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DUSP7 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  DUSP7
Gene Name:  dual specificity phosphatase 7
Sequence             
Original Clone ID: ccsbBroad304_10789
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00434660

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 961nts         Open reading frame : 1 to 961
Reference Sequence Alignment

ATGTTGCGCCGCCTGCGCAAGGGCAACCTGCCCATCCGCTCCATCATCCCCAACCACGCC
GACAAGGAGCGCTTCGCCACGCGCTGCAAGGCGGCCACCGTGCTGCTCTACGACGAGGCC
ACGGCCGAGTGGCAGCCCGAGCCCGGCGCTCCCGCCTCCGTGCTCGGCCTGCTCCTACAG
AAGCTGCGCGACGACGGCTGCCAGGCCTACTACCTCCAAGGTGGTTTCAACAAGTTTCAA
ACAGAGTACTCTGAGCACTGCGAGACCAACGTGGACAGCTCTTCCTCGCCGAGCAGCTCG
CCACCCACCTCAGTGCTGGGCCTGGGGGGCCTGCGCATCAGCTCTGACTGCTCCGACGGC
GAGTCGGACCGAGAGCTGCCCAGCAGTGCCACCGAGTCAGACGGCAGCCCTGTGCCATCC
AGCCAACCAGCCTTCCCTGTCCAGATCCTGCCCTACCTCTACCTCGGCTGCGCCAAGGAC
TCCACCAACCTGGACGTGCTCGGCAAGTATGGCATCAAGTATATCCTCAATGTCACACCC
AACCTACCCAACGCCTTCGAGCACGGCGGCGAGTTCACCTACAAGCAGATCCCCATCTCT
GACCACTGGAGCCAGAACCTCTCCCAGTTCTTCCCTGAGGCCATCAGCTTCATTGACGAA
GCCCGCTCCAAGAAGTGTGGTGTCCTGGTGCACTGCCTGGCAGGCATCAGCCGCTCAGTG
ACGGTCACTGTGGCCTATCTGATGCAGAAGATGAACCTGTCACTCAACGACGCCTACGAC
TTTGTCAAGAGGAAAAAGTCCAACATCTCGCCCAACTTCAACTTCATGGGGCAGCTGCTG
GACTTTGAGCGGACGCTGGGGCTAAGCAGCCCGTGCGACAACCACGCGTCGAGTGAGCAG
CTCTACTTTTCCACGCCCACCAACCACAACCTGTTCCCACTCAATACGCTGGAGTCCACG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : DUSP7
Symbol Nomenclature : DUSP7
SYNONYM : MKPX; PYST2
Designation : dual specificity protein phosphatase 7|dual specificity protein phosphatase PYST2|dual-specificity phosphatase-7
Full Nomenclature : dual specificity phosphatase 7
GENEID : 1849
HGNC : 3073
MIM : 602749
Vega : OTTHUMG00000157819
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00434660      ------------------------------------------------------------
NM_001947.3       ATGAAAAACCAGCTCCGCGGCCCCCCAGCGCGGGCGCACATGTCGACTTCGGGGGCGGCG
                                                                              

HsCD00434660      ------------------------------------------------------------
NM_001947.3       GCGGCTGGGGGCACCCGGGCGGGGTCCGAGCCCGGTGCGGGGTCGGGGTCCGGCGCAGGC
                                                                              

HsCD00434660      ------------------------------------------------------------
NM_001947.3       ACCGGGGCGGGCGCGGCGACGGGGGCAGGGGCCATGCCCTGCAAGAGCGCCGAGTGGCTG
                                                                              

HsCD00434660      ------------------------------------------------------------
NM_001947.3       CAGGAGGAGCTGGAGGCGCGCGGCGGCGCGTCCTTGCTGCTGCTCGACTGCCGGCCGCAC
                                                                              

HsCD00434660      ---------------------------------------------------------ATG
NM_001947.3       GAGCTCTTCGAGTCGTCGCACATCGAGACGGCCATCAACCTGGCCATCCCGGGCCTCATG
                                                                           ***

HsCD00434660      TTGCGCCGCCTGCGCAAGGGCAACCTGCCCATCCGCTCCATCATCCCCAACCACGCCGAC
NM_001947.3       TTGCGCCGCCTGCGCAAGGGCAACCTGCCCATCCGCTCCATCATCCCCAACCACGCCGAC
                  ************************************************************

HsCD00434660      AAGGAGCGCTTCGCCACGCGCTGCAAGGCGGCCACCGTGCTGCTCTACGACGAGGCCACG
NM_001947.3       AAGGAGCGCTTCGCCACGCGCTGCAAGGCGGCCACCGTGCTGCTCTACGACGAGGCCACG
                  ************************************************************

HsCD00434660      GCCGAGTGGCAGCCCGAGCCCGGCGCTCCCGCCTCCGTGCTCGGCCTGCTCCTACAGAAG
NM_001947.3       GCCGAGTGGCAGCCCGAGCCCGGCGCTCCCGCCTCCGTGCTCGGCCTGCTCCTACAGAAG
                  ************************************************************

HsCD00434660      CTGCGCGACGACGGCTGCCAGGCCTACTACCTCCAAGGTGGTTTCAACAAGTTTCAAACA
NM_001947.3       CTGCGCGACGACGGCTGCCAGGCCTACTACCTCCAAGGTGGTTTCAACAAGTTTCAAACA
                  ************************************************************

HsCD00434660      GAGTACTCTGAGCACTGCGAGACCAACGTGGACAGCTCTTCCTCGCCGAGCAGCTCGCCA
NM_001947.3       GAGTACTCTGAGCACTGCGAGACCAACGTGGACAGCTCTTCCTCGCCGAGCAGCTCGCCA
                  ************************************************************

HsCD00434660      CCCACCTCAGTGCTGGGCCTGGGGGGCCTGCGCATCAGCTCTGACTGCTCCGACGGCGAG
NM_001947.3       CCCACCTCAGTGCTGGGCCTGGGGGGCCTGCGCATCAGCTCTGACTGCTCCGACGGCGAG
                  ************************************************************

HsCD00434660      TCGGACCGAGAGCTGCCCAGCAGTGCCACCGAGTCAGACGGCAGCCCTGTGCCATCCAGC
NM_001947.3       TCGGACCGAGAGCTGCCCAGCAGTGCCACCGAGTCAGACGGCAGCCCTGTGCCATCCAGC
                  ************************************************************

HsCD00434660      CAACCAGCCTTCCCTGTCCAGATCCTGCCCTACCTCTACCTCGGCTGCGCCAAGGACTCC
NM_001947.3       CAACCAGCCTTCCCTGTCCAGATCCTGCCCTACCTCTACCTCGGCTGCGCCAAGGACTCC
                  ************************************************************

HsCD00434660      ACCAACCTGGACGTGCTCGGCAAGTATGGCATCAAGTATATCCTCAATGTCACACCCAAC
NM_001947.3       ACCAACCTGGACGTGCTCGGCAAGTATGGCATCAAGTATATCCTCAATGTCACACCCAAC
                  ************************************************************

HsCD00434660      CTACCCAACGCCTTCGAGCACGGCGGCGAGTTCACCTACAAGCAGATCCCCATCTCTGAC
NM_001947.3       CTACCCAACGCCTTCGAGCACGGCGGCGAGTTCACCTACAAGCAGATCCCCATCTCTGAC
                  ************************************************************

HsCD00434660      CACTGGAGCCAGAACCTCTCCCAGTTCTTCCCTGAGGCCATCAGCTTCATTGACGAAGCC
NM_001947.3       CACTGGAGCCAGAACCTCTCCCAGTTCTTCCCTGAGGCCATCAGCTTCATTGACGAAGCC
                  ************************************************************

HsCD00434660      CGCTCCAAGAAGTGTGGTGTCCTGGTGCACTGCCTGGCAGGCATCAGCCGCTCAGTGACG
NM_001947.3       CGCTCCAAGAAGTGTGGTGTCCTGGTGCACTGCCTGGCAGGCATCAGCCGCTCAGTGACG
                  ************************************************************

HsCD00434660      GTCACTGTGGCCTATCTGATGCAGAAGATGAACCTGTCACTCAACGACGCCTACGACTTT
NM_001947.3       GTCACTGTGGCCTATCTGATGCAGAAGATGAACCTGTCACTCAACGACGCCTACGACTTT
                  ************************************************************

HsCD00434660      GTCAAGAGGAAAAAGTCCAACATCTCGCCCAACTTCAACTTCATGGGGCAGCTGCTGGAC
NM_001947.3       GTCAAGAGGAAAAAGTCCAACATCTCGCCCAACTTCAACTTCATGGGGCAGCTGCTGGAC
                  ************************************************************

HsCD00434660      TTTGAGCGGACGCTGGGGCTAAGCAGCCCGTGCGACAACCACGCGTCGAGTGAGCAGCTC
NM_001947.3       TTTGAGCGGACGCTGGGGCTAAGCAGCCCGTGCGACAACCACGCGTCGAGTGAGCAGCTC
                  ************************************************************

HsCD00434660      TACTTTTCCACGCCCACCAACCACAACCTGTTCCCACTCAATACGCTGGAGTCCACG---
NM_001947.3       TACTTTTCCACGCCCACCAACCACAACCTGTTCCCACTCAATACGCTGGAGTCCACGTGA
                  *********************************************************   

Pathways

Reactome : Activated TLR4 signalling
Reactome : ERK/MAPK targets
Reactome : ERKs are inactivated
Reactome : Immune System
Reactome : Innate Immune System
Reactome : MAP kinase activation in TLR cascade
Reactome : MAPK targets/ Nuclear events mediated by MAP kinases
Reactome : MyD88 cascade initiated on plasma membrane
Reactome : MyD88 dependent cascade initiated on endosome
Reactome : MyD88-independent cascade
Reactome : MyD88:Mal cascade initiated on plasma membrane
Reactome : NGF signalling via TRKA from the plasma membrane
Reactome : Nuclear Events (kinase and transcription factor activation)
Reactome : Signal Transduction
Reactome : Signalling by NGF
Reactome : TRAF6 Mediated Induction of proinflammatory cytokines
Reactome : TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
Reactome : TRIF-mediated TLR3/TLR4 signaling
Reactome : Toll Like Receptor 10 (TLR10) Cascade
Reactome : Toll Like Receptor 2 (TLR2) Cascade
Reactome : Toll Like Receptor 3 (TLR3) Cascade
Reactome : Toll Like Receptor 4 (TLR4) Cascade
Reactome : Toll Like Receptor 5 (TLR5) Cascade
Reactome : Toll Like Receptor 7/8 (TLR7/8) Cascade
Reactome : Toll Like Receptor 9 (TLR9) Cascade
Reactome : Toll Like Receptor TLR1:TLR2 Cascade
Reactome : Toll Like Receptor TLR6:TLR2 Cascade
Reactome : Toll-Like Receptors Cascades
WikiPathway : MAPK signaling pathway

Clone

Original Clone ID : ccsbBroad304_10789

Organism


TAX_ID : 9606
Species Specific ID: 1849

Genome

Chromosome : 3
Map Location : 3p21
Ensembl : ENSG00000164086

Reagents

Labome : DUSP7-antibody