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CCT7 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CCT7
Gene Name:  chaperonin containing TCP1, subunit 7 (eta)
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH193370.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00042046

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1632nts         Open reading frame : 1 to 1632
Reference Sequence Alignment

ATGATGCCCACACCAGTTATCCTATTGAAAGAGGGGACTGATAGCTCCCAAGGCATCCCC
CAGCTTGTGAGTAACATCAGTGCCTGCCAGGTGATTGCTGAGGCTGTAAGAACTACCCTG
GGTCCCCGTGGCATGGACAAGCTTATTGTAGATGGCAGAGGCAAAGCAACAATTTCTAAT
GATGGGGCCACAATTCTGAAACTTCTTGATGTTGTCCATCCTGCAGCAAAGACTTTGGTA
GACATTGCCAAATCCCAAGATGCTGAGGTGGGTGATGGCACCACCTCAGTGACCTTGCTG
GCTGCAGAGTTTCTGAAGCAGGTGAAACCCTATGTGGAGGAAGGTTTACACCCCCAGATC
ATCATTCGAGCTTTCCGCACAGCCACCCAGCTGGCAGTTAACAAGATCAAAGAGATTGCT
GTGACCGTGAAGAAGGCAGATAAAGTGGAGCAGAGGAAGCTGCTGGAAAAGTGTGCCATG
ACCGCTCTGAGCTCCAAGCTGATCTCCCAGCAGAAAGCTTTCTTTGCTAAGATGGTGGTG
GATGCAGTGATGATGCTCGATGATTTGCTGCAGCTTAAAATGATTGGAATCAAGAAGGTA
CAGGGTGGAGCCCTCGAGGATTCTCAGCTGGTAGCTGGTGTTGCATTCAAGAAGACTTTC
TCTTACGCTGGGTTTGAAATGCAACCCAAAAAGTACCACAATCCCAAGATTGCCCTTTTG
AATGTCGAGCTCGAGTTGAAAGCTGAGAAAGACAATGCTGAGATAAGAGTCCACACAGTT
GAGGATTATCAGGCAATTGTTGATGCTGAGTGGAACATTCTCTATGACAAGTTAGAGAAG
ATCCATCATTCTGGAGCCAAAGTTGTCTTGTCCAAACTCCCCATTGGGGATGTGGCCACC
CAGTACTTTGCTGACAGGGACATGTTCTGTGCTGGCCGAGTACCTGAGGAGGATCTGAAG
AGGACAATGATGGCCTGTGGAGGCTCAATCCAGACCAGTGTGAATGCTCTGTCAGCAGAT
GTGCTGGGTCGATGCCAGGTGTTTGAAGAGACCCAGATTGGAGGCGAGAGGTACAATTTT
TTTACTGGCTGCCCCAAGGCCAAGACATGCACCTTCATTCTCCGTGGCGGCGCCGAGCAG
TTTATGGAGGAGACAGAGCGGTCCCTGCATGATGCCATCATGATCGTCAGGAGGGCCATC
AAGAATGATTCAGTGGTGGCTGGTGGCGGGGCCATTGAGATGGAACTCTCCAAGTACCTG
CGGGATTACTCAAGGACTATTCCAGGAAAACAGCAGCTGTTGATTGGGGCTTATGCCAAG
GCCTTGGAGATTATCCCACGCCAGCTGTGTGACAATGCTGGCTTTGATGCCACAAACATT
CTCAACAAGCTGCGGGCTCGGCATGCCCAGGGGGGTACATGGTATGGAGTAGACATCAAC
AACGAGGACATTGCTGACAACTTTGAAGCTTTCGTGTGGGAGCCAGCTATGGTGCGGATC
AATGCGCTGACAGCAGCCTCTGAGGCTGCGTGCCTGATCGTGTCTGTAGATGAAACCATC
AAGAACCCCCGCTCGACTGTGGATGCTCCCACAGCAGCAGGCCGGGGCCGTGGTCGTGGC
CGCCCCCACTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1688
Start on reference sequence 57
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
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  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CCT7
Symbol Nomenclature : CCT7
SYNONYM : CCTETA; CCTH; NIP7-1; TCP1ETA
Designation : CCT-eta|HIV-1 Nef interacting protein|HIV-1 Nef-interacting protein|T-complex protein 1 subunit eta|TCP-1-eta|chaperonin containing t-complex polypeptide 1, eta subunit
Full Nomenclature : chaperonin containing TCP1, subunit 7 (eta)
GENEID : 10574
HGNC : 1622
MIM : 605140
Vega : OTTHUMG00000152765
Target GenBank: BC019296

 

Reference Sequence Alignment

  

HsCD00042046        ATGATGCCCACACCAGTTATCCTATTGAAAGAGGGGACTGATAGCTCCCAAGGCATCCCC
NM_006429.3         ATGATGCCCACACCAGTTATCCTATTGAAAGAGGGGACTGATAGCTCCCAAGGCATCCCC
NM_001166285.1      ------------------------------------------------------------
                                                                                

HsCD00042046        CAGCTTGTGAGTAACATCAGTGCCTGCCAGGTGATTGCTGAGGCTGTAAGAACTACCCTG
NM_006429.3         CAGCTTGTGAGTAACATCAGTGCCTGCCAGGTGATTGCTGAGGCTGTAAGAACTACCCTG
NM_001166285.1      ------------------------------------------------------------
                                                                                

HsCD00042046        GGTCCCCGTGGCATGGACAAGCTTATTGTAGATGGCAGAGGCAAAGCAACAATTTCTAAT
NM_006429.3         GGTCCCCGTGGCATGGACAAGCTTATTGTAGATGGCAGAGGCAAAGCAACAATTTCTAAT
NM_001166285.1      ------------ATGGACAAGCTTATTGTAGATGGCAGAGGCAAAGCAACAATTTCTAAT
                                ************************************************

HsCD00042046        GATGGGGCCACAATTCTGAAACTTCTTGATGTTGTCCATCCTGCAGCAAAGACTTTGGTA
NM_006429.3         GATGGGGCCACAATTCTGAAACTTCTTGATGTTGTCCATCCTGCAGCAAAGACTTTGGTA
NM_001166285.1      GATGGGGCCACAATTCTGAAACTTCTTGATGTTGTCCATCCTGCAGCAAAGACTTTGGTA
                    ************************************************************

HsCD00042046        GACATTGCCAAATCCCAAGATGCTGAGGTGGGTGATGGCACCACCTCAGTGACCTTGCTG
NM_006429.3         GACATTGCCAAATCCCAAGATGCTGAGGTGGGTGATGGCACCACCTCAGTGACCTTGCTG
NM_001166285.1      GACATTGCCAAATCCCAAGATGCTGAGGTGGGTGATGGCACCACCTCAGTGACCTTGCTG
                    ************************************************************

HsCD00042046        GCTGCAGAGTTTCTGAAGCAGGTGAAACCCTATGTGGAGGAAGGTTTACACCCCCAGATC
NM_006429.3         GCTGCAGAGTTTCTGAAGCAGGTGAAACCCTATGTGGAGGAAGGTTTACACCCCCAGATC
NM_001166285.1      GCTGCAGAGTTTCTGAAGCAGGTGAAACCCTATGTGGAGGAAGGTTTACACCCCCAGATC
                    ************************************************************

HsCD00042046        ATCATTCGAGCTTTCCGCACAGCCACCCAGCTGGCAGTTAACAAGATCAAAGAGATTGCT
NM_006429.3         ATCATTCGAGCTTTCCGCACAGCCACCCAGCTGGCAGTTAACAAGATCAAAGAGATTGCT
NM_001166285.1      ATCATTCGAGCTTTCCGCACAGCCACCCAGCTGGCAGTTAACAAGATCAAAGAGATTGCT
                    ************************************************************

HsCD00042046        GTGACCGTGAAGAAGGCAGATAAAGTGGAGCAGAGGAAGCTGCTGGAAAAGTGTGCCATG
NM_006429.3         GTGACCGTGAAGAAGGCAGATAAAGTGGAGCAGAGGAAGCTGCTGGAAAAGTGTGCCATG
NM_001166285.1      GTGACCGTGAAGAAGGCAGATAAAGTGGAGCAGAGGAAGCTGCTGGAAAAGTGTGCCATG
                    ************************************************************

HsCD00042046        ACCGCTCTGAGCTCCAAGCTGATCTCCCAGCAGAAAGCTTTCTTTGCTAAGATGGTGGTG
NM_006429.3         ACCGCTCTGAGCTCCAAGCTGATCTCCCAGCAGAAAGCTTTCTTTGCTAAGATGGTGGTG
NM_001166285.1      ACCGCTCTGAGCTCCAAGCTGATCTCCCAGCAGAAAGCTTTCTTTGCTAAGATGGTGGTG
                    ************************************************************

HsCD00042046        GATGCAGTGATGATGCTCGATGATTTGCTGCAGCTTAAAATGATTGGAATCAAGAAGGTA
NM_006429.3         GATGCAGTGATGATGCTCGATGATTTGCTGCAGCTTAAAATGATTGGAATCAAGAAGGTA
NM_001166285.1      GATGCAGTGATGATGCTCGATGATTTGCTGCAGCTTAAAATGATTGGAATCAAGAAGGTA
                    ************************************************************

HsCD00042046        CAGGGTGGAGCCCTCGAGGATTCTCAGCTGGTAGCTGGTGTTGCATTCAAGAAGACTTTC
NM_006429.3         CAGGGTGGAGCCCTCGAGGATTCTCAGCTGGTAGCTGGTGTTGCATTCAAGAAGACTTTC
NM_001166285.1      CAGGGTGGAGCCCTCGAGGATTCTCAGCTGGTAGCTGGTGTTGCATTCAAGAAGACTTTC
                    ************************************************************

HsCD00042046        TCTTACGCTGGGTTTGAAATGCAACCCAAAAAGTACCACAATCCCAAGATTGCCCTTTTG
NM_006429.3         TCTTACGCTGGGTTTGAAATGCAACCCAAAAAGTACCACAATCCCAAGATTGCCCTTTTG
NM_001166285.1      TCTTACGCTGGGTTTGAAATGCAACCCAAAAAGTACCACAATCCCAAGATTGCCCTTTTG
                    ************************************************************

HsCD00042046        AATGTCGAGCTCGAGTTGAAAGCTGAGAAAGACAATGCTGAGATAAGAGTCCACACAGTT
NM_006429.3         AATGTCGAGCTCGAGTTGAAAGCTGAGAAAGACAATGCTGAGATAAGAGTCCACACAGTT
NM_001166285.1      AATGTCGAGCTCGAGTTGAAAGCTGAGAAAGACAATGCTGAGATAAGAGTCCACACAGTT
                    ************************************************************

HsCD00042046        GAGGATTATCAGGCAATTGTTGATGCTGAGTGGAACATTCTCTATGACAAGTTAGAGAAG
NM_006429.3         GAGGATTATCAGGCAATTGTTGATGCTGAGTGGAACATTCTCTATGACAAGTTAGAGAAG
NM_001166285.1      GAGGATTATCAGGCAATTGTTGATGCTGAGTGGAACATTCTCTATGACAAGTTAGAGAAG
                    ************************************************************

HsCD00042046        ATCCATCATTCTGGAGCCAAAGTTGTCTTGTCCAAACTCCCCATTGGGGATGTGGCCACC
NM_006429.3         ATCCATCATTCTGGAGCCAAAGTTGTCTTGTCCAAACTCCCCATTGGGGATGTGGCCACC
NM_001166285.1      ATCCATCATTCTGGAGCCAAAGTTGTCTTGTCCAAACTCCCCATTGGGGATGTGGCCACC
                    ************************************************************

HsCD00042046        CAGTACTTTGCTGACAGGGACATGTTCTGTGCTGGCCGAGTACCTGAGGAGGATCTGAAG
NM_006429.3         CAGTACTTTGCTGACAGGGACATGTTCTGTGCTGGCCGAGTACCTGAGGAGGATCTGAAG
NM_001166285.1      CAGTACTTTGCTGACAGGGACATGTTCTGTGCTGGCCGAGTACCTGAGGAGGATCTGAAG
                    ************************************************************

HsCD00042046        AGGACAATGATGGCCTGTGGAGGCTCAATCCAGACCAGTGTGAATGCTCTGTCAGCAGAT
NM_006429.3         AGGACAATGATGGCCTGTGGAGGCTCAATCCAGACCAGTGTGAATGCTCTGTCAGCAGAT
NM_001166285.1      AGGACAATGATGGCCTGTGGAGGCTCAATCCAGACCAGTGTGAATGCTCTGTCAGCAGAT
                    ************************************************************

HsCD00042046        GTGCTGGGTCGATGCCAGGTGTTTGAAGAGACCCAGATTGGAGGCGAGAGGTACAATTTT
NM_006429.3         GTGCTGGGTCGATGCCAGGTGTTTGAAGAGACCCAGATTGGAGGCGAGAGGTACAATTTT
NM_001166285.1      GTGCTGGGTCGATGCCAGGTGTTTGAAGAGACCCAGATTGGAGGCGAGAGGTACAATTTT
                    ************************************************************

HsCD00042046        TTTACTGGCTGCCCCAAGGCCAAGACATGCACCTTCATTCTCCGTGGCGGCGCCGAGCAG
NM_006429.3         TTTACTGGCTGCCCCAAGGCCAAGACATGCACCTTCATTCTCCGTGGCGGCGCCGAGCAG
NM_001166285.1      TTTACTGGCTGCCCCAAGGCCAAGACATGCACCTTCATTCTCCGTGGCGGCGCCGAGCAG
                    ************************************************************

HsCD00042046        TTTATGGAGGAGACAGAGCGGTCCCTGCATGATGCCATCATGATCGTCAGGAGGGCCATC
NM_006429.3         TTTATGGAGGAGACAGAGCGGTCCCTGCATGATGCCATCATGATCGTCAGGAGGGCCATC
NM_001166285.1      TTTATGGAGGAGACAGAGCGGTCCCTGCATGATGCCATCATGATCGTCAGGAGGGCCATC
                    ************************************************************

HsCD00042046        AAGAATGATTCAGTGGTGGCTGGTGGCGGGGCCATTGAGATGGAACTCTCCAAGTACCTG
NM_006429.3         AAGAATGATTCAGTGGTGGCTGGTGGCGGGGCCATTGAGATGGAACTCTCCAAGTACCTG
NM_001166285.1      AAGAATGATTCAGTGGTGGCTGGTGGCGGGGCCATTGAGATGGAACTCTCCAAGTACCTG
                    ************************************************************

HsCD00042046        CGGGATTACTCAAGGACTATTCCAGGAAAACAGCAGCTGTTGATTGGGGCTTATGCCAAG
NM_006429.3         CGGGATTACTCAAGGACTATTCCAGGAAAACAGCAGCTGTTGATTGGGGCATATGCCAAG
NM_001166285.1      CGGGATTACTCAAGGACTATTCCAGGAAAACAGCAGCTGTTGATTGGGGCATATGCCAAG
                    **************************************************:*********

HsCD00042046        GCCTTGGAGATTATCCCACGCCAGCTGTGTGACAATGCTGGCTTTGATGCCACAAACATT
NM_006429.3         GCCTTGGAGATTATCCCACGCCAGCTGTGTGACAATGCTGGCTTTGATGCCACAAACATT
NM_001166285.1      GCCTTGGAGATTATCCCACGCCAGCTGTGTGACAATGCTGGCTTTGATGCCACAAACATT
                    ************************************************************

HsCD00042046        CTCAACAAGCTGCGGGCTCGGCATGCCCAGGGGGGTACATGGTATGGAGTAGACATCAAC
NM_006429.3         CTCAACAAGCTGCGGGCTCGGCATGCCCAGGGGGGTACATGGTATGGAGTAGACATCAAC
NM_001166285.1      CTCAACAAGCTGCGGGCTCGGCATGCCCAGGGGGGTACATGGTATGGAGTAGACATCAAC
                    ************************************************************

HsCD00042046        AACGAGGACATTGCTGACAACTTTGAAGCTTTCGTGTGGGAGCCAGCTATGGTGCGGATC
NM_006429.3         AACGAGGACATTGCTGACAACTTTGAAGCTTTCGTGTGGGAGCCAGCTATGGTGCGGATC
NM_001166285.1      AACGAGGACATTGCTGACAACTTTGAAGCTTTCGTGTGGGAGCCAGCTATGGTGCGGATC
                    ************************************************************

HsCD00042046        AATGCGCTGACAGCAGCCTCTGAGGCTGCGTGCCTGATCGTGTCTGTAGATGAAACCATC
NM_006429.3         AATGCGCTGACAGCAGCCTCTGAGGCTGCGTGCCTGATCGTGTCTGTAGATGAAACCATC
NM_001166285.1      AATGCGCTGACAGCAGCCTCTGAGGCTGCGTGCCTGATCGTGTCTGTAGATGAAACCATC
                    ************************************************************

HsCD00042046        AAGAACCCCCGCTCGACTGTGGATGCTCCCACAGCAGCAGGCCGGGGCCGTGGTCGTGGC
NM_006429.3         AAGAACCCCCGCTCGACTGTGGATGCTCCCACAGCAGCAGGCCGGGGCCGTGGTCGTGGC
NM_001166285.1      AAGAACCCCCGCTCGACTGTGGATGCTCCCACAGCAGCAGGCCGGGGCCGTGGTCGTGGC
                    ************************************************************

HsCD00042046        CGCCCCCACTTG
NM_006429.3         CGCCCCCACTGA
NM_001166285.1      CGCCCCCACTGA
                    ********** .

Pathways

Reactome : Association of TriC/CCT with target proteins during biosynthesis
Reactome : Chaperonin-mediated protein folding
Reactome : Cooperation of Prefoldin and TriC/CCT in actin and tubulin folding
Reactome : Folding of actin by CCT/TriC
Reactome : Formation of tubulin folding intermediates by CCT/TriC
Reactome : Metabolism of proteins
Reactome : Prefoldin mediated transfer of substrate to CCT/TriC
Reactome : Protein folding

Protein

UniProt : A8MWI8
UniProt : Q99832
UniProt : B7Z4Z7
UniProt : B7Z4T9
HPRD : 06896

Clone

Cloning Information : 193370
HIP Master Clone ID : 168081
Original Clone ID : FLH193370.01L

Organism


TAX_ID : 9606
Species Specific ID: 10574

Genome

Chromosome : 2
Map Location : 2p13.2
Ensembl : ENSG00000135624

Reagents

Labome : CCT7-antibody