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LUM (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LUM
Gene Name:  lumican
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH025903.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00004228

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1017nts         Open reading frame : 1 to 1017
Reference Sequence Alignment

ATGAGTCTAAGTGCATTTACTCTCTTCCTGGCATTGATTGGTGGTACCAGTGGCCAGTAC
TATGATTATGATTTTCCCCTATCAATTTATGGGCAATCATCACCAAACTGTGCACCAGAA
TGTAACTGCCCTGAAAGCTACCCAAGTGCCATGTACTGTGATGAGCTGAAATTGAAAAGT
GTACCAATGGTGCCTCCTGGAATCAAGTATCTTTACCTTAGGAATAACCAGATTGACCAT
ATTGATGAAAAGGCCTTTGAGAATGTAACTGATCTGCAGTGGCTCATTCTAGATCACAAC
CTTCTAGAAAACTCCAAGATAAAAGGGAGAGTTTTCTCTAAATTGAAACAACTGAAGAAG
CTGCATATAAACCACAACAACCTGACAGAGTCTGTGGGCCCACTTCCCAAATCTCTGGAG
GATCTGCAGCTTACTCATAACAAGATCACAAAGCTGGGCTCTTTTGAAGGATTGGTAAAC
CTGACCTTCATCCATCTCCAGCACAATCGGCTGAAAGAGGATGCTGTTTCAGCTGCTTTT
AAAGGTCTTAAATCACTCGAATACCTTGACTTGAGCTTCAATCAGATAGCCAGACTGCCT
TCTGGTCTCCCTGTCTCTCTTCTAACTCTCTACTTAGACAACAATAAGATCAGCAACATC
CCTGATGAGTATTTCAAGCGTTTTAATGCATTGCAGTATCTGCGTTTATCTCACAACGAA
CTGGCTGATAGTGGAATACCTGGAAATTCTTTCAATGTGTCATCCCTGGTTGAGCTGGAT
CTGTCCTATAACAAGCTTAAAAACATACCAACTGTCAATGAAAACCTTGAAAACTATTAC
CTGGAGGTCAATCAACTTGAGAAGTTTGACATAAAGAGCTTCTGCAAGATCCTGGGGCCA
TTATCCTACTCCAAGATCAAGCATTTGCGTTTGGATGGCAATCGCATCTCAGAAACCAGT
CTTCCACCGGATATGTATGAATGTCTACGTGTTGCTAACGAAGTCACTCTTAATTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1158
Start on reference sequence 142
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LUM
Symbol Nomenclature : LUM
SYNONYM : LDC; SLRR2D
Designation : KSPG lumican|keratan sulfate proteoglycan lumican|lumican proteoglycan
Full Nomenclature : lumican
GENEID : 4060
GI : 60656530
GenBank Accession : AY890038
HGNC : 6724
MIM : 600616
Target GenBank: BC007038

 

Reference Sequence Alignment

  

HsCD00004228      ATGAGTCTAAGTGCATTTACTCTCTTCCTGGCATTGATTGGTGGTACCAGTGGCCAGTAC
NM_002345.3       ATGAGTCTAAGTGCATTTACTCTCTTCCTGGCATTGATTGGTGGTACCAGTGGCCAGTAC
                  ************************************************************

HsCD00004228      TATGATTATGATTTTCCCCTATCAATTTATGGGCAATCATCACCAAACTGTGCACCAGAA
NM_002345.3       TATGATTATGATTTTCCCCTATCAATTTATGGGCAATCATCACCAAACTGTGCACCAGAA
                  ************************************************************

HsCD00004228      TGTAACTGCCCTGAAAGCTACCCAAGTGCCATGTACTGTGATGAGCTGAAATTGAAAAGT
NM_002345.3       TGTAACTGCCCTGAAAGCTACCCAAGTGCCATGTACTGTGATGAGCTGAAATTGAAAAGT
                  ************************************************************

HsCD00004228      GTACCAATGGTGCCTCCTGGAATCAAGTATCTTTACCTTAGGAATAACCAGATTGACCAT
NM_002345.3       GTACCAATGGTGCCTCCTGGAATCAAGTATCTTTACCTTAGGAATAACCAGATTGACCAT
                  ************************************************************

HsCD00004228      ATTGATGAAAAGGCCTTTGAGAATGTAACTGATCTGCAGTGGCTCATTCTAGATCACAAC
NM_002345.3       ATTGATGAAAAGGCCTTTGAGAATGTAACTGATCTGCAGTGGCTCATTCTAGATCACAAC
                  ************************************************************

HsCD00004228      CTTCTAGAAAACTCCAAGATAAAAGGGAGAGTTTTCTCTAAATTGAAACAACTGAAGAAG
NM_002345.3       CTTCTAGAAAACTCCAAGATAAAAGGGAGAGTTTTCTCTAAATTGAAACAACTGAAGAAG
                  ************************************************************

HsCD00004228      CTGCATATAAACCACAACAACCTGACAGAGTCTGTGGGCCCACTTCCCAAATCTCTGGAG
NM_002345.3       CTGCATATAAACCACAACAACCTGACAGAGTCTGTGGGCCCACTTCCCAAATCTCTGGAG
                  ************************************************************

HsCD00004228      GATCTGCAGCTTACTCATAACAAGATCACAAAGCTGGGCTCTTTTGAAGGATTGGTAAAC
NM_002345.3       GATCTGCAGCTTACTCATAACAAGATCACAAAGCTGGGCTCTTTTGAAGGATTGGTAAAC
                  ************************************************************

HsCD00004228      CTGACCTTCATCCATCTCCAGCACAATCGGCTGAAAGAGGATGCTGTTTCAGCTGCTTTT
NM_002345.3       CTGACCTTCATCCATCTCCAGCACAATCGGCTGAAAGAGGATGCTGTTTCAGCTGCTTTT
                  ************************************************************

HsCD00004228      AAAGGTCTTAAATCACTCGAATACCTTGACTTGAGCTTCAATCAGATAGCCAGACTGCCT
NM_002345.3       AAAGGTCTTAAATCACTCGAATACCTTGACTTGAGCTTCAATCAGATAGCCAGACTGCCT
                  ************************************************************

HsCD00004228      TCTGGTCTCCCTGTCTCTCTTCTAACTCTCTACTTAGACAACAATAAGATCAGCAACATC
NM_002345.3       TCTGGTCTCCCTGTCTCTCTTCTAACTCTCTACTTAGACAACAATAAGATCAGCAACATC
                  ************************************************************

HsCD00004228      CCTGATGAGTATTTCAAGCGTTTTAATGCATTGCAGTATCTGCGTTTATCTCACAACGAA
NM_002345.3       CCTGATGAGTATTTCAAGCGTTTTAATGCATTGCAGTATCTGCGTTTATCTCACAACGAA
                  ************************************************************

HsCD00004228      CTGGCTGATAGTGGAATACCTGGAAATTCTTTCAATGTGTCATCCCTGGTTGAGCTGGAT
NM_002345.3       CTGGCTGATAGTGGAATACCTGGAAATTCTTTCAATGTGTCATCCCTGGTTGAGCTGGAT
                  ************************************************************

HsCD00004228      CTGTCCTATAACAAGCTTAAAAACATACCAACTGTCAATGAAAACCTTGAAAACTATTAC
NM_002345.3       CTGTCCTATAACAAGCTTAAAAACATACCAACTGTCAATGAAAACCTTGAAAACTATTAC
                  ************************************************************

HsCD00004228      CTGGAGGTCAATCAACTTGAGAAGTTTGACATAAAGAGCTTCTGCAAGATCCTGGGGCCA
NM_002345.3       CTGGAGGTCAATCAACTTGAGAAGTTTGACATAAAGAGCTTCTGCAAGATCCTGGGGCCA
                  ************************************************************

HsCD00004228      TTATCCTACTCCAAGATCAAGCATTTGCGTTTGGATGGCAATCGCATCTCAGAAACCAGT
NM_002345.3       TTATCCTACTCCAAGATCAAGCATTTGCGTTTGGATGGCAATCGCATCTCAGAAACCAGT
                  ************************************************************

HsCD00004228      CTTCCACCGGATATGTATGAATGTCTACGTGTTGCTAACGAAGTCACTCTTAATTAG
NM_002345.3       CTTCCACCGGATATGTATGAATGTCTACGTGTTGCTAACGAAGTCACTCTTAATTAA
                  ********************************************************.

Pathways

Reactome : Disease
Reactome : ECM proteoglycans
Reactome : Extracellular matrix organization
Reactome : Glycogen storage diseases
Reactome : Glycosaminoglycan metabolism
Reactome : Integrin cell surface interactions
Reactome : Keratan sulfate biosynthesis
Reactome : Keratan sulfate degradation
Reactome : Keratan sulfate/keratin metabolism
Reactome : MPS I - Hurler syndrome
Reactome : MPS II - Hunter syndrome
Reactome : MPS IIIA - Sanfilippo syndrome A
Reactome : MPS IIIB - Sanfilippo syndrome B
Reactome : MPS IIIC - Sanfilippo syndrome C
Reactome : MPS IIID - Sanfilippo syndrome D
Reactome : MPS IV - Morquio syndrome A
Reactome : MPS IV - Morquio syndrome B
Reactome : MPS IX - Natowicz syndrome
Reactome : MPS VI - Maroteaux-Lamy syndrome
Reactome : MPS VII - Sly syndrome
Reactome : Metabolism
Reactome : Metabolism of carbohydrates
Reactome : Mucopolysaccharidoses
Reactome : Myoclonic epilepsy of Lafora

Clone

Cloning Information : 25903
HIP Master Clone ID : 56279
Original Clone ID : FLH025903.01X

Organism


TAX_ID : 9606
Species Specific ID: 4060

Genome

Chromosome : 12
Map Location : 12q21.3-q22

Reagents

Labome : lumican-antibody