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TCEB3 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  TCEB3
Gene Name:  transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH191794.01L
Keyword: nan
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00042009

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2316nts        
Open reading frame : 1 to 2316
Reference Sequence Alignment

ATGGCGGCGGAGTCGGCGCTCCAAGTTGTGGAGAAGCTGCAGGCGCGCCTGGCCGCGAAC
CCGGACCCTAAGAAGCTATTGAAATATTTGAAGAAACTCTCCACCCTGCCTATTACAGTA
GACATTCTTGCGGAGACTGGGGTTGGGAAAACAGTAAATAGCTTGCGAAAACACGAGCAT
GTTGGAAGCTTTGCCAGGGACCTAGTGGCCCAGTGGAAGAAGCTGGTTCCTGTGGAACGA
AATGCTGAGCCTGATGAACAGGACTTTGAGAAGAGCAATTCCCGAAAGCGCCCTCGGGAT
GCCCTGCAGAAGGAGGAGGAGATGGAGGGGGACTACCAAGAAACCTGGAAAGCCACGGGG
AGCCGATCCTATAGCCCTGACCACAGGCAGAAGAAACATAGGAAACTCTCGGAGCTCGAG
AGACCTCACAAAGTGTCTCACGGTCATGAGAGGAGAGATGAGAGAAAGAGGTGTCACAGA
ATGTCACCAACTTACTCTTCAGACCCTGAGTCTTCTGATTATGGCCATGTTCAATCCCCT
CCATCTTGTACCAGTCCTCATCAGATGTACGTCGACCACTACAGATCCCTGGAGGAGGAC
CAGGAGCCCATTGTTTCACACCAGAAGCCTGGGAAAGGCCACAGCAATGCCTTTCAGGAC
AGACTCGGGGCCAGCCAAGAACGACACCTGGGTGAACCCCATGGGAAAGGGGTTGTGAGT
CAAAACAAGGAGCACAAATCTTCCCACAAGGACAAACGCCCCGTGGATGCCAAGAGTGAT
GAGAAGGCCTCTGTGGTGAGCAGAGAGAAATCACACAAGGCCCTCTCCAAAGAGGAGAAC
CGAAGGCCACCCTCAGGGGACAATGCAAGGGAGAAACCGCCCTCTAGTGGCGTAAAGAAA
GAGAAGGACAGAGAGGGCAGCAGCCTGAAGAAGAAGTGTTTGCCTCCCTCAGAGGCCGCT
TCAGACAACCACCTGAAAAAGCCAAAGCACAGAGACCCAGAGAAAGCCAAATTGGACAAA
AGCAAGCAAGGTCTGGACAGCTTTGACACAGGAAAAGGAGCAGGAGACCTGTTGCCCAAG
GTAAAAGAGAAGGGTTCTAACAACCTAAAGACTCCAGAAGGGAAAGTCAAAACTAATTTG
GATAGAAAGTCACTGGGCTCCCTCCCTAAAGTTGAGGAGACAGATATGGAGGATGAATTC
GAGCAGCCAACCATGTCTTTTGAATCCTACCTCAGCTATGACCAGCCCCGGAAGAAAAAG
AAAAAGATTGTGAAAACTTCAGCCACGGCACTTGGAGATAAAGGACTTAAAAAAAATGAC
TCTAAAAGCACTGGTAAAAACTTGGACTCAGTTCAGAAATTACCCAAGGTGAACAAAACC
AAGTCAGAGAAGCCGGCTGGAGCTGATTTAGCCAAGCTGAGAAAGGTGCCTGATGTGTTG
CCAGTGTTGCCAGACCTCCCGTTACCCGCGATACAGGCCAATTACCGTCCACTGCCTTCC
CTCGAGCTGATATCCTCCTTCCAGCCAAAGCGAAAAGCGTTCTCTTCACCCCAGGAAGAA
GAAGAAGCTGGATTTACTGGGCGCAGAATGAATTCCAAGATGCAGGTGTATTCTGGTTCC
AAGTGTGCCTATCTCCCTAAAATGATGACCTTGCACCAGCAATGCATCCGAGTACTTAAA
AACAACATCGATTCAATCTTTGAAGTGGGAGGAGTCCCATACTCTGTTCTTGAACCCGTT
TTGGAGAGGTGTACACCTGATCAGCTGTATCGCATAGAGGAATACAATCATGTATTAATT
GAAGAAACAGATCAATTATGGAAAGTTCATTGTCACCGAGACTTTAAGGAAGAAAGACCC
GAAGAGTATGAGTCGTGGCGAGAGATGTACCTGCGGCTTCAGGACGCCCGAGAGCAGCGG
CTACGAGTACTAACAAAGAATATCCAGTTCGCACATGCCAATAAGCCCAAAGGCCGACAA
GCAAAGATGGCCTTTGTCAACTCTGTGGCCAAGCCACCTCGTGACGTCCGGAGGAGGCAG
GAAAAGTTTGGAACGGGAGGAGCAGCTGTCCCTGAGAAAATCAAGATCAAGCCAGCCCCG
TACCCCATGGGAAGCAGCCATGCTTCCGCCAGTAGTATCAGCTTTAACCCCAGCCCTGAG
GAGCCGGCCTATGATGGCCCAAGCACCAGCAGTGCCCACTTGGCACCAGTGGTCAGCAGC
ACTGTTTCCTATGATCCTAGGAAACCCACTGTGAAGAAAATTGCCCCAATGATGGCCAAG
ACAATTAAAGCTTTCAAGAACAGATTCTCCCGACGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2350
Start on reference sequence 32
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site U19 primer 517 535
primer site Att P2 Primer 3026 3043
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
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  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TCEB3
Symbol Nomenclature : TCEB3
SYNONYM : EloA; SIII; SIII p110; TCEB3A
Designation : RNA polymerase II transcription factor SIII subunit A1|elongin 110 kDa subunit|elongin-A|transcription elongation factor B alpha subunit|transcription elongation factor B polypeptide 3
Full Nomenclature : transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)
GENEID : 6924
Locus Tag : MSTP059
HGNC : 11620
MIM : 600786
Vega : OTTHUMG00000002957
Target GenBank: BC002883

 

Reference Sequence Alignment

  

HsCD00042009      ------------------------------------------------------------
NM_003198.2       ATGCACGGAGGGCGGAGCTGCGGCCCGAGGACGCGACGCGAGCCCAGTTCCGGCGAGGAG
                                                                              

HsCD00042009      ------------------ATGGCGGCGGAGTCGGCGCTCCAAGTTGTGGAGAAGCTGCAG
NM_003198.2       GCCGCGCCAGTGACAGCGATGGCGGCGGAGTCGGCGCTCCAAGTTGTGGAGAAGCTGCAG
                                    ******************************************

HsCD00042009      GCGCGCCTGGCCGCGAACCCGGACCCTAAGAAGCTATTGAAATATTTGAAGAAACTCTCC
NM_003198.2       GCGCGCCTGGCCGCGAACCCGGACCCTAAGAAGCTATTGAAATATTTGAAGAAACTCTCC
                  ************************************************************

HsCD00042009      ACCCTGCCTATTACAGTAGACATTCTTGCGGAGACTGGGGTTGGGAAAACAGTAAATAGC
NM_003198.2       ACCCTGCCTATTACAGTAGACATTCTTGCGGAGACTGGGGTTGGGAAAACAGTAAATAGC
                  ************************************************************

HsCD00042009      TTGCGAAAACACGAGCATGTTGGAAGCTTTGCCAGGGACCTAGTGGCCCAGTGGAAGAAG
NM_003198.2       TTGCGAAAACACGAGCATGTTGGAAGCTTTGCCAGGGACCTAGTGGCCCAGTGGAAGAAG
                  ************************************************************

HsCD00042009      CTGGTTCCTGTGGAACGAAATGCTGAGCCTGATGAACAGGACTTTGAGAAGAGCAATTCC
NM_003198.2       CTGGTTCCTGTGGAACGAAATGCTGAGCCTGATGAACAGGACTTTGAGAAGAGCAATTCC
                  ************************************************************

HsCD00042009      CGAAAGCGCCCTCGGGATGCCCTGCAGAAGGAGGAGGAGATGGAGGGGGACTACCAAGAA
NM_003198.2       CGAAAGCGCCCTCGGGATGCCCTGCAGAAGGAGGAGGAGATGGAGGGGGACTACCAAGAA
                  ************************************************************

HsCD00042009      ACCTGGAAAGCCACGGGGAGCCGATCCTATAGCCCTGACCACAGGCAGAAGAAACATAGG
NM_003198.2       ACCTGGAAAGCCACGGGGAGCCGATCCTATAGCCCTGACCACAGGCAGAAGAAACATAGG
                  ************************************************************

HsCD00042009      AAACTCTCGGAGCTCGAGAGACCTCACAAAGTGTCTCACGGTCATGAGAGGAGAGATGAG
NM_003198.2       AAACTCTCGGAGCTCGAGAGACCTCACAAAGTGTCTCACGGTCATGAGAGGAGAGATGAG
                  ************************************************************

HsCD00042009      AGAAAGAGGTGTCACAGAATGTCACCAACTTACTCTTCAGACCCTGAGTCTTCTGATTAT
NM_003198.2       AGAAAGAGGTGTCACAGAATGTCACCAACTTACTCTTCAGACCCTGAGTCTTCTGATTAT
                  ************************************************************

HsCD00042009      GGCCATGTTCAATCCCCTCCATCTTGTACCAGTCCTCATCAGATGTACGTCGACCACTAC
NM_003198.2       GGCCATGTTCAATCCCCTCCATCTTGTACCAGTCCTCATCAGATGTACGTCGACCACTAC
                  ************************************************************

HsCD00042009      AGATCCCTGGAGGAGGACCAGGAGCCCATTGTTTCACACCAGAAGCCTGGGAAAGGCCAC
NM_003198.2       AGATCCCTGGAGGAGGACCAGGAGCCCATTGTTTCACACCAGAAGCCTGGGAAAGGCCAC
                  ************************************************************

HsCD00042009      AGCAATGCCTTTCAGGACAGACTCGGGGCCAGCCAAGAACGACACCTGGGTGAACCCCAT
NM_003198.2       AGCAATGCCTTTCAGGACAGACTCGGGGCCAGCCAAGAACGACACCTGGGTGAACCCCAT
                  ************************************************************

HsCD00042009      GGGAAAGGGGTTGTGAGTCAAAACAAGGAGCACAAATCTTCCCACAAGGACAAACGCCCC
NM_003198.2       GGGAAAGGGGTTGTGAGTCAAAACAAGGAGCACAAATCTTCCCACAAGGACAAACGCCCC
                  ************************************************************

HsCD00042009      GTGGATGCCAAGAGTGATGAGAAGGCCTCTGTGGTGAGCAGAGAGAAATCACACAAGGCC
NM_003198.2       GTGGATGCCAAGAGTGATGAGAAGGCCTCTGTGGTGAGCAGAGAGAAATCACACAAGGCC
                  ************************************************************

HsCD00042009      CTCTCCAAAGAGGAGAACCGAAGGCCACCCTCAGGGGACAATGCAAGGGAGAAACCGCCC
NM_003198.2       CTCTCCAAAGAGGAGAACCGAAGGCCACCCTCAGGGGACAATGCAAGGGAGAAACCGCCC
                  ************************************************************

HsCD00042009      TCTAGTGGCGTAAAGAAAGAGAAGGACAGAGAGGGCAGCAGCCTGAAGAAGAAGTGTTTG
NM_003198.2       TCTAGTGGCGTAAAGAAAGAGAAGGACAGAGAGGGCAGCAGCCTGAAGAAGAAGTGTTTG
                  ************************************************************

HsCD00042009      CCTCCCTCAGAGGCCGCTTCAGACAACCACCTGAAAAAGCCAAAGCACAGAGACCCAGAG
NM_003198.2       CCTCCCTCAGAGGCCGCTTCAGACAACCACCTGAAAAAGCCAAAGCACAGAGACCCAGAG
                  ************************************************************

HsCD00042009      AAAGCCAAATTGGACAAAAGCAAGCAAGGTCTGGACAGCTTTGACACAGGAAAAGGAGCA
NM_003198.2       AAAGCCAAATTGGACAAAAGCAAGCAAGGTCTGGACAGCTTTGACACAGGAAAAGGAGCA
                  ************************************************************

HsCD00042009      GGAGACCTGTTGCCCAAGGTAAAAGAGAAGGGTTCTAACAACCTAAAGACTCCAGAAGGG
NM_003198.2       GGAGACCTGTTGCCCAAGGTAAAAGAGAAGGGTTCTAACAACCTAAAGACTCCAGAAGGG
                  ************************************************************

HsCD00042009      AAAGTCAAAACTAATTTGGATAGAAAGTCACTGGGCTCCCTCCCTAAAGTTGAGGAGACA
NM_003198.2       AAAGTCAAAACTAATTTGGATAGAAAGTCACTGGGCTCCCTCCCTAAAGTTGAGGAGACA
                  ************************************************************

HsCD00042009      GATATGGAGGATGAATTCGAGCAGCCAACCATGTCTTTTGAATCCTACCTCAGCTATGAC
NM_003198.2       GATATGGAGGATGAATTCGAGCAGCCAACCATGTCTTTTGAATCCTACCTCAGCTATGAC
                  ************************************************************

HsCD00042009      CAGCCCCGGAAGAAAAAGAAAAAGATTGTGAAAACTTCAGCCACGGCACTTGGAGATAAA
NM_003198.2       CAGCCCCGGAAGAAAAAGAAAAAGATTGTGAAAACTTCAGCCACGGCACTTGGAGATAAA
                  ************************************************************

HsCD00042009      GGACTTAAAAAAAATGACTCTAAAAGCACTGGTAAAAACTTGGACTCAGTTCAGAAATTA
NM_003198.2       GGACTTAAAAAAAATGACTCTAAAAGCACTGGTAAAAACTTGGACTCAGTTCAGAAATTA
                  ************************************************************

HsCD00042009      CCCAAGGTGAACAAAACCAAGTCAGAGAAGCCGGCTGGAGCTGATTTAGCCAAGCTGAGA
NM_003198.2       CCCAAGGTGAACAAAACCAAGTCAGAGAAGCCGGCTGGAGCTGATTTAGCCAAGCTGAGA
                  ************************************************************

HsCD00042009      AAGGTGCCTGATGTGTTGCCAGTGTTGCCAGACCTCCCGTTACCCGCGATACAGGCCAAT
NM_003198.2       AAGGTGCCTGATGTGTTGCCAGTGTTGCCAGACCTCCCGTTACCCGCGATACAGGCCAAT
                  ************************************************************

HsCD00042009      TACCGTCCACTGCCTTCCCTCGAGCTGATATCCTCCTTCCAGCCAAAGCGAAAAGCGTTC
NM_003198.2       TACCGTCCACTGCCTTCCCTCGAGCTGATATCCTCCTTCCAGCCAAAGCGAAAAGCGTTC
                  ************************************************************

HsCD00042009      TCTTCACCCCAGGAAGAAGAAGAAGCTGGATTTACTGGGCGCAGAATGAATTCCAAGATG
NM_003198.2       TCTTCACCCCAGGAAGAAGAAGAAGCTGGATTTACTGGGCGCAGAATGAATTCCAAGATG
                  ************************************************************

HsCD00042009      CAGGTGTATTCTGGTTCCAAGTGTGCCTATCTCCCTAAAATGATGACCTTGCACCAGCAA
NM_003198.2       CAGGTGTATTCTGGTTCCAAGTGTGCCTATCTCCCTAAAATGATGACCTTGCACCAGCAA
                  ************************************************************

HsCD00042009      TGCATCCGAGTACTTAAAAACAACATCGATTCAATCTTTGAAGTGGGAGGAGTCCCATAC
NM_003198.2       TGCATCCGAGTACTTAAAAACAACATCGATTCAATCTTTGAAGTGGGAGGAGTCCCATAC
                  ************************************************************

HsCD00042009      TCTGTTCTTGAACCCGTTTTGGAGAGGTGTACACCTGATCAGCTGTATCGCATAGAGGAA
NM_003198.2       TCTGTTCTTGAACCCGTTTTGGAGAGGTGTACACCTGATCAGCTGTATCGCATAGAGGAA
                  ************************************************************

HsCD00042009      TACAATCATGTATTAATTGAAGAAACAGATCAATTATGGAAAGTTCATTGTCACCGAGAC
NM_003198.2       TACAATCATGTATTAATTGAAGAAACAGATCAATTATGGAAAGTTCATTGTCACCGAGAC
                  ************************************************************

HsCD00042009      TTTAAGGAAGAAAGACCCGAAGAGTATGAGTCGTGGCGAGAGATGTACCTGCGGCTTCAG
NM_003198.2       TTTAAGGAAGAAAGACCCGAAGAGTATGAGTCGTGGCGAGAGATGTACCTGCGGCTTCAG
                  ************************************************************

HsCD00042009      GACGCCCGAGAGCAGCGGCTACGAGTACTAACAAAGAATATCCAGTTCGCACATGCCAAT
NM_003198.2       GACGCCCGAGAGCAGCGGCTACGAGTACTAACAAAGAATATCCAGTTCGCACATGCCAAT
                  ************************************************************

HsCD00042009      AAGCCCAAAGGCCGACAAGCAAAGATGGCCTTTGTCAACTCTGTGGCCAAGCCACCTCGT
NM_003198.2       AAGCCCAAAGGCCGACAAGCAAAGATGGCCTTTGTCAACTCTGTGGCCAAGCCACCTCGT
                  ************************************************************

HsCD00042009      GACGTCCGGAGGAGGCAGGAAAAGTTTGGAACGGGAGGAGCAGCTGTCCCTGAGAAAATC
NM_003198.2       GACGTCCGGAGGAGGCAGGAAAAGTTTGGAACGGGAGGAGCAGCTGTCCCTGAGAAAATC
                  ************************************************************

HsCD00042009      AAGATCAAGCCAGCCCCGTACCCCATGGGAAGCAGCCATGCTTCCGCCAGTAGTATCAGC
NM_003198.2       AAGATCAAGCCAGCCCCGTACCCCATGGGAAGCAGCCATGCTTCCGCCAGTAGCATCAGC
                  ***************************************************** ******

HsCD00042009      TTTAACCCCAGCCCTGAGGAGCCGGCCTATGATGGCCCAAGCACCAGCAGTGCCCACTTG
NM_003198.2       TTTAACCCCAGCCCTGAGGAGCCGGCCTATGATGGCCCAAGCACCAGCAGTGCCCACTTG
                  ************************************************************

HsCD00042009      GCACCAGTGGTCAGCAGCACTGTTTCCTATGATCCTAGGAAACCCACTGTGAAGAAAATT
NM_003198.2       GCACCAGTGGTCAGCAGCACTGTTTCCTATGATCCTAGGAAACCCACTGTGAAGAAAATT
                  ************************************************************

HsCD00042009      GCCCCAATGATGGCCAAGACAATTAAAGCTTTCAAGAACAGATTCTCCCGACGATTG
NM_003198.2       GCCCCAATGATGGCCAAGACAATTAAAGCTTTCAAGAACAGATTCTCCCGACGATAA
                  *******************************************************:.

Pathways

Reactome : Disease
Reactome : Elongation arrest and recovery
Reactome : Formation of HIV elongation complex in the absence of HIV Tat
Reactome : Formation of HIV-1 elongation complex containing HIV-1 Tat
Reactome : Formation of RNA Pol II elongation complex
Reactome : Gene Expression
Reactome : HIV elongation arrest and recovery
Reactome : HIV Infection
Reactome : HIV Life Cycle
Reactome : HIV Transcription Elongation
Reactome : Late Phase of HIV Life Cycle
Reactome : Pausing and recovery of HIV elongation
Reactome : Pausing and recovery of Tat-mediated HIV elongation
Reactome : RNA Polymerase II Pre-transcription Events
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Elongation
Reactome : Tat-mediated elongation of the HIV-1 transcript
Reactome : Tat-mediated HIV elongation arrest and recovery
Reactome : Transcription
Reactome : Transcription of the HIV genome

Clone

Cloning Information : 191794
HIP Master Clone ID : 166738
Original Clone ID : FLH191794.01L

Organism


TAX_ID : 9606
Species Specific ID: 6924

Genome

Chromosome : 1
Map Location : 1p36.1
Ensembl : ENSG00000011007

Reagents

Labome : TCEB3-antibody