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EIF2A (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  EIF2A
Gene Name:  eukaryotic translation initiation factor 2A, 65kDa
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH199407.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00042474

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1758nts         Open reading frame : 1 to 1758
Reference Sequence Alignment

ATGGCGCCGTCCACGCCGCTCTTGACAGTCCGAGGATCAGAAGGACTGTACATGGTGAAT
GGACCACCACATTTTACAGAAAGCACAGTGTTTCCAAGGGAATCTGGGAAGAATTGCAAA
GTCTGTATCTTTAGTAAGGATGGGACCTTGTTTGCCTGGGGCAATGGAGAAAAAGTAAAT
ATTATCAGTGTCACTAACAAGGGACTACTGCACTCCTTCGACCTCCTGAAGGCAGTTTGC
CTTGAATTCTCACCCAAAAATACTGTCCTGGCAACGTGGCAGCCTTACACTACTTCTAAA
GATGGCACAGCTGGGATACCCAACCTACAACTTTATGATGTGAAAACTGGGACATGTTTG
AAATCTTTCATCCAGAAAAAAATGCAAAATTGGTGTCCATCCTGGTCAGAAGATGAAACT
CTTTGTGCCCGCAATGTTAACAATGAAGTTCACTTCTTTGAAAACAACAATTTTAACACA
ATTGCAAATAAATTGCATTTGCAAAAAATTAATGACTTTGTATTATCACCTGGACCCCAA
CCATACAAGGTGGCTGTCTATGTTCCAGGAAGTAAAGGTGCACCTTCATTTGTTAGATTA
TATCAGTACCCCAACTTTGCTGGACCTCATGCAGCTTTAGCTAATAAAAGTTTCTTTAAG
GCAGATAAAGTTACAATGCTGTGGAATAAAAAAGCTACTGCTGTGTTGGTAATAGCTAGC
ACAGATGTTGACAAGACAGGAGCTTCCTACTATGGAGAACAAACTCTACACTACATTGCA
ACAAATGGAGAAAGTGCTGTAGTGCAATTACCAAAAAATGGCCCCATTTATGATGTAGTT
TGGAATTCTAGTTCTACTGAGTTTTGTGCTGTATATGGTTTTATGCCTGCCAAAGCGACA
ATTTTCAACTTGAAATGTGATCCTGTATTTGACTTTGGAACTGGTCCTCGTAATGCAGCC
TACTATAGCCCTCATGGACATATATTAGTATTAGCTGGATTTGGAAATCTGAGGGGACAA
ATGGAAGTGTGGGATGTGAAAAACTACAAACTTATTTCTAAACCGGTGGCTTCTGATTCT
ACATATTTTGCTTGGTGCCCGGATGGTGAGCATATTTTAACAGCTACATGTGCTCCCAGG
TTACGGGTTAATAATGGATACAAAATTTGGCATTATACTGGCTCTATCTTGCACAAGTAT
GATGTGCCATCAAATGCAGAATTATGGCAGGTTTCTTGGCAGCCATTTTTGGATGGAATA
TTTCCAGCAAAAACAATAACTTACCAAGCAGTTCCAAGTGAAGTACCCAATGAGGAACCT
AAAGTTGCAACAGCTTATAGACCCCCAGCTTTAAGAAATAAACCAATCACCAATTCCAAA
TTGCATGAAGAGGAACCACCTCAGAATATGAAACCACAATCAGGAAACGATAAGCCATTA
TCAAAAACAGCTCTTAAAAATCAAAGGAAGCATGAAGCTAAGAAAGCTGCAAAGCAGGAA
GCAAGAAGTGACAAGAGTCCAGATTTGGCACCTACTCCTGCCCCACAGAGCACACCACGA
AACACTGTCTCTCAGTCAATTTCTGGGGACCCTGAGATAGACAAAAAAATCAAGAACCTA
AAGAAGAAACTGAAAGCAATCGAACAACTGAAAGAACAAGCAGCAACTGGAAAACAGCTA
GAAAAAAATCAGTTGGAGAAAATTCAGAAAGAAACAGCCCTTCTCCAGGAGCTGGAAGAT
TTGAAATTGGGTATTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1764
Start on reference sequence 7
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : EIF2A
Symbol Nomenclature : EIF2A
SYNONYM : EIF-2A; MST089; MSTP004; MSTP089
Designation : 65 kDa eukaryotic translation initiation factor 2A|eukaryotic translation initiation factor 2A
Full Nomenclature : eukaryotic translation initiation factor 2A, 65kDa
GENEID : 83939
Locus Tag : CDA02
HGNC : 3254
MIM : 609234
Vega : OTTHUMG00000159775
Target GenBank: BC011885

 

Reference Sequence Alignment

  

HsCD00042474      ATGGCGCCGTCCACGCCGCTCTTGACAGTCCGAGGATCAGAAGGACTGTACATGGTGAAT
NM_032025.3       ATGGCGCCGTCCACGCCGCTCTTGACAGTCCGAGGATCAGAAGGACTGTACATGGTGAAT
                  ************************************************************

HsCD00042474      GGACCACCACATTTTACAGAAAGCACAGTGTTTCCAAGGGAATCTGGGAAGAATTGCAAA
NM_032025.3       GGACCACCACATTTTACAGAAAGCACAGTGTTTCCAAGGGAATCTGGGAAGAATTGCAAA
                  ************************************************************

HsCD00042474      GTCTGTATCTTTAGTAAGGATGGGACCTTGTTTGCCTGGGGCAATGGAGAAAAAGTAAAT
NM_032025.3       GTCTGTATCTTTAGTAAGGATGGGACCTTGTTTGCCTGGGGCAATGGAGAAAAAGTAAAT
                  ************************************************************

HsCD00042474      ATTATCAGTGTCACTAACAAGGGACTACTGCACTCCTTCGACCTCCTGAAGGCAGTTTGC
NM_032025.3       ATTATCAGTGTCACTAACAAGGGACTACTGCACTCCTTCGACCTCCTGAAGGCAGTTTGC
                  ************************************************************

HsCD00042474      CTTGAATTCTCACCCAAAAATACTGTCCTGGCAACGTGGCAGCCTTACACTACTTCTAAA
NM_032025.3       CTTGAATTCTCACCCAAAAATACTGTCCTGGCAACGTGGCAGCCTTACACTACTTCTAAA
                  ************************************************************

HsCD00042474      GATGGCACAGCTGGGATACCCAACCTACAACTTTATGATGTGAAAACTGGGACATGTTTG
NM_032025.3       GATGGCACAGCTGGGATACCCAACCTACAACTTTATGATGTGAAAACTGGGACATGTTTG
                  ************************************************************

HsCD00042474      AAATCTTTCATCCAGAAAAAAATGCAAAATTGGTGTCCATCCTGGTCAGAAGATGAAACT
NM_032025.3       AAATCTTTCATCCAGAAAAAAATGCAAAATTGGTGTCCATCCTGGTCAGAAGATGAAACT
                  ************************************************************

HsCD00042474      CTTTGTGCCCGCAATGTTAACAATGAAGTTCACTTCTTTGAAAACAACAATTTTAACACA
NM_032025.3       CTTTGTGCCCGCAATGTTAACAATGAAGTTCACTTCTTTGAAAACAACAATTTTAACACA
                  ************************************************************

HsCD00042474      ATTGCAAATAAATTGCATTTGCAAAAAATTAATGACTTTGTATTATCACCTGGACCCCAA
NM_032025.3       ATTGCAAATAAATTGCATTTGCAAAAAATTAATGATTTTGTATTATCACCTGGACCCCAA
                  *********************************** ************************

HsCD00042474      CCATACAAGGTGGCTGTCTATGTTCCAGGAAGTAAAGGTGCACCTTCATTTGTTAGATTA
NM_032025.3       CCATACAAGGTGGCTGTCTATGTTCCAGGAAGTAAAGGTGCACCTTCATTTGTTAGATTA
                  ************************************************************

HsCD00042474      TATCAGTACCCCAACTTTGCTGGACCTCATGCAGCTTTAGCTAATAAAAGTTTCTTTAAG
NM_032025.3       TATCAGTACCCCAACTTTGCTGGACCTCATGCAGCTTTAGCTAATAAAAGTTTCTTTAAG
                  ************************************************************

HsCD00042474      GCAGATAAAGTTACAATGCTGTGGAATAAAAAAGCTACTGCTGTGTTGGTAATAGCTAGC
NM_032025.3       GCAGATAAAGTTACAATGCTGTGGAATAAAAAAGCTACTGCTGTGTTGGTAATAGCTAGC
                  ************************************************************

HsCD00042474      ACAGATGTTGACAAGACAGGAGCTTCCTACTATGGAGAACAAACTCTACACTACATTGCA
NM_032025.3       ACAGATGTTGACAAGACAGGAGCTTCCTACTATGGAGAACAAACTCTACACTACATTGCA
                  ************************************************************

HsCD00042474      ACAAATGGAGAAAGTGCTGTAGTGCAATTACCAAAAAATGGCCCCATTTATGATGTAGTT
NM_032025.3       ACAAATGGAGAAAGTGCTGTAGTGCAATTACCAAAAAATGGCCCCATTTATGATGTAGTT
                  ************************************************************

HsCD00042474      TGGAATTCTAGTTCTACTGAGTTTTGTGCTGTATATGGTTTTATGCCTGCCAAAGCGACA
NM_032025.3       TGGAATTCTAGTTCTACTGAGTTTTGTGCTGTATATGGTTTTATGCCTGCCAAAGCGACA
                  ************************************************************

HsCD00042474      ATTTTCAACTTGAAATGTGATCCTGTATTTGACTTTGGAACTGGTCCTCGTAATGCAGCC
NM_032025.3       ATTTTCAACTTGAAATGTGATCCTGTATTTGACTTTGGAACTGGTCCTCGTAATGCAGCC
                  ************************************************************

HsCD00042474      TACTATAGCCCTCATGGACATATATTAGTATTAGCTGGATTTGGAAATCTGAGGGGACAA
NM_032025.3       TACTATAGCCCTCATGGACATATATTAGTATTAGCTGGATTTGGAAATCTGAGGGGACAA
                  ************************************************************

HsCD00042474      ATGGAAGTGTGGGATGTGAAAAACTACAAACTTATTTCTAAACCGGTGGCTTCTGATTCT
NM_032025.3       ATGGAAGTGTGGGATGTGAAAAACTACAAACTTATTTCTAAACCGGTGGCTTCTGATTCT
                  ************************************************************

HsCD00042474      ACATATTTTGCTTGGTGCCCGGATGGTGAGCATATTTTAACAGCTACATGTGCTCCCAGG
NM_032025.3       ACATATTTTGCTTGGTGCCCGGATGGTGAGCATATTTTAACAGCTACATGTGCTCCCAGG
                  ************************************************************

HsCD00042474      TTACGGGTTAATAATGGATACAAAATTTGGCATTATACTGGCTCTATCTTGCACAAGTAT
NM_032025.3       TTACGGGTTAATAATGGATACAAAATTTGGCATTATACTGGCTCTATCTTGCACAAGTAT
                  ************************************************************

HsCD00042474      GATGTGCCATCAAATGCAGAATTATGGCAGGTTTCTTGGCAGCCATTTTTGGATGGAATA
NM_032025.3       GATGTGCCATCAAATGCAGAATTATGGCAGGTTTCTTGGCAGCCATTTTTGGATGGAATA
                  ************************************************************

HsCD00042474      TTTCCAGCAAAAACAATAACTTACCAAGCAGTTCCAAGTGAAGTACCCAATGAGGAACCT
NM_032025.3       TTTCCAGCAAAAACAATAACTTACCAAGCAGTTCCAAGTGAAGTACCCAATGAGGAACCT
                  ************************************************************

HsCD00042474      AAAGTTGCAACAGCTTATAGACCCCCAGCTTTAAGAAATAAACCAATCACCAATTCCAAA
NM_032025.3       AAAGTTGCAACAGCTTATAGACCCCCAGCTTTAAGAAATAAACCAATCACCAATTCCAAA
                  ************************************************************

HsCD00042474      TTGCATGAAGAGGAACCACCTCAGAATATGAAACCACAATCAGGAAACGATAAGCCATTA
NM_032025.3       TTGCATGAAGAGGAACCACCTCAGAATATGAAACCACAATCAGGAAACGATAAGCCATTA
                  ************************************************************

HsCD00042474      TCAAAAACAGCTCTTAAAAATCAAAGGAAGCATGAAGCTAAGAAAGCTGCAAAGCAGGAA
NM_032025.3       TCAAAAACAGCTCTTAAAAATCAAAGGAAGCATGAAGCTAAGAAAGCTGCAAAGCAGGAA
                  ************************************************************

HsCD00042474      GCAAGAAGTGACAAGAGTCCAGATTTGGCACCTACTCCTGCCCCACAGAGCACACCACGA
NM_032025.3       GCAAGAAGTGACAAGAGTCCAGATTTGGCACCTACTCCTGCCCCACAGAGCACACCACGA
                  ************************************************************

HsCD00042474      AACACTGTCTCTCAGTCAATTTCTGGGGACCCTGAGATAGACAAAAAAATCAAGAACCTA
NM_032025.3       AACACTGTCTCTCAGTCAATTTCTGGGGACCCTGAGATAGACAAAAAAATCAAGAACCTA
                  ************************************************************

HsCD00042474      AAGAAGAAACTGAAAGCAATCGAACAACTGAAAGAACAAGCAGCAACTGGAAAACAGCTA
NM_032025.3       AAGAAGAAACTGAAAGCAATCGAACAACTGAAAGAACAAGCAGCAACTGGAAAACAGCTA
                  ************************************************************

HsCD00042474      GAAAAAAATCAGTTGGAGAAAATTCAGAAAGAAACAGCCCTTCTCCAGGAGCTGGAAGAT
NM_032025.3       GAAAAAAATCAGTTGGAGAAAATTCAGAAAGAAACAGCCCTTCTCCAGGAGCTGGAAGAT
                  ************************************************************

HsCD00042474      TTGAAATTGGGTATTTTG
NM_032025.3       TTGGAATTGGGTATTTAA
                  ***.************:.

Pathways

NCI : Validated targets of C-MYC transcriptional activation
Panther : Apoptosis signaling pathway
Reactome : 3' -UTR-mediated translational regulation
Reactome : Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S
Reactome : Cap-dependent Translation Initiation
Reactome : Eukaryotic Translation Initiation
Reactome : Formation of the ternary complex, and subsequently, the 43S complex
Reactome : GTP hydrolysis and joining of the 60S ribosomal subunit
Reactome : Gene Expression
Reactome : L13a-mediated translational silencing of Ceruloplasmin expression
Reactome : Metabolism of proteins
Reactome : PERK regulates gene expression
Reactome : Recycling of eIF2:GDP
Reactome : Ribosomal scanning and start codon recognition
Reactome : Translation
Reactome : Translation initiation complex formation
Reactome : Unfolded Protein Response (UPR)
WikiPathway : Translation Factors

Protein

UniProt : P05198
UniProt : Q53XC0
HPRD : 11739

Clone

Cloning Information : 199407
HIP Master Clone ID : 174118
Original Clone ID : FLH199407.01L

Organism


TAX_ID : 9606
Species Specific ID: 1965

Genome

Chromosome : 3
Map Location : 3q25.1
Ensembl : ENSG00000144895

Reagents

Labome : EIF2A-antibody