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COQ2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  COQ2
Gene Name:  coenzyme Q2 4-hydroxybenzoate polyprenyltransferase
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH188854.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00041667

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1116nts         Open reading frame : 1 to 1116
Reference Sequence Alignment

ATGCTGGGCTCGCGAGCCGCGGGGTTCGCGCGGGGCCTGCGGGCTTTGGCACTGGCGTGG
CTGCCGGGCTGGCGGGGCCGCTCCTTCGCCCTGGCGCGTGCGGCAGGCGCGCCCCACGGT
GGTGACTTGCAGCCCCCCGCCTGTCCCGAGCCGCGCGGGCGCCAGCTCAGTTTGTCCGCG
GCGGCGGTGGTGGACTCTGCGCCCCGCCCCCTGCAGCCGTACTTGCGCCTCATGCGGTTG
GACAAGCCCATTGGAACCTGGCTTCTGTATTTACCATGTACCTGGAGCATTGGTTTGGCA
GCTGAACCAGGTTGTTTTCCAGATTGGTACATGCTCTCCCTCTTTGGCACTGGAGCTATT
CTGATGCGTGGAGCAGGCTGTACTATTAATGACATGTGGGACCAGGACTATGATAAAAAG
GTTACAAGAACAGCCAATCGTCCAATAGCCGCTGGAGACATTTCAACTTTTCAGTCCTTT
GTTTTTCTTGGGGGACAGCTAACCCTGGCACTGGGTGTTCTTCTGTGTCTAAATTACTAC
AGTATAGCTCTGGGAGCAGGATCCTTACTTCTTGTCATCACCTACCCACTAATGAAAAGA
ATTTCATACTGGCCTCAACTAGCCTTGGGCTTGACATTTAATTGGGGAGCGTTACTTGGA
TGGTCTGCTATCAAGGGTTCCTGTGATCCATCTGTTTGCCTGCCTCTTTATTTTTCTGGA
GTTATGTGGACACTAATATATGACACTATTTATGCCCATCAGGACAAAAGAGATGATGTT
TTGATTGGTCTTAAGTCAACGGCTCTGCGGTTCGGAGAAAATACCAAGCCGTGGCTCAGC
GGCTTCAGTGTTGCAATGCTGGGGGCACTGAGCCTAGTGGGTGTGAACAGTGGACAGACT
GCTCCCTACTACGCTGCCCTGGGTGCTGTAGGAGCCCATCTGACTCACCAGATTTACACT
CTAGACATCCACAGACCTGAGGATTGTTGGAATAAATTTATCTCCAACCGAACACTGGGA
CTAATAGTTTTTTTAGGGATTGTCCTTGGGAATTTGTGGAAAGAAAAGAAGACAGACAAA
ACAAAGAAGGGTATAGAGAATAAAATAGAAAATTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1132
Start on reference sequence 17
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : COQ2
Symbol Nomenclature : COQ2
SYNONYM : CL640; COQ10D1; MSA1
Designation : 4-hydroxybenzoate polyprenyltransferase, mitochondrial|PHB:polyprenyltransferase|coenzyme Q2 homolog, prenyltransferase|para-hydroxybenzoate-polyprenyltransferase, mitochondrial
Full Nomenclature : coenzyme Q2 4-hydroxybenzoate polyprenyltransferase
GENEID : 27235
HGNC : 25223
MIM : 609825
Vega : OTTHUMG00000160940
Target GenBank: BC008804

 

Reference Sequence Alignment

  

HsCD00041667      ------------------------------------------------------------
NM_015697.7       ATGACCCCAATTTCACAAGTAAGGATGAGGAAAGGTTCTGCCCACACCGCCGCCCAGCCT
                                                                              

HsCD00041667      ------------------------------------------------------------
NM_015697.7       GGCAGACTAGGATTGCATCCTGCGGGTGCCACTGCGCATGCCTGCCGGGGAATGACGTCA
                                                                              

HsCD00041667      ------------------------------ATGCTGGGCTCGCGAGCCGCGGGGTTCGCG
NM_015697.7       ATCCGAGCTCGTCCCGGCCTCACCAGCGCCATGCTGGGCTCGCGAGCCGCGGGGTTCGCG
                                                ******************************

HsCD00041667      CGGGGCCTGCGGGCTTTGGCACTGGCGTGGCTGCCGGGCTGGCGGGGCCGCTCCTTCGCC
NM_015697.7       CGGGGCCTGCGGGCTGTGGCACTGGCGTGGCTGCCGGGCTGGCGGGGCCGCTCCTTCGCC
                  *************** ********************************************

HsCD00041667      CTGGCGCGTGCGGCAGGCGCGCCCCACGGTGGTGACTTGCAGCCCCCCGCCTGTCCCGAG
NM_015697.7       CTGGCGCGTGCGGCAGGCGCGCCCCACGGTGGTGACTTGCAGCCCCCCGCCTGTCCCGAG
                  ************************************************************

HsCD00041667      CCGCGCGGGCGCCAGCTCAGTTTGTCCGCGGCGGCGGTGGTGGACTCTGCGCCCCGCCCC
NM_015697.7       CCGCGCGGGCGCCAGCTCAGTTTGTCCGCGGCGGCGGTGGTGGACTCTGCGCCCCGCCCC
                  ************************************************************

HsCD00041667      CTGCAGCCGTACTTGCGCCTCATGCGGTTGGACAAGCCCATTGGAACCTGGCTTCTGTAT
NM_015697.7       CTGCAGCCGTACTTGCGCCTCATGCGGTTGGACAAGCCCATTGGAACCTGGCTTCTGTAT
                  ************************************************************

HsCD00041667      TTACCATGTACCTGGAGCATTGGTTTGGCAGCTGAACCAGGTTGTTTTCCAGATTGGTAC
NM_015697.7       TTACCATGTACCTGGAGCATTGGTTTGGCAGCTGAACCAGGTTGTTTTCCAGATTGGTAC
                  ************************************************************

HsCD00041667      ATGCTCTCCCTCTTTGGCACTGGAGCTATTCTGATGCGTGGAGCAGGCTGTACTATTAAT
NM_015697.7       ATGCTCTCCCTCTTTGGCACTGGAGCTATTCTGATGCGTGGAGCAGGCTGTACTATTAAT
                  ************************************************************

HsCD00041667      GACATGTGGGACCAGGACTATGATAAAAAGGTTACAAGAACAGCCAATCGTCCAATAGCC
NM_015697.7       GACATGTGGGACCAGGACTATGATAAAAAGGTTACAAGAACAGCCAATCGTCCAATAGCC
                  ************************************************************

HsCD00041667      GCTGGAGACATTTCAACTTTTCAGTCCTTTGTTTTTCTTGGGGGACAGCTAACCCTGGCA
NM_015697.7       GCTGGAGACATTTCAACTTTTCAGTCCTTTGTTTTTCTTGGGGGACAGCTAACCCTGGCA
                  ************************************************************

HsCD00041667      CTGGGTGTTCTTCTGTGTCTAAATTACTACAGTATAGCTCTGGGAGCAGGATCCTTACTT
NM_015697.7       CTGGGTGTTCTTCTGTGTCTAAATTACTACAGTATAGCTCTGGGAGCAGGATCCTTACTT
                  ************************************************************

HsCD00041667      CTTGTCATCACCTACCCACTAATGAAAAGAATTTCATACTGGCCTCAACTAGCCTTGGGC
NM_015697.7       CTTGTCATCACCTACCCACTAATGAAAAGAATTTCATACTGGCCTCAACTAGCCTTGGGC
                  ************************************************************

HsCD00041667      TTGACATTTAATTGGGGAGCGTTACTTGGATGGTCTGCTATCAAGGGTTCCTGTGATCCA
NM_015697.7       TTGACATTTAATTGGGGAGCGTTACTTGGATGGTCTGCTATCAAGGGTTCCTGTGATCCA
                  ************************************************************

HsCD00041667      TCTGTTTGCCTGCCTCTTTATTTTTCTGGAGTTATGTGGACACTAATATATGACACTATT
NM_015697.7       TCTGTTTGCCTGCCTCTTTATTTTTCTGGAGTTATGTGGACACTAATATATGATACTATT
                  ***************************************************** ******

HsCD00041667      TATGCCCATCAGGACAAAAGAGATGATGTTTTGATTGGTCTTAAGTCAACGGCTCTGCGG
NM_015697.7       TATGCCCATCAGGACAAAAGAGATGATGTTTTGATTGGTCTTAAGTCAACGGCTCTGCGG
                  ************************************************************

HsCD00041667      TTCGGAGAAAATACCAAGCCGTGGCTCAGCGGCTTCAGTGTTGCAATGCTGGGGGCACTG
NM_015697.7       TTCGGAGAAAATACCAAGCCGTGGCTCAGCGGCTTCAGTGTTGCAATGCTGGGGGCACTG
                  ************************************************************

HsCD00041667      AGCCTAGTGGGTGTGAACAGTGGACAGACTGCTCCCTACTACGCTGCCCTGGGTGCTGTA
NM_015697.7       AGCCTAGTGGGTGTGAACAGTGGACAGACTGCTCCCTACTACGCTGCCCTGGGTGCTGTA
                  ************************************************************

HsCD00041667      GGAGCCCATCTGACTCACCAGATTTACACTCTAGACATCCACAGACCTGAGGATTGTTGG
NM_015697.7       GGAGCCCATCTGACTCACCAGATTTACACTCTAGACATCCACAGACCTGAGGATTGTTGG
                  ************************************************************

HsCD00041667      AATAAATTTATCTCCAACCGAACACTGGGACTAATAGTTTTTTTAGGGATTGTCCTTGGG
NM_015697.7       AATAAATTTATCTCCAACCGAACACTGGGACTAATAGTTTTTTTAGGGATTGTCCTTGGG
                  ************************************************************

HsCD00041667      AATTTGTGGAAAGAAAAGAAGACAGACAAAACAAAGAAGGGTATAGAGAATAAAATAGAA
NM_015697.7       AATTTGTGGAAAGAAAAGAAGACAGACAAAACAAAGAAGGGTATAGAGAATAAAATAGAA
                  ************************************************************

HsCD00041667      AATTTG
NM_015697.7       AATTAA
                  ****:.

Pathways

Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Metabolism of proteins
Reactome : Mitochondrial protein import
Reactome : Ubiquinol biosynthesis

Protein

UniProt : Q96H96
HPRD : 13062

Clone

Cloning Information : 188854
HIP Master Clone ID : 163798
Original Clone ID : FLH188854.01L

Organism


TAX_ID : 9606
Species Specific ID: 27235

Genome

Chromosome : 4
Map Location : 4q21.23
Ensembl : ENSG00000173085

Reagents

Labome : COQ2-antibody