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P2RY14 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  P2RY14
Gene Name:  purinergic receptor P2Y, G-protein coupled, 14
Sequence             
Original Clone ID: ccsbBroad304_07513
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00434780

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1015nts         Open reading frame : 1 to 1015
Reference Sequence Alignment

ATGATCAATTCAACCTCCACACAGCCTCCAGATGAATCCTGCTCTCAGAACCTCCTGATC
ACTCAGCAGATCATTCCTGTGCTGTACTGTATGGTCTTCATTGCAGGAATCCTACTCAAT
GGAGTGTCAGGATGGATATTCTTTTACGTGCCCAGCTCTGAGAGTTTCATCATCTATCTC
AAGAACATTGTTATTGCTGACTTTGTGATGAGCCTGACTTTTCCTTTCAAGATCCTTGGT
GACTCAGGCCTTGGTCCCTGGCAGCTGAACGTGTTTGTGTGCAGGGTCTCTGCCGTGCTC
TTCTACGTCAACATGTACGTCAGCATTGTGTTCTTTGGGCTCATCAGCTTTGACAGATAT
TATAAAATTGTAAAGCCTCTTTGGACTTCTTTCATCCAGTCAGTGAGTTACAGCAAACTT
CTGTCAGTGATAGTATGGATGCTCATGCTCCTCCTTGCTGTTCCAAATATTATTCTCACC
AACCAGAGTGTTAGGGAGGTTACACAAATAAAATGTATAGAACTGAAAAGTGAACTGGGA
CGGAAGTGGCACAAAGCATCAAACTACATCTTCGTGGCCATCTTCTGGATTGTGTTTCTT
TTGTTAATCGTTTTCTATACTGCTATCACAAAGAAAATCTTTAAGTCCCACCTTAAGTCA
AGTCGGAATTCCACTTCGGTCAAAAAGAAATCTAGCCGCAACATATTCAGCATCGTGTTT
GTGTTTTTTGTCTGTTTTGTACCTTACCATATTGCCAGAATCCCCTACACAAAGAGTCAG
ACCGAAGCTCATTACAGCTGCCAGTCAAAAGAAATCTTGCGGTATATGAAAGAATTCACT
CTGCTACTATCTGCTGCAAATGTATGCTTGGACCCTATTATTTATTTCTTTCTATGCCAG
CCGTTTAGGGAAATCTTATGTAAGAAATTGCACATTCCATTAAAAGCTCAGAATGACCTA
GACATTTCCAGAATCAAAAGAGGAAATACAACACTTGAAAGCACAGATACTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : P2RY14
Symbol Nomenclature : P2RY14
SYNONYM : BPR105; GPR105; P2Y14
Designation : G protein coupled receptor for UDP-glucose|G protein-coupled receptor 105|G-protein coupled receptor 105|P2Y purinoceptor 14|P2Y(14) receptor|P2Y14 receptor|UDP-glucose receptor
Full Nomenclature : purinergic receptor P2Y, G-protein coupled, 14
GENEID : 9934
HGNC : 16442
MIM : 610116
Vega : OTTHUMG00000159859
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00434780        ATGATCAATTCAACCTCCACACAGCCTCCAGATGAATCCTGCTCTCAGAACCTCCTGATC
NM_014879.3         ATGATCAATTCAACCTCCACACAGCCTCCAGATGAATCCTGCTCTCAGAACCTCCTGATC
XM_005247921.1      ATGATCAATTCAACCTCCACACAGCCTCCAGATGAATCCTGCTCTCAGAACCTCCTGATC
NM_001081455.1      ATGATCAATTCAACCTCCACACAGCCTCCAGATGAATCCTGCTCTCAGAACCTCCTGATC
XM_005247923.1      ATGATCAATTCAACCTCCACACAGCCTCCAGATGAATCCTGCTCTCAGAACCTCCTGATC
                    ************************************************************

HsCD00434780        ACTCAGCAGATCATTCCTGTGCTGTACTGTATGGTCTTCATTGCAGGAATCCTACTCAAT
NM_014879.3         ACTCAGCAGATCATTCCTGTGCTGTACTGTATGGTCTTCATTGCAGGAATCCTACTCAAT
XM_005247921.1      ACTCAGCAGATCATTCCTGTGCTGTACTGTATGGTCTTCATTGCAGGAATCCTACTCAAT
NM_001081455.1      ACTCAGCAGATCATTCCTGTGCTGTACTGTATGGTCTTCATTGCAGGAATCCTACTCAAT
XM_005247923.1      ACTCAGCAGATCATTCCTGTGCTGTACTGTATGGTCTTCATTGCAGGAATCCTACTCAAT
                    ************************************************************

HsCD00434780        GGAGTGTCAGGATGGATATTCTTTTACGTGCCCAGCTCTGAGAGTTTCATCATCTATCTC
NM_014879.3         GGAGTGTCAGGATGGATATTCTTTTACGTGCCCAGCTCTAAGAGTTTCATCATCTATCTC
XM_005247921.1      GGAGTGTCAGGATGGATATTCTTTTACGTGCCCAGCTCTAAGAGTTTCATCATCTATCTC
NM_001081455.1      GGAGTGTCAGGATGGATATTCTTTTACGTGCCCAGCTCTAAGAGTTTCATCATCTATCTC
XM_005247923.1      GGAGTGTCAGGATGGATATTCTTTTACGTGCCCAGCTCTAAGAGTTTCATCATCTATCTC
                    ***************************************.********************

HsCD00434780        AAGAACATTGTTATTGCTGACTTTGTGATGAGCCTGACTTTTCCTTTCAAGATCCTTGGT
NM_014879.3         AAGAACATTGTTATTGCTGACTTTGTGATGAGCCTGACTTTTCCTTTCAAGATCCTTGGT
XM_005247921.1      AAGAACATTGTTATTGCTGACTTTGTGATGAGCCTGACTTTTCCTTTCAAGATCCTTGGT
NM_001081455.1      AAGAACATTGTTATTGCTGACTTTGTGATGAGCCTGACTTTTCCTTTCAAGATCCTTGGT
XM_005247923.1      AAGAACATTGTTATTGCTGACTTTGTGATGAGCCTGACTTTTCCTTTCAAGATCCTTGGT
                    ************************************************************

HsCD00434780        GACTCAGGCCTTGGTCCCTGGCAGCTGAACGTGTTTGTGTGCAGGGTCTCTGCCGTGCTC
NM_014879.3         GACTCAGGCCTTGGTCCCTGGCAGCTGAACGTGTTTGTGTGCAGGGTCTCTGCCGTGCTC
XM_005247921.1      GACTCAGGCCTTGGTCCCTGGCAGCTGAACGTGTTTGTGTGCAGGGTCTCTGCCGTGCTC
NM_001081455.1      GACTCAGGCCTTGGTCCCTGGCAGCTGAACGTGTTTGTGTGCAGGGTCTCTGCCGTGCTC
XM_005247923.1      GACTCAGGCCTTGGTCCCTGGCAGCTGAACGTGTTTGTGTGCAGGGTCTCTGCCGTGCTC
                    ************************************************************

HsCD00434780        TTCTACGTCAACATGTACGTCAGCATTGTGTTCTTTGGGCTCATCAGCTTTGACAGATAT
NM_014879.3         TTCTACGTCAACATGTACGTCAGCATTGTGTTCTTTGGGCTCATCAGCTTTGACAGATAT
XM_005247921.1      TTCTACGTCAACATGTACGTCAGCATTGTGTTCTTTGGGCTCATCAGCTTTGACAGATAT
NM_001081455.1      TTCTACGTCAACATGTACGTCAGCATTGTGTTCTTTGGGCTCATCAGCTTTGACAGATAT
XM_005247923.1      TTCTACGTCAACATGTACGTCAGCATTGTGTTCTTTGGGCTCATCAGCTTTGACAGATAT
                    ************************************************************

HsCD00434780        TATAAAATTGTAAAGCCTCTTTGGACTTCTTTCATCCAGTCAGTGAGTTACAGCAAACTT
NM_014879.3         TATAAAATTGTAAAGCCTCTTTGGACTTCTTTCATCCAGTCAGTGAGTTACAGCAAACTT
XM_005247921.1      TATAAAATTGTAAAGCCTCTTTGGACTTCTTTCATCCAGTCAGTGAGTTACAGCAAACTT
NM_001081455.1      TATAAAATTGTAAAGCCTCTTTGGACTTCTTTCATCCAGTCAGTGAGTTACAGCAAACTT
XM_005247923.1      TATAAAATTGTAAAGCCTCTTTGGACTTCTTTCATCCAGTCAGTGAGTTACAGCAAACTT
                    ************************************************************

HsCD00434780        CTGTCAGTGATAGTATGGATGCTCATGCTCCTCCTTGCTGTTCCAAATATTATTCTCACC
NM_014879.3         CTGTCAGTGATAGTATGGATGCTCATGCTCCTCCTTGCTGTTCCAAATATTATTCTCACC
XM_005247921.1      CTGTCAGTGATAGTATGGATGCTCATGCTCCTCCTTGCTGTTCCAAATATTATTCTCACC
NM_001081455.1      CTGTCAGTGATAGTATGGATGCTCATGCTCCTCCTTGCTGTTCCAAATATTATTCTCACC
XM_005247923.1      CTGTCAGTGATAGTATGGATGCTCATGCTCCTCCTTGCTGTTCCAAATATTATTCTCACC
                    ************************************************************

HsCD00434780        AACCAGAGTGTTAGGGAGGTTACACAAATAAAATGTATAGAACTGAAAAGTGAACTGGGA
NM_014879.3         AACCAGAGTGTTAGGGAGGTTACACAAATAAAATGTATAGAACTGAAAAGTGAACTGGGA
XM_005247921.1      AACCAGAGTGTTAGGGAGGTTACACAAATAAAATGTATAGAACTGAAAAGTGAACTGGGA
NM_001081455.1      AACCAGAGTGTTAGGGAGGTTACACAAATAAAATGTATAGAACTGAAAAGTGAACTGGGA
XM_005247923.1      AACCAGAGTGTTAGGGAGGTTACACAAATAAAATGTATAGAACTGAAAAGTGAACTGGGA
                    ************************************************************

HsCD00434780        CGGAAGTGGCACAAAGCATCAAACTACATCTTCGTGGCCATCTTCTGGATTGTGTTTCTT
NM_014879.3         CGGAAGTGGCACAAAGCATCAAACTACATCTTCGTGGCCATCTTCTGGATTGTGTTTCTT
XM_005247921.1      CGGAAGTGGCACAAAGCATCAAACTACATCTTCGTGGCCATCTTCTGGATTGTGTTTCTT
NM_001081455.1      CGGAAGTGGCACAAAGCATCAAACTACATCTTCGTGGCCATCTTCTGGATTGTGTTTCTT
XM_005247923.1      CGGAAGTGGCACAAAGCATCAAACTACATCTTCGTGGCCATCTTCTGGATTGTGTTTCTT
                    ************************************************************

HsCD00434780        TTGTTAATCGTTTTCTATACTGCTATCACAAAGAAAATCTTTAAGTCCCACCTTAAGTCA
NM_014879.3         TTGTTAATCGTTTTCTATACTGCTATCACAAAGAAAATCTTTAAGTCCCACCTTAAGTCA
XM_005247921.1      TTGTTAATCGTTTTCTATACTGCTATCACAAAGAAAATCTTTAAGTCCCACCTTAAGTCA
NM_001081455.1      TTGTTAATCGTTTTCTATACTGCTATCACAAAGAAAATCTTTAAGTCCCACCTTAAGTCA
XM_005247923.1      TTGTTAATCGTTTTCTATACTGCTATCACAAAGAAAATCTTTAAGTCCCACCTTAAGTCA
                    ************************************************************

HsCD00434780        AGTCGGAATTCCACTTCGGTCAAAAAGAAATCTAGCCGCAACATATTCAGCATCGTGTTT
NM_014879.3         AGTCGGAATTCCACTTCGGTCAAAAAGAAATCTAGCCGCAACATATTCAGCATCGTGTTT
XM_005247921.1      AGTCGGAATTCCACTTCGGTCAAAAAGAAATCTAGCCGCAACATATTCAGCATCGTGTTT
NM_001081455.1      AGTCGGAATTCCACTTCGGTCAAAAAGAAATCTAGCCGCAACATATTCAGCATCGTGTTT
XM_005247923.1      AGTCGGAATTCCACTTCGGTCAAAAAGAAATCTAGCCGCAACATATTCAGCATCGTGTTT
                    ************************************************************

HsCD00434780        GTGTTTTTTGTCTGTTTTGTACCTTACCATATTGCCAGAATCCCCTACACAAAGAGTCAG
NM_014879.3         GTGTTTTTTGTCTGTTTTGTACCTTACCATATTGCCAGAATCCCCTACACAAAGAGTCAG
XM_005247921.1      GTGTTTTTTGTCTGTTTTGTACCTTACCATATTGCCAGAATCCCCTACACAAAGAGTCAG
NM_001081455.1      GTGTTTTTTGTCTGTTTTGTACCTTACCATATTGCCAGAATCCCCTACACAAAGAGTCAG
XM_005247923.1      GTGTTTTTTGTCTGTTTTGTACCTTACCATATTGCCAGAATCCCCTACACAAAGAGTCAG
                    ************************************************************

HsCD00434780        ACCGAAGCTCATTACAGCTGCCAGTCAAAAGAAATCTTGCGGTATATGAAAGAATTCACT
NM_014879.3         ACCGAAGCTCATTACAGCTGCCAGTCAAAAGAAATCTTGCGGTATATGAAAGAATTCACT
XM_005247921.1      ACCGAAGCTCATTACAGCTGCCAGTCAAAAGAAATCTTGCGGTATATGAAAGAATTCACT
NM_001081455.1      ACCGAAGCTCATTACAGCTGCCAGTCAAAAGAAATCTTGCGGTATATGAAAGAATTCACT
XM_005247923.1      ACCGAAGCTCATTACAGCTGCCAGTCAAAAGAAATCTTGCGGTATATGAAAGAATTCACT
                    ************************************************************

HsCD00434780        CTGCTACTATCTGCTGCAAATGTATGCTTGGACCCTATTATTTATTTCTTTCTATGCCAG
NM_014879.3         CTGCTACTATCTGCTGCAAATGTATGCTTGGACCCTATTATTTATTTCTTTCTATGCCAG
XM_005247921.1      CTGCTACTATCTGCTGCAAATGTATGCTTGGACCCTATTATTTATTTCTTTCTATGCCAG
NM_001081455.1      CTGCTACTATCTGCTGCAAATGTATGCTTGGACCCTATTATTTATTTCTTTCTATGCCAG
XM_005247923.1      CTGCTACTATCTGCTGCAAATGTATGCTTGGACCCTATTATTTATTTCTTTCTATGCCAG
                    ************************************************************

HsCD00434780        CCGTTTAGGGAAATCTTATGTAAGAAATTGCACATTCCATTAAAAGCTCAGAATGACCTA
NM_014879.3         CCGTTTAGGGAAATCTTATGTAAGAAATTGCACATTCCATTAAAAGCTCAGAATGACCTA
XM_005247921.1      CCGTTTAGGGAAATCTTATGTAAGAAATTGCACATTCCATTAAAAGCTCAGAATGACCTA
NM_001081455.1      CCGTTTAGGGAAATCTTATGTAAGAAATTGCACATTCCATTAAAAGCTCAGAATGACCTA
XM_005247923.1      CCGTTTAGGGAAATCTTATGTAAGAAATTGCACATTCCATTAAAAGCTCAGAATGACCTA
                    ************************************************************

HsCD00434780        GACATTTCCAGAATCAAAAGAGGAAATACAACACTTGAAAGCACAGATACTTTG---
NM_014879.3         GACATTTCCAGAATCAAAAGAGGAAATACAACACTTGAAAGCACAGATACTTTGTGA
XM_005247921.1      GACATTTCCAGAATCAAAAGAGGAAATACAACACTTGAAAGCACAGATACTTTGTGA
NM_001081455.1      GACATTTCCAGAATCAAAAGAGGAAATACAACACTTGAAAGCACAGATACTTTGTGA
XM_005247923.1      GACATTTCCAGAATCAAAAGAGGAAATACAACACTTGAAAGCACAGATACTTTGTGA
                    ******************************************************   

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (i) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Nucleotide-like (purinergic) receptors
Reactome : P2Y receptors
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class A Rhodopsin-like

Protein

HPRD : 08820

Clone

Original Clone ID : ccsbBroad304_07513

Organism


TAX_ID : 9606
Species Specific ID: 9934

Genome

Chromosome : 3
Map Location : 3q24-q25.1
Ensembl : ENSG00000174944

Reagents

Labome : P2RY14-antibody