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CA8 (Homo sapiens) in pVP16 (His and MBP-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CA8
Gene Name:  carbonic anhydrase VIII
Sequence             
Original Clone ID: 5901.1.80_GO.57446
Keyword: full-length cds
Species: Homo sapiens
Type: cDNA
Vector Name: pVP16               Format:  CLOSED
Source: Center for Eukaryotic Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
HsCD00429580

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 891nts         Open reading frame : 51 to 941

GGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCGCGGACCTGAGCTTCATCGAAGATACCGTCGCCTTCCCCGAGAAGGAAG
AGGATGAGGAGGAAGAAGAGGAGGGTGTGGAGTGGGGCTACGAGGAAGGTGTTGAGTGGG
GTCTGGTGTTTCCTGATGCTAATGGGGAATACCAGTCTCCTATTAACCTAAACTCAAGAG
AGGCTAGGTATGACCCCTCGCTGTTGGATGTCCGCCTCTCCCCAAATTATGTGGTGTGCC
GAGACTGTGAAGTCACCAATGATGGACATACCATTCAGGTTATCCTGAAGTCAAAATCAG
TTCTTTCGGGAGGACCATTGCCTCAAGGGCATGAATTTGAACTGTACGAAGTGAGATTTC
ACTGGGGAAGAGAAAACCAGCGTGGTTCTGAGCACACGGTTAATTTCAAAGCTTTTCCCA
TGGAGCTCCATCTGATCCACTGGAACTCCACTCTGTTTGGCAGCATTGATGAGGCTGTGG
GGAAGCCGCACGGAATCGCCATCATTGCTCTGTTTGTTCAGATAGGAAAGGAACATGTTG
GCTTGAAGGCTGTGACTGAAATCCTCCAAGATATTCAGTATAAGGGGAAGTCCAAAACAA
TACCTTGCTTTAATCCTAACACTTTATTACCAGACCCTCTGCTGCGGGATTACTGGGTGT
ATGAAGGCTCTCTCACCATCCCACCTTGCAGTGAAGGTGTCACCTGGATATTATTCCGAT
ACCCTTTAACTATATCCCAGCTACAGATAGAAGAATTTCGAAGGCTGAGGACACATGTTA
AGGGGGCAGAACTTGTGGAAGGCTGTGATGGGATTTTGGGAGACAACTTTCGGCCCACTC
AGCCTCTTAGTGACAGAGTCATTAGAGCTGCATTTCAGTAGTAGGACCCAGCTTTCTTGT
ACAAAGTGGTGATGGCGCGCCTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
ins@886,3
                                

5' Linker Sequence:
ACAAGTTTGTACAAAAAAGCAGGCTCC

3' Linker Sequence:
GACCCAGCTTTCTTGTACAAAGTGGT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Tested_Not_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in GO.57446
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pVP16

pVP16 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  MBP Forward
Reverse:  pQE Reverse
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
Comments: TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette
Size (bp): 7631
Parent Vector: None
Empty Vector: EvNO00084279
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 15750721
Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 6471 5789
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 2643 2852
promoter T5 T5 promoter 5 54
recombination site AttR1 AttR1 recombination site 1316 1440
recombination site AttR2 AttR2 recombination site 3020 2896
repressor protein gene LacI LacI coding region 4132 5211
selectable marker AmpR ampicillin resistance gene 7228 6569
selectable marker CmR chloramphenicol resistance gene (CmR) (Lost in with-insert form) 1549 2205
tag His N-terminal 8xHis tag 128 145
tag MBP N-terminal MBP 148 1246

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CA8
Symbol Nomenclature : CA8
SYNONYM : CA-VIII; CALS; CAMRQ3; CARP
Designation : CA-related protein|carbonate dehydratase|carbonic anhydrase-like sequence|carbonic anhydrase-related protein
Full Nomenclature : carbonic anhydrase VIII
GENEID : 767
GI : 22027500
GenBank Accession : NM_004056
HGNC : 1382
MIM : 114815
Target GenBank: NM_004056

 

Protein

SMART domain : Carb_anhydrase : Eukaryotic-type carbonic anhydrase
HPRD : 00266

Clone

Cloning Information : 510707
HIP Master Clone ID : 421874
Original Clone ID : 5901.1.80_GO.57446

Experimental

TargetTrack - Experimental data : GO.57446

Organism


TAX_ID : 9606
Species Specific ID: 767

Genome

Chromosome : 8
Map Location : 8q12.1

Reagents

Labome : CA8-antibody