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ACTR10 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTR10
Gene Name:  actin-related protein 10 homolog (S. cerevisiae)
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH170815.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00043311

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1254nts        
Open reading frame : 1 to 1254
Reference Sequence Alignment

ATGCCGCTCTACGAGGGCCTGGGGAGCGGCGGGGAGAAGACGGCGGTCGTGATCGACCTG
GGAGAGGCCTTTACCAAGTGTGGATTTGCTGGAGAAACTGGTCCAAGATGTATAATTCCT
AGTGTGATAAAAAGAGCTGGGATGCCTAAGCCTGTCAGAGTTGTTCAGTATAATATCAAT
ACAGAAGAATTATATTCCTACCTAAAGGAATTCATCCACATACTATATTTCAGGCATCTA
TTGGTGAATCCCAGAGACCGCCGAGTTGTGATTATCGAATCGGTATTATGTCCTTCTCAC
TTCAGAGAGACACTCACTCGTGTTCTTTTCAAATATTTTGAGGTTCCATCTGTCTTGCTT
GCTCCAAGTCATCTAATGGCTCTTCTGACGCTTGGAATTAATTCTGCCATGGTCCTAGAT
TGTGGATATAGGGAAAGCCTGGTGTTACCCATATATGAAGGAATCCCAGTTCTAAATTGT
TGGGGAGCACTACCCCTAGGAGGAAAAGCTCTTCACAAAGAGTTGGAAACTCAACTATTG
GAACAATGTACTGTTGACACAAGTGTTGCTAAAGAACAGAGCCTTCCCTCAGTGATGGGT
TCAGTTCCGGAAGGTGTCTTAGAGGACATTAAAGCGCGTACTTGCTTTGTAAGTGATCTG
AAGCGAGGACTAAAAATCCAAGCAGCAAAATTTAATATTGATGGGAATAATGAGCGTCCC
TCCCCACCCCCAAATGTTGACTATCCATTAGATGGAGAGAAGATTTTACATATCCTTGGA
TCAATCAGAGATTCAGTTGTGGAAATTCTTTTTGAACAAGATAATGAAGAGCAATCAGTT
GCCACTTTAATATTGGATTCCCTTATACAGTGTCCGATAGACACCAGGAAGCAACTAGCA
GAGAATTTGGTAGTCATAGGTGGCACTTCTATGTTGCCAGGATTTCTCCACAGATTGCTT
GCAGAAATAAGGTATTTGGTAGAAAAACCAAAATATAAAAAAGCACTTGGCACTAAGACA
TTTCGAATTCATACTCCACCTGCAAAAGCTAATTGTGTGGCCTGGTTGGGAGGGGCTATT
TTTGGAGCATTACAAGATATACTTGGGAGCCGTTCTGTTTCAAAGGAATATTATAATCAG
ACGGGCCGTATACCTGATTGGTGTTCTCTCAATAACCCACCTTTGGAAATGATGTTTGAT
GTCGGGAAAACTCAACCACCTCTGATGAAGAGAGCATTTTCCACTGAGAAATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1291
Start on reference sequence 38
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site U19 primer 517 535
primer site Att P2 Primer 3026 3043
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTR10
Symbol Nomenclature : ACTR10
SYNONYM : ACTR11; Arp11; HARP11
Designation : actin-related protein 10|actin-related protein 11
Full Nomenclature : actin-related protein 10 homolog (S. cerevisiae)
GENEID : 55860
HGNC : 17372
Target GenBank: BC011997

 

Reference Sequence Alignment

  

HsCD00043311      ATGCCGCTCTACGAGGGCCTGGGGAGCGGCGGGGAGAAGACGGCGGTCGTGATCGACCTG
NM_018477.2       ATGCCGCTCTACGAGGGCCTGGGGAGCGGCGGGGAGAAGACGGCGGTCGTGATCGACCTG
                  ************************************************************

HsCD00043311      GGAGAGGCCTTTACCAAGTGTGGATTTGCTGGAGAAACTGGTCCAAGATGTATAATTCCT
NM_018477.2       GGAGAGGCCTTTACCAAGTGTGGATTTGCTGGAGAAACTGGTCCAAGATGTATAATTCCT
                  ************************************************************

HsCD00043311      AGTGTGATAAAAAGAGCTGGGATGCCTAAGCCTGTCAGAGTTGTTCAGTATAATATCAAT
NM_018477.2       AGTGTGATAAAAAGAGCTGGGATGCCTAAGCCTGTCAGAGTTGTTCAGTATAATATCAAT
                  ************************************************************

HsCD00043311      ACAGAAGAATTATATTCCTACCTAAAGGAATTCATCCACATACTATATTTCAGGCATCTA
NM_018477.2       ACAGAAGAATTATATTCCTACCTAAAGGAATTCATCCACATACTATATTTCAGGCATCTA
                  ************************************************************

HsCD00043311      TTGGTGAATCCCAGAGACCGCCGAGTTGTGATTATCGAATCGGTATTATGTCCTTCTCAC
NM_018477.2       TTGGTGAATCCCAGAGACCGCCGAGTTGTGATTATCGAATCGGTATTATGTCCTTCTCAC
                  ************************************************************

HsCD00043311      TTCAGAGAGACACTCACTCGTGTTCTTTTCAAATATTTTGAGGTTCCATCTGTCTTGCTT
NM_018477.2       TTCAGAGAGACACTCACTCGTGTTCTTTTCAAATATTTTGAGGTTCCATCTGTCTTGCTT
                  ************************************************************

HsCD00043311      GCTCCAAGTCATCTAATGGCTCTTCTGACGCTTGGAATTAATTCTGCCATGGTCCTAGAT
NM_018477.2       GCTCCAAGTCATCTAATGGCTCTTCTGACGCTTGGAATTAATTCTGCCATGGTCCTAGAT
                  ************************************************************

HsCD00043311      TGTGGATATAGGGAAAGCCTGGTGTTACCCATATATGAAGGAATCCCAGTTCTAAATTGT
NM_018477.2       TGTGGATATAGGGAAAGCCTGGTGTTACCCATATATGAAGGAATCCCAGTTCTAAATTGT
                  ************************************************************

HsCD00043311      TGGGGAGCACTACCCCTAGGAGGAAAAGCTCTTCACAAAGAGTTGGAAACTCAACTATTG
NM_018477.2       TGGGGAGCACTACCCCTAGGAGGAAAAGCTCTTCACAAAGAGTTGGAAACTCAACTATTG
                  ************************************************************

HsCD00043311      GAACAATGTACTGTTGACACAAGTGTTGCTAAAGAACAGAGCCTTCCCTCAGTGATGGGT
NM_018477.2       GAACAATGTACTGTTGACACAAGTGTTGCTAAAGAACAGAGCCTTCCCTCAGTGATGGGT
                  ************************************************************

HsCD00043311      TCAGTTCCGGAAGGTGTCTTAGAGGACATTAAAGCGCGTACTTGCTTTGTAAGTGATCTG
NM_018477.2       TCAGTTCCGGAAGGTGTCTTAGAGGACATTAAAGCGCGTACTTGCTTTGTAAGTGATCTG
                  ************************************************************

HsCD00043311      AAGCGAGGACTAAAAATCCAAGCAGCAAAATTTAATATTGATGGGAATAATGAGCGTCCC
NM_018477.2       AAGCGAGGACTAAAAATCCAAGCAGCAAAATTTAATATTGATGGGAATAATGAGCGTCCC
                  ************************************************************

HsCD00043311      TCCCCACCCCCAAATGTTGACTATCCATTAGATGGAGAGAAGATTTTACATATCCTTGGA
NM_018477.2       TCCCCACCCCCAAATGTTGACTATCCATTAGATGGAGAGAAGATTTTACATATCCTTGGA
                  ************************************************************

HsCD00043311      TCAATCAGAGATTCAGTTGTGGAAATTCTTTTTGAACAAGATAATGAAGAGCAATCAGTT
NM_018477.2       TCAATCAGAGATTCAGTTGTGGAAATTCTTTTTGAACAAGATAATGAAGAGCAATCAGTT
                  ************************************************************

HsCD00043311      GCCACTTTAATATTGGATTCCCTTATACAGTGTCCGATAGACACCAGGAAGCAACTAGCA
NM_018477.2       GCCACTTTAATATTGGATTCCCTTATACAGTGTCCGATAGACACCAGGAAGCAACTAGCA
                  ************************************************************

HsCD00043311      GAGAATTTGGTAGTCATAGGTGGCACTTCTATGTTGCCAGGATTTCTCCACAGATTGCTT
NM_018477.2       GAGAATTTGGTAGTCATAGGTGGCACTTCTATGTTGCCAGGATTTCTCCACAGATTGCTT
                  ************************************************************

HsCD00043311      GCAGAAATAAGGTATTTGGTAGAAAAACCAAAATATAAAAAAGCACTTGGCACTAAGACA
NM_018477.2       GCAGAAATAAGGTATTTGGTAGAAAAACCAAAATATAAAAAAGCACTTGGCACTAAGACA
                  ************************************************************

HsCD00043311      TTTCGAATTCATACTCCACCTGCAAAAGCTAATTGTGTGGCCTGGTTGGGAGGGGCTATT
NM_018477.2       TTTCGAATTCATACTCCACCTGCAAAAGCTAATTGTGTGGCCTGGTTGGGAGGGGCTATT
                  ************************************************************

HsCD00043311      TTTGGAGCATTACAAGATATACTTGGGAGCCGTTCTGTTTCAAAGGAATATTATAATCAG
NM_018477.2       TTTGGAGCATTACAAGATATACTTGGGAGCCGTTCTGTTTCAAAGGAATATTATAATCAG
                  ************************************************************

HsCD00043311      ACGGGCCGTATACCTGATTGGTGTTCTCTCAATAACCCACCTTTGGAAATGATGTTTGAT
NM_018477.2       ACGGGCCGTATACCTGATTGGTGTTCTCTCAATAACCCACCTTTGGAAATGATGTTTGAT
                  ************************************************************

HsCD00043311      GTCGGGAAAACTCAACCACCTCTGATGAAGAGAGCATTTTCCACTGAGAAATAG
NM_018477.2       GTCGGGAAAACTCAACCACCTCTGATGAAGAGAGCATTTTCCACTGAGAAATAG
                  ******************************************************

Protein

SMART domain : ACTIN : Actin
UniProt : Q9NZ32
HPRD : 12420

Clone

Cloning Information : 170815
HIP Master Clone ID : 144281
Original Clone ID : FLH170815.01X

Organism


TAX_ID : 9606
Species Specific ID: 55860

Genome

Chromosome : 14
Map Location : 14q23.1

Reagents

Labome : ACTR10-antibody