with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, bacterial expression expression with pCPD5698_F and pCPD6268_R and MBP and Thrombin and SBP and TEV and ccdB and CmR and T7 term and LacI and AmpR and ColE and T7 and lac operator and RBS; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial;
Comments:
None
Publications:
None
Authors:
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, bacterial expression expression with pCPD5698_F and pCPD6268_R and MBP and Thrombin and SBP and TEV and ccdB and CmR and T7 term and LacI and AmpR and ColE and T7 and lac operator and RBS; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial;
Comments:
One in a series of pCPD vectors made by J. Saul for E. coli protein expression at CPD.
Size (bp):
8193
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 replication origin
1344
2026
death cassette
ccdB
ccdB death cassette (removed in clones with insert)