DNASU Plasmid Repository • 480.965.5697 | Email

TOMM40 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  TOMM40
Gene Name:  translocase of outer mitochondrial membrane 40 homolog (yeast)
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH191000.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00041926

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1086nts         Open reading frame : 1 to 1086
Reference Sequence Alignment

ATGGGGAACGTGTTGGCTGCCAGCTCGCCGCCCGCAGGGCCGCCACCGCCGCCTGCGCCG
GCCCTCGTGGGGCTGCCGCCACCTCCGCCCTCGCCGCCGGGCTTCACGCTGCCGCCGCTG
GGAGGCAGCCTGGGCGCCGGCACCAGTACGAGTCGAAGTTCGGAACGGACCCCCGGGGCT
GCAACCGCCAGCGCCTCAGGGGCCGCCGAGGATGGGGCCTGCGGCTGCCTGCCCAACCCG
GGCACATTCGAGGAGTGCCACCGGAAGTGCAAGGAGCTGTTTCCCATTCAGATGGAGGGT
GTCAAGCTCACAGTCAACAAAGGGTTGAGTAACCATTTTCAGGTCAACCACACAGTAGCC
CTCAGCACAATCGGGGAGTCCAACTACCACTTCGGGGTCACATATGTGGGGACAAAGCAG
CTGAGTCCCACAGAGGCGTTCCCTGTACTGGTGGGTGACATGGACAACAGTGGCAGTCTC
AACGCTCAGGTCATTCACCAGCTGGGCCCCGGTCTCAGGTCCAAGATGGCCATCCAGACC
CAGCAGTCGAAGTTTGTGAACTGGCAGGTGGACGGGGAGTATCGGGGCTCTGACTTCACA
GCAGCCGTCACCCTGGGGAACCCAGACGTCCTCGTGGGTTCAGGAATCCTCGTAGCCCAC
TACCTCCAGAGCATCACGCCTTGCCTGGCCCTGGGTGGAGAGCTGGTCTACCACCGGCGG
CCTGGAGAGGAGGGCACTGTCATGTCTCTAGCTGGGAAATACACATTGAACAACTGGTTG
GCAACGGTAACGTTGGGCCAGGCGGGCATGCACGCAACATACTACCACAAAGCCAGTGAC
CAGCTGCAGGTGGGTGTGGAGTTTGAGGCCAGCACAAGGATGCAGGACACCAGCGTCTCC
TTCGGGTACCAGCTGGACCTGCCCAAGGCCAACCTCCTCTTCAAAGGCTCTGTGGATAGC
AACTGGATCGTGGGTGCCACGCTGGAGAAGAAGCTCCCACCCCTGCCCCTGACACTGGCC
CTTGGGGCCTTCCTGAATCACCGCAAGAACAAGTTTCAGTGTGGCTTTGGCCTCACCATC
GGCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1233
Start on reference sequence 148
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TOMM40
Symbol Nomenclature : TOMM40
SYNONYM : C19orf1; D19S1177E; PER-EC1; PEREC1; TOM40
Designation : mitochondrial import receptor subunit TOM40 homolog|mitochondrial outer membrane protein|p38.5|protein Haymaker|translocase of outer membrane 40 kDa subunit homolog
Full Nomenclature : translocase of outer mitochondrial membrane 40 homolog (yeast)
GENEID : 10452
HGNC : 18001
MIM : 608061
Vega : OTTHUMG00000180842
Target GenBank: BC001779

 

Reference Sequence Alignment

  

HsCD00041926        ATGGGGAACGTGTTGGCTGCCAGCTCGCCGCCCGCAGGGCCGCCACCGCCGCCTGCGCCG
NM_006114.2         ATGGGGAACGTGTTGGCTGCCAGCTCGCCGCCCGCAGGGCCGCCACCGCCGCCTGCGCCG
NM_001128916.1      ATGGGGAACGTGTTGGCTGCCAGCTCGCCGCCCGCAGGGCCGCCACCGCCGCCTGCGCCG
NM_001128917.1      ATGGGGAACGTGTTGGCTGCCAGCTCGCCGCCCGCAGGGCCGCCACCGCCGCCTGCGCCG
                    ************************************************************

HsCD00041926        GCCCTCGTGGGGCTGCCGCCACCTCCGCCCTCGCCGCCGGGCTTCACGCTGCCGCCGCTG
NM_006114.2         GCCCTCGTGGGGCTGCCGCCACCTCCGCCCTCGCCGCCGGGCTTCACGCTGCCGCCGCTG
NM_001128916.1      GCCCTCGTGGGGCTGCCGCCACCTCCGCCCTCGCCGCCGGGCTTCACGCTGCCGCCGCTG
NM_001128917.1      GCCCTCGTGGGGCTGCCGCCACCTCCGCCCTCGCCGCCGGGCTTCACGCTGCCGCCGCTG
                    ************************************************************

HsCD00041926        GGAGGCAGCCTGGGCGCCGGCACCAGTACGAGTCGAAGTTCGGAACGGACCCCCGGGGCT
NM_006114.2         GGAGGCAGCCTGGGCGCCGGCACCAGTACGAGTCGAAGTTCGGAACGGACCCCCGGGGCT
NM_001128916.1      GGAGGCAGCCTGGGCGCCGGCACCAGTACGAGTCGAAGTTCGGAACGGACCCCCGGGGCT
NM_001128917.1      GGAGGCAGCCTGGGCGCCGGCACCAGTACGAGTCGAAGTTCGGAACGGACCCCCGGGGCT
                    ************************************************************

HsCD00041926        GCAACCGCCAGCGCCTCAGGGGCCGCCGAGGATGGGGCCTGCGGCTGCCTGCCCAACCCG
NM_006114.2         GCAACCGCCAGCGCCTCAGGGGCCGCCGAGGATGGGGCCTGCGGCTGCCTGCCCAACCCG
NM_001128916.1      GCAACCGCCAGCGCCTCAGGGGCCGCCGAGGATGGGGCCTGCGGCTGCCTGCCCAACCCG
NM_001128917.1      GCAACCGCCAGCGCCTCAGGGGCCGCCGAGGATGGGGCCTGCGGCTGCCTGCCCAACCCG
                    ************************************************************

HsCD00041926        GGCACATTCGAGGAGTGCCACCGGAAGTGCAAGGAGCTGTTTCCCATTCAGATGGAGGGT
NM_006114.2         GGCACATTCGAGGAGTGCCACCGGAAGTGCAAGGAGCTGTTTCCCATTCAGATGGAGGGT
NM_001128916.1      GGCACATTCGAGGAGTGCCACCGGAAGTGCAAGGAGCTGTTTCCCATTCAGATGGAGGGT
NM_001128917.1      GGCACATTCGAGGAGTGCCACCGGAAGTGCAAGGAGCTGTTTCCCATTCAGATGGAGGGT
                    ************************************************************

HsCD00041926        GTCAAGCTCACAGTCAACAAAGGGTTGAGTAACCATTTTCAGGTCAACCACACAGTAGCC
NM_006114.2         GTCAAGCTCACAGTCAACAAAGGGTTGAGTAACCATTTTCAGGTCAACCACACAGTAGCC
NM_001128916.1      GTCAAGCTCACAGTCAACAAAGGGTTGAGTAACCATTTTCAGGTCAACCACACAGTAGCC
NM_001128917.1      GTCAAGCTCACAGTCAACAAAGGGTTGAGTAACCATTTTCAGGTCAACCACACAGTAGCC
                    ************************************************************

HsCD00041926        CTCAGCACAATCGGGGAGTCCAACTACCACTTCGGGGTCACATATGTGGGGACAAAGCAG
NM_006114.2         CTCAGCACAATCGGGGAGTCCAACTACCACTTCGGGGTCACATATGTGGGGACAAAGCAG
NM_001128916.1      CTCAGCACAATCGGGGAGTCCAACTACCACTTCGGGGTCACATATGTGGGGACAAAGCAG
NM_001128917.1      CTCAGCACAATCGGGGAGTCCAACTACCACTTCGGGGTCACATATGTGGGGACAAAGCAG
                    ************************************************************

HsCD00041926        CTGAGTCCCACAGAGGCGTTCCCTGTACTGGTGGGTGACATGGACAACAGTGGCAGTCTC
NM_006114.2         CTGAGTCCCACAGAGGCGTTCCCTGTACTGGTGGGTGACATGGACAACAGTGGCAGTCTC
NM_001128916.1      CTGAGTCCCACAGAGGCGTTCCCTGTACTGGTGGGTGACATGGACAACAGTGGCAGTCTC
NM_001128917.1      CTGAGTCCCACAGAGGCGTTCCCTGTACTGGTGGGTGACATGGACAACAGTGGCAGTCTC
                    ************************************************************

HsCD00041926        AACGCTCAGGTCATTCACCAGCTGGGCCCCGGTCTCAGGTCCAAGATGGCCATCCAGACC
NM_006114.2         AACGCTCAGGTCATTCACCAGCTGGGCCCCGGTCTCAGGTCCAAGATGGCCATCCAGACC
NM_001128916.1      AACGCTCAGGTCATTCACCAGCTGGGCCCCGGTCTCAGGTCCAAGATGGCCATCCAGACC
NM_001128917.1      AACGCTCAGGTCATTCACCAGCTGGGCCCCGGTCTCAGGTCCAAGATGGCCATCCAGACC
                    ************************************************************

HsCD00041926        CAGCAGTCGAAGTTTGTGAACTGGCAGGTGGACGGGGAGTATCGGGGCTCTGACTTCACA
NM_006114.2         CAGCAGTCGAAGTTTGTGAACTGGCAGGTGGACGGGGAGTATCGGGGCTCTGACTTCACA
NM_001128916.1      CAGCAGTCGAAGTTTGTGAACTGGCAGGTGGACGGGGAGTATCGGGGCTCTGACTTCACA
NM_001128917.1      CAGCAGTCGAAGTTTGTGAACTGGCAGGTGGACGGGGAGTATCGGGGCTCTGACTTCACA
                    ************************************************************

HsCD00041926        GCAGCCGTCACCCTGGGGAACCCAGACGTCCTCGTGGGTTCAGGAATCCTCGTAGCCCAC
NM_006114.2         GCAGCCGTCACCCTGGGGAACCCAGACGTCCTCGTGGGTTCAGGAATCCTCGTAGCCCAC
NM_001128916.1      GCAGCCGTCACCCTGGGGAACCCAGACGTCCTCGTGGGTTCAGGAATCCTCGTAGCCCAC
NM_001128917.1      GCAGCCGTCACCCTGGGGAACCCAGACGTCCTCGTGGGTTCAGGAATCCTCGTAGCCCAC
                    ************************************************************

HsCD00041926        TACCTCCAGAGCATCACGCCTTGCCTGGCCCTGGGTGGAGAGCTGGTCTACCACCGGCGG
NM_006114.2         TACCTCCAGAGCATCACGCCTTGCCTGGCCCTGGGTGGAGAGCTGGTCTACCACCGGCGG
NM_001128916.1      TACCTCCAGAGCATCACGCCTTGCCTGGCCCTGGGTGGAGAGCTGGTCTACCACCGGCGG
NM_001128917.1      TACCTCCAGAGCATCACGCCTTGCCTGGCCCTGGGTGGAGAGCTGGTCTACCACCGGCGG
                    ************************************************************

HsCD00041926        CCTGGAGAGGAGGGCACTGTCATGTCTCTAGCTGGGAAATACACATTGAACAACTGGTTG
NM_006114.2         CCTGGAGAGGAGGGCACTGTCATGTCTCTAGCTGGGAAATACACATTGAACAACTGGTTG
NM_001128916.1      CCTGGAGAGGAGGGCACTGTCATGTCTCTAGCTGGGAAATACACATTGAACAACTGGTTG
NM_001128917.1      CCTGGAGAGGAGGGCACTGTCATGTCTCTAGCTGGGAAATACACATTGAACAACTGGTTG
                    ************************************************************

HsCD00041926        GCAACGGTAACGTTGGGCCAGGCGGGCATGCACGCAACATACTACCACAAAGCCAGTGAC
NM_006114.2         GCAACGGTAACGTTGGGCCAGGCGGGCATGCACGCAACATACTACCACAAAGCCAGTGAC
NM_001128916.1      GCAACGGTAACGTTGGGCCAGGCGGGCATGCACGCAACATACTACCACAAAGCCAGTGAC
NM_001128917.1      GCAACGGTAACGTTGGGCCAGGCGGGCATGCACGCAACATACTACCACAAAGCCAGTGAC
                    ************************************************************

HsCD00041926        CAGCTGCAGGTGGGTGTGGAGTTTGAGGCCAGCACAAGGATGCAGGACACCAGCGTCTCC
NM_006114.2         CAGCTGCAGGTGGGTGTGGAGTTTGAGGCCAGCACAAGGATGCAGGACACCAGCGTCTCC
NM_001128916.1      CAGCTGCAGGTGGGTGTGGAGTTTGAGGCCAGCACAAGGATGCAGGACACCAGCGTCTCC
NM_001128917.1      CAGCTGCAGGTGGGTGTGGAGTTTGAGGCCAGCACAAGGATGCAGGACACCAGCGTCTCC
                    ************************************************************

HsCD00041926        TTCGGGTACCAGCTGGACCTGCCCAAGGCCAACCTCCTCTTCAAAGGCTCTGTGGATAGC
NM_006114.2         TTCGGGTACCAGCTGGACCTGCCCAAGGCCAACCTCCTCTTCAAAGGCTCTGTGGATAGC
NM_001128916.1      TTCGGGTACCAGCTGGACCTGCCCAAGGCCAACCTCCTCTTCAAAGGCTCTGTGGATAGC
NM_001128917.1      TTCGGGTACCAGCTGGACCTGCCCAAGGCCAACCTCCTCTTCAAAGGCTCTGTGGATAGC
                    ************************************************************

HsCD00041926        AACTGGATCGTGGGTGCCACGCTGGAGAAGAAGCTCCCACCCCTGCCCCTGACACTGGCC
NM_006114.2         AACTGGATCGTGGGTGCCACGCTGGAGAAGAAGCTCCCACCCCTGCCCCTGACACTGGCC
NM_001128916.1      AACTGGATCGTGGGTGCCACGCTGGAGAAGAAGCTCCCACCCCTGCCCCTGACACTGGCC
NM_001128917.1      AACTGGATCGTGGGTGCCACGCTGGAGAAGAAGCTCCCACCCCTGCCCCTGACACTGGCC
                    ************************************************************

HsCD00041926        CTTGGGGCCTTCCTGAATCACCGCAAGAACAAGTTTCAGTGTGGCTTTGGCCTCACCATC
NM_006114.2         CTTGGGGCCTTCCTGAATCACCGCAAGAACAAGTTTCAGTGTGGCTTTGGCCTCACCATC
NM_001128916.1      CTTGGGGCCTTCCTGAATCACCGCAAGAACAAGTTTCAGTGTGGCTTTGGCCTCACCATC
NM_001128917.1      CTTGGGGCCTTCCTGAATCACCGCAAGAACAAGTTTCAGTGTGGCTTTGGCCTCACCATC
                    ************************************************************

HsCD00041926        GGCTTG
NM_006114.2         GGCTGA
NM_001128916.1      GGCTGA
NM_001128917.1      GGCTGA
                    **** .

Pathways

Reactome : Metabolism of proteins
Reactome : Mitochondrial protein import

Protein

UniProt : O96008
HPRD : 16274

Clone

Cloning Information : 191000
HIP Master Clone ID : 165944
Original Clone ID : FLH191000.01L

Organism


TAX_ID : 9606
Species Specific ID: 10452

Genome

Chromosome : 19
Map Location : 19q13
Ensembl : ENSG00000130204

Reagents

Labome : TOMM40-antibody