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agaR (K. pneumoniae subsp. pneumoniae MGH 78578 ) in pMCSG19

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Explanation of Terms


Gene: Gene Symbol:  agaR
Gene Name:  regulator of aga operon
Sequence             
Original Clone ID: APC36500_SG205_pMCSG19
Keyword: full length
Species: Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Type: cDNA
Vector Name: pMCSG19               Format:  CLOSED
Source: Midwest Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Midwest Center for Structural Genomics
Shonda Clancy
KpCD00432749

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 774nts         Open reading frame : 45 to 818

TTCCTCGGGGGATCGGGTACGAAACCTGTACTTCCATCCATGCCATGAACTCATTTGAAC
GAAGGAACAAAATTGTCGATATGGTGAATACGCAGGGTAGCGTATTGGTTCTAGACCTTT
CGAATCTCTTTGGCATCTCTGAGGTAACGATCCGTGCCGATCTCCGCTTACTGGAAGAAA
AAGGACTGTTAACCCGTTTTCACGGCGGCGCCGCCCGTGCGGGTAGCGACCTGACCGACG
CCGAGAATCAGGAAGTGGTGCTTGAAGACCGCTATCAGCTTGCCAGCGATCCCAAAAAGC
GCATCGCCCATGCCGCGGCAGCGATGATCCGTGAAGGTATGACGGTAATCCTCGATAGCG
GTAGCACCACTCTTCTTATCGCCGAGGCATTAGTACGGATGACCAATATCACCGTCATCA
CCAACAGCCTGCCCGCCGCGTTTACTCTGGCAGAAAATAAAGATATCACCCTGGTGGTGT
GCGGCGGTACCGTGCGGCACAAAACGCATTCGATGCACGGCACGATTGCCGAACGTTCGC
TGCAAGGTATTTGCGCCGATTTGATGTTTGTCGGCGCCGACGGTATCGACGCCACCAATG
GCATCACCACCTTTAACGAAGGCTACTCCATCAGCAGCGTGATGGCCGCCGCCGCGCATC
AGGTGATCGCCGTGCTCGACGCCAGCAAATTCAATCGCCGGGGCTTTAACCAGGTACTGC
CGATGGAAAAGATAGACTGCGTGATCACCGACGACGGCATTAGTGAAAATGACCGTGCAG
CGTTAAGCAGCAAAAAGGTGGAACTGAAGATAGTGTAACATTGGAAGTGGATAACGGATC
CGAATTCGAGCTCCGTCGACAAGCTTGCG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GCC

3' Linker Sequence:
TAAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Not_Applicable
SolubleProtein: Protein_Soluble
Find experimental protocols in APC36500
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pMCSG19

pMCSG19 in advanced viewed

Synonyms: pMCSG-19
Sequencing Primer: Forward:  ANForward
Reverse:  T7 terminator
Description: Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC).
Comments: pMCSG7 derivative: in vivo TVMV cleavage of MBP
Size (bp): 6441
Parent Vector: None
Empty Vector: EvNO00084265
Properties: bacterial expression, improves solubility of insert, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 16497515
Title: An expression vector tailored for large-scale, high-throughput purification of recombinant proteins

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC Ligation independent cloning (LIC) site 204 235
bacterial origin ori ColE1-type bacterial origin of replication 4416 5098
promoter T7 T7 promoter 1515 1531
protease cleavage site TVMV TVMV protease cleavage site 286 306
protease cleavage site TEV TEV cleavage site cds 223 243
repressor binding site lac operator LacO fragment 1490 1512
repressor protein gene LacI LacI coding sequence 2045 3004
selectable marker AmpR ampicillin resistance gene 5193 6053
tag His 2 C-terminal 6xHis tag 140 157
tag His 6xHis tag 268 285
tag MBP N-terminal MBP 337 1443
trxn termination sequence T7 term T7 terminator sequence 26 72

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism

Gene

 

Gene Symbol : agaR
Designation : putative DEOR-type transcriptional regulator
GENEID : 5339116
Locus Tag : KPN_03552
GI : 150956917
Target GenBank: None

 

Clone

Cloning Information : 526177
HIP Master Clone ID : 436497
Original Clone ID : APC36500_SG205_pMCSG19

Experimental

TargetTrack - Experimental data : APC36500

Organism


TAX_ID : 272620
Species Specific ID: 5339116