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TIMM44 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  TIMM44
Gene Name:  translocase of inner mitochondrial membrane 44 homolog (yeast)
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH170901.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00043318

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1359nts         Open reading frame : 1 to 1359
Reference Sequence Alignment

ATGGCGGCGGCGGCCCTGCGGAGTGGCTGGTGCCGCTGTCCACGGAGATGCCTCGGCAGT
GGAATCCAATTTCTTTCCAGCCACAACCTACCCCATGGGTCGACCTATCAGATGCGCCGG
CCGGGCGGAGAGCTGCCACTGTCCAAATCATATTCTTCTGGAAACAGAAAAGGCTTTCTG
TCCGGCTTGCTAGATAATGTCAAACAAGAATTAGCCAAAAACAAAGAAATGAAAGAAAGT
ATAAAAAAATTCCGTGACGAGGCCAGAAGGCTAGAAGAATCAGACGTGCTCCAGGAGGCC
AGAAGGAAATACAAAACCATCGAGTCAGAAACCGTGCGGACGAGCGAGGTGCTACGGAAG
AAGCTTGGGGAGCTGACGGGCACCGTGAAGGAGAGCCTTCACGAAGTCAGTAAAAGTGAT
CTCGGCCGGAAAATCAAGGAGGGCGTGGAGGAAGCAGCCAAGACGGCCAAGCAGTCGGCC
GAGTCGGTATCCAAAGGCGGGGAGAAGCTGGGCAGGACAGCGGCCTTCAGAGCCCTCTCC
CAGGGGGTGGAGTCCGTGAAGAAGGAAATTGACGACAGCGTCCTGGGACAGACCGGGCCC
TACCGGAGGCCCCAGCGACTCCGGAAGAGAACGGAGTTTGCGGGAGATAAGTTCAAGGAG
GAGAAAGTGTTTGAGCCAAACGAGGAGGCCCTGGGGGTCGTGCTGCACAAGGACTCCAAG
TGGTACCAGCAGTGGAAGGACTTCAAGGAGAACAACGTGGTGTTTAACCGGTTCTTCGAG
ATGAAGATGAAGTATGACGAAAGCGACAACGCGTTCATCCGGGCATCCCGGGCCCTTACG
GACAAGGTCACCGACTTGCTGGGGGGCCTGTTCTCCAAGACAGAGATGTCGGAGGTGCTC
ACGGAGATCCTCCGGGTGGACCCGGCCTTTGACAAGGACCGGTTTCTGAAACAGTGCGAG
AACGACATCATCCCCAATGTCCTGGAGGCCATGATTTCTGGAGAGCTTGACATTCTCAAA
GACTGGTGCTATGAAGCTACTTACAGCCAGCTGGCCCACCCCATCCAGCAGGCCAAGGCA
CTGGGTCTCCAGTTCCATTCTCGCATCCTAGACATTGACAACGTCGACCTGGCCATGGGC
AAGATGATGGAGCAGGGGCCGGTGCTGATCATCACCTTCCAGGCACAGCTGGTGATGGTG
GTCAGGAACCCCAAAGGCGAGGTGGTGGAGGGTGACCCGGACAAGGTGCTGCGGATGCTG
TACGTGTGGGCGCTCTGCCGAGACCAGGACGAGCTCAACCCCTACGCGGCCTGGCGGCTC
CTGGACATCTCGGCCTCCAGCACCGAGCAGATTCTCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1361
Start on reference sequence 3
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TIMM44
Symbol Nomenclature : TIMM44
SYNONYM : TIM44
Designation : mitochondrial import inner membrane translocase subunit TIM44|mitochondrial inner membrane translocase
Full Nomenclature : translocase of inner mitochondrial membrane 44 homolog (yeast)
GENEID : 10469
HGNC : 17316
MIM : 605058
Vega : OTTHUMG00000182478
Target GenBank: BC033628

 

Reference Sequence Alignment

  

HsCD00043318      ATGGCGGCGGCGGCCCTGCGGAGTGGCTGGTGCCGCTGTCCACGGAGATGCCTCGGCAGT
NM_006351.3       ATGGCGGCGGCGGCCCTGCGGAGTGGCTGGTGCCGCTGTCCACGGAGATGCCTCGGCAGT
                  ************************************************************

HsCD00043318      GGAATCCAATTTCTTTCCAGCCACAACCTACCCCATGGGTCGACCTATCAGATGCGCCGG
NM_006351.3       GGAATCCAATTTCTTTCCAGCCACAACCTACCCCATGGGTCGACCTATCAGATGCGCCGG
                  ************************************************************

HsCD00043318      CCGGGCGGAGAGCTGCCACTGTCCAAATCATATTCTTCTGGAAACAGAAAAGGCTTTCTG
NM_006351.3       CCGGGCGGAGAGCTGCCACTGTCCAAATCATATTCTTCTGGAAACAGAAAAGGCTTTCTG
                  ************************************************************

HsCD00043318      TCCGGCTTGCTAGATAATGTCAAACAAGAATTAGCCAAAAACAAAGAAATGAAAGAAAGT
NM_006351.3       TCCGGCTTGCTAGATAATGTCAAACAAGAATTAGCCAAAAACAAAGAAATGAAAGAAAGT
                  ************************************************************

HsCD00043318      ATAAAAAAATTCCGTGACGAGGCCAGAAGGCTAGAAGAATCAGACGTGCTCCAGGAGGCC
NM_006351.3       ATAAAAAAATTCCGTGACGAGGCCAGAAGGCTAGAAGAATCAGACGTGCTCCAGGAGGCC
                  ************************************************************

HsCD00043318      AGAAGGAAATACAAAACCATCGAGTCAGAAACCGTGCGGACGAGCGAGGTGCTACGGAAG
NM_006351.3       AGAAGGAAATACAAAACCATCGAGTCAGAAACCGTGCGGACGAGCGAGGTGCTACGGAAG
                  ************************************************************

HsCD00043318      AAGCTTGGGGAGCTGACGGGCACCGTGAAGGAGAGCCTTCACGAAGTCAGTAAAAGTGAT
NM_006351.3       AAGCTTGGGGAGCTGACGGGCACCGTGAAGGAGAGCCTTCACGAAGTCAGTAAAAGTGAT
                  ************************************************************

HsCD00043318      CTCGGCCGGAAAATCAAGGAGGGCGTGGAGGAAGCAGCCAAGACGGCCAAGCAGTCGGCC
NM_006351.3       CTCGGCCGGAAAATCAAGGAGGGCGTGGAGGAAGCAGCCAAGACGGCCAAGCAGTCGGCC
                  ************************************************************

HsCD00043318      GAGTCGGTATCCAAAGGCGGGGAGAAGCTGGGCAGGACAGCGGCCTTCAGAGCCCTCTCC
NM_006351.3       GAGTCGGTATCCAAAGGCGGGGAGAAGCTGGGCAGGACAGCGGCCTTCAGAGCCCTCTCC
                  ************************************************************

HsCD00043318      CAGGGGGTGGAGTCCGTGAAGAAGGAAATTGACGACAGCGTCCTGGGACAGACCGGGCCC
NM_006351.3       CAGGGGGTGGAGTCCGTGAAGAAGGAAATTGACGACAGCGTCCTGGGACAGACCGGGCCC
                  ************************************************************

HsCD00043318      TACCGGAGGCCCCAGCGACTCCGGAAGAGAACGGAGTTTGCGGGAGATAAGTTCAAGGAG
NM_006351.3       TACCGGAGGCCCCAGCGACTCCGGAAGAGAACGGAGTTTGCGGGAGATAAGTTCAAGGAG
                  ************************************************************

HsCD00043318      GAGAAAGTGTTTGAGCCAAACGAGGAGGCCCTGGGGGTCGTGCTGCACAAGGACTCCAAG
NM_006351.3       GAGAAAGTGTTTGAGCCAAACGAGGAGGCCCTGGGGGTCGTGCTGCACAAGGACTCCAAG
                  ************************************************************

HsCD00043318      TGGTACCAGCAGTGGAAGGACTTCAAGGAGAACAACGTGGTGTTTAACCGGTTCTTCGAG
NM_006351.3       TGGTACCAGCAGTGGAAGGACTTCAAGGAGAACAACGTGGTGTTTAACCGGTTCTTCGAG
                  ************************************************************

HsCD00043318      ATGAAGATGAAGTATGACGAAAGCGACAACGCGTTCATCCGGGCATCCCGGGCCCTTACG
NM_006351.3       ATGAAGATGAAGTATGACGAAAGCGACAACGCGTTCATCCGGGCATCCCGGGCCCTTACG
                  ************************************************************

HsCD00043318      GACAAGGTCACCGACTTGCTGGGGGGCCTGTTCTCCAAGACAGAGATGTCGGAGGTGCTC
NM_006351.3       GACAAGGTCACCGACTTGCTGGGGGGCCTGTTCTCCAAGACAGAGATGTCGGAGGTGCTC
                  ************************************************************

HsCD00043318      ACGGAGATCCTCCGGGTGGACCCGGCCTTTGACAAGGACCGGTTTCTGAAACAGTGCGAG
NM_006351.3       ACGGAGATCCTCCGGGTGGACCCGGCCTTTGACAAGGACCGGTTTCTGAAACAGTGCGAG
                  ************************************************************

HsCD00043318      AACGACATCATCCCCAATGTCCTGGAGGCCATGATTTCTGGAGAGCTTGACATTCTCAAA
NM_006351.3       AACGACATCATCCCCAATGTCCTGGAGGCCATGATTTCTGGAGAGCTTGACATTCTCAAA
                  ************************************************************

HsCD00043318      GACTGGTGCTATGAAGCTACTTACAGCCAGCTGGCCCACCCCATCCAGCAGGCCAAGGCA
NM_006351.3       GACTGGTGCTATGAAGCTACTTACAGCCAGCTGGCCCACCCCATCCAGCAGGCCAAGGCA
                  ************************************************************

HsCD00043318      CTGGGTCTCCAGTTCCATTCTCGCATCCTAGACATTGACAACGTCGACCTGGCCATGGGC
NM_006351.3       CTGGGTCTCCAGTTCCATTCTCGCATCCTAGACATTGACAACGTCGACCTGGCCATGGGC
                  ************************************************************

HsCD00043318      AAGATGATGGAGCAGGGGCCGGTGCTGATCATCACCTTCCAGGCACAGCTGGTGATGGTG
NM_006351.3       AAGATGATGGAGCAGGGGCCGGTGCTGATCATCACCTTCCAGGCACAGCTGGTGATGGTG
                  ************************************************************

HsCD00043318      GTCAGGAACCCCAAAGGCGAGGTGGTGGAGGGTGACCCGGACAAGGTGCTGCGGATGCTG
NM_006351.3       GTCAGGAACCCCAAAGGCGAGGTGGTGGAGGGTGACCCGGACAAGGTGCTGCGGATGCTG
                  ************************************************************

HsCD00043318      TACGTGTGGGCGCTCTGCCGAGACCAGGACGAGCTCAACCCCTACGCGGCCTGGCGGCTC
NM_006351.3       TACGTGTGGGCGCTCTGCCGAGACCAGGACGAGCTCAACCCCTACGCGGCCTGGCGGCTC
                  ************************************************************

HsCD00043318      CTGGACATCTCGGCCTCCAGCACCGAGCAGATTCTCTAG
NM_006351.3       CTGGACATCTCGGCCTCCAGCACCGAGCAGATTCTCTGA
                  *************************************..

Pathways

Reactome : Metabolism of proteins
Reactome : Mitochondrial protein import

Protein

SMART domain : Tim44 :
UniProt : O43615
HPRD : 05450

Clone

Cloning Information : 170901
HIP Master Clone ID : 144367
Original Clone ID : FLH170901.01X

Organism


TAX_ID : 9606
Species Specific ID: 10469

Genome

Chromosome : 19
Map Location : 19p13.3-p13.2
Ensembl : ENSG00000104980

Reagents

Labome : TIMM44-antibody