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TNFRSF21 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  TNFRSF21
Gene Name:  tumor necrosis factor receptor superfamily, member 21
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH165802.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00042786

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1968nts         Open reading frame : 1 to 1968
Reference Sequence Alignment

ATGGGGACCTCTCCGAGCAGCAGCACCGCCCTCGCCTCCTGCAGCCGCATCGCCCGCCGA
GCCACAGCCACGATGATCGCGGGCTCCCTTCTCCTGCTTGGATTCCTTAGCACCACCACA
GCTCAGCCAGAACAGAAGGCCTCGAATCTCATTGGCACATACCGCCATGTTGACCGTGCC
ACCGGCCAGGTGCTAACCTGTGACAAGTGTCCAGCAGGAACCTATGTCTCTGAGCATTGT
ACCAACACAAGCCTGCGCGTCTGCAGCAGTTGCCCTGTGGGGACCTTTACCAGGCATGAG
AATGGCATAGAGAAATGCCATGACTGTAGTCAGCCATGCCCATGGCCAATGATTGAGAAA
TTACCTTGTGCTGCCTTGACTGACCGAGAATGCACTTGCCCACCTGGCATGTTCCAGTCT
AACGCTACCTGTGCCCCCCATACGGTGTGTCCTGTGGGTTGGGGTGTGCGGAAGAAAGGG
ACAGAGACTGAGGATGTGCGGTGTAAGCAGTGTGCTCGGGGTACCTTCTCAGATGTGCCT
TCTAGTGTGATGAAATGCAAAGCATACACAGACTGTCTGAGTCAGAACCTGGTGGTGATC
AAGCCGGGGACCAAGGAGACAGACAACGTCTGTGGCACACTCCCGTCCTTCTCCAGCTCC
ACCTCACCTTCCCCTGGCACAGCCATCTTTCCACGCCCTGAGCACATGGAAACCCATGAA
GTCCCTTCCTCCACTTATGTTCCCAAAGGCATGAACTCAACAGAATCCAACTCTTCTGCC
TCTGTTAGACCAAAGGTACTGAGTAGCATCCAGGAAGGGACAGTCCCTGACAACACAAGC
TCAGCAAGGGGGAAGGAAGACGTGAACAAGACCCTCCCAAACCTTCAGGTAGTCAACCAC
CAGCAAGGCCCCCACCACAGACACATCCTGAAGCTGCTGCCGTCCATGGAGGCCACTGGG
GGCGAGAAGTCCAGCACGCCCATCAAGGGCCCCAAGAGGGGACATCCTAGACAGAACCTA
CACAAGCATTTTGACATCAATGAGCATTTGCCCTGGATGATTGTGCTTTTCCTGCTGCTG
GTGCTTGTGGTGATTGTGGTGTGCAGTATCCGGAAAAGCTCGAGGACTCTGAAAAAGGGG
CCCCGGCAGGATCCCAGTGCCATTGTGGAAAAGGCAGGGCTGAAGAAATCCATGACTCCA
ACCCAGAACCGGGAGAAATGGATCTACTACTGCAATGGCCATGGTATCGATATCCTGAAG
CTTGTAGCAGCCCAAGTGGGAAGCCAGTGGAAAGATATCTATCAGTTTCTTTGCAATGCC
AGTGAGAGGGAGGTTGCTGCTTTCTCCAATGGGTACACAGCCGACCACGAGCGGGCCTAC
GCAGCTCTGCAGCACTGGACCATCCGGGGCCCCGAGGCCAGCCTCGCCCAGCTAATTAGC
GCCCTGCGCCAGCACCGGAGAAACGATGTTGTGGAGAAGATTCGTGGGCTGATGGAAGAC
ACCACCCAGCTGGAAACTGACAAACTAGCTCTCCCGATGAGCCCCAGCCCGCTTAGCCCG
AGCCCCATCCCCAGCCCCAACGCGAAACTTGAGAATTCCGCTCTCCTGACGGTGGAGCCT
TCCCCACAGGACAAGAACAAGGGCTTCTTCGTGGATGAGTCGGAGCCCCTTCTCCGCTGT
GACTCTACATCCAGCGGCTCCTCCGCGCTGAGCAGGAACGGTTCCTTTATTACCAAAGAA
AAGAAGGACACAGTGTTGCGGCAGGTACGCCTGGACCCCTGTGACTTGCAGCCTATCTTT
GATGACATGCTCCACTTTCTAAATCCTGAGGAGCTGCGGGTGATTGAAGAGATTCCCCAG
GCTGAGGACAAACTAGACCGGCTATTCGAAATTATTGGAGTCAAGAGCCAGGAAGCCAGC
CAGACCCTCCTGGACTCTGTTTATAGCCATCTTCCTGACCTGCTGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1991
Start on reference sequence 24
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TNFRSF21
Symbol Nomenclature : TNFRSF21
SYNONYM : BM-018; CD358; DR6
Designation : TNFR-related death receptor 6|death receptor 6|tumor necrosis factor receptor superfamily member 21
Full Nomenclature : tumor necrosis factor receptor superfamily, member 21
GENEID : 27242
Locus Tag : UNQ437/PRO868
HGNC : 13469
MIM : 605732
Vega : OTTHUMG00000014796
Target GenBank: BC017730

 

Reference Sequence Alignment

  

HsCD00042786      ATGGGGACCTCTCCGAGCAGCAGCACCGCCCTCGCCTCCTGCAGCCGCATCGCCCGCCGA
NM_014452.4       ATGGGGACCTCTCCGAGCAGCAGCACCGCCCTCGCCTCCTGCAGCCGCATCGCCCGCCGA
                  ************************************************************

HsCD00042786      GCCACAGCCACGATGATCGCGGGCTCCCTTCTCCTGCTTGGATTCCTTAGCACCACCACA
NM_014452.4       GCCACAGCCACGATGATCGCGGGCTCCCTTCTCCTGCTTGGATTCCTTAGCACCACCACA
                  ************************************************************

HsCD00042786      GCTCAGCCAGAACAGAAGGCCTCGAATCTCATTGGCACATACCGCCATGTTGACCGTGCC
NM_014452.4       GCTCAGCCAGAACAGAAGGCCTCGAATCTCATTGGCACATACCGCCATGTTGACCGTGCC
                  ************************************************************

HsCD00042786      ACCGGCCAGGTGCTAACCTGTGACAAGTGTCCAGCAGGAACCTATGTCTCTGAGCATTGT
NM_014452.4       ACCGGCCAGGTGCTAACCTGTGACAAGTGTCCAGCAGGAACCTATGTCTCTGAGCATTGT
                  ************************************************************

HsCD00042786      ACCAACACAAGCCTGCGCGTCTGCAGCAGTTGCCCTGTGGGGACCTTTACCAGGCATGAG
NM_014452.4       ACCAACACAAGCCTGCGCGTCTGCAGCAGTTGCCCTGTGGGGACCTTTACCAGGCATGAG
                  ************************************************************

HsCD00042786      AATGGCATAGAGAAATGCCATGACTGTAGTCAGCCATGCCCATGGCCAATGATTGAGAAA
NM_014452.4       AATGGCATAGAGAAATGCCATGACTGTAGTCAGCCATGCCCATGGCCAATGATTGAGAAA
                  ************************************************************

HsCD00042786      TTACCTTGTGCTGCCTTGACTGACCGAGAATGCACTTGCCCACCTGGCATGTTCCAGTCT
NM_014452.4       TTACCTTGTGCTGCCTTGACTGACCGAGAATGCACTTGCCCACCTGGCATGTTCCAGTCT
                  ************************************************************

HsCD00042786      AACGCTACCTGTGCCCCCCATACGGTGTGTCCTGTGGGTTGGGGTGTGCGGAAGAAAGGG
NM_014452.4       AACGCTACCTGTGCCCCCCATACGGTGTGTCCTGTGGGTTGGGGTGTGCGGAAGAAAGGG
                  ************************************************************

HsCD00042786      ACAGAGACTGAGGATGTGCGGTGTAAGCAGTGTGCTCGGGGTACCTTCTCAGATGTGCCT
NM_014452.4       ACAGAGACTGAGGATGTGCGGTGTAAGCAGTGTGCTCGGGGTACCTTCTCAGATGTGCCT
                  ************************************************************

HsCD00042786      TCTAGTGTGATGAAATGCAAAGCATACACAGACTGTCTGAGTCAGAACCTGGTGGTGATC
NM_014452.4       TCTAGTGTGATGAAATGCAAAGCATACACAGACTGTCTGAGTCAGAACCTGGTGGTGATC
                  ************************************************************

HsCD00042786      AAGCCGGGGACCAAGGAGACAGACAACGTCTGTGGCACACTCCCGTCCTTCTCCAGCTCC
NM_014452.4       AAGCCGGGGACCAAGGAGACAGACAACGTCTGTGGCACACTCCCGTCCTTCTCCAGCTCC
                  ************************************************************

HsCD00042786      ACCTCACCTTCCCCTGGCACAGCCATCTTTCCACGCCCTGAGCACATGGAAACCCATGAA
NM_014452.4       ACCTCACCTTCCCCTGGCACAGCCATCTTTCCACGCCCTGAGCACATGGAAACCCATGAA
                  ************************************************************

HsCD00042786      GTCCCTTCCTCCACTTATGTTCCCAAAGGCATGAACTCAACAGAATCCAACTCTTCTGCC
NM_014452.4       GTCCCTTCCTCCACTTATGTTCCCAAAGGCATGAACTCAACAGAATCCAACTCTTCTGCC
                  ************************************************************

HsCD00042786      TCTGTTAGACCAAAGGTACTGAGTAGCATCCAGGAAGGGACAGTCCCTGACAACACAAGC
NM_014452.4       TCTGTTAGACCAAAGGTACTGAGTAGCATCCAGGAAGGGACAGTCCCTGACAACACAAGC
                  ************************************************************

HsCD00042786      TCAGCAAGGGGGAAGGAAGACGTGAACAAGACCCTCCCAAACCTTCAGGTAGTCAACCAC
NM_014452.4       TCAGCAAGGGGGAAGGAAGACGTGAACAAGACCCTCCCAAACCTTCAGGTAGTCAACCAC
                  ************************************************************

HsCD00042786      CAGCAAGGCCCCCACCACAGACACATCCTGAAGCTGCTGCCGTCCATGGAGGCCACTGGG
NM_014452.4       CAGCAAGGCCCCCACCACAGACACATCCTGAAGCTGCTGCCGTCCATGGAGGCCACTGGG
                  ************************************************************

HsCD00042786      GGCGAGAAGTCCAGCACGCCCATCAAGGGCCCCAAGAGGGGACATCCTAGACAGAACCTA
NM_014452.4       GGCGAGAAGTCCAGCACGCCCATCAAGGGCCCCAAGAGGGGACATCCTAGACAGAACCTA
                  ************************************************************

HsCD00042786      CACAAGCATTTTGACATCAATGAGCATTTGCCCTGGATGATTGTGCTTTTCCTGCTGCTG
NM_014452.4       CACAAGCATTTTGACATCAATGAGCATTTGCCCTGGATGATTGTGCTTTTCCTGCTGCTG
                  ************************************************************

HsCD00042786      GTGCTTGTGGTGATTGTGGTGTGCAGTATCCGGAAAAGCTCGAGGACTCTGAAAAAGGGG
NM_014452.4       GTGCTTGTGGTGATTGTGGTGTGCAGTATCCGGAAAAGCTCGAGGACTCTGAAAAAGGGG
                  ************************************************************

HsCD00042786      CCCCGGCAGGATCCCAGTGCCATTGTGGAAAAGGCAGGGCTGAAGAAATCCATGACTCCA
NM_014452.4       CCCCGGCAGGATCCCAGTGCCATTGTGGAAAAGGCAGGGCTGAAGAAATCCATGACTCCA
                  ************************************************************

HsCD00042786      ACCCAGAACCGGGAGAAATGGATCTACTACTGCAATGGCCATGGTATCGATATCCTGAAG
NM_014452.4       ACCCAGAACCGGGAGAAATGGATCTACTACTGCAATGGCCATGGTATCGATATCCTGAAG
                  ************************************************************

HsCD00042786      CTTGTAGCAGCCCAAGTGGGAAGCCAGTGGAAAGATATCTATCAGTTTCTTTGCAATGCC
NM_014452.4       CTTGTAGCAGCCCAAGTGGGAAGCCAGTGGAAAGATATCTATCAGTTTCTTTGCAATGCC
                  ************************************************************

HsCD00042786      AGTGAGAGGGAGGTTGCTGCTTTCTCCAATGGGTACACAGCCGACCACGAGCGGGCCTAC
NM_014452.4       AGTGAGAGGGAGGTTGCTGCTTTCTCCAATGGGTACACAGCCGACCACGAGCGGGCCTAC
                  ************************************************************

HsCD00042786      GCAGCTCTGCAGCACTGGACCATCCGGGGCCCCGAGGCCAGCCTCGCCCAGCTAATTAGC
NM_014452.4       GCAGCTCTGCAGCACTGGACCATCCGGGGCCCCGAGGCCAGCCTCGCCCAGCTAATTAGC
                  ************************************************************

HsCD00042786      GCCCTGCGCCAGCACCGGAGAAACGATGTTGTGGAGAAGATTCGTGGGCTGATGGAAGAC
NM_014452.4       GCCCTGCGCCAGCACCGGAGAAACGATGTTGTGGAGAAGATTCGTGGGCTGATGGAAGAC
                  ************************************************************

HsCD00042786      ACCACCCAGCTGGAAACTGACAAACTAGCTCTCCCGATGAGCCCCAGCCCGCTTAGCCCG
NM_014452.4       ACCACCCAGCTGGAAACTGACAAACTAGCTCTCCCGATGAGCCCCAGCCCGCTTAGCCCG
                  ************************************************************

HsCD00042786      AGCCCCATCCCCAGCCCCAACGCGAAACTTGAGAATTCCGCTCTCCTGACGGTGGAGCCT
NM_014452.4       AGCCCCATCCCCAGCCCCAACGCGAAACTTGAGAATTCCGCTCTCCTGACGGTGGAGCCT
                  ************************************************************

HsCD00042786      TCCCCACAGGACAAGAACAAGGGCTTCTTCGTGGATGAGTCGGAGCCCCTTCTCCGCTGT
NM_014452.4       TCCCCACAGGACAAGAACAAGGGCTTCTTCGTGGATGAGTCGGAGCCCCTTCTCCGCTGT
                  ************************************************************

HsCD00042786      GACTCTACATCCAGCGGCTCCTCCGCGCTGAGCAGGAACGGTTCCTTTATTACCAAAGAA
NM_014452.4       GACTCTACATCCAGCGGCTCCTCCGCGCTGAGCAGGAACGGTTCCTTTATTACCAAAGAA
                  ************************************************************

HsCD00042786      AAGAAGGACACAGTGTTGCGGCAGGTACGCCTGGACCCCTGTGACTTGCAGCCTATCTTT
NM_014452.4       AAGAAGGACACAGTGTTGCGGCAGGTACGCCTGGACCCCTGTGACTTGCAGCCTATCTTT
                  ************************************************************

HsCD00042786      GATGACATGCTCCACTTTCTAAATCCTGAGGAGCTGCGGGTGATTGAAGAGATTCCCCAG
NM_014452.4       GATGACATGCTCCACTTTCTAAATCCTGAGGAGCTGCGGGTGATTGAAGAGATTCCCCAG
                  ************************************************************

HsCD00042786      GCTGAGGACAAACTAGACCGGCTATTCGAAATTATTGGAGTCAAGAGCCAGGAAGCCAGC
NM_014452.4       GCTGAGGACAAACTAGACCGGCTATTCGAAATTATTGGAGTCAAGAGCCAGGAAGCCAGC
                  ************************************************************

HsCD00042786      CAGACCCTCCTGGACTCTGTTTATAGCCATCTTCCTGACCTGCTGTAG
NM_014452.4       CAGACCCTCCTGGACTCTGTTTATAGCCATCTTCCTGACCTGCTGTAG
                  ************************************************

Pathways

Reactome : Attenuation phase
Reactome : Cellular response to heat stress
Reactome : Cellular responses to stress
Reactome : Fatty acid, triacylglycerol, and ketone body metabolism
Reactome : HSF1 activation
Reactome : HSF1-dependent transactivation
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : PPARA activates gene expression
Reactome : Regulation of HSF1-mediated heat shock response
Reactome : Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)
WikiPathway : Apoptosis

Clone

Cloning Information : 165802
HIP Master Clone ID : 138966
Original Clone ID : FLH165802.01X

Organism


TAX_ID : 9606
Species Specific ID: 27242

Genome

Chromosome : 6
Map Location : 6p21.1
Ensembl : ENSG00000146072

Reagents

Labome : DR6-antibody