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CKM (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CKM
Gene Name:  creatine kinase, muscle
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH000080.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors: HIP
HsCD00004260

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1146nts         Open reading frame : 1 to 1146
Reference Sequence Alignment

ATGCCATTCGGTAACACCCACAACAAGTTCAAGCTGAATTACAAGCCTGAGGAGGAGTAC
CCCGACCTCAGCAAACATAACAACCACATGGCCAAGGTACTGACCCTTGAACTCTACAAG
AAGCTGCGGGACAAGGAGACTCCATCTGGCTTCACTGTAGACGATGTCATCCAGACAGGA
GTGGACAACCCAGGTCACCCCTTCATCATGACCGTGGGCTGCGTGGCTGGTGATGAGGAG
TCCTACGAAGTTTTCAAGGAACTCTTTGACCCCATCATCTCGGATCGCCACGGGGGCTAC
AAACCCACTGACAAGCACAAGACTGACCTCAACCATGAAAACCTCAAGGGTGGAGACGAC
CTGGACCCCAACTACGTGCTCAGCAGCCGCGTCCGCACTGGCCGCAGCATCAAGGGCTAC
ACGTTGCCCCCACACTGCTCCCGTGGCGAGCGCCGGGCGGTGGAGAAGCTCTCTGTGGAA
GCTCTCAACAGCCTGACGGGCGAGTTCAAAGGGAAGTACTACCCTCTGAAGAGCATGACG
GAGAAGGAGCAGCAGCAGCTCATCGATGACCACTTCCTGTTCGACAAGCCCGTGTCCCCG
CTGCTGCTGGCCTCAGGCATGGCCCGCGACTGGCCCGACGCCCGTGGCATCTGGCACAAT
GACAACAAGAGCCTCCTGGTGTGGGTGAACGAGGAGGATCACCTCCGGGTCATCTCCATG
GAGAAGGGGGGCAACATGAAGGAGGTTTTCCGCCGCTTCTGCGTAGGGCTGCAGAAGATT
GAGGAGATCTTTAAGAAAGCTGGCCACCCCTTCATGTGGAACCAGCACCTGGGCTACGTG
CTCACCTGCCCATCCAACCTGGGCACTGGGCTGCGTGGAGGCGTGCATGTGAAGCTGGCG
CACCTGAGCAAGCACCCCAAGTTCGAGGAGATCCTCACCCGCCTGCGTCTGCAGAAGAGG
GGTACAGGTGGCGTGGACACAGCTGCCGTGGGCTCAGTATTTGACGTGTCCAACGCTGAT
CGGCTGGGCTCGTCCGAAGTAGAACAGGTGCAGCTGGTGGTGGATGGTGTGAAGCTCATG
GTGGAAATGGAGAAGAAGTTGGAGAAAGGCCAGTCCATCGACGACATGATCCCCGCCCAG
AAGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1219
Start on reference sequence 74
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CKM
Symbol Nomenclature : CKM
SYNONYM : CKMM; M-CK
Designation : creatine kinase M chain|creatine kinase M-type
Full Nomenclature : creatine kinase, muscle
GENEID : 1158
GI : 33303744
GenBank Accession : AY335559
HGNC : 1994
MIM : 123310
Vega : OTTHUMG00000181782
Target GenBank: BC007462

 

Reference Sequence Alignment

  

HsCD00004260        ------------------------------------------------------------
XM_005258497.1      ATGCCTGGGTTCAGGGTGGGTATGGTGCGGGAGCAGGGAGGTGAGAGGCTCAGCTGCCCT
NM_001824.4         ------------------------------------------------------------
                                                                                

HsCD00004260        ------------------------------------------------------------
XM_005258497.1      CCAGAACTCCTCCCTGGGGACAACCCCTCCCAGCCAATAGCACAGCCTAGGTCCCCCTAT
NM_001824.4         ------------------------------------------------------------
                                                                                

HsCD00004260        ------------------------------------------------------------
XM_005258497.1      ATAAGGCCACGGCTGCTGGCCCTTCCTTTGGGTCAGTGTCACCTCCAGGATACAGACAGC
NM_001824.4         ------------------------------------------------------------
                                                                                

HsCD00004260        ------------------------------------------ATGCCATTCGGTAACACC
XM_005258497.1      CCCCCTTCAGCCCAGCCCAGCCAGGTCTCCTACACCGCCACCATGCCATTCGGTAACACC
NM_001824.4         ------------------------------------------ATGCCATTCGGTAACACC
                                                              ******************

HsCD00004260        CACAACAAGTTCAAGCTGAATTACAAGCCTGAGGAGGAGTACCCCGACCTCAGCAAACAT
XM_005258497.1      CACAACAAGTTCAAGCTGAATTACAAGCCTGAGGAGGAGTACCCCGACCTCAGCAAACAT
NM_001824.4         CACAACAAGTTCAAGCTGAATTACAAGCCTGAGGAGGAGTACCCCGACCTCAGCAAACAT
                    ************************************************************

HsCD00004260        AACAACCACATGGCCAAGGTACTGACCCTTGAACTCTACAAGAAGCTGCGGGACAAGGAG
XM_005258497.1      AACAACCACATGGCCAAGGTACTGACCCTTGAACTCTACAAGAAGCTGCGGGACAAGGAG
NM_001824.4         AACAACCACATGGCCAAGGTACTGACCCTTGAACTCTACAAGAAGCTGCGGGACAAGGAG
                    ************************************************************

HsCD00004260        ACTCCATCTGGCTTCACTGTAGACGATGTCATCCAGACAGGAGTGGACAACCCAGGTCAC
XM_005258497.1      ACTCCATCTGGCTTCACTGTAGACGATGTCATCCAGACAGGAGTGGACAACCCAGGTCAC
NM_001824.4         ACTCCATCTGGCTTCACTGTAGACGATGTCATCCAGACAGGAGTGGACAACCCAGGTCAC
                    ************************************************************

HsCD00004260        CCCTTCATCATGACCGTGGGCTGCGTGGCTGGTGATGAGGAGTCCTACGAAGTTTTCAAG
XM_005258497.1      CCCTTCATCATGACCGTGGGCTGCGTGGCTGGTGATGAGGAGTCCTACGAAGTTTTCAAG
NM_001824.4         CCCTTCATCATGACCGTGGGCTGCGTGGCTGGTGATGAGGAGTCCTACGAAGTTTTCAAG
                    ************************************************************

HsCD00004260        GAACTCTTTGACCCCATCATCTCGGATCGCCACGGGGGCTACAAACCCACTGACAAGCAC
XM_005258497.1      GAACTCTTTGACCCCATCATCTCGGATCGCCACGGGGGCTACAAACCCACTGACAAGCAC
NM_001824.4         GAACTCTTTGACCCCATCATCTCGGATCGCCACGGGGGCTACAAACCCACTGACAAGCAC
                    ************************************************************

HsCD00004260        AAGACTGACCTCAACCATGAAAACCTCAAGGGTGGAGACGACCTGGACCCCAACTACGTG
XM_005258497.1      AAGACTGACCTCAACCATGAAAACCTCAAGGGTGGAGACGACCTGGACCCTAACTACGTG
NM_001824.4         AAGACTGACCTCAACCATGAAAACCTCAAGGGTGGAGACGACCTGGACCCTAACTACGTG
                    ************************************************** *********

HsCD00004260        CTCAGCAGCCGCGTCCGCACTGGCCGCAGCATCAAGGGCTACACGTTGCCCCCACACTGC
XM_005258497.1      CTCAGCAGCCGCGTCCGCACTGGCCGCAGCATCAAGGGCTACACGTTGCCCCCACACTGC
NM_001824.4         CTCAGCAGCCGCGTCCGCACTGGCCGCAGCATCAAGGGCTACACGTTGCCCCCACACTGC
                    ************************************************************

HsCD00004260        TCCCGTGGCGAGCGCCGGGCGGTGGAGAAGCTCTCTGTGGAAGCTCTCAACAGCCTGACG
XM_005258497.1      TCCCGTGGCGAGCGCCGGGCGGTGGAGAAGCTCTCTGTGGAAGCTCTCAACAGCCTGACG
NM_001824.4         TCCCGTGGCGAGCGCCGGGCGGTGGAGAAGCTCTCTGTGGAAGCTCTCAACAGCCTGACG
                    ************************************************************

HsCD00004260        GGCGAGTTCAAAGGGAAGTACTACCCTCTGAAGAGCATGACGGAGAAGGAGCAGCAGCAG
XM_005258497.1      GGCGAGTTCAAAGGGAAGTACTACCCTCTGAAGAGCATGACGGAGAAGGAGCAGCAGCAG
NM_001824.4         GGCGAGTTCAAAGGGAAGTACTACCCTCTGAAGAGCATGACGGAGAAGGAGCAGCAGCAG
                    ************************************************************

HsCD00004260        CTCATCGATGACCACTTCCTGTTCGACAAGCCCGTGTCCCCGCTGCTGCTGGCCTCAGGC
XM_005258497.1      CTCATCGATGACCACTTCCTGTTCGACAAGCCCGTGTCCCCGCTGCTGCTGGCCTCAGGC
NM_001824.4         CTCATCGATGACCACTTCCTGTTCGACAAGCCCGTGTCCCCGCTGCTGCTGGCCTCAGGC
                    ************************************************************

HsCD00004260        ATGGCCCGCGACTGGCCCGACGCCCGTGGCATCTGGCACAATGACAACAAGAGCCTCCTG
XM_005258497.1      ATGGCCCGCGACTGGCCCGACGCCCGTGGCATCTGGCACAATGACAACAAGAGCTTCCTG
NM_001824.4         ATGGCCCGCGACTGGCCCGACGCCCGTGGCATCTGGCACAATGACAACAAGAGCTTCCTG
                    ****************************************************** *****

HsCD00004260        GTGTGGGTGAACGAGGAGGATCACCTCCGGGTCATCTCCATGGAGAAGGGGGGCAACATG
XM_005258497.1      GTGTGGGTGAACGAGGAGGATCACCTCCGGGTCATCTCCATGGAGAAGGGGGGCAACATG
NM_001824.4         GTGTGGGTGAACGAGGAGGATCACCTCCGGGTCATCTCCATGGAGAAGGGGGGCAACATG
                    ************************************************************

HsCD00004260        AAGGAGGTTTTCCGCCGCTTCTGCGTAGGGCTGCAGAAGATTGAGGAGATCTTTAAGAAA
XM_005258497.1      AAGGAGGTTTTCCGCCGCTTCTGCGTAGGGCTGCAGAAGATTGAGGAGATCTTTAAGAAA
NM_001824.4         AAGGAGGTTTTCCGCCGCTTCTGCGTAGGGCTGCAGAAGATTGAGGAGATCTTTAAGAAA
                    ************************************************************

HsCD00004260        GCTGGCCACCCCTTCATGTGGAACCAGCACCTGGGCTACGTGCTCACCTGCCCATCCAAC
XM_005258497.1      GCTGGCCACCCCTTCATGTGGAACCAGCACCTGGGCTACGTGCTCACCTGCCCATCCAAC
NM_001824.4         GCTGGCCACCCCTTCATGTGGAACCAGCACCTGGGCTACGTGCTCACCTGCCCATCCAAC
                    ************************************************************

HsCD00004260        CTGGGCACTGGGCTGCGTGGAGGCGTGCATGTGAAGCTGGCGCACCTGAGCAAGCACCCC
XM_005258497.1      CTGGGCACTGGGCTGCGTGGAGGCGTGCATGTGAAGCTGGCGCACCTGAGCAAGCACCCC
NM_001824.4         CTGGGCACTGGGCTGCGTGGAGGCGTGCATGTGAAGCTGGCGCACCTGAGCAAGCACCCC
                    ************************************************************

HsCD00004260        AAGTTCGAGGAGATCCTCACCCGCCTGCGTCTGCAGAAGAGGGGTACAGGTGGCGTGGAC
XM_005258497.1      AAGTTCGAGGAGATCCTCACCCGCCTGCGTCTGCAGAAGAGGGGTACAGGTGGCGTGGAC
NM_001824.4         AAGTTCGAGGAGATCCTCACCCGCCTGCGTCTGCAGAAGAGGGGTACAGGTGGCGTGGAC
                    ************************************************************

HsCD00004260        ACAGCTGCCGTGGGCTCAGTATTTGACGTGTCCAACGCTGATCGGCTGGGCTCGTCCGAA
XM_005258497.1      ACAGCTGCCGTGGGCTCAGTATTTGACGTGTCCAACGCTGATCGGCTGGGCTCGTCCGAA
NM_001824.4         ACAGCTGCCGTGGGCTCAGTATTTGACGTGTCCAACGCTGATCGGCTGGGCTCGTCCGAA
                    ************************************************************

HsCD00004260        GTAGAACAGGTGCAGCTGGTGGTGGATGGTGTGAAGCTCATGGTGGAAATGGAGAAGAAG
XM_005258497.1      GTAGAACAGGTGCAGCTGGTGGTGGATGGTGTGAAGCTCATGGTGGAAATGGAGAAGAAG
NM_001824.4         GTAGAACAGGTGCAGCTGGTGGTGGATGGTGTGAAGCTCATGGTGGAAATGGAGAAGAAG
                    ************************************************************

HsCD00004260        TTGGAGAAAGGCCAGTCCATCGACGACATGATCCCCGCCCAGAAGTTG
XM_005258497.1      TTGGAGAAAGGCCAGTCCATTGACGACATGATCCCCGCCCAGAAGTAG
NM_001824.4         TTGGAGAAAGGCCAGTCCATTGACGACATGATCCCCGCCCAGAAGTAG
                    ******************** *************************:*

Pathways

NCI : Regulation of retinoblastoma protein
Reactome : Creatine metabolism
Reactome : Metabolism
Reactome : Metabolism of amino acids and derivatives
WikiPathway : Urea cycle and metabolism of amino groups

Protein

UniProt : B2R892
UniProt : P06732
HPRD : 00426

Clone

Cloning Information : 80
HIP Master Clone ID : 87288
Original Clone ID : FLH000080.01L

Organism


TAX_ID : 9606
Species Specific ID: 1158

Genome

Chromosome : 19
Map Location : 19q13.32
Ensembl : ENSG00000104879

Reagents

Labome : CKM-antibody