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B3GAT1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  B3GAT1
Gene Name:  beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH196169.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00042326

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1005nts         Open reading frame : 1 to 1005
Reference Sequence Alignment

ATGCCGAAGAGACGGGACATCCTAGCGATCGTCCTCATCGTGCTGCCCTGGACTCTGCTC
ATCACTGTCTGGCACCAGAGCACCCTCGCACCCCTGCTCGCGGTACATAAGGATGAGGGC
AGTGACCCCCGACGCGAAACGCCGCCCGGCGCCGACCCCAGGGAGTACTGCACGTCTGAC
CGCGACATCGTGGAGGTGGTGCGCACCGAGTACGTGTACACGCGGCCCCCGCCATGGTCC
GACACGCTGCCCACCATCCACGTGGTGACGCCCACCTACAGCCGCCCGGTGCAGAAGGCC
GAGCTGACGCGCATGGCCAACACGCTGCTGCACGTGCCCAACCTCCACTGGCTGGTGGTG
GAGGATGCGCCGCGCCGGACGCCGCTGACCGCGCGCCTGCTGCGCGACACCGGCCTCAAC
TACACGCACCTGCACGTGGAGACGCCCCGCAACTACAAGCTGCGCGGAGACGCCCGCGAC
CCACGCATCCCGCGGGGCACCATGCAGCGCAACCTGGCCCTGCGCTGGCTGCGCGAGACC
TTCCCGCGCAACTCCAGCCAGCCTGGCGTGGTCTACTTCGCCGACGACGACAACACCTAC
AGCCTGGAGCTCTTCGAAGAGATGCGCAGCACCAGGAGGGTGTCCGTGTGGCCCGTCGCC
TTCGTGGGTGGCCTGCGGTACGAGGCCCCACGGGTGAACGGGGCAGGGAAGGTGGTCGGC
TGGAAGACGGTGTTTGACCCCCACCGGCCATTTGCAATAGACATGGCTGGATTTGCCGTC
AACCTGCGGCTCATTCTGCAGCGAAGCCAGGCCTACTTCAAGCTGCGAGGTGTGAAGGGA
GGCTACCAGGAAAGCAGCCTCCTTCGAGAACTTGTCACCCTCAACGACCTGGAGCCCAAG
GCAGCCAACTGCACCAAGATCCTGGTGTGGCACACACGGACAGAGAAGCCAGTGCTGGTG
AATGAGGGCAAGAAGGGCTTCACTGACCCCTCGGTGGAGATCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1398
Start on reference sequence 394
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : B3GAT1
Symbol Nomenclature : B3GAT1
SYNONYM : CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1
Designation : LEU7 antigen|UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase|galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1|glcUAT-P|glucuronosyltransferase P
Full Nomenclature : beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
GENEID : 27087
HGNC : 921
MIM : 151290
Vega : OTTHUMG00000167180
Target GenBank: BC010466

 

Reference Sequence Alignment

  

HsCD00042326        ---------------------------------------ATGCCGAAGAGACGGGACATC
NM_054025.2         ---------------------------------------ATGCCGAAGAGACGGGACATC
XM_005271506.1      ATGGGTAATGAGGAGCCGTGGGTGCAGCCAGCCTTGGAGATGCCGAAGAGACGGGACATC
NM_018644.3         ---------------------------------------ATGCCGAAGAGACGGGACATC
                                                           *********************

HsCD00042326        CTAGCGATCGTCCTCATCGTGCTGCCCTGGACTCTGCTCATCACTGTCTGGCACCAGAGC
NM_054025.2         CTAGCGATCGTCCTCATCGTGCTGCCCTGGACTCTGCTCATCACTGTCTGGCACCAGAGC
XM_005271506.1      CTAGCGATCGTCCTCATCGTGCTGCCCTGGACTCTGCTCATCACTGTCTGGCACCAGAGC
NM_018644.3         CTAGCGATCGTCCTCATCGTGCTGCCCTGGACTCTGCTCATCACTGTCTGGCACCAGAGC
                    ************************************************************

HsCD00042326        ACCCTCGCACCCCTGCTCGCGGTACATAAGGATGAGGGCAGTGACCCCCGACGCGAAACG
NM_054025.2         ACCCTCGCACCCCTGCTCGCGGTACATAAGGATGAGGGCAGTGACCCCCGACGCGAAACG
XM_005271506.1      ACCCTCGCACCCCTGCTCGCGGTACATAAGGATGAGGGCAGTGACCCCCGACGCGAAACG
NM_018644.3         ACCCTCGCACCCCTGCTCGCGGTACATAAGGATGAGGGCAGTGACCCCCGACGCGAAACG
                    ************************************************************

HsCD00042326        CCGCCCGGCGCCGACCCCAGGGAGTACTGCACGTCTGACCGCGACATCGTGGAGGTGGTG
NM_054025.2         CCGCCCGGCGCCGACCCCAGGGAGTACTGCACGTCTGACCGCGACATCGTGGAGGTGGTG
XM_005271506.1      CCGCCCGGCGCCGACCCCAGGGAGTACTGCACGTCTGACCGCGACATCGTGGAGGTGGTG
NM_018644.3         CCGCCCGGCGCCGACCCCAGGGAGTACTGCACGTCTGACCGCGACATCGTGGAGGTGGTG
                    ************************************************************

HsCD00042326        CGCACCGAGTACGTGTACACGCGGCCCCCGCCATGGTCCGACACGCTGCCCACCATCCAC
NM_054025.2         CGCACCGAGTACGTGTACACGCGGCCCCCGCCATGGTCCGACACGCTGCCCACCATCCAC
XM_005271506.1      CGCACCGAGTACGTGTACACGCGGCCCCCGCCATGGTCCGACACGCTGCCCACCATCCAC
NM_018644.3         CGCACCGAGTACGTGTACACGCGGCCCCCGCCATGGTCCGACACGCTGCCCACCATCCAC
                    ************************************************************

HsCD00042326        GTGGTGACGCCCACCTACAGCCGCCCGGTGCAGAAGGCCGAGCTGACGCGCATGGCCAAC
NM_054025.2         GTGGTGACGCCCACCTACAGCCGCCCGGTGCAGAAGGCCGAGCTGACGCGCATGGCCAAC
XM_005271506.1      GTGGTGACGCCCACCTACAGCCGCCCGGTGCAGAAGGCCGAGCTGACGCGCATGGCCAAC
NM_018644.3         GTGGTGACGCCCACCTACAGCCGCCCGGTGCAGAAGGCCGAGCTGACGCGCATGGCCAAC
                    ************************************************************

HsCD00042326        ACGCTGCTGCACGTGCCCAACCTCCACTGGCTGGTGGTGGAGGATGCGCCGCGCCGGACG
NM_054025.2         ACGCTGCTGCACGTGCCCAACCTCCACTGGCTGGTGGTGGAGGATGCGCCGCGCCGGACG
XM_005271506.1      ACGCTGCTGCACGTGCCCAACCTCCACTGGCTGGTGGTGGAGGATGCGCCGCGCCGGACG
NM_018644.3         ACGCTGCTGCACGTGCCCAACCTCCACTGGCTGGTGGTGGAGGATGCGCCGCGCCGGACG
                    ************************************************************

HsCD00042326        CCGCTGACCGCGCGCCTGCTGCGCGACACCGGCCTCAACTACACGCACCTGCACGTGGAG
NM_054025.2         CCGCTGACCGCGCGCCTGCTGCGCGACACCGGCCTCAACTACACGCACCTGCACGTGGAG
XM_005271506.1      CCGCTGACCGCGCGCCTGCTGCGCGACACCGGCCTCAACTACACGCACCTGCACGTGGAG
NM_018644.3         CCGCTGACCGCGCGCCTGCTGCGCGACACCGGCCTCAACTACACGCACCTGCACGTGGAG
                    ************************************************************

HsCD00042326        ACGCCCCGCAACTACAAGCTGCGCGGAGACGCCCGCGACCCACGCATCCCGCGGGGCACC
NM_054025.2         ACGCCCCGCAACTACAAGCTGCGCGGAGACGCCCGCGACCCACGCATCCCGCGGGGCACC
XM_005271506.1      ACGCCCCGCAACTACAAGCTGCGCGGAGACGCCCGCGACCCACGCATCCCGCGGGGCACC
NM_018644.3         ACGCCCCGCAACTACAAGCTGCGCGGAGACGCCCGCGACCCACGCATCCCGCGGGGCACC
                    ************************************************************

HsCD00042326        ATGCAGCGCAACCTGGCCCTGCGCTGGCTGCGCGAGACCTTCCCGCGCAACTCCAGCCAG
NM_054025.2         ATGCAGCGCAACCTGGCCCTGCGCTGGCTGCGCGAGACCTTCCCGCGCAACTCCAGCCAG
XM_005271506.1      ATGCAGCGCAACCTGGCCCTGCGCTGGCTGCGCGAGACCTTCCCGCGCAACTCCAGCCAG
NM_018644.3         ATGCAGCGCAACCTGGCCCTGCGCTGGCTGCGCGAGACCTTCCCGCGCAACTCCAGCCAG
                    ************************************************************

HsCD00042326        CCTGGCGTGGTCTACTTCGCCGACGACGACAACACCTACAGCCTGGAGCTCTTCGAAGAG
NM_054025.2         CCTGGCGTGGTCTACTTCGCCGACGACGACAACACCTACAGCCTGGAGCTCTTCGAAGAG
XM_005271506.1      CCTGGCGTGGTCTACTTCGCCGACGACGACAACACCTACAGCCTGGAGCTCTTCGAAGAG
NM_018644.3         CCTGGCGTGGTCTACTTCGCCGACGACGACAACACCTACAGCCTGGAGCTCTTCGAAGAG
                    ************************************************************

HsCD00042326        ATGCGCAGCACCAGGAGGGTGTCCGTGTGGCCCGTCGCCTTCGTGGGTGGCCTGCGGTAC
NM_054025.2         ATGCGCAGCACCAGGAGGGTGTCCGTGTGGCCCGTCGCCTTCGTGGGTGGCCTGCGGTAC
XM_005271506.1      ATGCGCAGCACCAGGAGGGTGTCCGTGTGGCCCGTCGCCTTCGTGGGTGGCCTGCGGTAC
NM_018644.3         ATGCGCAGCACCAGGAGGGTGTCCGTGTGGCCCGTCGCCTTCGTGGGTGGCCTGCGGTAC
                    ************************************************************

HsCD00042326        GAGGCCCCACGGGTGAACGGGGCAGGGAAGGTGGTCGGCTGGAAGACGGTGTTTGACCCC
NM_054025.2         GAGGCCCCACGGGTGAACGGGGCAGGGAAGGTGGTCGGCTGGAAGACGGTGTTTGACCCC
XM_005271506.1      GAGGCCCCACGGGTGAACGGGGCAGGGAAGGTGGTCGGCTGGAAGACGGTGTTTGACCCC
NM_018644.3         GAGGCCCCACGGGTGAACGGGGCAGGGAAGGTGGTCGGCTGGAAGACGGTGTTTGACCCC
                    ************************************************************

HsCD00042326        CACCGGCCATTTGCAATAGACATGGCTGGATTTGCCGTCAACCTGCGGCTCATTCTGCAG
NM_054025.2         CACCGGCCATTTGCAATAGACATGGCTGGATTTGCCGTCAACCTGCGGCTCATTCTGCAG
XM_005271506.1      CACCGGCCATTTGCAATAGACATGGCTGGATTTGCCGTCAACCTGCGGCTCATTCTGCAG
NM_018644.3         CACCGGCCATTTGCAATAGACATGGCTGGATTTGCCGTCAACCTGCGGCTCATTCTGCAG
                    ************************************************************

HsCD00042326        CGAAGCCAGGCCTACTTCAAGCTGCGAGGTGTGAAGGGAGGCTACCAGGAAAGCAGCCTC
NM_054025.2         CGAAGCCAGGCCTACTTCAAGCTGCGAGGTGTGAAGGGAGGCTACCAGGAAAGCAGCCTC
XM_005271506.1      CGAAGCCAGGCCTACTTCAAGCTGCGAGGTGTGAAGGGAGGCTACCAGGAAAGCAGCCTC
NM_018644.3         CGAAGCCAGGCCTACTTCAAGCTGCGAGGTGTGAAGGGAGGCTACCAGGAAAGCAGCCTC
                    ************************************************************

HsCD00042326        CTTCGAGAACTTGTCACCCTCAACGACCTGGAGCCCAAGGCAGCCAACTGCACCAAGATC
NM_054025.2         CTTCGAGAACTTGTCACCCTCAACGACCTGGAGCCCAAGGCAGCCAACTGCACCAAGATC
XM_005271506.1      CTTCGAGAACTTGTCACCCTCAACGACCTGGAGCCCAAGGCAGCCAACTGCACCAAGATC
NM_018644.3         CTTCGAGAACTTGTCACCCTCAACGACCTGGAGCCCAAGGCAGCCAACTGCACCAAGATC
                    ************************************************************

HsCD00042326        CTGGTGTGGCACACACGGACAGAGAAGCCAGTGCTGGTGAATGAGGGCAAGAAGGGCTTC
NM_054025.2         CTGGTGTGGCACACACGGACAGAGAAGCCAGTGCTGGTGAATGAGGGCAAGAAGGGCTTC
XM_005271506.1      CTGGTGTGGCACACACGGACAGAGAAGCCAGTGCTGGTGAATGAGGGCAAGAAGGGCTTC
NM_018644.3         CTGGTGTGGCACACACGGACAGAGAAGCCAGTGCTGGTGAATGAGGGCAAGAAGGGCTTC
                    ************************************************************

HsCD00042326        ACTGACCCCTCGGTGGAGATCTTG
NM_054025.2         ACTGACCCCTCGGTGGAGATCTGA
XM_005271506.1      ACTGACCCCTCGGTGGAGATCTGA
NM_018644.3         ACTGACCCCTCGGTGGAGATCTGA
                    ********************** .

Pathways

Reactome : A tetrasaccharide linker sequence is required for GAG synthesis
Reactome : Chondroitin sulfate/dermatan sulfate metabolism
Reactome : Disease
Reactome : Glycogen storage diseases
Reactome : Glycosaminoglycan metabolism
Reactome : Heparan sulfate/heparin (HS-GAG) metabolism
Reactome : MPS I - Hurler syndrome
Reactome : MPS II - Hunter syndrome
Reactome : MPS IIIA - Sanfilippo syndrome A
Reactome : MPS IIIB - Sanfilippo syndrome B
Reactome : MPS IIIC - Sanfilippo syndrome C
Reactome : MPS IIID - Sanfilippo syndrome D
Reactome : MPS IV - Morquio syndrome A
Reactome : MPS IV - Morquio syndrome B
Reactome : MPS IX - Natowicz syndrome
Reactome : MPS VI - Maroteaux-Lamy syndrome
Reactome : MPS VII - Sly syndrome
Reactome : Metabolism
Reactome : Metabolism of carbohydrates
Reactome : Mucopolysaccharidoses
Reactome : Myoclonic epilepsy of Lafora

Protein

UniProt : Q9P2W7
HPRD : 06969

Clone

Cloning Information : 196169
HIP Master Clone ID : 170880
Original Clone ID : FLH196169.01L

Organism


TAX_ID : 9606
Species Specific ID: 27087

Genome

Chromosome : 11
Map Location : 11q25
Ensembl : ENSG00000109956

Reagents

Labome : CD57-antibody