Gene:
Gene Symbol:
CPSF1
Gene Name:
cleavage and polyadenylation specific factor 1, 160kDa
Original Clone ID:
FLH193594.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00042068
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTACGCCGTGTACAAACAGGCGCATCCGCCCACCGGTCTGGAGTTCTCCATGTACTGC
AACTTCTTCAACAACAGCGAGCGCAACCTGGTAGTGGCCGGGACCTCGCAGCTCTACGTG
TACCGCCTCAACCGCGACGCCGAGGCTCTGACCAAGAATGACAGGAGCACAGAGGGGAAG
GCCCACCGGGAGAAGCTCGAGCTTGCTGCCTCCTTCTCCTTCTTTGGCAACGTCATGTCC
ATGGCCAGCGTGCAGCTGGCAGGAGCCAAGCGGGATGCCCTGCTCCTAAGCTTCAAGGAT
GCCAAGCTGTCTGTGGTGGAGTACGACCCGGGCACCCATGACCTGAAGACCCTGTCACTG
CACTACTTTGAGGAGCCTGAGCTTCGGGACGGGTTTGTGCAGAATGTACACACGCCGCGA
GTGCGGGTGGACCCCGACGGGCGCTGTGCAGCCATGCTTGTCTACGGCACGCGGCTGGTG
GTCCTGCCCTTCCGCAGGGAGAGCCTGGCTGAGGAGCACGAGGGGCTCGTGGGTGAGGGG
CAGAGGTCCAGCTTCCTGCCCAGCTACATCATCGACGTGCGGGCCCTAGACGAGAAGCTG
CTCAACATCATCGACCTGCAGTTCCTGCATGGCTACTACGAGCCTACCCTCCTCATCCTG
TTTGAGCCCAACCAGACCTGGCCTGGGCGCGTGGCCGTGCGGCAGGACACGTGCTCCATT
GTGGCCATCTCACTGAACATCACGCAGAAGGTGCACCCCGTCATCTGGTCCCTCACCAGC
CTGCCCTTTGACTGCACCCAGGCTCTGGCTGTGCCCAAGCCCATAGGTGGGGTGGTGGTG
TTTGCCGTCAACTCGCTGTTGTACCTGAACCAGAGCGTCCCCCCGTATGGCGTGGCTCTC
AACAGCCTCACCACAGGAACCACGGCTTTCCCGCTTCGCACCCAGGAGGGTGTGCGGATC
ACCCTGGACTGCGCCCAGGCCACCTTCATCTCCTACGACAAGATGGTCATCTCCCTCAAG
GGCGGCGAGATCTACGTGCTGACCCTCATCACCGACGGCATGCGCAGTGTCCGAGCGTTC
CACTTTGACAAGGCGGCCGCCAGCGTCCTCACCACCAGCATGGTCACCATGGAGCCCGGG
TACCTGTTCCTGGGTTCTCGCCTGGGCAATTCCCTCCTCCTCAAGTACACGGAGAAGCTG
CAGGAGCCCCCGGCCAGTGCTGTCCGTGAGGCTGCCGACAAGGAAGAGCCTCCCTCAAAG
AAGAAGCGAGTGGATGCGACGGCCGGCTGGTCAGCTGCGGGTAAGTCGGTGCCGCAGGAT
GAGGTGGACGAGATTGAAGTGTACGGCAGCGAGGCCCAGTCGGGAACACAGCTGGCCACC
TACTCCTTTGAGGTGTGTGACAGCATCCTGAACATTGGACCCTGTGCCAATGCCGCCGTG
GGCGAGCCTGCCTTCCTCTCTGAAGAGTTTCAGAACAGCCCCGAGCCGGACCTGGAGATT
GTGGTTTGCTCCGGCCACGGGAAGAACGGGGCTTTGTCGGTGCTGCAGAAGAGCATCCGG
CCCCAGGTGGTGACAACCTTTGAGCTTCCCGGCTGCTATGACATGTGGACAGTCATCGCC
CCGGTGCGTAAGGAGGAGGAGGACAATCCCAAGGGGGAGGGCACAGAGCAGGAACCCAGC
ACCACCCCTGAAGCAGACGACGACGGCCGCAGACACGGATTCCTGATTCTGAGCCGGGAA
GACTCCACCATGATCCTGCAGACGGGGCAGGAGATCATGGAGCTGGACACCAGTGGCTTC
GCCACTCAGGGCCCCACGGTCTTTGCTGGGAACATCGGGGACAACCGCTACATTGTCCAA
GTGTCACCACTGGGCATCCGCCTGCTGGAAGGAGTGAATCAGCTGCACTTCATCCCCGTG
GACCTGGGCGCCCCCATCGTGCAGTGCGCCGTGGCCGACCCCTATGTGGTCATCATGAGT
GCCGAGGGCCACGTCACCATGTTCCTGCTGAAGAGTGACTCCTACGGTGGCCGCCACCAC
CGCCTGGCGCTGCACAAGCCCCCGCTGCACCATCAGTCCAAGGTGATTACGCTGTGCCTG
TACCGAGACCTCAGCGGCATGTTCACCACTGAGAGCCGCCTGGGTGGGGCCCGTGACGAG
CTCGGGGGCCGCAGTGGCCCGGAGGCCGAGGGCCTGGGCTCAGAGACTAGCCCCACAGTG
GATGACGAGGAGGAGATGCTGTATGGGGATTCGGGCTCCCTCTTCAGCCCCAGCAAGGAG
GAGGCCCGAAGAAGCAGCCAGCCCCCTGCTGACCGGGACCCTGCACCCTTCCGGGCAGAG
CCTACCCACTGGTGCCTGCTGGTGCGGGAGAATGGCACCATGGAGATCTACCAGCTTCCC
GACTGGCGGCTGGTGTTCCTGGTGAAGAACTTCCCTGTGGGGCAGCGGGTCCTTGTGGAC
AGCTCCTTTGGACAGCCCACTACACAGGGCGAGGCCCGCAGGGAGGAGGCCACGCGCCAG
GGGGAGCTGCCCCTCGTCAAGGAGGTGCTGCTGGTGGCGCTGGGCAGCCGCCAGAGCAGG
CCCTACCTGCTGGTGCATGTGGACCAAGAGCTGCTTATCTACGAGGCCTTCCCCCACGAC
TCTCAGCTCGGCCAGGGCAATCTCAAAGTCCGCTTTAAGAAGGTCCCTCACAACATCAAC
TTCCGTGAGAAGAAGCCAAAGCCATCCAAGAAGAAAGCAGAAGGTGGCGGCGCAGAGGAG
GGGGCTGGGGCCCGGGGCCGCGTGGCGCGTTTCCGCTACTTCGAGGATATTTATGGCTAC
TCAGGGGTCTTCATCTGCGGCCCCTCCCCTCACTGGCTCTTGGTGACCGGCCGAGGGGCT
CTGCGGCTACACCCCATGGCCATCGACGGCCCGGTCGACTCTTTCGCTCCATTCCACAAT
GTCAACTGTCCCCGCGGCTTCCTGTACTTCAACAGACAGGGCGAGCTGAGGATCAGTGTC
CTGCCTGCCTACCTGTCCTATGATGCCCCATGGCCTGTCAGGAAGATCCCGCTGCGCTGC
ACGGCCCACTATGTGGCTTACCACGTGGAGTCTAAGGTGTATGCTGTGGCCACCAGCACC
AACACGCCGTGTGCCCGCATCCCACGCATGACTGGCGAGGAGAAGGAGTTTGAGACCATC
GAGAGAGATGAGCGGTACATCCACCCCCAGCAGGAGGCCTTCTCCATCCAGCTCATCTCC
CCGGTCAGCTGGGAGGCTATTCCCAATGCCAGGATCGAGCTGCAGGAGTGGGAGCATGTG
ACCTGCATGAAGACAGTGTCTCTGCGCAGTGAGGAGACCGTGTCGGGCCTCAAAGGCTAC
GTGGCCGCCGGGACCTGCCTCATGCAGGGGGAGGAGGTCACGTGCCGAGGGCGGATCTTG
ATCATGGATGTGATTGAGGTGGTGCCCGAGCCTGGCCAGCCCTTGACCAAGAACAAGTTC
AAAGTCCTTTACGAGAAGGAGCAGAAGGGGCCCGTGACCGCCCTGTGCCACTGCAATGGC
CACCTGGTGTCGGCCATCGGCCAGAAGATTTTCCTGTGGAGCCTGCGGGCCAGCGAGCTG
ACGGGCATGGCCTTCATCGACACGCAGCTCTACATACACCAGATGATCAGCGTCAAGAAC
TTCATCCTGGCAGCCGACGTCATGAAGAGCATTTCGCTGCTGCGCTACCAGGAGGAAAGC
AAGACGCTGAGCCTGGTGTCGCGGGATGCCAAGCCCCTGGAGGTGTACAGCGTGGACTTC
ATGGTGGACAATGCCCAGCTGGGTTTTCTGGTGTCTGACCGCGACCGCAACCTCATGGTG
TACATGTACCTGCCCGAAGCCAAGGAGAGTTTCGGGGGCATGCGCCTGCTGCGTCGGGCA
GACTTCCACGTGGGTGCCCACGTGAACACGTTCTGGAGGACCCCGTGCCGGGGGGCCACT
GAAGGGCTCAGCAAAAAGTCGGTCGTGTGGGAGAATAAGCACATCACGTGGTTTGCCACC
CTGGACGGCGGCATCGGGCTGCTGCTGCCCATGCAGGAGAAGACCTACCGGCGGCTGCTG
ATGCTGCAGAACGCGCTGACCACCATGCTGCCACACCACGCCGGCCTCAACCCCCGCGCC
TTCCGGATGCTGCACGTGGACCGCCGCACCCTCCAGAATGCCGTGCGCAACGTGCTGGAT
GGGGAGCTGCTCAACCGCTACCTGTACCTGAGCACCATGGAGCGCAGCGAGCTAGCCAAG
AAGATCGGCACCACACCAGACATAATCCTGGACGACTTGCTGGAGACGGACCGCGTCACC
GCCCACTTC
Protein sequence predicted based on nucleotide sequence
MYAVYKQAHPPTGLEFSMYCNFFNNSERNLVVAGTSQLYVYRLNRDAEALTKNDRSTEGK AHREKLELAASFSFFGNVMSMASVQLAGAKRDALLLSFKDAKLSVVEYDPGTHDLKTLSL HYFEEPELRDGFVQNVHTPRVRVDPDGRCAAMLVYGTRLVVLPFRRESLAEEHEGLVGEG QRSSFLPSYIIDVRALDEKLLNIIDLQFLHGYYEPTLLILFEPNQTWPGRVAVRQDTCSI VAISLNITQKVHPVIWSLTSLPFDCTQALAVPKPIGGVVVFAVNSLLYLNQSVPPYGVAL NSLTTGTTAFPLRTQEGVRITLDCAQATFISYDKMVISLKGGEIYVLTLITDGMRSVRAF HFDKAAASVLTTSMVTMEPGYLFLGSRLGNSLLLKYTEKLQEPPASAVREAADKEEPPSK KKRVDATAGWSAAGKSVPQDEVDEIEVYGSEAQSGTQLATYSFEVCDSILNIGPCANAAV GEPAFLSEEFQNSPEPDLEIVVCSGHGKNGALSVLQKSIRPQVVTTFELPGCYDMWTVIA PVRKEEEDNPKGEGTEQEPSTTPEADDDGRRHGFLILSREDSTMILQTGQEIMELDTSGF ATQGPTVFAGNIGDNRYIVQVSPLGIRLLEGVNQLHFIPVDLGAPIVQCAVADPYVVIMS AEGHVTMFLLKSDSYGGRHHRLALHKPPLHHQSKVITLCLYRDLSGMFTTESRLGGARDE LGGRSGPEAEGLGSETSPTVDDEEEMLYGDSGSLFSPSKEEARRSSQPPADRDPAPFRAE PTHWCLLVRENGTMEIYQLPDWRLVFLVKNFPVGQRVLVDSSFGQPTTQGEARREEATRQ GELPLVKEVLLVALGSRQSRPYLLVHVDQELLIYEAFPHDSQLGQGNLKVRFKKVPHNIN FREKKPKPSKKKAEGGGAEEGAGARGRVARFRYFEDIYGYSGVFICGPSPHWLLVTGRGA LRLHPMAIDGPVDSFAPFHNVNCPRGFLYFNRQGELRISVLPAYLSYDAPWPVRKIPLRC TAHYVAYHVESKVYAVATSTNTPCARIPRMTGEEKEFETIERDERYIHPQQEAFSIQLIS PVSWEAIPNARIELQEWEHVTCMKTVSLRSEETVSGLKGYVAAGTCLMQGEEVTCRGRIL IMDVIEVVPEPGQPLTKNKFKVLYEKEQKGPVTALCHCNGHLVSAIGQKIFLWSLRASEL TGMAFIDTQLYIHQMISVKNFILAADVMKSISLLRYQEESKTLSLVSRDAKPLEVYSVDF MVDNAQLGFLVSDRDRNLMVYMYLPEAKESFGGMRLLRRADFHVGAHVNTFWRTPCRGAT EGLSKKSVVWENKHITWFATLDGGIGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPRA FRMLHVDRRTLQNAVRNVLDGELLNRYLYLSTMERSELAKKIGTTPDIILDDLLETDRVT AHF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4386
Start on reference sequence
55
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
