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GJB4 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GJB4
Gene Name:  gap junction protein, beta 4, 30.3kDa
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH171092.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00043341

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 801nts         Open reading frame : 1 to 801
Reference Sequence Alignment

ATGAACTGGGCATTTCTGCAGGGCCTGCTGAGTGGCGTGAACAAGTACTCCACAGTGCTG
AGCCGCATCTGGCTGTCTGTGGTGTTCATCTTTCGTGTGCTGGTGTACGTGGTGGCAGCG
GAGGAGGTGTGGGACGATGAGCAGAAGGACTTTGTCTGCAACACCAAGCAGCCCGGCTGC
CCCAACGTCTGCTATGACGAGTTCTTCCCCGTGTCCCACGTGCGCCTCTGGGCCCTACAG
CTCATCCTGGTCACGTGCCCCTCACTGCTCGTGGTCATGCACGTGGCCTACCGCGAGGAA
CGCGAGCGCAAGCACCACCTGAAACACGGGCCCAATGCCCCGTCCCTGTACGACAACCTG
AGCAAGAAGCGGGGCGGACTGTGGTGGACGTACTTGCTGAGCCTCATCTTCAAGGCCGCC
GTGGATGCTGGCTTCCTCTATATCTTCCACCGCCTCTACAAGGATTATGACATGCCCCGC
GTGGTGGCCTGCTCCGTGGAGCCTTGCCCCCACACTGTGGACTGTTACATCTCCCGGCCC
ACGGAGAAGAAGGTCTTCACCTACTTCATGGTGACCACAGCTGCCATCTGCATCCTGCTC
AACCTCAGTGAAGTCTTCTACCTGGTGGGCAAGAGGTGCATGGAGATCTTCGGCCCCAGG
CACCGGCGGCCTCGGTGCCGGGAATGCCTACCCGATACGTGCCCACCATATGTCCTCTCC
CAGGGAGGGCACCCTGAGGATGGGAACTCTGTCCTAATGAAGGCTGGGTCGGCCCCAGTG
GATGCAGGTGGGTATCCATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 879
Start on reference sequence 79
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GJB4
Symbol Nomenclature : GJB4
SYNONYM : CX30.3; EKV
Designation : connexin 30.3|connexin-30.3|gap junction beta-4 protein
Full Nomenclature : gap junction protein, beta 4, 30.3kDa
GENEID : 127534
HGNC : 4286
MIM : 605425
Vega : OTTHUMG00000004052
Target GenBank: BC034709

 

Reference Sequence Alignment

  

HsCD00043341      ATGAACTGGGCATTTCTGCAGGGCCTGCTGAGTGGCGTGAACAAGTACTCCACAGTGCTG
NM_153212.2       ATGAACTGGGCATTTCTGCAGGGCCTGCTGAGTGGCGTGAACAAGTACTCCACAGTGCTG
                  ************************************************************

HsCD00043341      AGCCGCATCTGGCTGTCTGTGGTGTTCATCTTTCGTGTGCTGGTGTACGTGGTGGCAGCG
NM_153212.2       AGCCGCATCTGGCTGTCTGTGGTGTTCATCTTTCGTGTGCTGGTGTACGTGGTGGCAGCG
                  ************************************************************

HsCD00043341      GAGGAGGTGTGGGACGATGAGCAGAAGGACTTTGTCTGCAACACCAAGCAGCCCGGCTGC
NM_153212.2       GAGGAGGTGTGGGACGATGAGCAGAAGGACTTTGTCTGCAACACCAAGCAGCCCGGCTGC
                  ************************************************************

HsCD00043341      CCCAACGTCTGCTATGACGAGTTCTTCCCCGTGTCCCACGTGCGCCTCTGGGCCCTACAG
NM_153212.2       CCCAACGTCTGCTATGACGAGTTCTTCCCCGTGTCCCACGTGCGCCTCTGGGCCCTACAG
                  ************************************************************

HsCD00043341      CTCATCCTGGTCACGTGCCCCTCACTGCTCGTGGTCATGCACGTGGCCTACCGCGAGGAA
NM_153212.2       CTCATCCTGGTCACGTGCCCCTCACTGCTCGTGGTCATGCACGTGGCCTACCGCGAGGAA
                  ************************************************************

HsCD00043341      CGCGAGCGCAAGCACCACCTGAAACACGGGCCCAATGCCCCGTCCCTGTACGACAACCTG
NM_153212.2       CGCGAGCGCAAGCACCACCTGAAACACGGGCCCAATGCCCCGTCCCTGTACGACAACCTG
                  ************************************************************

HsCD00043341      AGCAAGAAGCGGGGCGGACTGTGGTGGACGTACTTGCTGAGCCTCATCTTCAAGGCCGCC
NM_153212.2       AGCAAGAAGCGGGGCGGACTGTGGTGGACGTACTTGCTGAGCCTCATCTTCAAGGCCGCC
                  ************************************************************

HsCD00043341      GTGGATGCTGGCTTCCTCTATATCTTCCACCGCCTCTACAAGGATTATGACATGCCCCGC
NM_153212.2       GTGGATGCTGGCTTCCTCTATATCTTCCACCGCCTCTACAAGGATTATGACATGCCCCGC
                  ************************************************************

HsCD00043341      GTGGTGGCCTGCTCCGTGGAGCCTTGCCCCCACACTGTGGACTGTTACATCTCCCGGCCC
NM_153212.2       GTGGTGGCCTGCTCCGTGGAGCCTTGCCCCCACACTGTGGACTGTTACATCTCCCGGCCC
                  ************************************************************

HsCD00043341      ACGGAGAAGAAGGTCTTCACCTACTTCATGGTGACCACAGCTGCCATCTGCATCCTGCTC
NM_153212.2       ACGGAGAAGAAGGTCTTCACCTACTTCATGGTGACCACAGCTGCCATCTGCATCCTGCTC
                  ************************************************************

HsCD00043341      AACCTCAGTGAAGTCTTCTACCTGGTGGGCAAGAGGTGCATGGAGATCTTCGGCCCCAGG
NM_153212.2       AACCTCAGTGAAGTCTTCTACCTGGTGGGCAAGAGGTGCATGGAGATCTTCGGCCCCAGG
                  ************************************************************

HsCD00043341      CACCGGCGGCCTCGGTGCCGGGAATGCCTACCCGATACGTGCCCACCATATGTCCTCTCC
NM_153212.2       CACCGGCGGCCTCGGTGCCGGGAATGCCTACCCGATACGTGCCCACCATATGTCCTCTCC
                  ************************************************************

HsCD00043341      CAGGGAGGGCACCCTGAGGATGGGAACTCTGTCCTAATGAAGGCTGGGTCGGCCCCAGTG
NM_153212.2       CAGGGAGGGCACCCTGAGGATGGGAACTCTGTCCTAATGAAGGCTGGGTCGGCCCCAGTG
                  ************************************************************

HsCD00043341      GATGCAGGTGGGTATCCATAG
NM_153212.2       GATGCAGGTGGGTATCCATAA
                  ********************.

Pathways

Reactome : Gap junction assembly
Reactome : Gap junction trafficking
Reactome : Gap junction trafficking and regulation
Reactome : Membrane Trafficking
WikiPathway : Calcium Regulation in the Cardiac Cell

Clone

Cloning Information : 171092
HIP Master Clone ID : 144558
Original Clone ID : FLH171092.01X

Organism


TAX_ID : 9606
Species Specific ID: 127534

Genome

Chromosome : 1
Map Location : 1p34.3
Ensembl : ENSG00000189433

Reagents

Labome : GJB4-antibody