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EIF3S2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  EIF3I
Gene Name:  eukaryotic translation initiation factor 3, subunit I
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH176816.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00043266

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 978nts         Open reading frame : 1 to 978
Reference Sequence Alignment

ATGAAGCCGATCCTACTGCAGGGCCATGAGCGGTCCATTACGCAGATTAAGTATAACCGC
GAAGGAGACCTCCTCTTTACTGTGGCCAAGGACCCTATCGTCAATGTATGGTACTCTGTG
AATGGTGAGAGGCTGGGCACCTACATGGGCCATACCGGAGCTGTGTGGTGTGTGGACGCT
GACTGGGACACCAAGCATGTCCTCACTGGCTCAGCTGACAACAGCTGTCGTCTCTGGGAC
TGTGAAACAGGAAAGCAGCTGGCCCTTCTCAAGACCAATTCGGCTGTCCGGACCTGCGGT
TTTGACTTTGGGGGCAACATCATCATGTTCTCCACGGACAAGCAGATGGGCTACCAGTGC
TTTGTGAGCTTTTTTGACCTGCGGGATCCGAGCCAGATTGACAACAATGAGCCCTACATG
AAGATCCCTTGCAATGACTCTAAAATCACCAGTGCTGTTTGGGGACCCCTGGGGGAGTGC
ATCATCGCTGGCCATGAGAGTGGAGAGCTCAACCAGTATAGTGCCAAGTCTGGAGAGGTG
TTGGTGAATGTTAAGGAGCACTCCCGGCAGATCAACGACATCCAGTTATCCAGGGACATG
ACCATGTTTGTGACCGCGTCCAAGGACAACACAGCCAAGCTTTTTGACTCCACAACTCTT
GAACATCAGAAGACTTTCCGGACAGAACGTCCTGTCAACTCAGCTGCCCTCTCCCCCAAC
TATGACCATGTGGTCCTGGGCGGTGGTCAGGAAGCCATGGATGTAACCACAACCTCCACC
AGGATTGGCAAGTTTGAGGCCAGGTTCTTCCATTTGGCCTTTGAAGAAGAGTTTGGAAGA
GTCAAGGGTCACTTTGGACCTATCAACAGTGTTGCCTTCCATCCTGATGGCAAGAGCTAC
AGCAGCGGCGGCGAAGATGGTTACGTCCGTATCCATTACTTCGACCCACAGTACTTCGAA
TTTGAGTTTGAGGCTTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1001
Start on reference sequence 24
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : EIF3I
Symbol Nomenclature : EIF3I
SYNONYM : EIF3S2; PRO2242; TRIP-1; TRIP1; eIF3-beta; eIF3-p36
Designation : TGF-beta receptor-interacting protein 1|TGFbeta receptor-interacting protein 1|eIF-3-beta|eIF3 p36|eukaryotic translation initiation factor 3 subunit 2|eukaryotic translation initiation factor 3 subunit I|eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD)|eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa|predicted protein of HQ2242
Full Nomenclature : eukaryotic translation initiation factor 3, subunit I
GENEID : 8668
Locus Tag : RP4-675E8.1
HGNC : 3272
MIM : 603911
Vega : OTTHUMG00000007364
Target GenBank: BC000413

 

Reference Sequence Alignment

  

HsCD00043266      ATGAAGCCGATCCTACTGCAGGGCCATGAGCGGTCCATTACGCAGATTAAGTATAACCGC
NM_003757.2       ATGAAGCCGATCCTACTGCAGGGCCATGAGCGGTCCATTACGCAGATTAAGTATAACCGC
                  ************************************************************

HsCD00043266      GAAGGAGACCTCCTCTTTACTGTGGCCAAGGACCCTATCGTCAATGTATGGTACTCTGTG
NM_003757.2       GAAGGAGACCTCCTCTTTACTGTGGCCAAGGACCCTATCGTCAATGTATGGTACTCTGTG
                  ************************************************************

HsCD00043266      AATGGTGAGAGGCTGGGCACCTACATGGGCCATACCGGAGCTGTGTGGTGTGTGGACGCT
NM_003757.2       AATGGTGAGAGGCTGGGCACCTACATGGGCCATACCGGAGCTGTGTGGTGTGTGGACGCT
                  ************************************************************

HsCD00043266      GACTGGGACACCAAGCATGTCCTCACTGGCTCAGCTGACAACAGCTGTCGTCTCTGGGAC
NM_003757.2       GACTGGGACACCAAGCATGTCCTCACTGGCTCAGCTGACAACAGCTGTCGTCTCTGGGAC
                  ************************************************************

HsCD00043266      TGTGAAACAGGAAAGCAGCTGGCCCTTCTCAAGACCAATTCGGCTGTCCGGACCTGCGGT
NM_003757.2       TGTGAAACAGGAAAGCAGCTGGCCCTTCTCAAGACCAATTCGGCTGTCCGGACCTGCGGT
                  ************************************************************

HsCD00043266      TTTGACTTTGGGGGCAACATCATCATGTTCTCCACGGACAAGCAGATGGGCTACCAGTGC
NM_003757.2       TTTGACTTTGGGGGCAACATCATCATGTTCTCCACGGACAAGCAGATGGGCTACCAGTGC
                  ************************************************************

HsCD00043266      TTTGTGAGCTTTTTTGACCTGCGGGATCCGAGCCAGATTGACAACAATGAGCCCTACATG
NM_003757.2       TTTGTGAGCTTTTTTGACCTGCGGGATCCGAGCCAGATTGACAACAATGAGCCCTACATG
                  ************************************************************

HsCD00043266      AAGATCCCTTGCAATGACTCTAAAATCACCAGTGCTGTTTGGGGACCCCTGGGGGAGTGC
NM_003757.2       AAGATCCCTTGCAATGACTCTAAAATCACCAGTGCTGTTTGGGGACCCCTGGGGGAGTGC
                  ************************************************************

HsCD00043266      ATCATCGCTGGCCATGAGAGTGGAGAGCTCAACCAGTATAGTGCCAAGTCTGGAGAGGTG
NM_003757.2       ATCATCGCTGGCCATGAGAGTGGAGAGCTCAACCAGTATAGTGCCAAGTCTGGAGAGGTG
                  ************************************************************

HsCD00043266      TTGGTGAATGTTAAGGAGCACTCCCGGCAGATCAACGACATCCAGTTATCCAGGGACATG
NM_003757.2       TTGGTGAATGTTAAGGAGCACTCCCGGCAGATCAACGACATCCAGTTATCCAGGGACATG
                  ************************************************************

HsCD00043266      ACCATGTTTGTGACCGCGTCCAAGGACAACACAGCCAAGCTTTTTGACTCCACAACTCTT
NM_003757.2       ACCATGTTTGTGACCGCGTCCAAGGACAACACAGCCAAGCTTTTTGACTCCACAACTCTT
                  ************************************************************

HsCD00043266      GAACATCAGAAGACTTTCCGGACAGAACGTCCTGTCAACTCAGCTGCCCTCTCCCCCAAC
NM_003757.2       GAACATCAGAAGACTTTCCGGACAGAACGTCCTGTCAACTCAGCTGCCCTCTCCCCCAAC
                  ************************************************************

HsCD00043266      TATGACCATGTGGTCCTGGGCGGTGGTCAGGAAGCCATGGATGTAACCACAACCTCCACC
NM_003757.2       TATGACCATGTGGTCCTGGGCGGTGGTCAGGAAGCCATGGATGTAACCACAACCTCCACC
                  ************************************************************

HsCD00043266      AGGATTGGCAAGTTTGAGGCCAGGTTCTTCCATTTGGCCTTTGAAGAAGAGTTTGGAAGA
NM_003757.2       AGGATTGGCAAGTTTGAGGCCAGGTTCTTCCATTTGGCCTTTGAAGAAGAGTTTGGAAGA
                  ************************************************************

HsCD00043266      GTCAAGGGTCACTTTGGACCTATCAACAGTGTTGCCTTCCATCCTGATGGCAAGAGCTAC
NM_003757.2       GTCAAGGGTCACTTTGGACCTATCAACAGTGTTGCCTTCCATCCTGATGGCAAGAGCTAC
                  ************************************************************

HsCD00043266      AGCAGCGGCGGCGAAGATGGTTACGTCCGTATCCATTACTTCGACCCACAGTACTTCGAA
NM_003757.2       AGCAGCGGCGGCGAAGATGGTTACGTCCGTATCCATTACTTCGACCCACAGTACTTCGAA
                  ************************************************************

HsCD00043266      TTTGAGTTTGAGGCTTAG
NM_003757.2       TTTGAGTTTGAGGCTTAA
                  *****************.

Pathways

Reactome : 3' -UTR-mediated translational regulation
Reactome : Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S
Reactome : Cap-dependent Translation Initiation
Reactome : Eukaryotic Translation Initiation
Reactome : Formation of a pool of free 40S subunits
Reactome : Formation of the ternary complex, and subsequently, the 43S complex
Reactome : GTP hydrolysis and joining of the 60S ribosomal subunit
Reactome : Gene Expression
Reactome : L13a-mediated translational silencing of Ceruloplasmin expression
Reactome : Metabolism of proteins
Reactome : Ribosomal scanning and start codon recognition
Reactome : Translation
Reactome : Translation initiation complex formation
WikiPathway : Translation Factors

Protein

SMART domain : WD40 : WD40 repeats
UniProt : Q13347
UniProt : Q5U0F4
HPRD : 04884

Clone

Cloning Information : 176816
HIP Master Clone ID : 151961
Original Clone ID : FLH176816.01X

Organism


TAX_ID : 9606
Species Specific ID: 8668

Genome

Chromosome : 1
Map Location : 1p34.1
Ensembl : ENSG00000084623

Reagents

Labome : EIF3I-antibody