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ARNT2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ARNT2
Gene Name:  aryl-hydrocarbon receptor nuclear translocator 2
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH168375.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00042627

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2151nts         Open reading frame : 1 to 2151
Reference Sequence Alignment

ATGGCAACCCCGGCGGCGGTCAACCCTCCGGAAATGGCTTCAGACATACCTGGATCTGTG
ACGTTGCCCGTTGCCCCCATGGCGGCCACCGGACAGGTGAGGATGGCGGGGGCCATGCCT
GCCCGTGGAGGAAAGCGGCGTTCCGGAATGGACTTCGATGATGAAGATGGTGAAGGCCCC
AGTAAATTTTCAAGAGAGAATCATAGTGAAATCGAAAGGCGCAGACGGAACAAGATGACT
CAGTACATCACGGAGCTCTCCGACATGGTCCCCACATGCAGCGCACTGGCTCGGAAGCCA
GACAAGCTCACCATCCTCCGCATGGCCGTCTCGCACATGAAGTCCATGAGGGGTACAGGG
AACAAGTCCACCGATGGCGCGTACAAGCCTTCCTTCCTCACAGAGCAGGAACTGAAGCAT
CTCATCCTTGAAGCAGCTGATGGATTTCTGTTTGTGGTGGCTGCTGAGACAGGGCGAGTG
ATTTATGTGTCTGACTCCGTCACCCCTGTTCTGAACCAGCCCCAGTCAGAGTGGTTTGGG
AGCACACTGTATGAACAGGTGCATCCTGATGACGTGGAGAAGCTGAGAGAGCAACTGTGC
ACCTCAGAAAACTCAATGACAGGCCGGATCTTGGACCTGAAGACTGGGACGGTCAAGAAA
GAAGGGCAGCAGTCATCCATGAGGATGTGCATGGGCTCGCGGCGGTCTTTCATCTGCAGG
ATGAGGTGTGGAAATGCTCCTTTGGACCACCTTCCTCTAAACAGAATAACCACCATGAGG
AAAAGGTTCAGGAATGGCCTTGGCCCTGTGAAAGAAGGAGAAGCCCAATATGCTGTGGTC
CACTGTACAGGATACATCAAGGCCTGGCCACCAGCAGGAATGACCATACCTGAAGAAGAC
GCTGATGTGGGACAAGGCAGTAAATATTGCCTCGTGGCAATTGGGAGACTCCAGGTGACC
AGCTCTCCTGTATGCATGGACATGAATGGGATGTCGGTGCCCACAGAGTTCTTATCCCGG
CATAACTCCGATGGAATCATCACATTTGTGGATCCAAGATGTATCAGTGTGATTGGCTAC
CAACCGCAGGATCTTCTGGGAAAGGACATTTTGGAATTCTGCCACCCTGAGGATCAAAGC
CATCTGCGTGAGAGCTTCCAGCAGGTGGTTAAGCTGAAAGGCCAAGTCCTGTCGGTCATG
TATCGATTTCGCACCAAGAACCGGGAGTGGATGTTGATCCGCACCAGCAGCTTCACATTC
CAGAATCCCTATTCTGATGAGATTGAGTACATCATCTGCACCAACACCAACGTCAAGCAA
CTTCAGCAACAGCAGGCAGAATTGGAAGTGCACCAGAGAGATGGATTGTCATCGTATGAC
TTATCCCAGGTCCCCGTCCCCAACCTACCAGCCGGTGTTCATGAGGCCGGGAAGTCCGTG
GAAAAGGCGGATGCAATCTTCTCCCAGGAAAGAGATCCTCGGTTTGCTGAAATGTTTGCA
GGAATTAGTGCATCGGAGAAGAAGATGATGAGCTCAGCCTCTGCAGCAGGAACCCAGCAG
ATCTACTCCCAAGGAAGCCCATTTCCCTCTGGACACTCCGGGAAGGCCTTCAGCTCTTCA
GTGGTTCATGTGCCTGGAGTGAATGATATTCAGTCCTCTTCTTCCACGGGCCAGAACATG
TCCCAAATCTCCCGGCAGCTAAACCAGAGTCAGGTGGCATGGACAGGGAGTCGTCCGCCC
TTTCCGGGACAGCAAATCCCATCTCAGTCCAGCAAGACTCAGTCATCTCCCTTTGGGATT
GGAACGAGCCACACCTACCCGGCAGACCCCTCTTCCTACAGCCCCCTCTCCAGCCCAGCT
ACCTCCTCGCCAAGTGGGAATGCCTACTCCAGTCTTGCCAACAGGACTCCAGGGTTCGCT
GAAAGTGGACAAAGTAGCGGGCAGTTCCAAGGGCGGCCCTCGGAAGTCTGGTCGCAGTGG
CAAAGCCAGCACCATGGCCAGCAGAGCGGTGAGCAGCACTCCCACCAGCAGCCCGGTCAG
ACTGAAGTGTTCCAGGACATGCTGCCCATGCCAGGAGATCCAGGGAATGAATGGTGGTCT
CCCCACTCCCGGCAGCACTTTAGGCAGCCCATAAGCTATGCGAGAATGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2266
Start on reference sequence 116
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ARNT2
Symbol Nomenclature : ARNT2
SYNONYM : bHLHe1
Designation : ARNT protein 2|aryl hydrocarbon receptor nuclear translocator 2|class E basic helix-loop-helix protein 1
Full Nomenclature : aryl-hydrocarbon receptor nuclear translocator 2
GENEID : 9915
HGNC : 16876
MIM : 606036
Vega : OTTHUMG00000165478
Target GenBank: BC036099

 

Reference Sequence Alignment

  

HsCD00042627      ATGGCAACCCCGGCGGCGGTCAACCCTCCGGAAATGGCTTCAGACATACCTGGATCTGTG
NM_014862.3       ATGGCAACCCCGGCGGCGGTCAACCCTCCGGAAATGGCTTCAGACATACCTGGATCTGTG
                  ************************************************************

HsCD00042627      ACGTTGCCCGTTGCCCCCATGGCGGCCACCGGACAGGTGAGGATGGCGGGGGCCATGCCT
NM_014862.3       ACGTTGCCCGTTGCCCCCATGGCGGCCACCGGACAGGTGAGGATGGCGGGGGCCATGCCT
                  ************************************************************

HsCD00042627      GCCCGTGGAGGAAAGCGGCGTTCCGGAATGGACTTCGATGATGAAGATGGTGAAGGCCCC
NM_014862.3       GCCCGTGGAGGAAAGCGGCGTTCCGGAATGGACTTCGATGATGAAGATGGTGAAGGCCCC
                  ************************************************************

HsCD00042627      AGTAAATTTTCAAGAGAGAATCATAGTGAAATCGAAAGGCGCAGACGGAACAAGATGACT
NM_014862.3       AGTAAATTTTCAAGAGAGAATCATAGTGAAATCGAAAGGCGCAGACGGAACAAGATGACT
                  ************************************************************

HsCD00042627      CAGTACATCACGGAGCTCTCCGACATGGTCCCCACATGCAGCGCACTGGCTCGGAAGCCA
NM_014862.3       CAGTACATCACGGAGCTCTCCGACATGGTCCCCACATGCAGCGCACTGGCTCGGAAGCCA
                  ************************************************************

HsCD00042627      GACAAGCTCACCATCCTCCGCATGGCCGTCTCGCACATGAAGTCCATGAGGGGTACAGGG
NM_014862.3       GACAAGCTCACCATCCTCCGCATGGCCGTCTCGCACATGAAGTCCATGAGGGGTACAGGG
                  ************************************************************

HsCD00042627      AACAAGTCCACCGATGGCGCGTACAAGCCTTCCTTCCTCACAGAGCAGGAACTGAAGCAT
NM_014862.3       AACAAGTCCACCGATGGCGCGTACAAGCCTTCCTTCCTCACAGAGCAGGAACTGAAGCAT
                  ************************************************************

HsCD00042627      CTCATCCTTGAAGCAGCTGATGGATTTCTGTTTGTGGTGGCTGCTGAGACAGGGCGAGTG
NM_014862.3       CTCATCCTTGAAGCAGCTGATGGATTTCTGTTTGTGGTGGCTGCTGAGACAGGGCGAGTG
                  ************************************************************

HsCD00042627      ATTTATGTGTCTGACTCCGTCACCCCTGTTCTGAACCAGCCCCAGTCAGAGTGGTTTGGG
NM_014862.3       ATTTATGTGTCTGACTCCGTCACCCCTGTTCTGAACCAGCCCCAGTCAGAGTGGTTTGGG
                  ************************************************************

HsCD00042627      AGCACACTGTATGAACAGGTGCATCCTGATGACGTGGAGAAGCTGAGAGAGCAACTGTGC
NM_014862.3       AGCACACTGTATGAACAGGTGCATCCTGATGACGTGGAGAAGCTGAGAGAGCAACTGTGC
                  ************************************************************

HsCD00042627      ACCTCAGAAAACTCAATGACAGGCCGGATCTTGGACCTGAAGACTGGGACGGTCAAGAAA
NM_014862.3       ACCTCAGAAAACTCAATGACAGGCCGGATCTTGGACCTGAAGACTGGGACGGTCAAGAAA
                  ************************************************************

HsCD00042627      GAAGGGCAGCAGTCATCCATGAGGATGTGCATGGGCTCGCGGCGGTCTTTCATCTGCAGG
NM_014862.3       GAAGGGCAGCAGTCATCCATGAGGATGTGCATGGGCTCGCGGCGGTCTTTCATCTGCAGG
                  ************************************************************

HsCD00042627      ATGAGGTGTGGAAATGCTCCTTTGGACCACCTTCCTCTAAACAGAATAACCACCATGAGG
NM_014862.3       ATGAGGTGTGGAAATGCTCCTTTGGACCACCTTCCTCTAAACAGAATAACCACCATGAGG
                  ************************************************************

HsCD00042627      AAAAGGTTCAGGAATGGCCTTGGCCCTGTGAAAGAAGGAGAAGCCCAATATGCTGTGGTC
NM_014862.3       AAAAGGTTCAGGAATGGCCTTGGCCCTGTGAAAGAAGGAGAAGCCCAATATGCTGTGGTC
                  ************************************************************

HsCD00042627      CACTGTACAGGATACATCAAGGCCTGGCCACCAGCAGGAATGACCATACCTGAAGAAGAC
NM_014862.3       CACTGTACAGGATACATCAAGGCCTGGCCACCAGCAGGAATGACCATACCTGAAGAAGAC
                  ************************************************************

HsCD00042627      GCTGATGTGGGACAAGGCAGTAAATATTGCCTCGTGGCAATTGGGAGACTCCAGGTGACC
NM_014862.3       GCTGATGTGGGACAAGGCAGTAAATATTGCCTCGTGGCAATTGGGAGACTCCAGGTGACC
                  ************************************************************

HsCD00042627      AGCTCTCCTGTATGCATGGACATGAATGGGATGTCGGTGCCCACAGAGTTCTTATCCCGG
NM_014862.3       AGCTCTCCTGTATGCATGGACATGAATGGGATGTCGGTGCCCACAGAGTTCTTATCCCGG
                  ************************************************************

HsCD00042627      CATAACTCCGATGGAATCATCACATTTGTGGATCCAAGATGTATCAGTGTGATTGGCTAC
NM_014862.3       CATAACTCCGATGGAATCATCACATTTGTGGATCCAAGATGTATCAGTGTGATTGGCTAC
                  ************************************************************

HsCD00042627      CAACCGCAGGATCTTCTGGGAAAGGACATTTTGGAATTCTGCCACCCTGAGGATCAAAGC
NM_014862.3       CAACCCCAGGATCTTCTGGGAAAGGACATTTTGGAATTCTGCCACCCTGAGGATCAAAGC
                  ***** ******************************************************

HsCD00042627      CATCTGCGTGAGAGCTTCCAGCAGGTGGTTAAGCTGAAAGGCCAAGTCCTGTCGGTCATG
NM_014862.3       CATCTGCGTGAGAGCTTCCAGCAGGTGGTTAAGCTGAAAGGCCAAGTCCTGTCGGTCATG
                  ************************************************************

HsCD00042627      TATCGATTTCGCACCAAGAACCGGGAGTGGATGTTGATCCGCACCAGCAGCTTCACATTC
NM_014862.3       TATCGATTTCGCACCAAGAACCGGGAGTGGATGTTGATCCGCACCAGCAGCTTCACATTC
                  ************************************************************

HsCD00042627      CAGAATCCCTATTCTGATGAGATTGAGTACATCATCTGCACCAACACCAACGTCAAGCAA
NM_014862.3       CAGAATCCCTATTCTGATGAGATTGAGTACATCATCTGCACCAACACCAACGTCAAGCAA
                  ************************************************************

HsCD00042627      CTTCAGCAACAGCAGGCAGAATTGGAAGTGCACCAGAGAGATGGATTGTCATCGTATGAC
NM_014862.3       CTTCAGCAACAGCAGGCAGAATTGGAAGTGCACCAGAGAGATGGATTGTCATCGTATGAC
                  ************************************************************

HsCD00042627      TTATCCCAGGTCCCCGTCCCCAACCTACCAGCCGGTGTTCATGAGGCCGGGAAGTCCGTG
NM_014862.3       TTATCCCAGGTCCCCGTCCCCAACCTACCAGCCGGTGTTCATGAGGCCGGGAAGTCCGTG
                  ************************************************************

HsCD00042627      GAAAAGGCGGATGCAATCTTCTCCCAGGAAAGAGATCCTCGGTTTGCTGAAATGTTTGCA
NM_014862.3       GAAAAGGCGGATGCAATCTTCTCCCAGGAAAGAGATCCTCGGTTTGCTGAAATGTTTGCA
                  ************************************************************

HsCD00042627      GGAATTAGTGCATCGGAGAAGAAGATGATGAGCTCAGCCTCTGCAGCAGGAACCCAGCAG
NM_014862.3       GGAATTAGTGCATCGGAGAAGAAGATGATGAGCTCAGCCTCTGCAGCAGGAACCCAGCAG
                  ************************************************************

HsCD00042627      ATCTACTCCCAAGGAAGCCCATTTCCCTCTGGACACTCCGGGAAGGCCTTCAGCTCTTCA
NM_014862.3       ATCTACTCCCAAGGAAGCCCATTTCCCTCTGGACACTCCGGGAAGGCCTTCAGCTCTTCA
                  ************************************************************

HsCD00042627      GTGGTTCATGTGCCTGGAGTGAATGATATTCAGTCCTCTTCTTCCACGGGCCAGAACATG
NM_014862.3       GTGGTTCATGTGCCTGGAGTGAATGATATTCAGTCCTCTTCTTCCACGGGCCAGAACATG
                  ************************************************************

HsCD00042627      TCCCAAATCTCCCGGCAGCTAAACCAGAGTCAGGTGGCATGGACAGGGAGTCGTCCGCCC
NM_014862.3       TCCCAAATCTCCCGGCAGCTAAACCAGAGTCAGGTGGCATGGACAGGGAGTCGTCCGCCC
                  ************************************************************

HsCD00042627      TTTCCGGGACAGCAAATCCCATCTCAGTCCAGCAAGACTCAGTCATCTCCCTTTGGGATT
NM_014862.3       TTTCCGGGACAGCAAATCCCATCTCAGTCCAGCAAGACTCAGTCATCTCCCTTTGGGATT
                  ************************************************************

HsCD00042627      GGAACGAGCCACACCTACCCGGCAGACCCCTCTTCCTACAGCCCCCTCTCCAGCCCAGCT
NM_014862.3       GGAACGAGCCACACCTACCCGGCAGACCCCTCTTCCTACAGCCCCCTCTCCAGCCCAGCT
                  ************************************************************

HsCD00042627      ACCTCCTCGCCAAGTGGGAATGCCTACTCCAGTCTTGCCAACAGGACTCCAGGGTTCGCT
NM_014862.3       ACCTCCTCGCCAAGTGGGAATGCCTACTCCAGTCTTGCCAACAGGACTCCAGGGTTCGCT
                  ************************************************************

HsCD00042627      GAAAGTGGACAAAGTAGCGGGCAGTTCCAAGGGCGGCCCTCGGAAGTCTGGTCGCAGTGG
NM_014862.3       GAAAGTGGACAAAGTAGCGGGCAGTTCCAAGGGCGGCCCTCGGAAGTCTGGTCGCAGTGG
                  ************************************************************

HsCD00042627      CAAAGCCAGCACCATGGCCAGCAGAGCGGTGAGCAGCACTCCCACCAGCAGCCCGGTCAG
NM_014862.3       CAAAGCCAGCACCATGGCCAGCAGAGCGGTGAGCAGCACTCCCACCAGCAGCCCGGTCAG
                  ************************************************************

HsCD00042627      ACTGAAGTGTTCCAGGACATGCTGCCCATGCCAGGAGATCCAGGGAATGAATGGTGGTCT
NM_014862.3       ACTGAAGTGTTCCAGGACATGCTGCCCATGCCAGGAGATCCAACCCAGGGGACTGGCAAC
                  ******************************************.  .* *..:   * :. 

HsCD00042627      CCCCACTCCCGGCAGCACTTTAGGCAGCCCATAAGCTATGCGAGAATGTAG---
NM_014862.3       TATAACATCGAAGACTTTGCCGACCTGGGCATGTTTCCACCGTTTTCTGAGTAG
                   . .**: * .. *  :    .. *:*  ***.:   .: **: ::   **   

Clone

Cloning Information : 168375
HIP Master Clone ID : 141841
Original Clone ID : FLH168375.01X

Organism


TAX_ID : 9606
Species Specific ID: 9915

Genome

Chromosome : 15
Map Location : 15q24
Ensembl : ENSG00000172379

Reagents

Labome : ARNT2-antibody