCCCTGGGCCACAATGCCACAGCTATCACCTTTGACGAGTTCACC
NM_001842.4         ATAAGTGTCAGCAATGCCCTGGGCCACAATGCCACAGCTATCACCTTTGACGAGTTCACC
NM_001207011.1      ATAAGTGTCAGCAATGCCCTGGGCCACAATGCCACAGCTATCACCTTTGACGAGTTCACC
                    ************************************************************

HsCD00041205        ATTGTGAAGCCTGATCCTCCAGAAAATGTGGTAGCCCGGCCAGTGCCCAGCAACCCTCGC
NM_147164.2         ATTGTGAAGCCTGATCCTCCAGAAAATGTGGTAGCCCGGCCAGTGCCCAGCAACCCTCGC
NM_001842.4         ATTGTGAAGCCTGATCCTCCAGAAAATGTGGTAGCCCGGCCAGTGCCCAGCAACCCTCGC
NM_001207011.1      ATTGTGAAGCCTGATCCTCCAGAAAATGTGGTAGCCCGGCCAGTGCCCAGCAACCCTCGC
                    ************************************************************

HsCD00041205        CGGCTGGAGGTGACGTGGCAGACCCCCTCGACCTGGCCTGACCCTGAGTCTTTTCCTCTC
NM_147164.2         CGGCTGGAGGTGACGTGGCAGACCCCCTCGACCTGGCCTGACCCTGAGTCTTTTCCTCTC
NM_001842.4         CGGCTGGAGGTGACGTGGCAGACCCCCTCGACCTGGCCTGACCCTGAGTCTTTTCCTCTC
NM_001207011.1      CGGCTGGAGGTGACGTGGCAGACCCCCTCGACCTGGCCTGACCCTGAGTCTTTTCCTCTC
                    ************************************************************

HsCD00041205        AAGTTCTTTCTGCGCTACCGACCCCTCATCCTGGACCAGTGGCAGCATGTGGAGCTGTCC
NM_147164.2         AAGTTCTTTCTGCGCTACCGACCCCTCATCCTGGACCAGTGGCAGCATGTGGAGCTGTCC
NM_001842.4         AAGTTCTTTCTGCGCTACCGACCCCTCATCCTGGACCAGTGGCAGCATGTGGAGCTGTCC
NM_001207011.1      AAGTTCTTTCTGCGCTACCGACCCCTCATCCTGGACCAGTGGCAGCATGTGGAGCTGTCC
                    ************************************************************

HsCD00041205        GACGGCACAGCACACACCATCACAGATGCCTACGCCGGGAAGGAGTACATTATCCAGGTG
NM_147164.2         GACGGCACAGCACACACCATCACAGATGCCTACGCCGGGAAGGAGTACATTATCCAGGTG
NM_001842.4         GACGGCACAGCACACACCATCACAGATGCCTACGCCGGGAAGGAGTACATTATCCAGGTG
NM_001207011.1      GACGGCACAGCACACACCATCACAGATGCCTACGCCGGGAAGGAGTACATTATCCAGGTG
                    ************************************************************

HsCD00041205        GCAGCCAAGGACAATGAGATTGGGACATGGAGTGACTGGAGCGTAGCCGCCCACGCTACG
NM_147164.2         GCAGCCAAGGACAATGAGATTGGGACATGGAGTGACTGGAGCGTAGCCGCCCACGCTACG
NM_001842.4         GCAGCCAAGGACAATGAGATTGGGACATGGAGTGACTGGAGCGTAGCCGCCCACGCTACG
NM_001207011.1      GCAGCCAAGGACAATGAGATTGGGACATGGAGTGACTGGAGCGTAGCCGCCCACGCTACG
                    ************************************************************

HsCD00041205        CCCTGGACTGAGGAACCGCGACACCTCACCACGGAGGCCCAGGCTGCGGAGACCACGACC
NM_147164.2         CCCTGGACTGAGGAACCGCGACACCTCACCACGGAGGCCCAGGCTGCGGAGACCACGACC
NM_001842.4         CCCTGGACTGAGGAACCGCGACACCTCACCACGGAGGCCCAGGCTGCGGAGACCACGACC
NM_001207011.1      CCCTGGACTGAGGAACCGCGACACCTCACCACGGAGGCCCAGGCTGCGGAGACCACGACC
                    ************************************************************

HsCD00041205        AGCACCACCAGCTCCCTGGCACCCCCACCTACCACGAAGATCTGTGACCCTGGGGAGCTG
NM_147164.2         AGCACCACCAGCTCCCTGGCACCCCCACCTACCACGAAGATCTGTGACCCTGGGGAGCTG
NM_001842.4         AGCACCACCAGCTCCCTGGCACCCCCACCTACCACGAAGATCTGTGACCCTGGGGAGCTG
NM_001207011.1      AGCACCACCAGCTCCCTGGCACCCCCACCTACCACGAAGATCTGTGACCCTGGGGAGCTG
                    ************************************************************

HsCD00041205        GGCAGCGGCGGGGGACCCTCGGCACCCTTCTTGGTCAGCGTCCCCATCACTCTGGCCCTG
NM_147164.2         GGCAGCGGCGGGGGACCCTCGGCACCCTTCTTGGTCAGCGTCCCCATCACTCTGGCCCTG
NM_001842.4         GGCAGCGGCGGGGGACCCTCGGCACCCTTCTTGGTCAGCGTCCCCATCACTCTGGCCCTG
NM_001207011.1      GGCAGCGGCGGGGGACCCTCGGCACCCTTCTTGGTCAGCGTCCCCATCACTCTGGCCCTG
                    ************************************************************

HsCD00041205        GCTGCCGCTGCCGCCACTGCCAGCAGTCTCTTGATCTTG
NM_147164.2         GCTGCCGCTGCCGCCACTGCCAGCAGTCTCTTGATCTGA
NM_001842.4         GCTGCCGCTGCCGCCACTGCCAGCAGTCTCTTGATCTGA
NM_001207011.1      GCTGCCGCTGCCGCCACTGCCAGCAGTCTCTTGATCTGA
                    ************************************* .

Pathways

WikiPathway : Adipogenesis

Protein

SMART domain : IG : Immunoglobulin
SMART domain : FN3 : Fibronectin type 3 domain
SMART domain : IGc2 : Immunoglobulin C-2 Type
UniProt : P26992
UniProt : Q5U050
HPRD : 00348

Clone

Cloning Information : 184561
HIP Master Clone ID : 159505
Original Clone ID : FLH184561.01L

Organism


TAX_ID : 9606
Species Specific ID: 1271

Genome

Chromosome : 9
Map Location : 9p13
Ensembl : ENSG00000122756

Reagents

Labome : CNTFR-antibody