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GPR17 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GPR17
Gene Name:  G protein-coupled receptor 17
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH186461.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00041415

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1020nts         Open reading frame : 1 to 1020
Reference Sequence Alignment

ATGAATGGCCTTGAAGTGGCTCCCCCAGGTCTGATCACCAACTTCTCCCTGGCCACGGCA
GAGCAATGTGGCCAGGAGACGCCACTGGAGAACATGCTGTTCGCCTCCTTCTACCTTCTA
GATTTTATCCTGGCTTTAGTTGGCAATACCCTGGCTCTGTGGTTTTTCATCCGAGACCAC
AAGTCCGGGACCCCGGCCAACGTGTTCCTGATGCATCTGGCCGTGGCCGACTTGTCGTGC
GTGCTGGTCCTGCCCACCCGCCTGGTCTACCACTTCTCTGGGAACCACTGGCCATTTGGG
GAAATCGCATGCCGTCTCACCGGCTTCCTCTTCTACCTCAACATGTACGCCAGCATCTAC
TTCCTCACCTGCATCAGCGCCGACCGTTTCCTGGCCATTGTGCACCCGGTCAAGTCCCTC
AAGCTCCGCAGGCCCCTCTACGCACACCTGGCCTGTGCCTTCCTGTGGGTGGTGGTGGCT
GTGGCCATGGCCCCGCTGCTGGTGAGCCCACAGACCGTGCAGACCAACCACACGGTGGTC
TGCCTGCAGCTGTACCGGGAGAAGGCCTCCCACCATGCCCTGGTGTCCCTGGCAGTGGCC
TTCACCTTCCCGTTCATCACCACGGTCACCTGCTACCTGCTGATCATCCGCAGCCTGCGG
CAGGGCCTGCGTGTGGAGAAGCGCCTCAAGACCAAGGCAGTGCGCATGATCGCCATAGTG
CTGGCCATCTTCCTGGTCTGCTTCGTGCCCTACCACGTCAACCGCTCCGTCTACGTGCTG
CACTACCGCAGCCATGGGGCCTCCTGCGCCACCCAGCGCATCCTGGCCCTGGCAAACCGC
ATCACCTCCTGCCTCACCAGCCTCAACGGGGCACTCGACCCCATCATGTATTTCTTCGTG
GCTGAGAAGTTCCGCCACGCCCTGTGCAACTTGCTCTGTGGCAAAAGGCTCAAGGGCCCG
CCCCCCAGCTTCGAAGGGAAAACCAACGAGAGCTCGCTGAGTGCCAAGTCAGAGCTGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1100
Start on reference sequence 81
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GPR17
Symbol Nomenclature : GPR17
Designation : G-protein coupled receptor 17|P2Y-like receptor|R12|UDP/CysLT receptor|uracil nucleotide/cysteinyl leukotriene receptor
Full Nomenclature : G protein-coupled receptor 17
GENEID : 2840
HGNC : 4471
MIM : 603071
Vega : OTTHUMG00000131533
Target GenBank: BC031653

 

Reference Sequence Alignment

  

HsCD00041415        ------------------------------------------------------------
NM_001161416.1      ------------------------------------------------------------
NM_001161415.1      ATGTCCAAACGGAGTTGGTGGGCTGGATCCAGAAAGCCCCCAAGAGAGATGCTGAAACTC
NM_001161417.1      ------------------------------------------------------------
NM_005291.2         ATGTCCAAACGGAGTTGGTGGGCTGGATCCAGAAAGCCCCCAAGAGAGATGCTGAAACTC
                                                                                

HsCD00041415        ------------------------ATGAATGGCCTTGAAGTGGCTCCCCCAGGTCTGATC
NM_001161416.1      ------------------------ATGAATGGCCTTGAAGTGGCTCCCCCAGGTCTGATC
NM_001161415.1      TCAGGCTCTGACTCCAGCCAAAGCATGAATGGCCTTGAAGTGGCTCCCCCAGGTCTGATC
NM_001161417.1      ------------------------ATGAATGGCCTTGAAGTGGCTCCCCCAGGTCTGATC
NM_005291.2         TCAGGCTCTGACTCCAGCCAAAGCATGAATGGCCTTGAAGTGGCTCCCCCAGGTCTGATC
                                            ************************************

HsCD00041415        ACCAACTTCTCCCTGGCCACGGCAGAGCAATGTGGCCAGGAGACGCCACTGGAGAACATG
NM_001161416.1      ACCAACTTCTCCCTGGCCACGGCAGAGCAATGTGGCCAGGAGACGCCACTGGAGAACATG
NM_001161415.1      ACCAACTTCTCCCTGGCCACGGCAGAGCAATGTGGCCAGGAGACGCCACTGGAGAACATG
NM_001161417.1      ACCAACTTCTCCCTGGCCACGGCAGAGCAATGTGGCCAGGAGACGCCACTGGAGAACATG
NM_005291.2         ACCAACTTCTCCCTGGCCACGGCAGAGCAATGTGGCCAGGAGACGCCACTGGAGAACATG
                    ************************************************************

HsCD00041415        CTGTTCGCCTCCTTCTACCTTCTAGATTTTATCCTGGCTTTAGTTGGCAATACCCTGGCT
NM_001161416.1      CTGTTCGCCTCCTTCTACCTTCTGGATTTTATCCTGGCTTTAGTTGGCAATACCCTGGCT
NM_001161415.1      CTGTTCGCCTCCTTCTACCTTCTGGATTTTATCCTGGCTTTAGTTGGCAATACCCTGGCT
NM_001161417.1      CTGTTCGCCTCCTTCTACCTTCTGGATTTTATCCTGGCTTTAGTTGGCAATACCCTGGCT
NM_005291.2         CTGTTCGCCTCCTTCTACCTTCTGGATTTTATCCTGGCTTTAGTTGGCAATACCCTGGCT
                    ***********************.************************************

HsCD00041415        CTGTGGTTTTTCATCCGAGACCACAAGTCCGGGACCCCGGCCAACGTGTTCCTGATGCAT
NM_001161416.1      CTGTGGCTTTTCATCCGAGACCACAAGTCCGGGACCCCGGCCAACGTGTTCCTGATGCAT
NM_001161415.1      CTGTGGCTTTTCATCCGAGACCACAAGTCCGGGACCCCGGCCAACGTGTTCCTGATGCAT
NM_001161417.1      CTGTGGCTTTTCATCCGAGACCACAAGTCCGGGACCCCGGCCAACGTGTTCCTGATGCAT
NM_005291.2         CTGTGGCTTTTCATCCGAGACCACAAGTCCGGGACCCCGGCCAACGTGTTCCTGATGCAT
                    ****** *****************************************************

HsCD00041415        CTGGCCGTGGCCGACTTGTCGTGCGTGCTGGTCCTGCCCACCCGCCTGGTCTACCACTTC
NM_001161416.1      CTGGCCGTGGCCGACTTGTCGTGCGTGCTGGTCCTGCCCACCCGCCTGGTCTACCACTTC
NM_001161415.1      CTGGCCGTGGCCGACTTGTCGTGCGTGCTGGTCCTGCCCACCCGCCTGGTCTACCACTTC
NM_001161417.1      CTGGCCGTGGCCGACTTGTCGTGCGTGCTGGTCCTGCCCACCCGCCTGGTCTACCACTTC
NM_005291.2         CTGGCCGTGGCCGACTTGTCGTGCGTGCTGGTCCTGCCCACCCGCCTGGTCTACCACTTC
                    ************************************************************

HsCD00041415        TCTGGGAACCACTGGCCATTTGGGGAAATCGCATGCCGTCTCACCGGCTTCCTCTTCTAC
NM_001161416.1      TCTGGGAACCACTGGCCATTTGGGGAAATCGCATGCCGTCTCACCGGCTTCCTCTTCTAC
NM_001161415.1      TCTGGGAACCACTGGCCATTTGGGGAAATCGCATGCCGTCTCACCGGCTTCCTCTTCTAC
NM_001161417.1      TCTGGGAACCACTGGCCATTTGGGGAAATCGCATGCCGTCTCACCGGCTTCCTCTTCTAC
NM_005291.2         TCTGGGAACCACTGGCCATTTGGGGAAATCGCATGCCGTCTCACCGGCTTCCTCTTCTAC
                    ************************************************************

HsCD00041415        CTCAACATGTACGCCAGCATCTACTTCCTCACCTGCATCAGCGCCGACCGTTTCCTGGCC
NM_001161416.1      CTCAACATGTACGCCAGCATCTACTTCCTCACCTGCATCAGCGCCGACCGTTTCCTGGCC
NM_001161415.1      CTCAACATGTACGCCAGCATCTACTTCCTCACCTGCATCAGCGCCGACCGTTTCCTGGCC
NM_001161417.1      CTCAACATGTACGCCAGCATCTACTTCCTCACCTGCATCAGCGCCGACCGTTTCCTGGCC
NM_005291.2         CTCAACATGTACGCCAGCATCTACTTCCTCACCTGCATCAGCGCCGACCGTTTCCTGGCC
                    ************************************************************

HsCD00041415        ATTGTGCACCCGGTCAAGTCCCTCAAGCTCCGCAGGCCCCTCTACGCACACCTGGCCTGT
NM_001161416.1      ATTGTGCACCCGGTCAAGTCCCTCAAGCTCCGCAGGCCCCTCTACGCACACCTGGCCTGT
NM_001161415.1      ATTGTGCACCCGGTCAAGTCCCTCAAGCTCCGCAGGCCCCTCTACGCACACCTGGCCTGT
NM_001161417.1      ATTGTGCACCCGGTCAAGTCCCTCAAGCTCCGCAGGCCCCTCTACGCACACCTGGCCTGT
NM_005291.2         ATTGTGCACCCGGTCAAGTCCCTCAAGCTCCGCAGGCCCCTCTACGCACACCTGGCCTGT
                    ************************************************************

HsCD00041415        GCCTTCCTGTGGGTGGTGGTGGCTGTGGCCATGGCCCCGCTGCTGGTGAGCCCACAGACC
NM_001161416.1      GCCTTCCTGTGGGTGGTGGTGGCTGTGGCCATGGCCCCGCTGCTGGTGAGCCCACAGACC
NM_001161415.1      GCCTTCCTGTGGGTGGTGGTGGCTGTGGCCATGGCCCCGCTGCTGGTGAGCCCACAGACC
NM_001161417.1      GCCTTCCTGTGGGTGGTGGTGGCTGTGGCCATGGCCCCGCTGCTGGTGAGCCCACAGACC
NM_005291.2         GCCTTCCTGTGGGTGGTGGTGGCTGTGGCCATGGCCCCGCTGCTGGTGAGCCCACAGACC
                    ************************************************************

HsCD00041415        GTGCAGACCAACCACACGGTGGTCTGCCTGCAGCTGTACCGGGAGAAGGCCTCCCACCAT
NM_001161416.1      GTGCAGACCAACCACACGGTGGTCTGCCTGCAGCTGTACCGGGAGAAGGCCTCCCACCAT
NM_001161415.1      GTGCAGACCAACCACACGGTGGTCTGCCTGCAGCTGTACCGGGAGAAGGCCTCCCACCAT
NM_001161417.1      GTGCAGACCAACCACACGGTGGTCTGCCTGCAGCTGTACCGGGAGAAGGCCTCCCACCAT
NM_005291.2         GTGCAGACCAACCACACGGTGGTCTGCCTGCAGCTGTACCGGGAGAAGGCCTCCCACCAT
                    ************************************************************

HsCD00041415        GCCCTGGTGTCCCTGGCAGTGGCCTTCACCTTCCCGTTCATCACCACGGTCACCTGCTAC
NM_001161416.1      GCCCTGGTGTCCCTGGCAGTGGCCTTCACCTTCCCGTTCATCACCACGGTCACCTGCTAC
NM_001161415.1      GCCCTGGTGTCCCTGGCAGTGGCCTTCACCTTCCCGTTCATCACCACGGTCACCTGCTAC
NM_001161417.1      GCCCTGGTGTCCCTGGCAGTGGCCTTCACCTTCCCGTTCATCACCACGGTCACCTGCTAC
NM_005291.2         GCCCTGGTGTCCCTGGCAGTGGCCTTCACCTTCCCGTTCATCACCACGGTCACCTGCTAC
                    ************************************************************

HsCD00041415        CTGCTGATCATCCGCAGCCTGCGGCAGGGCCTGCGTGTGGAGAAGCGCCTCAAGACCAAG
NM_001161416.1      CTGCTGATCATCCGCAGCCTGCGGCAGGGCCTGCGTGTGGAGAAGCGCCTCAAGACCAAG
NM_001161415.1      CTGCTGATCATCCGCAGCCTGCGGCAGGGCCTGCGTGTGGAGAAGCGCCTCAAGACCAAG
NM_001161417.1      CTGCTGATCATCCGCAGCCTGCGGCAGGGCCTGCGTGTGGAGAAGCGCCTCAAGACCAAG
NM_005291.2         CTGCTGATCATCCGCAGCCTGCGGCAGGGCCTGCGTGTGGAGAAGCGCCTCAAGACCAAG
                    ************************************************************

HsCD00041415        GCAGTGCGCATGATCGCCATAGTGCTGGCCATCTTCCTGGTCTGCTTCGTGCCCTACCAC
NM_001161416.1      GCAGTGCGCATGATCGCCATAGTGCTGGCCATCTTCCTGGTCTGCTTCGTGCCCTACCAC
NM_001161415.1      GCAGTGCGCATGATCGCCATAGTGCTGGCCATCTTCCTGGTCTGCTTCGTGCCCTACCAC
NM_001161417.1      GCAGTGCGCATGATCGCCATAGTGCTGGCCATCTTCCTGGTCTGCTTCGTGCCCTACCAC
NM_005291.2         GCAGTGCGCATGATCGCCATAGTGCTGGCCATCTTCCTGGTCTGCTTCGTGCCCTACCAC
                    ************************************************************

HsCD00041415        GTCAACCGCTCCGTCTACGTGCTGCACTACCGCAGCCATGGGGCCTCCTGCGCCACCCAG
NM_001161416.1      GTCAACCGCTCCGTCTACGTGCTGCACTACCGCAGCCATGGGGCCTCCTGCGCCACCCAG
NM_001161415.1      GTCAACCGCTCCGTCTACGTGCTGCACTACCGCAGCCATGGGGCCTCCTGCGCCACCCAG
NM_001161417.1      GTCAACCGCTCCGTCTACGTGCTGCACTACCGCAGCCATGGGGCCTCCTGCGCCACCCAG
NM_005291.2         GTCAACCGCTCCGTCTACGTGCTGCACTACCGCAGCCATGGGGCCTCCTGCGCCACCCAG
                    ************************************************************

HsCD00041415        CGCATCCTGGCCCTGGCAAACCGCATCACCTCCTGCCTCACCAGCCTCAACGGGGCACTC
NM_001161416.1      CGCATCCTGGCCCTGGCAAACCGCATCACCTCCTGCCTCACCAGCCTCAACGGGGCACTC
NM_001161415.1      CGCATCCTGGCCCTGGCAAACCGCATCACCTCCTGCCTCACCAGCCTCAACGGGGCACTC
NM_001161417.1      CGCATCCTGGCCCTGGCAAACCGCATCACCTCCTGCCTCACCAGCCTCAACGGGGCACTC
NM_005291.2         CGCATCCTGGCCCTGGCAAACCGCATCACCTCCTGCCTCACCAGCCTCAACGGGGCACTC
                    ************************************************************

HsCD00041415        GACCCCATCATGTATTTCTTCGTGGCTGAGAAGTTCCGCCACGCCCTGTGCAACTTGCTC
NM_001161416.1      GACCCCATCATGTATTTCTTCGTGGCTGAGAAGTTCCGCCACGCCCTGTGCAACTTGCTC
NM_001161415.1      GACCCCATCATGTATTTCTTCGTGGCTGAGAAGTTCCGCCACGCCCTGTGCAACTTGCTC
NM_001161417.1      GACCCCATCATGTATTTCTTCGTGGCTGAGAAGTTCCGCCACGCCCTGTGCAACTTGCTC
NM_005291.2         GACCCCATCATGTATTTCTTCGTGGCTGAGAAGTTCCGCCACGCCCTGTGCAACTTGCTC
                    ************************************************************

HsCD00041415        TGTGGCAAAAGGCTCAAGGGCCCGCCCCCCAGCTTCGAAGGGAAAACCAACGAGAGCTCG
NM_001161416.1      TGTGGCAAAAGGCTCAAGGGCCCGCCCCCCAGCTTCGAAGGGAAAACCAACGAGAGCTCG
NM_001161415.1      TGTGGCAAAAGGCTCAAGGGCCCGCCCCCCAGCTTCGAAGGGAAAACCAACGAGAGCTCG
NM_001161417.1      TGTGGCAAAAGGCTCAAGGGCCCGCCCCCCAGCTTCGAAGGGAAAACCAACGAGAGCTCG
NM_005291.2         TGTGGCAAAAGGCTCAAGGGCCCGCCCCCCAGCTTCGAAGGGAAAACCAACGAGAGCTCG
                    ************************************************************

HsCD00041415        CTGAGTGCCAAGTCAGAGCTGTTG
NM_001161416.1      CTGAGTGCCAAGTCAGAGCTGTGA
NM_001161415.1      CTGAGTGCCAAGTCAGAGCTGTGA
NM_001161417.1      CTGAGTGCCAAGTCAGAGCTGTGA
NM_005291.2         CTGAGTGCCAAGTCAGAGCTGTGA
                    ********************** .

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : Eicosanoid ligand-binding receptors
Reactome : G alpha (i) signalling events
Reactome : G alpha (q) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Gastrin-CREB signalling pathway via PKC and MAPK
Reactome : Leukotriene receptors
Reactome : Nucleotide-like (purinergic) receptors
Reactome : P2Y receptors
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class A Rhodopsin-like
WikiPathway : GPCRs, Other

Protein

UniProt : Q13304
HPRD : 04351

Clone

Cloning Information : 186461
HIP Master Clone ID : 161405
Original Clone ID : FLH186461.01L

Organism


TAX_ID : 9606
Species Specific ID: 2840

Genome

Chromosome : 2
Map Location : 2q21
Ensembl : ENSG00000144230

Reagents

Labome : GPR17-antibody