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PHLDA1 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PHLDA1
Gene Name:  pleckstrin homology-like domain, family A, member 1
Sequence             
Original Clone ID: ccsbBroad304_14068
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: PMID: 24954507
Title: PHLDA1 expression is controlled by an estrogen receptor-NFkappaB-miR-181 regulatory loop and is essential for formation of ER+ mammospheres
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00434827

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 778nts         Open reading frame : 1 to 778

ATGCTGGAGAGTAGCGGCNGCAAAGCGCTGAAGGAGGGCGTGCTGGAGAAGCGCAGCGAC
GGGTTGTTGCAGCTCTGGAAGAAAAAGTGTTGCATCCTCACCGAGGAAGGGCTGCTGCTT
ATCCCGCCCAAGCAGCTGCAACACCAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAA
CAACAGCCCGGGCAGGGGCCGGCCGAGCCGTCCCAACCCAGTGGCCCCGCTGTCGCCAGC
CTCGAGCCGCCGGTCAAGCTCAAGGAACTGCACTTCTCCAACATGAAGACCGTGGACTGT
GTGGAGCGCAAGGGCAAGTACATGTACTTCACTGTGGTGATGGCAGAGGGCAAGGAGATC
GACTTTCGGTGCCCGCAAGACCAGGGCTGGAACGCCGAGATCACGCTGCAGATGGTGCAG
TACAAGAATCGTCAGGCCATCCTGGCGGTCAAATCCACGCGGCAGAAGCAGCAGCACCTG
GTCCAGCAGCAGCCCCCCTCGCAGCCGCAGCCGCAGCCGCAGCTCCAGCCCCAACCCCAG
CCTCAGCCTCAGCCGCAACCCCAGCCCCAATCACAACCCCAGCCTCAGCCCCAACCCAAG
CCTCAGCCCCAGCAGCTCCACCCGTATCCGCATCCACATCCACATCCACACTCTCATCCT
CACTCGCACCCACACCCTCACCCGCACCCGCATCCGCACCAAATACCGCACCCACACCCA
CAGCCGCACTCGCAGCCGCACGGGCACCGGCTTCTCCGCAGCACCTCCAACTCTGCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
1 error; 1 non-synonomous errors;
 Clone mixture?
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PHLDA1
Symbol Nomenclature : PHLDA1
SYNONYM : DT1P1B11; PHRIP; TDAG51
Designation : PQ-rich protein|PQR protein|T-cell death-associated gene 51 protein|apoptosis-associated nuclear protein|pleckstrin homology-like domain family A member 1|proline- and glutamine-rich protein|proline- and histidine-rich protein|proline-histidine rich protein
Full Nomenclature : pleckstrin homology-like domain, family A, member 1
GENEID : 22822
HGNC : 8933
MIM : 605335
Target GenBank: None

 

Protein

SMART domain : PH : Pleckstrin homology domain.
HPRD : 05625

Clone

Original Clone ID : ccsbBroad304_14068

Organism


TAX_ID : 9606
Species Specific ID: 22822

Genome

Chromosome : 12
Map Location : 12q15

Reagents

Labome : PHLDA1-antibody