DNASU Plasmid Repository • 480.965.5697 | Email

AK1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  AK1
Gene Name:  adenylate kinase 1
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH020265.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors: HIP
HsCD00000421

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 585nts         Open reading frame : 1 to 585
Reference Sequence Alignment

ATGGAAGAGAAGCTGAAGAAAACCAAGATCATCTTTGTGGTGGGTGGGCCTGGCTCAGGG
AAGGGCACCCAGTGTGAGAAGATCGTGCAGAAGTATGGCTACACCCACCTCTCCACCGGG
GACCTCCTGCGGTCCGAGGTCAGCTCAGGCTCGGCCAGGGGCAAGAAGCTGTCGGAAATC
ATGGAAAAGGGGCAGCTGGTTCCACTGGAGACAGTGTTGGACATGCTCCGGGATGCCATG
GTGGCCAAAGTCAATACTTCCAAAGGCTTCCTGATTGATGGCTACCCGCGGGAGGTGCAG
CAAGGAGAAGAGTTTGAGCGACGGATTGGACAGCCCACACTGCTGCTGTATGTGGACGCA
GGCCCTGAGACCATGACCCAGCGGCTCTTGAAACGTGGAGAGACCAGCGGGCGTGTGGAC
GACAATGAGGAGACCATCAAAAAGCGGCTGGAGACCTATTACAAGGCCACAGAACCCGTC
ATCGCCTTCTATGAGAAACGTGGCATTGTGCGCAAGGTCAACGCTGAGGGCTCCGTGGAC
AGTGTCTTCTCCCAGGTCTGCACCCACCTGGACGCCCTAAAGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 659
Start on reference sequence 75
Mutations:
None
Discrepancy:
g186a
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : AK1
Symbol Nomenclature : AK1
SYNONYM : HTL-S-58j
Designation : AK 1|ATP-AMP transphosphorylase 1|ATP:AMP phosphotransferase|adenylate kinase isoenzyme 1|adenylate monophosphate kinase|myokinase|testis secretory sperm binding protein Li 58j
Full Nomenclature : adenylate kinase 1
GENEID : 203
Locus Tag : RP11-203J24.1
GI : 61355726
GenBank Accession : AY888224
HGNC : 361
MIM : 103000
Vega : OTTHUMG00000020722
Target GenBank: NM_000476

 

Reference Sequence Alignment

  

HsCD00000421        ------------------------------------------------ATGGAAGAGAAG
XM_005251788.1      ------------------------------------------------------------
NM_000476.2         ------------------------------------------------ATGGAAGAGAAG
XM_005251786.1      ATGGGCTGCTGCTCCTCGAGTGACCCCCGCAGAGAAGACGATCTGAGAGCCAGAGAGAAG
                                                                                

HsCD00000421        CTGAAGAAAACCAAGATCATCTTTGTGGTGGGTGGGCCTGGCTCAGGGAAGGGCACCCAG
XM_005251788.1      ---------ATGAATGGGTGGGCGTTTGCAGGTGGGCCTGGCTCAGGGAAGGGCACCCAG
NM_000476.2         CTGAAGAAAACCAAGATCATCTTTGTGGTGGGTGGGCCTGGCTCAGGGAAGGGCACCCAG
XM_005251786.1      CTGAAGAAAACCAAGATCATCTTTGTGGTGGGTGGGCCTGGCTCAGGGAAGGGCACCCAG
                             *  ** .  :      * * .******************************

HsCD00000421        TGTGAGAAGATCGTGCAGAAGTATGGCTACACCCACCTCTCCACCGGGGACCTCCTGCGG
XM_005251788.1      TGTGAGAAGATCGTGCAGAAGTATGGCTACACCCACCTCTCCACCGGGGACCTCCTGCGG
NM_000476.2         TGTGAGAAGATCGTGCAGAAGTATGGCTACACCCACCTCTCCACCGGGGACCTCCTGCGG
XM_005251786.1      TGTGAGAAGATCGTGCAGAAGTATGGCTACACCCACCTCTCCACCGGGGACCTCCTGCGG
                    ************************************************************

HsCD00000421        TCCGAGGTCAGCTCAGGCTCGGCCAGGGGCAAGAAGCTGTCGGAAATCATGGAAAAGGGG
XM_005251788.1      TCCGAGGTCAGCTCAGGCTCGGCCAGGGGCAAGAAGCTGTCGGAAATCATGGAGAAGGGG
NM_000476.2         TCCGAGGTCAGCTCAGGCTCGGCCAGGGGCAAGAAGCTGTCGGAAATCATGGAGAAGGGG
XM_005251786.1      TCCGAGGTCAGCTCAGGCTCGGCCAGGGGCAAGAAGCTGTCGGAAATCATGGAGAAGGGG
                    *****************************************************.******

HsCD00000421        CAGCTGGTTCCACTGGAGACAGTGTTGGACATGCTCCGGGATGCCATGGTGGCCAAAGTC
XM_005251788.1      CAGCTGGTTCCACTGGAGACAGTGTTGGACATGCTCCGGGATGCCATGGTGGCCAAAGTC
NM_000476.2         CAGCTGGTTCCACTGGAGACAGTGTTGGACATGCTCCGGGATGCCATGGTGGCCAAAGTC
XM_005251786.1      CAGCTGGTTCCACTGGAGACAGTGTTGGACATGCTCCGGGATGCCATGGTGGCCAAAGTC
                    ************************************************************

HsCD00000421        AATACTTCCAAAGGCTTCCTGATTGATGGCTACCCGCGGGAGGTGCAGCAAGGAGAAGAG
XM_005251788.1      AATACTTCCAAAGGCTTCCTGATTGATGGCTACCCGCGGGAGGTGCAGCAAGGAGAAGAG
NM_000476.2         AATACTTCCAAAGGCTTCCTGATTGATGGCTACCCGCGGGAGGTGCAGCAAGGAGAAGAG
XM_005251786.1      AATACTTCCAAAGGCTTCCTGATTGATGGCTACCCGCGGGAGGTGCAGCAAGGAGAAGAG
                    ************************************************************

HsCD00000421        TTTGAGCGACGGATTGGACAGCCCACACTGCTGCTGTATGTGGACGCAGGCCCTGAGACC
XM_005251788.1      TTTGAGCGACGGATTGGACAGCCCACACTGCTGCTGTATGTGGACGCAGGCCCTGAGACC
NM_000476.2         TTTGAGCGACGGATTGGACAGCCCACACTGCTGCTGTATGTGGACGCAGGCCCTGAGACC
XM_005251786.1      TTTGAGCGACGGATTGGACAGCCCACACTGCTGCTGTATGTGGACGCAGGCCCTGAGACC
                    ************************************************************

HsCD00000421        ATGACCCAGCGGCTCTTGAAACGTGGAGAGACCAGCGGGCGTGTGGACGACAATGAGGAG
XM_005251788.1      ATGACCCAGCGGCTCTTGAAACGTGGAGAGACCAGCGGGCGTGTGGACGACAATGAGGAG
NM_000476.2         ATGACCCAGCGGCTCTTGAAACGTGGAGAGACCAGCGGGCGTGTGGACGACAATGAGGAG
XM_005251786.1      ATGACCCAGCGGCTCTTGAAACGTGGAGAGACCAGCGGGCGTGTGGACGACAATGAGGAG
                    ************************************************************

HsCD00000421        ACCATCAAAAAGCGGCTGGAGACCTATTACAAGGCCACAGAACCCGTCATCGCCTTCTAT
XM_005251788.1      ACCATCAAAAAGCGGCTGGAGACCTATTACAAGGCCACAGAACCTGTCATCGCCTTCTAT
NM_000476.2         ACCATCAAAAAGCGGCTGGAGACCTATTACAAGGCCACAGAACCCGTCATCGCCTTCTAT
XM_005251786.1      ACCATCAAAAAGCGGCTGGAGACCTATTACAAGGCCACAGAACCTGTCATCGCCTTCTAT
                    ******************************************** ***************

HsCD00000421        GAGAAACGTGGCATTGTGCGCAAGGTCAACGCTGAGGGCTCCGTGGACAGTGTCTTCTCC
XM_005251788.1      GAGAAACGTGGCATTGTGCGCAAGGTCAACGCTGAGGGCTCCGTGGACAGTGTCTTCTCC
NM_000476.2         GAGAAACGTGGCATTGTGCGCAAGGTCAACGCTGAGGGCTCCGTGGACAGTGTCTTCTCC
XM_005251786.1      GAGAAACGTGGCATTGTGCGCAAGGTCAACGCTGAGGGCTCCGTGGACAGTGTCTTCTCC
                    ************************************************************

HsCD00000421        CAGGTCTGCACCCACCTGGACGCCCTAAAGTAG
XM_005251788.1      CAGGTCTGCACCCACCTGGACGCCCTAAAGTAG
NM_000476.2         CAGGTCTGCACCCACCTGGACGCCCTAAAGTAG
XM_005251786.1      CAGGTCTGCACCCACCTGGACGCCCTAAAGTAG
                    *********************************

Pathways

Panther : De novo purine biosynthesis
Reactome : Metabolism
Reactome : Metabolism of nucleotides
Reactome : Synthesis and interconversion of nucleotide di- and triphosphates

Protein

UniProt : Q6FGX9
UniProt : P00568
HPRD : 00046

Clone

Cloning Information : 20265
HIP Master Clone ID : 36361
Original Clone ID : FLH020265.01X

Organism


TAX_ID : 9606
Species Specific ID: 203

Genome

Chromosome : 9
Map Location : 9q34.1
Ensembl : ENSG00000106992

Reagents

Labome : AK1-antibody