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PTPRO (Homo sapiens) in pSGX3 (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PTPRO
Gene Name:  protein tyrosine phosphatase, receptor type, O
Sequence             
Original Clone ID: 8635a3BCt1p1
Keyword: full-length cds
Species: Homo sapiens
Type: cDNA
Vector Name: pSGX3               Format:  FUSION
Source: New York SGX Research Center for Structural Genomics
Description: None
Comments: Linker not verified.
Mutation/
Discrepancy :
No / No
Publications: PMID: 18058037
Title: Structural genomics of protein phosphatases
Authors: PSI
New York SGX Research Center for Structural Genomics
HsCD00423639

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No restriction
Special MTA: PSI

 

Insert sequence: 810nts         Open reading frame : 51 to 860

ATGACTTTGATGCCTATATTAAGGATATGGCCAAAGACTCTGACTATAAATTTTCTCTTC
AGTTTGAGGAGTTGAAATTGATTGGACTGGATATCCCACACTTTGCTGCAGATCTGCCAC
TGAATCGATGTAAAAACCGTTACACAAACATCCTACCATATGACTTCAGCCGTGTGAGAT
TAGTCTCCATGAATGAAGAGGAAGGTGCAGACTACATCAATGCCAACTATATTCCTGGAT
ACAACTCACCCCAGGAGTATATTGCCACCCAGGGGCCACTGCCTGAAACCAGAAATGACT
TCTGGAAGATGGTCCTGCAACAAAAGTCTCAGATTATTGTCATGCTCACTCAGTGTAATG
AGAAAAGGAGGGTGAAATGTGACCATTACTGGCCATTCACGGAAGAACCTATAGCCTATG
GAGACATCACTGTGGAGATGATTTCAGAGGAAGAGCAGGACGACTGGGCCTGTAGACACT
TCCGGATCAACTATGCTGACGAGATGCAGGATGTGATGCATTTTAACTACACTGCATGGC
CTGATCATGGTGTGCCCACAGCAAATGCTGCAGAAAGTATCCTGCAGTTTGTACACATGG
TCCGACAGCAAGCTACCAAGAGCAAAGGTCCCATGATCATTCACTGCAGTGCTGGCGTGG
GACGGACAGGAACATTCATTGCCCTGGACAGGCTCTTGCAGCACATTCGGGATCATGAGT
TTGTTGACATCTTAGGGCTGGTGTCAGAAATGAGGTCATACCGGATGTCTATGGTACAGA
CAGAGGAGCAGTACATTTTTATCCATCAGTGTGTGCAACTGATGTGGATGAAGAAGAAGG
AAGGGCATCATCACCATCAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGTCCCTT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-8635a
Recommended expression in: BL21(DE3)-Codon+RIL
       DyNA Vector Map

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Vector Name: pSGX3

pSGX3 in advanced viewed

Synonyms: pSGX-3, pSGX3_BC
Description: Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen
Size (bp): 5339
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: NYSGXRC
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ATG start start initiation start (Met, Ser, Leu) 5072 5079
TOPO recognition site TOPO TOPO cloning site 5076 5085
bacterial origin ori pBR322-type bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4983 5003
repressor protein gene LacI LacI CDS 3515 4531
selectable marker KanR kanamycin resistance gene 563 1375
tag His 6xHis tag 5086 5103
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 terminator 5231 5307

 


Cloning Information: 494273
GI: 13677214
GenBank Accession: NM_030667
HIP Master Clone ID: 407953
Labome: phosphotyrosine-antibody
Original Clone ID: 8635a3BCt1p1
PDB - Structural Annotation: 2G59
SMART domain: PTPc_motif : Protein tyrosine phosphatase, catalytic domain motif
SMART domain: PTPc : Protein tyrosine phosphatase, catalytic domain
Structural Annotation Wiki: TOPSAN
Structural Biology Knowledgebase: SBKB (2G59)
TargetTrack - Experimental data: NYSGXRC-8635a
Species Specific ID: 5800
Target GenBank: 454477
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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