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AF1035 (A. fulgidus DSM 4304 ) in pNYCOMPS-LIC-FH10T+ (N term)

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Explanation of Terms


Gene: Gene Symbol:  AF1035
Gene Name:  chemotaxis histidine kinase
Sequence             
Original Clone ID: GE.540.1.90_GO.13334
Keyword: None
Species: Archaeoglobus fulgidus DSM 4304
Type: cDNA
Vector Name: pNYCOMPS-LIC-FH10T+ (N term)               Format:  CLOSED
Source: New York Consortium on Membrane Protein Structure
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
New York Consortium on Membrane Protein Structure
AfCD00424412

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1290nts         Open reading frame : 49 to 1338

CATCACCATCACCACCATCACGAAAACCTGTATTTTCAATCCTACGTAATGCGGTTGCCG
GCAAAACTTAAAAAGAATGCTTTTGGATGGGAAATTGCACCTTTGAAATTATTGAAGATA
ATTACACTTTTGAAGATTAGGTTTATTTGCGCACTTTCCATTATTCTTCAAATGTTCAGA
GATTATGGACTTTTCAAAGCCCTTGCGGAAATGAGCCCTCATCCGATAATTTTACTTGAT
GGAACTGTAATTCGCTACGCAAATCCAAAGGCGTTGCAGCTTATTGGTGAAAACTGTCTG
CAAAGAGATATTCTCGATTTTCTGGATGAAAACAGCGGTAGATGTTTAAGGCAGGCACTT
CGGAAAGATCGCGGGACTTTTGAGGTGAGGTTCAGGGGCAAGGCAGGTGCATTGAAGGCT
GATTTGTTCAAACTTGACTCTAAAGCCGTGCTCACCTTAAGGTTTGACGTTGAGTCAGTT
TACAGGAAAATTTTCGAGACTGCACCTTCCCCCATAGCGGTTGTTGACTTAAATGGTGTT
GTGAGGGATGCGAACTTCTCCATGAAAGGATTTGCGGGATTCAACATCATCGGAAAAAAG
CTCAGTGAGCTTTTCTCTCAGGAAAGAGCGGAAAGGATAATGAGTTATGTTAATTTGGCT
TTGATGGAGAATACCGTGGTTGAGTTTGACGAAGAACTGGGAGACAGAAGGCTTGTAAGC
TACTGCATTCCTGCTGAGTTGGAGGGCGAAAGGCTTTGCTTTATCATTTCCCACGATGTA
ACCGAGTTTTACAGACTGAACAGGCTGCTTGAAAGGATTGTGGAGGCAAGCGAAGCGATG
ATTAAACTCAGAAGCAAGAGCAAGCTCATAGAAAGTGTTGAAAGCATACTCTCAGATTAC
TCTGCAAGGGTCCTCGACAGACCTGAGGGTGTTGCATTTAAAATTGAGTTCGATAGTATT
AGTTACGGCTACCTCTGCGTTAAAAATGCAGATTACGCTGAGAGACGACTACTCAAAACT
CTTGCGAAAAACCTGGCGTTCGCTCTAAAAGCGGTTGAGGATGAGCTGAGGAGGGAGGAG
TTCTTAAAGAGGCTGGTTGAAAACACCAAGGTAATCGCCTATCTCGTTGACAGAATCAGG
AACCCGCTAACGGCAATCAGGGCTTTTGCCGAAGTAATGATATCCGACGATAAAGTGAGG
GAGAGGATAACTCAGCAGGTGGACAGAATCGTGGGCATCATCAAGAACCTTGATTCGTCG
TGGGCGAAGTCCGAGGAAATTGTTGGGAAGGTTGACGTTGCGGACCTTCTCTATTCCGAT
AGCGGATTTCAAAACTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Tested_Not_Found
ProteinPurified: Tested_Not_Purified
SolubleProtein: Not_Applicable
Find experimental protocols in GO.13334
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pNYCOMPS-LIC-FH10T+ (N term)

pNYCOMPS-LIC-FH10TNterm in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector; T7 promoter; N terminal Flag epitope and 10X His tags; tags can be removed by TEV protease; kanamycin resistance; ligation independent cloning.
Comments: None
Size (bp): 6984
Parent Vector: None
Empty Vector: EvNO00091443
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: New York Consortium on Membrane Protein Structure
New York Structural Biology Center
Publications: PMID: 20394823
Title: High-throughput expression and purification of membrane proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 1583 2265
death cassette ccdB ccdB death cassette (removed in plasmids containing an insert) 5393 5602
promoter T7 T7 promoter 5146 5165
protease cleavage site TEV TEV protease cleavage site 5290 5310
repressor protein gene LacI Lac inhibitor 3677 4693
ribosome binding site RBS Ribosome Binding Site 5225 5230
selectable marker KanR kanamycin resistance 725 1537
selectable marker CmR Chloramphenicol resistance gene 6040 6696
tag His 10xHis tag; N terminal 5260 5289
tag Flag Flag epitope tag; N terminal 5236 5259
trxn regulatory element lac operator lac operator 5165 5187
trxn termination sequence T7 term T7 terminator 91 137

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Reagents

Gene

 

Gene Symbol : AF1035
GENEID : 1484258
Locus Tag : AF1035
GI : 11498640
Target GenBank: None

 

Protein

SMART domain : HisKA : His Kinase A (phosphoacceptor) domain
SMART domain : PAS : PAS domain
Structural Annotation Wiki : TOPSAN

Clone

Cloning Information : 554707
HIP Master Clone ID : 465027
Original Clone ID : GE.540.1.90_GO.13334

Experimental

TargetTrack - Experimental data : GO.13334

Organism


TAX_ID : 224325
Species Specific ID: 1484258

Reagents

Labome : AF1035-cDNA