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Atu3934 (A. tumefaciens ) in pSGX4 (His and Smt3-tagged bacterial expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  Atu3934
Gene Name:  None
Sequence             
Original Clone ID: 9252b2BSt5p1
Keyword: full-length cds
Species: Agrobacterium tumefaciens
Type: cDNA
Vector Name: pSGX4               Format:  CLOSED
Source: New York SGX Research Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 17640072
Title: X-ray structure of imidazolonepropionase from Agrobacterium tumefaciens at 1.87 A resolution
Authors: PSI
New York SGX Research Center for Structural Genomics
AtCD00430336

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1188nts         Open reading frame : 51 to 1238

AGGGAACGGCGACGGGGAACGCCACGGCTTTGTGGCGCAACGCCCAGCTGGCGACCCTCA
ATCCCGCCATGGATGGCATCGGCGCCGTCGAAAACGCCGTTATTGCCGTGCGTAACGGCC
GCATTGCCTTTGCCGGCCCGGAAAGCGATTTGCCCGATGACCTCTCGACAGCCGATGAGA
CGACCGATTGCGGCGGCCGCTGGATCACACCCGCCTTGATTGACTGCCACACCCATCTCG
TCTTTGGCGGCAATCGCGCCATGGAATTCGAAATGCGGCTCAATGGCGCCACCTATGAAG
AGATCGCCAAAGCGGGCGGCGGCATCGTTTCTTCGGTGCGCGACACGCGCGCGCTTTCGG
ACGAGGTTTTGGTGGCGCAGGCTCTGCCGCGTCTCGACACGCTTCTTTCCGAGGGTGTTT
CCACCATCGAAATCAAATCCGGTTATGGTCTCGACATCGAAACCGAGCTGAAAATGCTGC
GCGTCGCCCGCAGACTCGAGACATTACGGCCCGTCCGCATCGTCACCAGCTATCTCGCCG
CACACGCGACACCCGCGGACTATAAGGGCCGCAACGCCGATTACATCACCGACGTCGTTC
TCCCCGGGCTGGAAAAAGCCCATGCGGAAGGATTGGCGGATGCGGTGGACGGTTTTTGCG
AAGGCATCGCCTTTTCCGTGAAAGAGATCGACCGGGTTTTTGCGGCAGCACAGCAGCGGG
GGCTCCCCGTCAAACTTCATGCCGAACAGCTTTCCAATCTCGGCGGCGCAGAGCTTGCGG
CCTCCTACAACGCGCTTTCGGCCGATCATCTGGAATATCTCGACGAGACCGGTGCGAAGG
CGCTGGCAAAAGCCGGAACCGTGGCCGTGCTTCTGCCCGGCGCCTTTTATGCGCTCAGGG
AGAAGCAACTGCCGCCGGTTCAGGCACTGCGCGATGCCGGAGCCGAGATCGCGCTCGCAA
CGGATTGCAACCCCGGCACCTCGCCGCTCACCTCGCTGCTGCTGACCATGAATATGGGCG
CCACGCTTTTCCGCATGACAGTGGAGGAGTGCCTGACGGCAACGACACGCAATGCGGCAA
AGGCGCTCGGACTGCTTGCGGAAACCGGCACGCTGGAGGCCGGAAAATCCGCCGATTTCG
CCATCTGGGACATCGAGCGCCCGGCCGAGCTTGTCTATCGCATCGGCTTCAACCCGCTCC
ATGCCCGTATTTTCAAGGGACAGAAGGTTTCTCCATAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ACAGAGAACAGATTGGTGGATCCCTT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-9252b
Recommended expression in: BL21(DE3)-Codon+RIL
       DyNA Vector Map

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Vector Name: pSGX4

pSGX4 in advanced viewed

Synonyms: pSGX-4, pSGX4_BS
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promotor, adds N-terminal 6xHis and smt-3 tags, cleavage site; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen. N-terminal 6xHis-tag followed by smt3 fusion protein. Smt3 fusion cloned into NheI and BamHI sites. TOPO adaptation at BamHI and XhoI sites.
Size (bp): 5611
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
TOPO recognition site TOPO TOPO cloning site 5435 5444
bacterial origin ColE ColE1 bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4984 5003
protease cleavage site ULP site ULP cleavage site (recognizes the tertiary structure of SUMO) 5140 5140
repressor protein gene LacI lacI cds 3515 4351
ribosome binding site RBS ribosome binding site (RBS) 5062 5068
selectable marker KanR kanamycin resistance gene 563 1375
tag Smt3 Smt3 tag 5140 5424
tag His 6xHis tag 5083 5100
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 termination sequence 5539 5585

 

  • Gene
  • Protein
  • Clone
  • Experimental

Gene

Target GenBank: None

 

Protein

PDB - Structural Annotation : 2GOK
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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Clone

Cloning Information : 494413
HIP Master Clone ID : 408093
Original Clone ID : 9252b2BSt5p1

Experimental

Structural Biology Knowledgebase : SBKB (2GOK)
TargetTrack - Experimental data : NYSGXRC-9252b