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ACTRT2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTRT2
Gene Name:  actin-related protein T2
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH170511.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00043277

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1131nts        
Open reading frame : 1 to 1131
Reference Sequence Alignment

ATGTTTAATCCGCATGCTTTAGACTCCCCGGCTGTGATTTTTGACAATGGCTCGGGGTTC
TGCAAAGCGGGCCTGTCTGGGGAGTTTGGACCCCGGCACATGGTCAGCTCCATCGTGGGG
CACCTGAAATTCCAGGCTCCCTCAGCAGAGGCCAACCAGAAGTACTTTGTGGGGGAGGAG
GCCCTGTACAAGCAGGAGGCCCTGCAGCTGCACTCCCCTTTCGAGCGTGGCCTGATCACA
GGGTGGGATGACGTGGAGAGACTCTGGAAGCACCTCTTTGAGTGGGAGCTAGGCGTGAAA
CCCAGCGACCAGCCCCTGCTTGCAACGGAGCCCTCCCTGAACCCCAGGGAGAACCGTGAG
AAGATGGCAGAAGTCATGTTCGAGAACTTCGGCGTGCCCGCTTTCTACCTGTCGGACCAG
GCGGTGCTGGCTCTCTACGCCTCGGCCTGTGTCACGGGCCTGGTGGTGGACAGCGGGGAT
GCGGTCACCTGCACTGTCCCCATCTTTGAGGGTTACTCCCTGCCCCACGCAGTCACCAAG
CTCCACGTGGCGGGCAGGGACATCACGGAGCTCCTCATGCAGCTGCTCCTGGCCAGCGGC
CACACCTTCCCCTGCCAGCTGGACAAGGGTCTCGTGGACGACATCAAAAAGAAGCTGTGC
TACGTGGCCTTGGAGCCCGAGAAGGAGCTTTCCCGGAGGCCGGAGGAGGTCCTGAGGGAG
TACAAGCTGCCCGACGGGAACATCATCAGCCTCGGGGACCCGCTGCACCAGGCGCCCGAG
GCCCTGTTCGTGCCCCAGCAGCTGGGCAGCCAGAGCCCCGGGCTCTCGAATATGGTCTCC
AGCAGCATCACCAAGTGTGATACCGACATCCAGAAGATCCTCTTTGGGGAGATTGTGCTG
TCGGGGGGCACTACCCTGTTCCACGGGCTGGATGACCGGCTTCTCAAGGAGCTGGAGCAG
CTGGCCTCCAAGGACACCCCCATCAAGATCACGGCTCCCCCCGACCGGTGGTTCTCCACC
TGGATTGGAGCCTCCATCGTCACCTCTCTGAGTAGCTTCAAGCAGATGTGGGTCACCGCC
GCAGACTTCAAGGAGTTTGGGACCTCCGTGGTGCAGAGAAGATGCTTCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1317
Start on reference sequence 187
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site U19 primer 517 535
primer site Att P2 Primer 3026 3043
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTRT2
Symbol Nomenclature : ACTRT2
SYNONYM : ARPM2; ARPT2; Arp-T2; HARPM2
Designation : actin-related protein M2|actin-related protein hArpM2
Full Nomenclature : actin-related protein T2
GENEID : 140625
HGNC : 24026
MIM : 608535
Vega : OTTHUMG00000000562
Target GenBank: BC029499

 

Reference Sequence Alignment

  

HsCD00043277      ATGTTTAATCCGCATGCTTTAGACTCCCCGGCTGTGATTTTTGACAATGGCTCGGGGTTC
NM_080431.4       ATGTTTAATCCGCACGCTTTAGACTCCCCGGCTGTGATTTTTGACAATGGCTCGGGGTTC
                  ************** *********************************************

HsCD00043277      TGCAAAGCGGGCCTGTCTGGGGAGTTTGGACCCCGGCACATGGTCAGCTCCATCGTGGGG
NM_080431.4       TGCAAAGCGGGCCTGTCTGGGGAGTTTGGACCCCGGCACATGGTCAGCTCCATCGTGGGG
                  ************************************************************

HsCD00043277      CACCTGAAATTCCAGGCTCCCTCAGCAGAG---GCCAACCAGAAGTACTTTGTGGGGGAG
NM_080431.4       CACCTGAAATTCCAGGCTCCCTCAGCAGAGGCCAACCAGAAGAAGTACTTTGTGGGGGAG
                  ******************************   ..*.* .********************

HsCD00043277      GAGGCCCTGTACAAGCAGGAGGCCCTGCAGCTGCACTCCCCTTTCGAGCGTGGCCTGATC
NM_080431.4       GAGGCCCTGTACAAGCAGGAGGCCCTGCAGCTGCACTCCCCTTTCGAGCGTGGCCTGATC
                  ************************************************************

HsCD00043277      ACAGGGTGGGATGACGTGGAGAGACTCTGGAAGCACCTCTTTGAGTGGGAGCTAGGCGTG
NM_080431.4       ACAGGGTGGGATGACGTGGAGAGACTCTGGAAGCACCTCTTTGAGTGGGAGCTAGGCGTG
                  ************************************************************

HsCD00043277      AAACCCAGCGACCAGCCCCTGCTTGCAACGGAGCCCTCCCTGAACCCCAGGGAGAACCGT
NM_080431.4       AAACCCAGCGACCAGCCCCTGCTTGCAACGGAGCCCTCCCTGAACCCCAGGGAGAACCGT
                  ************************************************************

HsCD00043277      GAGAAGATGGCAGAAGTCATGTTCGAGAACTTCGGCGTGCCCGCTTTCTACCTGTCGGAC
NM_080431.4       GAGAAGATGGCAGAAGTCATGTTCGAGAACTTCGGCGTGCCCGCTTTCTACCTGTCGGAC
                  ************************************************************

HsCD00043277      CAGGCGGTGCTGGCTCTCTACGCCTCGGCCTGTGTCACGGGCCTGGTGGTGGACAGCGGG
NM_080431.4       CAGGCGGTGCTGGCTCTCTACGCCTCTGCCTGTGTCACGGGCCTGGTGGTGGACAGCGGG
                  ************************** *********************************

HsCD00043277      GATGCGGTCACCTGCACTGTCCCCATCTTTGAGGGTTACTCCCTGCCCCACGCAGTCACC
NM_080431.4       GATGCGGTCACCTGCACTGTCCCCATCTTTGAGGGTTACTCCCTGCCCCACGCAGTCACC
                  ************************************************************

HsCD00043277      AAGCTCCACGTGGCGGGCAGGGACATCACGGAGCTCCTCATGCAGCTGCTCCTGGCCAGC
NM_080431.4       AAGCTCCACGTGGCGGGCAGGGACATCACGGAGCTCCTCATGCAGCTGCTCCTGGCCAGC
                  ************************************************************

HsCD00043277      GGCCACACCTTCCCCTGCCAGCTGGACAAGGGTCTCGTGGACGACATCAAAAAGAAGCTG
NM_080431.4       GGCCACACCTTCCCCTGCCAGCTGGACAAGGGTCTCGTGGACGACATCAAAAAGAAGCTG
                  ************************************************************

HsCD00043277      TGCTACGTGGCCTTGGAGCCCGAGAAGGAGCTTTCCCGGAGGCCGGAGGAGGTCCTGAGG
NM_080431.4       TGCTACGTGGCCTTGGAGCCCGAGAAGGAGCTTTCCCGGAGGCCGGAGGAGGTCCTGAGG
                  ************************************************************

HsCD00043277      GAGTACAAGCTGCCCGACGGGAACATCATCAGCCTCGGGGACCCGCTGCACCAGGCGCCC
NM_080431.4       GAGTACAAGCTGCCCGACGGGAACATCATCAGCCTCGGGGACCCGCTGCACCAGGCGCCC
                  ************************************************************

HsCD00043277      GAGGCCCTGTTCGTGCCCCAGCAGCTGGGCAGCCAGAGCCCCGGGCTCTCGAATATGGTC
NM_080431.4       GAGGCCCTGTTCGTGCCCCAGCAGCTGGGCAGCCAGAGCCCCGGGCTCTCGAATATGGTC
                  ************************************************************

HsCD00043277      TCCAGCAGCATCACCAAGTGTGATACCGACATCCAGAAGATCCTCTTTGGGGAGATTGTG
NM_080431.4       TCCAGCAGCATCACCAAGTGTGATACCGACATCCAGAAGATCCTCTTTGGGGAGATTGTG
                  ************************************************************

HsCD00043277      CTGTCGGGGGGCACTACCCTGTTCCACGGGCTGGATGACCGGCTTCTCAAGGAGCTGGAG
NM_080431.4       CTGTCGGGGGGCACTACCCTGTTCCACGGGCTGGATGACCGGCTTCTCAAGGAGCTGGAG
                  ************************************************************

HsCD00043277      CAGCTGGCCTCCAAGGACACCCCCATCAAGATCACGGCTCCCCCCGACCGGTGGTTCTCC
NM_080431.4       CAGCTGGCCTCCAAGGACACCCCCATCAAGATCACGGCTCCCCCCGACCGGTGGTTCTCC
                  ************************************************************

HsCD00043277      ACCTGGATTGGAGCCTCCATCGTCACCTCTCTGAGTAGCTTCAAGCAGATGTGGGTCACC
NM_080431.4       ACCTGGATTGGAGCCTCCATCGTCACCTCTCTGAGTAGCTTCAAGCAGATGTGGGTCACC
                  ************************************************************

HsCD00043277      GCCGCAGACTTCAAGGAGTTTGGGACCTCCGTGGTGCAGAGAAGATGCTTCTAG
NM_080431.4       GCCGCAGACTTCAAGGAGTTTGGGACCTCCGTGGTGCAGAGAAGATGCTTCTGA
                  ****************************************************..

Protein

SMART domain : ACTIN : Actin
UniProt : Q96LK1
UniProt : Q8TDY3
HPRD : 12254

Clone

Cloning Information : 170511
HIP Master Clone ID : 143977
Original Clone ID : FLH170511.01X

Organism


TAX_ID : 9606
Species Specific ID: 140625

Genome

Chromosome : 1
Map Location : 1p36.32
Ensembl : ENSG00000169717

Reagents

Labome : ACTRT2-antibody