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GTF2F1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GTF2F1
Gene Name:  general transcription factor IIF, polypeptide 1, 74kDa
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH164338.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00042614

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1554nts         Open reading frame : 1 to 1554
Reference Sequence Alignment

ATGGCGGCCCTAGGCCCTAGCAGCCAGAATGTCACTGAATACGTCGTTCGAGTTCCTAAG
AATACAACCAAAAAATATAACATCATGGCTTTTAATGCAGCCGACAAAGTCAACTTTGCT
ACGTGGAATCAGGCTCGGCTGGAGCGGGACTTGAGCAACAAGAAAATCTACCAAGAGGAG
GAGATGCCCGAATCGGGCGCGGGCAGTGAGTTCAACCGCAAGCTTCGGGAGGAGGCTCGG
AGGAAGAAGTACGGCATCGTCCTCAAGGAGTTCCGGCCCGAGGACCAGCCCTGGCTGCTC
CGGGTCAACGGCAAATCAGGCAGGAAGTTCAAGGGCATCAAGAAGGGAGGCGTAACAGAG
AACACGTCCTACTACATCTTCACCCAGTGCCCCGACGGGGCCTTCGAGGCCTTCCCCGTG
CACAACTGGTACAATTTCACACCGCTGGCCCGGCATCGCACGCTCACTGCCGAGGAGGCC
GAGGAGGAGTGGGAGAGGAGGAACAAGGTGCTGAACCACTTCAGCATCATGCAGCAGCGG
CGGCTCAAGGATCAGGACCAGGACGAGGATGAGGAGGAGAAGGAGAAACGTGGCCGCAGG
AAGGCGAGCGAGCTGCGCATCCACGACCTGGAGGACGACCTGGAGATGTCGTCCGATGCC
AGTGATGCCAGTGGTGAGGAGGGGGGCAGAGTCCCCAAGGCCAAGAAGAAGGCGCCGCTG
GCCAAGGGCGGCAGGAAAAAGAAGAAGAAGAAGGGTTCAGACGACGAGGCCTTCGAGGAC
AGCGATGATGGGGACTTCGAGGGCCAAGAGGTGGACTACATGTCAGACGGCTCCAGTAGC
TCCCAAGAAGAGCCTGAGAGCAAGGCCAAGGCGCCGCAGCAGGAGGAGGGGCCCAAGGGT
GTCGATGAGCAGAGCGACAGTAGTGAGGAGAGTGAGGAGGAGAAGCCGCCTGAGGAGGAC
AAGGAGGAGGAGGAGGAGAAGAAGGCACCCACCCCGCAGGAGAAGAAGCGCAGGAAAGAC
AGCAGCGAGGAGTCGGACAGCTCAGAGGAGAGCGACATTGACAGCGAGGCCTCCTCAGCC
CTCTTCATGGCGAAGAAGAAGACGCCACCCAAGAGAGAGCGGAAGCCGTCGGGAGGGAGC
TCAAGGGGCAACAGCCGCCCAGGCACGCCCAGCGCAGAGGGTGGCAGCACCTCCTCCACC
CTGCGGGCGGCTGCCAGCAAACTCGAGCAAGGGAAGCGGGTGAGCGAGATGCCTGCAGCC
AAGCGGTTGCGGCTGGACACGGGACCCCAGAGCCTGTCTGGGAAGTCGACACCCCAGCCA
CCATCAGGCAAGACAACACCCAACAGCGGCGACGTGCAGGTGACTGAGGATGCCGTGCGC
CGCTACCTGACACGGAAGCCCATGACCACTAAGGACCTGCTGAAAAAGTTCCAGACCAAG
AAGACAGGGCTGAGCAGCGAGCAGACAGTGAACGTGTTGGCCCAGATCCTCAAGCGACTC
AACCCCGAGCGCAAGATGATCAACGACAAAATGCACTTCTCCCTCAAGGAGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1701
Start on reference sequence 148
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GTF2F1
Symbol Nomenclature : GTF2F1
SYNONYM : BTF4; RAP74; TF2F1; TFIIF
Designation : TFIIF-alpha|general transcription factor IIF 74 kDa subunit|general transcription factor IIF subunit 1|transcription initiation factor IIF subunit alpha|transcription initiation factor RAP74
Full Nomenclature : general transcription factor IIF, polypeptide 1, 74kDa
GENEID : 2962
HGNC : 4652
MIM : 189968
Vega : OTTHUMG00000168087
Target GenBank: BC000120

 

Reference Sequence Alignment

  

HsCD00042614      ATGGCGGCCCTAGGCCCTAGCAGCCAGAATGTCACTGAATACGTCGTTCGAGTTCCTAAG
NM_002096.2       ATGGCGGCCCTAGGCCCTAGCAGCCAGAATGTCACTGAATACGTCGTTCGAGTTCCTAAG
                  ************************************************************

HsCD00042614      AATACAACCAAAAAATATAACATCATGGCTTTTAATGCAGCCGACAAAGTCAACTTTGCT
NM_002096.2       AATACAACCAAAAAATATAACATCATGGCTTTTAATGCAGCCGACAAAGTCAACTTTGCT
                  ************************************************************

HsCD00042614      ACGTGGAATCAGGCTCGGCTGGAGCGGGACTTGAGCAACAAGAAAATCTACCAAGAGGAG
NM_002096.2       ACGTGGAATCAGGCTCGGCTGGAGCGGGACTTGAGCAACAAGAAAATCTACCAAGAGGAG
                  ************************************************************

HsCD00042614      GAGATGCCCGAATCGGGCGCGGGCAGTGAGTTCAACCGCAAGCTTCGGGAGGAGGCTCGG
NM_002096.2       GAGATGCCCGAATCGGGCGCGGGCAGTGAGTTCAACCGCAAGCTTCGGGAGGAGGCTCGG
                  ************************************************************

HsCD00042614      AGGAAGAAGTACGGCATCGTCCTCAAGGAGTTCCGGCCCGAGGACCAGCCCTGGCTGCTC
NM_002096.2       AGGAAGAAGTACGGCATCGTCCTCAAGGAGTTCCGGCCCGAGGACCAGCCCTGGCTGCTC
                  ************************************************************

HsCD00042614      CGGGTCAACGGCAAATCAGGCAGGAAGTTCAAGGGCATCAAGAAGGGAGGCGTAACAGAG
NM_002096.2       CGGGTCAACGGCAAATCAGGCAGGAAGTTCAAGGGCATCAAGAAGGGAGGCGTAACAGAG
                  ************************************************************

HsCD00042614      AACACGTCCTACTACATCTTCACCCAGTGCCCCGACGGGGCCTTCGAGGCCTTCCCCGTG
NM_002096.2       AACACGTCCTACTACATCTTCACCCAGTGCCCCGACGGGGCCTTCGAGGCCTTCCCCGTG
                  ************************************************************

HsCD00042614      CACAACTGGTACAATTTCACACCGCTGGCCCGGCATCGCACGCTCACTGCCGAGGAGGCC
NM_002096.2       CACAACTGGTACAATTTCACACCGCTGGCCCGGCATCGCACGCTCACTGCCGAGGAGGCC
                  ************************************************************

HsCD00042614      GAGGAGGAGTGGGAGAGGAGGAACAAGGTGCTGAACCACTTCAGCATCATGCAGCAGCGG
NM_002096.2       GAGGAGGAGTGGGAGAGGAGGAACAAGGTGCTGAACCACTTCAGCATCATGCAGCAGCGG
                  ************************************************************

HsCD00042614      CGGCTCAAGGATCAGGACCAGGACGAGGATGAGGAGGAGAAGGAGAAACGTGGCCGCAGG
NM_002096.2       CGGCTCAAGGATCAGGACCAGGACGAGGATGAGGAGGAGAAGGAGAAACGTGGCCGCAGG
                  ************************************************************

HsCD00042614      AAGGCGAGCGAGCTGCGCATCCACGACCTGGAGGACGACCTGGAGATGTCGTCCGATGCC
NM_002096.2       AAGGCGAGCGAGCTGCGCATCCACGACCTGGAGGACGACCTGGAGATGTCGTCCGATGCC
                  ************************************************************

HsCD00042614      AGTGATGCCAGTGGTGAGGAGGGGGGCAGAGTCCCCAAGGCCAAGAAGAAGGCGCCGCTG
NM_002096.2       AGTGATGCCAGTGGTGAGGAGGGGGGCAGAGTCCCCAAGGCCAAGAAGAAGGCGCCGCTG
                  ************************************************************

HsCD00042614      GCCAAGGGCGGCAGGAAAAAGAAGAAGAAGAAGGGTTCAGACGACGAGGCCTTCGAGGAC
NM_002096.2       GCCAAGGGCGGCAGGAAAAAGAAGAAGAAGAAGGGTTCAGACGACGAGGCCTTCGAGGAC
                  ************************************************************

HsCD00042614      AGCGATGATGGGGACTTCGAGGGCCAAGAGGTGGACTACATGTCAGACGGCTCCAGTAGC
NM_002096.2       AGCGATGATGGGGACTTCGAGGGCCAAGAGGTGGACTACATGTCAGACGGCTCCAGTAGC
                  ************************************************************

HsCD00042614      TCCCAAGAAGAGCCTGAGAGCAAGGCCAAGGCGCCGCAGCAGGAGGAGGGGCCCAAGGGT
NM_002096.2       TCCCAAGAAGAGCCTGAGAGCAAGGCCAAGGCGCCGCAGCAGGAGGAGGGGCCCAAGGGT
                  ************************************************************

HsCD00042614      GTCGATGAGCAGAGCGACAGTAGTGAGGAGAGTGAGGAGGAGAAGCCGCCTGAGGAGGAC
NM_002096.2       GTCGATGAGCAGAGCGACAGTAGTGAGGAGAGTGAGGAGGAGAAGCCGCCTGAGGAGGAC
                  ************************************************************

HsCD00042614      AAGGAGGAGGAGGAGGAGAAGAAGGCACCCACCCCGCAGGAGAAGAAGCGCAGGAAAGAC
NM_002096.2       AAGGAGGAGGAGGAGGAGAAGAAGGCACCCACCCCGCAGGAGAAGAAGCGCAGGAAAGAC
                  ************************************************************

HsCD00042614      AGCAGCGAGGAGTCGGACAGCTCAGAGGAGAGCGACATTGACAGCGAGGCCTCCTCAGCC
NM_002096.2       AGCAGCGAGGAGTCGGACAGCTCAGAGGAGAGCGACATTGACAGCGAGGCCTCCTCAGCC
                  ************************************************************

HsCD00042614      CTCTTCATGGCGAAGAAGAAGACGCCACCCAAGAGAGAGCGGAAGCCGTCGGGAGGGAGC
NM_002096.2       CTCTTCATGGCGAAGAAGAAGACGCCACCCAAGAGAGAGCGGAAGCCGTCGGGAGGGAGC
                  ************************************************************

HsCD00042614      TCAAGGGGCAACAGCCGCCCAGGCACGCCCAGCGCAGAGGGTGGCAGCACCTCCTCCACC
NM_002096.2       TCAAGGGGCAACAGCCGCCCAGGCACGCCCAGCGCAGAGGGTGGCAGCACCTCCTCCACC
                  ************************************************************

HsCD00042614      CTGCGGGCGGCTGCCAGCAAACTCGAGCAAGGGAAGCGGGTGAGCGAGATGCCTGCAGCC
NM_002096.2       CTGCGGGCGGCTGCCAGCAAACTCGAGCAAGGGAAGCGGGTGAGCGAGATGCCTGCAGCC
                  ************************************************************

HsCD00042614      AAGCGGTTGCGGCTGGACACGGGACCCCAGAGCCTGTCTGGGAAGTCGACACCCCAGCCA
NM_002096.2       AAGCGGTTGCGGCTGGACACGGGACCCCAGAGCCTGTCTGGGAAGTCGACACCCCAGCCA
                  ************************************************************

HsCD00042614      CCATCAGGCAAGACAACACCCAACAGCGGCGACGTGCAGGTGACTGAGGATGCCGTGCGC
NM_002096.2       CCATCAGGCAAGACAACACCCAACAGCGGCGACGTGCAGGTGACTGAGGATGCCGTGCGC
                  ************************************************************

HsCD00042614      CGCTACCTGACACGGAAGCCCATGACCACTAAGGACCTGCTGAAAAAGTTCCAGACCAAG
NM_002096.2       CGCTACCTGACACGGAAGCCCATGACCACTAAGGACCTGCTGAAAAAGTTCCAGACCAAG
                  ************************************************************

HsCD00042614      AAGACAGGGCTGAGCAGCGAGCAGACAGTGAACGTGTTGGCCCAGATCCTCAAGCGACTC
NM_002096.2       AAGACAGGGCTGAGCAGCGAGCAGACAGTGAACGTGTTGGCCCAGATCCTCAAGCGACTC
                  ************************************************************

HsCD00042614      AACCCCGAGCGCAAGATGATCAACGACAAAATGCACTTCTCCCTCAAGGAGTAG
NM_002096.2       AACCCCGAGCGCAAGATGATCAACGACAAAATGCACTTCTCCCTCAAGGAGTGA
                  ****************************************************..

Pathways

Panther : General transcription regulation
Panther : Transcription regulation by bZIP transcription factor
Reactome : Abortive elongation of HIV-1 transcript in the absence of Tat
Reactome : Disease
Reactome : Elongation arrest and recovery
Reactome : Formation of HIV elongation complex in the absence of HIV Tat
Reactome : Formation of HIV-1 elongation complex containing HIV-1 Tat
Reactome : Formation of RNA Pol II elongation complex
Reactome : Formation of the Early Elongation Complex
Reactome : Formation of the HIV-1 Early Elongation Complex
Reactome : Gene Expression
Reactome : HIV Infection
Reactome : HIV Life Cycle
Reactome : HIV Transcription Elongation
Reactome : HIV Transcription Initiation
Reactome : HIV elongation arrest and recovery
Reactome : Influenza Infection
Reactome : Influenza Life Cycle
Reactome : Influenza Viral RNA Transcription and Replication
Reactome : Late Phase of HIV Life Cycle
Reactome : Pausing and recovery of HIV elongation
Reactome : Pausing and recovery of Tat-mediated HIV elongation
Reactome : Processing of Capped Intron-Containing Pre-mRNA
Reactome : RNA Pol II CTD phosphorylation and interaction with CE
Reactome : RNA Polymerase II HIV Promoter Escape
Reactome : RNA Polymerase II Pre-transcription Events
Reactome : RNA Polymerase II Promoter Escape
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Elongation
Reactome : RNA Polymerase II Transcription Initiation
Reactome : RNA Polymerase II Transcription Initiation And Promoter Clearance
Reactome : RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
Reactome : Tat-mediated HIV elongation arrest and recovery
Reactome : Tat-mediated elongation of the HIV-1 transcript
Reactome : Transcription
Reactome : Transcription of the HIV genome
Reactome : Viral Messenger RNA Synthesis
Reactome : mRNA Capping
Reactome : mRNA Splicing
Reactome : mRNA Splicing - Major Pathway
Reactome : mRNA Splicing - Minor Pathway

Protein

UniProt : P35269
HPRD : 01803

Clone

Cloning Information : 164338
HIP Master Clone ID : 137538
Original Clone ID : FLH164338.01X

Organism


TAX_ID : 9606
Species Specific ID: 2962

Genome

Chromosome : 19
Map Location : 19p13.3
Ensembl : ENSG00000125651

Reagents

Labome : GTF2F1-antibody