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GNB2L1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GNB2L1
Gene Name:  guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH176787.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00043439

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 954nts         Open reading frame : 1 to 954
Reference Sequence Alignment

ATGACTGAGCAGATGACCCTTCGTGGCACCCTCAAGGGCCACAACGGCTGGGTAACCCAG
ATCGCTACTACCCCGCAGTTCCCGGACATGATCCTCTCCGCCTCTCGAGATAAGACCATC
ATCATGTGGAAACTGACCAGGGATGAGACCAACTATGGAATTCCACAGCGTGCTCTGCGG
GGTCACTCCCACTTTGTTAGTGATGTGGTTATCTCCTCAGATGGCCAGTTTGCCCTCTCA
GGCTCCTGGGATGGAACCCTGCGCCTCTGGGATCTCACAACGGGCACCACCACGAGGCGA
TTTGTGGGCCATACCAAGGATGTGCTGAGTGTGGCCTTCTCCTCTGACAACCGGCAGATT
GTCTCTGGATCTCGAGATAAAACCATCAAGCTATGGAATACCCTGGGTGTGTGCAAATAC
ACTGTCCAGGATGAGAGCCACTCAGAGTGGGTGTCTTGTGTCCGCTTCTCGCCCAACAGC
AGCAACCCTATCATCGTCTCCTGTGGCTGGGACAAGCTGGTCAAGGTATGGAACCTGGCT
AACTGCAAGCTGAAGACCAACCACATTGGCCACACAGGCTATCTGAACACGGTGACTGTC
TCTCCAGATGGATCCCTCTGTGCTTCTGGAGGCAAGGATGGCCAGGCCATGTTATGGGAT
CTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACATCATCAACGCCCTGTGC
TTCAGCCCTAACCGCTACTGGCTGTGTGCTGCCACAGGCCCCAGCATCAAGATCTGGGAT
TTAGAGGGAAAGATCATTGTAGATGAACTGAAGCAAGAAGTTATCAGTACCAGCAGCAAG
GCAGAACCACCCCAGTGCACCTCCCTGGCCTGGTCTGCTGATGGCCAGACTCTGTTTGCT
GGCTACACGGACAACCTGGTGCGAGTGTGGCAGGTGACCATTGGCACACGCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1057
Start on reference sequence 104
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GNB2L1
Symbol Nomenclature : GNB2L1
SYNONYM : Gnb2-rs1; H12.3; HLC-7; PIG21; RACK1
Designation : cell proliferation-inducing gene 21 protein|guanine nucleotide binding protein beta polypeptide 2-like 1|guanine nucleotide-binding protein subunit beta-2-like 1|guanine nucleotide-binding protein subunit beta-like protein 12.3|human lung cancer oncogene 7 protein|lung cancer oncogene 7|proliferation-inducing gene 21|protein homologous to chicken B complex protein, guanine nucleotide binding|receptor for activated C kinase 1|receptor of activated protein kinase C 1
Full Nomenclature : guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
GENEID : 10399
Locus Tag : HLC7
HGNC : 4399
MIM : 176981
Vega : OTTHUMG00000163380
Target GenBank: BC000366

 

Reference Sequence Alignment

  

HsCD00043439      ATGACTGAGCAGATGACCCTTCGTGGCACCCTCAAGGGCCACAACGGCTGGGTAACCCAG
NM_006098.4       ATGACTGAGCAGATGACCCTTCGTGGCACCCTCAAGGGCCACAACGGCTGGGTAACCCAG
                  ************************************************************

HsCD00043439      ATCGCTACTACCCCGCAGTTCCCGGACATGATCCTCTCCGCCTCTCGAGATAAGACCATC
NM_006098.4       ATCGCTACTACCCCGCAGTTCCCGGACATGATCCTCTCCGCCTCTCGAGATAAGACCATC
                  ************************************************************

HsCD00043439      ATCATGTGGAAACTGACCAGGGATGAGACCAACTATGGAATTCCACAGCGTGCTCTGCGG
NM_006098.4       ATCATGTGGAAACTGACCAGGGATGAGACCAACTATGGAATTCCACAGCGTGCTCTGCGG
                  ************************************************************

HsCD00043439      GGTCACTCCCACTTTGTTAGTGATGTGGTTATCTCCTCAGATGGCCAGTTTGCCCTCTCA
NM_006098.4       GGTCACTCCCACTTTGTTAGTGATGTGGTTATCTCCTCAGATGGCCAGTTTGCCCTCTCA
                  ************************************************************

HsCD00043439      GGCTCCTGGGATGGAACCCTGCGCCTCTGGGATCTCACAACGGGCACCACCACGAGGCGA
NM_006098.4       GGCTCCTGGGATGGAACCCTGCGCCTCTGGGATCTCACAACGGGCACCACCACGAGGCGA
                  ************************************************************

HsCD00043439      TTTGTGGGCCATACCAAGGATGTGCTGAGTGTGGCCTTCTCCTCTGACAACCGGCAGATT
NM_006098.4       TTTGTGGGCCATACCAAGGATGTGCTGAGTGTGGCCTTCTCCTCTGACAACCGGCAGATT
                  ************************************************************

HsCD00043439      GTCTCTGGATCTCGAGATAAAACCATCAAGCTATGGAATACCCTGGGTGTGTGCAAATAC
NM_006098.4       GTCTCTGGATCTCGAGATAAAACCATCAAGCTATGGAATACCCTGGGTGTGTGCAAATAC
                  ************************************************************

HsCD00043439      ACTGTCCAGGATGAGAGCCACTCAGAGTGGGTGTCTTGTGTCCGCTTCTCGCCCAACAGC
NM_006098.4       ACTGTCCAGGATGAGAGCCACTCAGAGTGGGTGTCTTGTGTCCGCTTCTCGCCCAACAGC
                  ************************************************************

HsCD00043439      AGCAACCCTATCATCGTCTCCTGTGGCTGGGACAAGCTGGTCAAGGTATGGAACCTGGCT
NM_006098.4       AGCAACCCTATCATCGTCTCCTGTGGCTGGGACAAGCTGGTCAAGGTATGGAACCTGGCT
                  ************************************************************

HsCD00043439      AACTGCAAGCTGAAGACCAACCACATTGGCCACACAGGCTATCTGAACACGGTGACTGTC
NM_006098.4       AACTGCAAGCTGAAGACCAACCACATTGGCCACACAGGCTATCTGAACACGGTGACTGTC
                  ************************************************************

HsCD00043439      TCTCCAGATGGATCCCTCTGTGCTTCTGGAGGCAAGGATGGCCAGGCCATGTTATGGGAT
NM_006098.4       TCTCCAGATGGATCCCTCTGTGCTTCTGGAGGCAAGGATGGCCAGGCCATGTTATGGGAT
                  ************************************************************

HsCD00043439      CTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACATCATCAACGCCCTGTGC
NM_006098.4       CTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACATCATCAACGCCCTGTGC
                  ************************************************************

HsCD00043439      TTCAGCCCTAACCGCTACTGGCTGTGTGCTGCCACAGGCCCCAGCATCAAGATCTGGGAT
NM_006098.4       TTCAGCCCTAACCGCTACTGGCTGTGTGCTGCCACAGGCCCCAGCATCAAGATCTGGGAT
                  ************************************************************

HsCD00043439      TTAGAGGGAAAGATCATTGTAGATGAACTGAAGCAAGAAGTTATCAGTACCAGCAGCAAG
NM_006098.4       TTAGAGGGAAAGATCATTGTAGATGAACTGAAGCAAGAAGTTATCAGTACCAGCAGCAAG
                  ************************************************************

HsCD00043439      GCAGAACCACCCCAGTGCACCTCCCTGGCCTGGTCTGCTGATGGCCAGACTCTGTTTGCT
NM_006098.4       GCAGAACCACCCCAGTGCACCTCCCTGGCCTGGTCTGCTGATGGCCAGACTCTGTTTGCT
                  ************************************************************

HsCD00043439      GGCTACACGGACAACCTGGTGCGAGTGTGGCAGGTGACCATTGGCACACGCTAG
NM_006098.4       GGCTACACGGACAACCTGGTGCGAGTGTGGCAGGTGACCATTGGCACACGCTAG
                  ******************************************************

Pathways

NCI : CXCR4-mediated signaling events
NCI : Hypoxic and oxygen homeostasis regulation of HIF-1-alpha
NCI : IGF1 pathway
NCI : Regulation of Androgen receptor activity
NCI : Syndecan-2-mediated signaling events
NCI : TNF receptor signaling pathway
NCI : Validated transcriptional targets of deltaNp63 isoforms
NCI : p73 transcription factor network
WikiPathway : Androgen receptor signaling pathway

Protein

SMART domain : WD40 : WD40 repeats
UniProt : P63244
UniProt : E9KL35
HPRD : 01503

Clone

Cloning Information : 176787
HIP Master Clone ID : 151932
Original Clone ID : FLH176787.01X

Organism


TAX_ID : 9606
Species Specific ID: 10399

Genome

Chromosome : 5
Map Location : 5q35.3
Ensembl : ENSG00000204628

Reagents

Labome : RACK1-antibody