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NDUFV1 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  NDUFV1
Gene Name:  NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa
Sequence             
Original Clone ID: ccsbBroad304_01075
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00434184

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1393nts         Open reading frame : 1 to 1393
Reference Sequence Alignment

ATGCTGGCAACACGGCGGCTGCTCGGCTGGTCGCTTCCCGCGCGGGTATCTGTGCGTTTC
AGCGGCGACACGACAGCACCCAAGAAAACCTCATTTGGCTCGCTGAAGGATGAAGACCGG
ATTTTCACCAACCTGTACGGCCGCCATGACTGGAGGCTGAAAGGTTCCCTGAGTCGAGGT
GACTGGTACAAGACAAAGGAGATCCTGCTGAAGGGGCCCGACTGGATCCTGGGCGAGATC
AAGACATCGGGTTTGAGGGGCCGTGGAGGCGCTGGCTTCCCCACTGGCCTCAAGTGGAGC
TTCATGAATAAGCCCTCAGATGGCAGGCCCAAGTATCTGGTGGTGAACGCAGACGAGGGG
GAGCCGGGCACCTGCAAGGACCGGGAGATCTTACGCCATGATCCTCACAAGCTGCTGGAA
GGCTGCCTGGTGGGGGGCCGGGCCATGGGCGCCCGCGCTGCCTATATCTACATCCGAGGG
GAATTCTACAATGAGGCCTCCAATCTGCAGGTGGCCATCCGAGAGGCCTATGAGGCAGGT
CTGATTGGCAAGAATGCTTGTGGCTCTGGCTATGATTTTGACGTGTTTGTGGTGCGCGGG
GCTGGGGCCTACATCTGTGGAGAGGAGACAGCGCTCATCGAGTCCATTGAGGGCAAGCAG
GGCAAGCCCCGCCTGAAGCCCCCCTTCCCCGCAGACGTGGGAGTGTTTGGCTGCCCCACA
ACTGTGGCCAACGTGGAGACAGTGGCAGTGTCCCCCACAATCTGCCGCCGTGGAGGTACC
TGGTTTGCTGGCTTTGGCAGAGAACGCAACTCAGGCACCAAACTATTCAACATCTCTGGC
CATGTCAACCACCCTTGCACTGTGGAGGAGGAGATGTCTGTGCCCTTGAAAGAACTGATT
GAGAAGCATGCTGGGGGTGTCACGGGCGGCTGGGACAACCTCCTTGCTGTGATCCCTGGC
GGCTCGTCTACCCCACTGATCCCCAAGTCTGTGTGTGAGACGGTGCTGATGGACTTCGAT
GCGCTGGTGCAGGCACAGACAGGCCTGGGCACAGCTGCGGTGATCGTCATGGACCGCTCG
ACGGACATCGTGAAAGCCATCGCCCGCCTCATTGAGTTCTATAAGCACGAGAGCTGTGGC
CAGTGTACCCCATGCCGTGAGGGTGTGGACTGGATGAACAAGGTGATGGCACGTTTCGTG
AGGGGGGATGCCCGGCCGGCCGAGATCGACTCCCTGTGGGAGATCAGCAAGCAGATAGAA
GGCCATACGATTTGTGCTCTGGGTGACGGGGCCGCCTGGCCTGTGCAGGGTCTGATCCGC
CACTTTCGGCCGGAGCTCGAGGAGCGGATGCAGCGGTTTGCCCAGCAGCATCAGGCCCGG
CAGGCTGCCTCT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NDUFV1
Symbol Nomenclature : NDUFV1
SYNONYM : CI-51K; CI51KD; UQOR1
Designation : NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial|NADH-ubiquinone oxidoreductase 51 kDa subunit|complex I 51 kda subunit|complex I 51kDa subunit|complex I, mitochondrial respiratory chain|mitochondrial NADH dehydrogenase ubiquinone flavoprotein 1|mitochondrial NADH:ubiquinone oxidoreductase 51 kda subunit
Full Nomenclature : NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa
GENEID : 4723
GenBank Accession : DQ896566
HGNC : 7716
MIM : 161015
Vega : OTTHUMG00000166215
Target GenBank: DQ896566

 

Reference Sequence Alignment

  

HsCD00434184        ATGCTGGCAACACGGCGGCTGCTCGGCTGGTCGCTTCCCGCGCGGGTATCTGTGCGTTTC
NM_007103.3         ATGCTGGCAACACGGCGGCTGCTCGGCTGGTCGCTTCCCGCGCGGGTATCTGTGCGTTTC
NM_001166102.1      ATGCTGGCAACACGGCGGCTGCTCGGCTGGTCGCTTCCCGCGCGG---------------
                    *********************************************               

HsCD00434184        AGCGGCGACACGACAGCACCCAAGAAAACCTCATTTGGCTCGCTGAAGGATGAAGACCGG
NM_007103.3         AGCGGCGACACGACAGCACCCAAGAAAACCTCATTTGGCTCGCTGAAGGATGAAGACCGG
NM_001166102.1      ------------ACAGCACCCAAGAAAACCTCATTTGGCTCGCTGAAGGATGAAGACCGG
                                ************************************************

HsCD00434184        ATTTTCACCAACCTGTACGGCCGCCATGACTGGAGGCTGAAAGGTTCCCTGAGTCGAGGT
NM_007103.3         ATTTTCACCAACCTGTACGGCCGCCATGACTGGAGGCTGAAAGGTTCCCTGAGTCGAGGT
NM_001166102.1      ATTTTCACCAACCTGTACGGCCGCCATGACTGGAGGCTGAAAGGTTCCCTGAGTCGAGGT
                    ************************************************************

HsCD00434184        GACTGGTACAAGACAAAGGAGATCCTGCTGAAGGGGCCCGACTGGATCCTGGGCGAGATC
NM_007103.3         GACTGGTACAAGACAAAGGAGATCCTGCTGAAGGGGCCCGACTGGATCCTGGGCGAGATC
NM_001166102.1      GACTGGTACAAGACAAAGGAGATCCTGCTGAAGGGGCCCGACTGGATCCTGGGCGAGATC
                    ************************************************************

HsCD00434184        AAGACATCGGGTTTGAGGGGCCGTGGAGGCGCTGGCTTCCCCACTGGCCTCAAGTGGAGC
NM_007103.3         AAGACATCGGGTTTGAGGGGCCGTGGAGGCGCTGGCTTCCCCACTGGCCTCAAGTGGAGC
NM_001166102.1      AAGACATCGGGTTTGAGGGGCCGTGGAGGCGCTGGCTTCCCCACTGGCCTCAAGTGGAGC
                    ************************************************************

HsCD00434184        TTCATGAATAAGCCCTCAGATGGCAGGCCCAAGTATCTGGTGGTGAACGCAGACGAGGGG
NM_007103.3         TTCATGAATAAGCCCTCAGATGGCAGGCCCAAGTATCTGGTGGTGAACGCAGACGAGGGG
NM_001166102.1      TTCATGAATAAGCCCTCAGATGGCAGGCCCAAGTATCTGGTGGTGAACGCAGACGAGGGG
                    ************************************************************

HsCD00434184        GAGCCGGGCACCTGCAAGGACCGGGAGATCTTACGCCATGATCCTCACAAGCTGCTGGAA
NM_007103.3         GAGCCGGGCACCTGCAAGGACCGGGAGATCTTACGCCATGATCCTCACAAGCTGCTGGAA
NM_001166102.1      GAGCCGGGCACCTGCAAGGACCGGGAGATCTTACGCCATGATCCTCACAAGCTGCTGGAA
                    ************************************************************

HsCD00434184        GGCTGCCTGGTGGGGGGCCGGGCCATGGGCGCCCGCGCTGCCTATATCTACATCCGAGGG
NM_007103.3         GGCTGCCTGGTGGGGGGCCGGGCCATGGGCGCCCGCGCTGCCTATATCTACATCCGAGGG
NM_001166102.1      GGCTGCCTGGTGGGGGGCCGGGCCATGGGCGCCCGCGCTGCCTATATCTACATCCGAGGG
                    ************************************************************

HsCD00434184        GAATTCTACAATGAGGCCTCCAATCTGCAGGTGGCCATCCGAGAGGCCTATGAGGCAGGT
NM_007103.3         GAATTCTACAATGAGGCCTCCAATCTGCAGGTGGCCATCCGAGAGGCCTATGAGGCAGGT
NM_001166102.1      GAATTCTACAATGAGGCCTCCAATCTGCAGGTGGCCATCCGAGAGGCCTATGAGGCAGGT
                    ************************************************************

HsCD00434184        CTGATTGGCAAGAATGCTTGTGGCTCTGGCTATGATTTTGACGTGTTTGTGGTGCGCGGG
NM_007103.3         CTGATTGGCAAGAATGCTTGTGGCTCTGGCTATGATTTTGACGTGTTTGTGGTGCGCGGG
NM_001166102.1      CTGATTGGCAAGAATGCTTGTGGCTCTGGCTATGATTTTGACGTGTTTGTGGTGCGCGGG
                    ************************************************************

HsCD00434184        GCTGGGGCCTACATCTGTGGAGAGGAGACAGCGCTCATCGAGTCCATTGAGGGCAAGCAG
NM_007103.3         GCTGGGGCCTACATCTGTGGAGAGGAGACAGCGCTCATCGAGTCCATTGAGGGCAAGCAG
NM_001166102.1      GCTGGGGCCTACATCTGTGGAGAGGAGACAGCGCTCATCGAGTCCATTGAGGGCAAGCAG
                    ************************************************************

HsCD00434184        GGCAAGCCCCGCCTGAAGCCCCCCTTCCCCGCAGACGTGGGAGTGTTTGGCTGCCCCACA
NM_007103.3         GGCAAGCCCCGCCTGAAGCCCCCCTTCCCCGCAGACGTGGGAGTGTTTGGCTGCCCCACA
NM_001166102.1      GGCAAGCCCCGCCTGAAGCCCCCCTTCCCCGCAGACGTGGGAGTGTTTGGCTGCCCCACA
                    ************************************************************

HsCD00434184        ACTGTGGCCAACGTGGAGACAGTGGCAGTGTCCCCCACAATCTGCCGCCGTGGAGGTACC
NM_007103.3         ACTGTGGCCAACGTGGAGACAGTGGCAGTGTCCCCCACAATCTGCCGCCGTGGAGGTACC
NM_001166102.1      ACTGTGGCCAACGTGGAGACAGTGGCAGTGTCCCCCACAATCTGCCGCCGTGGAGGTACC
                    ************************************************************

HsCD00434184        TGGTTTGCTGGCTTTGGCAGAGAACGCAACTCAGGCACCAAACTATTCAACATCTCTGGC
NM_007103.3         TGGTTTGCTGGCTTTGGCAGAGAACGCAACTCAGGCACCAAACTATTCAACATCTCTGGC
NM_001166102.1      TGGTTTGCTGGCTTTGGCAGAGAACGCAACTCAGGCACCAAACTATTCAACATCTCTGGC
                    ************************************************************

HsCD00434184        CATGTCAACCACCCTTGCACTGTGGAGGAGGAGATGTCTGTGCCCTTGAAAGAACTGATT
NM_007103.3         CATGTCAACCACCCTTGCACTGTGGAGGAGGAGATGTCTGTGCCCTTGAAAGAACTGATT
NM_001166102.1      CATGTCAACCACCCTTGCACTGTGGAGGAGGAGATGTCTGTGCCCTTGAAAGAACTGATT
                    ************************************************************

HsCD00434184        GAGAAGCATGCTGGGGGTGTCACGGGCGGCTGGGACAACCTCCTTGCTGTGATCCCTGGC
NM_007103.3         GAGAAGCATGCTGGGGGTGTCACGGGCGGCTGGGACAACCTCCTTGCTGTGATCCCTGGC
NM_001166102.1      GAGAAGCATGCTGGGGGTGTCACGGGCGGCTGGGACAACCTCCTTGCTGTGATCCCTGGC
                    ************************************************************

HsCD00434184        GGCTCGTCTACCCCACTGATCCCCAAGTCTGTGTGTGAGACGGTGCTGATGGACTTCGAT
NM_007103.3         GGCTCGTCTACCCCACTGATCCCCAAGTCTGTGTGTGAGACGGTGCTGATGGACTTCGAT
NM_001166102.1      GGCTCGTCTACCCCACTGATCCCCAAGTCTGTGTGTGAGACGGTGCTGATGGACTTCGAT
                    ************************************************************

HsCD00434184        GCGCTGGTGCAGGCACAGACAGGCCTGGGCACAGCTGCGGTGATCGTCATGGACCGCTCG
NM_007103.3         GCGCTGGTGCAGGCACAGACAGGCCTGGGCACAGCTGCGGTGATCGTCATGGACCGCTCG
NM_001166102.1      GCGCTGGTGCAGGCACAGACAGGCCTGGGCACAGCTGCGGTGATCGTCATGGACCGCTCG
                    ************************************************************

HsCD00434184        ACGGACATCGTGAAAGCCATCGCCCGCCTCATTGAGTTCTATAAGCACGAGAGCTGTGGC
NM_007103.3         ACGGACATCGTGAAAGCCATCGCCCGCCTCATTGAGTTCTATAAGCACGAGAGCTGTGGC
NM_001166102.1      ACGGACATCGTGAAAGCCATCGCCCGCCTCATTGAGTTCTATAAGCACGAGAGCTGTGGC
                    ************************************************************

HsCD00434184        CAGTGTACCCCATGCCGTGAGGGTGTGGACTGGATGAACAAGGTGATGGCACGTTTCGTG
NM_007103.3         CAGTGTACCCCATGCCGTGAGGGTGTGGACTGGATGAACAAGGTGATGGCACGTTTCGTG
NM_001166102.1      CAGTGTACCCCATGCCGTGAGGGTGTGGACTGGATGAACAAGGTGATGGCACGTTTCGTG
                    ************************************************************

HsCD00434184        AGGGGGGATGCCCGGCCGGCCGAGATCGACTCCCTGTGGGAGATCAGCAAGCAGATAGAA
NM_007103.3         AGGGGGGATGCCCGGCCGGCCGAGATCGACTCCCTGTGGGAGATCAGCAAGCAGATAGAA
NM_001166102.1      AGGGGGGATGCCCGGCCGGCCGAGATCGACTCCCTGTGGGAGATCAGCAAGCAGATAGAA
                    ************************************************************

HsCD00434184        GGCCATACGATTTGTGCTCTGGGTGACGGGGCCGCCTGGCCTGTGCAGGGTCTGATCCGC
NM_007103.3         GGCCATACGATTTGTGCTCTGGGTGACGGGGCCGCCTGGCCTGTGCAGGGTCTGATCCGC
NM_001166102.1      GGCCATACGATTTGTGCTCTGGGTGACGGGGCCGCCTGGCCTGTGCAGGGTCTGATCCGC
                    ************************************************************

HsCD00434184        CACTTTCGGCCGGAGCTCGAGGAGCGGATGCAGCGGTTTGCCCAGCAGCATCAGGCCCGG
NM_007103.3         CACTTTCGGCCGGAGCTCGAGGAGCGGATGCAGCGGTTTGCCCAGCAGCATCAGGCCCGG
NM_001166102.1      CACTTTCGGCCGGAGCTCGAGGAGCGGATGCAGCGGTTTGCCCAGCAGCATCAGGCCCGG
                    ************************************************************

HsCD00434184        CAGGCTGCCTCT---
NM_007103.3         CAGGCTGCCTCTTAG
NM_001166102.1      CAGGCTGCCTCTTAG
                    ************   

Pathways

Reactome : Metabolism
Reactome : Respiratory electron transport
Reactome : Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.
Reactome : The citric acid (TCA) cycle and respiratory electron transport
WikiPathway : Electron Transport Chain
WikiPathway : Oxidative phosphorylation

Clone

Original Clone ID : ccsbBroad304_01075

Organism


TAX_ID : 9606
Species Specific ID: 4723

Genome

Chromosome : 11
Map Location : 11q13
Ensembl : ENSG00000167792

Reagents

Labome : NDUFV1-antibody