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SRPR (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  SRPR
Gene Name:  signal recognition particle receptor (docking protein)
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH177318.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00043493

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1917nts         Open reading frame : 1 to 1917
Reference Sequence Alignment

ATGCTCGACTTCTTCACCATTTTCTCCAAGGGCGGGCTTGTGCTCTGGTGCTTCCAGGGC
GTTAGCGACTCATGCACCGGACCCGTTAACGCGTTGATTCGTTCCGTGCTGCTGCAGGAA
CGGGGAGGTAACAACTCCTTCACCCATGAGGCACTCACACTCAAGTATAAACTGGACAAC
CAGTTTGAGCTGGTGTTTGTGGTTGGTTTTCAGAAGATCCTGACACTGACATATGTAGAC
AAATTGATAGATGACGTGCATCGGCTGTTTCGGGACAAGTACCGCACAGAGATCCAACAG
CAAAGTGCTTTAAGTTTATTAAATGGCACTTTTGATTTCCAAAATGACTTCCTGCGGCTC
CTTCGTGAAGCAGAGGAGAGCAGTAAGATCCGTGCTCCCACTACCATGAAGAAATTTGAA
GATTCTGAAAAGGCCAAGAAACCTGTGAGGTCCATGATTGAGACACGGGGGGAAAAGCCC
AAGGAAAAAGCAAAGAATAGCAAAAAAAAGGGGGCCAAGAAGGAAGGTTCTGATGGTCCT
TTGGCTACCAGCAAACCAGTCCCTGCAGAAAAGTCAGGTCTTCCAGTGGGTCCTGAGAAC
GGAGTAGAACTTTCCAAAGAGGAGCTGATCCGCAGGAAGCGCGAGGAGTTCATTCAGAAG
CATGGGAGGGGTATGGAGAAGTCCAACAAGTCCACGAAGTCAGATGCTCCAAAGGAGAAG
GGCAAAAAAGCACCCCGGGTGTGGGAACTGGGTGGCTGTGCTAACAAAGAAGTGTTGGAT
TACAGTACTCCCACCACCAATGGAACCCCTGAGGCTGCCTTGTCTGAGGACATCAACCTG
ATTCGAGGGACTGGGTCTGGGGGGCAGCTTCAGGATCTGGACTGCAGCAGCTCTGATGAC
GAAGGGGCTGCTCAAAACTCTACCAAACCTAGTGCGACCAAGGGAACACTGGGTGGCATG
TTTGGTATGCTGAAGGGCCTTGTGGGTTCAAAGAGCTTGAGTCGTGAAGACATGGAATCT
GTGCTGGACAAGATGCGTGATCATCTCATTGCTAAGAACGTGGCTGCAGACATTGCCGTC
CAGCTCTGTGAATCTGTTGCCAACAAGTTGGAAGGGAAGGTGATGGGGACGTTCAGCACG
GTGACTTCCACAGTAAAGCAAGCCCTACAGGAGTCCCTGGTGCAGATTCTGCAGCCACAG
CGTCGTGTAGACATGCTCCGGGACATCATGGATGCCCAGCGTCGCCAGCGCCCTTATGTC
GTCACCTTCTGCGGCGTTAATGGAGTGGGGAAATCTACTAATCTTGCCAAGATTTCCTTC
TGGTTGTTAGAGAATGGCTTCAGTGTCCTCATTGCTGCCTGTGATACATTTCGTGCTGGG
GCCGTGGAGCAGCTGCGTACACACACCCGGCGTTTGAGTGCCCTACACCCTCCAGAGAAG
CATGGTGGCCGCACCATGGTGCAGTTGTTTGAAAAGGGCTATGGCAAGGATGCTGCTGGC
ATTGCCATGGAAGCCATTGCTTTTGCACGTAACCAAGGCTTTGACGTGGTGCTGGTGGAC
ACGGCAGGCCGCATGCAAGACAATGCCCCTCTGATGACTGCCCTGGCCAAACTCATTACT
GTCAATACACCTGATTTGGTGCTGTTTGTAGGAGAAGCCTTAGTAGGCAATGAAGCCGTG
GACCAGCTGGTCAAGTTCAACAGAGCCTTGGCTGACCATTCTATGGCTCAGACACCTCGG
CTCATTGATGGCATTGTTCTTACCAAATTTGATACCATTGATGACAAGGTGGGAGCTGCT
ATTTCTATGACGTACATCACAAGCAAACCCATCGTCTTTGTGGGCACCGGCCAGACCTAC
TGTGACCTACGCAGCCTCAATGCCAAGGCTGTGGTGGCTGCCCTCATGAAGGCTTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2001
Start on reference sequence 85
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SRPR
Symbol Nomenclature : SRPR
SYNONYM : DP; Sralpha
Designation : DP-alpha|SR-alpha|SRP-alpha|docking protein alpha|signal recognition particle receptor subunit alpha
Full Nomenclature : signal recognition particle receptor (docking protein)
GENEID : 6734
HGNC : 11307
MIM : 182180
Vega : OTTHUMG00000165826
Target GenBank: BC013583

 

Reference Sequence Alignment

  

HsCD00043493        ATGCTCGACTTCTTCACCATTTTCTCCAAGGGCGGGCTTGTGCTCTGGTGCTTCCAGGGC
NM_003139.3         ATGCTCGACTTCTTCACCATTTTCTCCAAGGGCGGGCTTGTGCTCTGGTGCTTCCAGGGC
NM_001177842.1      ATGCTCGACTTCTTCACCATTTTCTCCAAGGGCGGGCTTGTGCTCTGGTGCTTCCAGGGC
                    ************************************************************

HsCD00043493        GTTAGCGACTCATGCACCGGACCCGTTAACGCGTTGATTCGTTCCGTGCTGCTGCAGGAA
NM_003139.3         GTTAGCGACTCATGCACCGGACCCGTTAACGCGTTGATTCGTTCCGTGCTGCTGCAGGAA
NM_001177842.1      GTTAGCGACTCATGCACCGGACCCGTTAACGCGTTGATTCGTTCCGTGCTGCTGCAG---
                    *********************************************************   

HsCD00043493        CGGGGAGGTAACAACTCCTTCACCCATGAGGCACTCACACTCAAGTATAAACTGGACAAC
NM_003139.3         CGGGGAGGTAACAACTCCTTCACCCATGAGGCACTCACACTCAAGTATAAACTGGACAAC
NM_001177842.1      ------------------------------------------------------------
                                                                                

HsCD00043493        CAGTTTGAGCTGGTGTTTGTGGTTGGTTTTCAGAAGATCCTGACACTGACATATGTAGAC
NM_003139.3         CAGTTTGAGCTGGTGTTTGTGGTTGGTTTTCAGAAGATCCTGACACTGACATATGTAGAC
NM_001177842.1      ---------------------GTTGGTTTTCAGAAGATCCTGACACTGACATATGTAGAC
                                         ***************************************

HsCD00043493        AAATTGATAGATGACGTGCATCGGCTGTTTCGGGACAAGTACCGCACAGAGATCCAACAG
NM_003139.3         AAATTGATAGATGACGTGCATCGGCTGTTTCGGGACAAGTACCGCACAGAGATCCAACAG
NM_001177842.1      AAATTGATAGATGACGTGCATCGGCTGTTTCGGGACAAGTACCGCACAGAGATCCAACAG
                    ************************************************************

HsCD00043493        CAAAGTGCTTTAAGTTTATTAAATGGCACTTTTGATTTCCAAAATGACTTCCTGCGGCTC
NM_003139.3         CAAAGTGCTTTAAGTTTATTAAATGGCACTTTTGATTTCCAAAATGACTTCCTGCGGCTC
NM_001177842.1      CAAAGTGCTTTAAGTTTATTAAATGGCACTTTTGATTTCCAAAATGACTTCCTGCGGCTC
                    ************************************************************

HsCD00043493        CTTCGTGAAGCAGAGGAGAGCAGTAAGATCCGTGCTCCCACTACCATGAAGAAATTTGAA
NM_003139.3         CTTCGTGAAGCAGAGGAGAGCAGTAAGATCCGTGCTCCCACTACCATGAAGAAATTTGAA
NM_001177842.1      CTTCGTGAAGCAGAGGAGAGCAGTAAGATCCGTGCTCCCACTACCATGAAGAAATTTGAA
                    ************************************************************

HsCD00043493        GATTCTGAAAAGGCCAAGAAACCTGTGAGGTCCATGATTGAGACACGGGGGGAAAAGCCC
NM_003139.3         GATTCTGAAAAGGCCAAGAAACCTGTGAGGTCCATGATTGAGACACGGGGGGAAAAGCCC
NM_001177842.1      GATTCTGAAAAGGCCAAGAAACCTGTGAGGTCCATGATTGAGACACGGGGGGAAAAGCCC
                    ************************************************************

HsCD00043493        AAGGAAAAAGCAAAGAATAGCAAAAAAAAGGGGGCCAAGAAGGAAGGTTCTGATGGTCCT
NM_003139.3         AAGGAAAAAGCAAAGAATAGCAAAAAAAAGGGGGCCAAGAAGGAAGGTTCTGATGGTCCT
NM_001177842.1      AAGGAAAAAGCAAAGAATAGCAAAAAAAAGGGGGCCAAGAAGGAAGGTTCTGATGGTCCT
                    ************************************************************

HsCD00043493        TTGGCTACCAGCAAACCAGTCCCTGCAGAAAAGTCAGGTCTTCCAGTGGGTCCTGAGAAC
NM_003139.3         TTGGCTACCAGCAAACCAGTCCCTGCAGAAAAGTCAGGTCTTCCAGTGGGTCCTGAGAAC
NM_001177842.1      TTGGCTACCAGCAAACCAGTCCCTGCAGAAAAGTCAGGTCTTCCAGTGGGTCCTGAGAAC
                    ************************************************************

HsCD00043493        GGAGTAGAACTTTCCAAAGAGGAGCTGATCCGCAGGAAGCGCGAGGAGTTCATTCAGAAG
NM_003139.3         GGAGTAGAACTTTCCAAAGAGGAGCTGATCCGCAGGAAGCGCGAGGAGTTCATTCAGAAG
NM_001177842.1      GGAGTAGAACTTTCCAAAGAGGAGCTGATCCGCAGGAAGCGCGAGGAGTTCATTCAGAAG
                    ************************************************************

HsCD00043493        CATGGGAGGGGTATGGAGAAGTCCAACAAGTCCACGAAGTCAGATGCTCCAAAGGAGAAG
NM_003139.3         CATGGGAGGGGTATGGAGAAGTCCAACAAGTCCACGAAGTCAGATGCTCCAAAGGAGAAG
NM_001177842.1      CATGGGAGGGGTATGGAGAAGTCCAACAAGTCCACGAAGTCAGATGCTCCAAAGGAGAAG
                    ************************************************************

HsCD00043493        GGCAAAAAAGCACCCCGGGTGTGGGAACTGGGTGGCTGTGCTAACAAAGAAGTGTTGGAT
NM_003139.3         GGCAAAAAAGCACCCCGGGTGTGGGAACTGGGTGGCTGTGCTAACAAAGAAGTGTTGGAT
NM_001177842.1      GGCAAAAAAGCACCCCGGGTGTGGGAACTGGGTGGCTGTGCTAACAAAGAAGTGTTGGAT
                    ************************************************************

HsCD00043493        TACAGTACTCCCACCACCAATGGAACCCCTGAGGCTGCCTTGTCTGAGGACATCAACCTG
NM_003139.3         TACAGTACTCCCACCACCAATGGAACCCCTGAGGCTGCCTTGTCTGAGGACATCAACCTG
NM_001177842.1      TACAGTACTCCCACCACCAATGGAACCCCTGAGGCTGCCTTGTCTGAGGACATCAACCTG
                    ************************************************************

HsCD00043493        ATTCGAGGGACTGGGTCTGGGGGGCAGCTTCAGGATCTGGACTGCAGCAGCTCTGATGAC
NM_003139.3         ATTCGAGGGACTGGGTCTGGGGGGCAGCTTCAGGATCTGGACTGCAGCAGCTCTGATGAC
NM_001177842.1      ATTCGAGGGACTGGGTCTGGGGGGCAGCTTCAGGATCTGGACTGCAGCAGCTCTGATGAC
                    ************************************************************

HsCD00043493        GAAGGGGCTGCTCAAAACTCTACCAAACCTAGTGCGACCAAGGGAACACTGGGTGGCATG
NM_003139.3         GAAGGGGCTGCTCAAAACTCTACCAAACCTAGTGCGACCAAGGGAACACTGGGTGGCATG
NM_001177842.1      GAAGGGGCTGCTCAAAACTCTACCAAACCTAGTGCGACCAAGGGAACACTGGGTGGCATG
                    ************************************************************

HsCD00043493        TTTGGTATGCTGAAGGGCCTTGTGGGTTCAAAGAGCTTGAGTCGTGAAGACATGGAATCT
NM_003139.3         TTTGGTATGCTGAAGGGCCTTGTGGGTTCAAAGAGCTTGAGTCGTGAAGACATGGAATCT
NM_001177842.1      TTTGGTATGCTGAAGGGCCTTGTGGGTTCAAAGAGCTTGAGTCGTGAAGACATGGAATCT
                    ************************************************************

HsCD00043493        GTGCTGGACAAGATGCGTGATCATCTCATTGCTAAGAACGTGGCTGCAGACATTGCCGTC
NM_003139.3         GTGCTGGACAAGATGCGTGATCATCTCATTGCTAAGAACGTGGCTGCAGACATTGCCGTC
NM_001177842.1      GTGCTGGACAAGATGCGTGATCATCTCATTGCTAAGAACGTGGCTGCAGACATTGCCGTC
                    ************************************************************

HsCD00043493        CAGCTCTGTGAATCTGTTGCCAACAAGTTGGAAGGGAAGGTGATGGGGACGTTCAGCACG
NM_003139.3         CAGCTCTGTGAATCTGTTGCCAACAAGTTGGAAGGGAAGGTGATGGGGACGTTCAGCACG
NM_001177842.1      CAGCTCTGTGAATCTGTTGCCAACAAGTTGGAAGGGAAGGTGATGGGGACGTTCAGCACG
                    ************************************************************

HsCD00043493        GTGACTTCCACAGTAAAGCAAGCCCTACAGGAGTCCCTGGTGCAGATTCTGCAGCCACAG
NM_003139.3         GTGACTTCCACAGTAAAGCAAGCCCTACAGGAGTCCCTGGTGCAGATTCTGCAGCCACAG
NM_001177842.1      GTGACTTCCACAGTAAAGCAAGCCCTACAGGAGTCCCTGGTGCAGATTCTGCAGCCACAG
                    ************************************************************

HsCD00043493        CGTCGTGTAGACATGCTCCGGGACATCATGGATGCCCAGCGTCGCCAGCGCCCTTATGTC
NM_003139.3         CGTCGTGTAGACATGCTCCGGGACATCATGGATGCCCAGCGTCGCCAGCGCCCTTATGTC
NM_001177842.1      CGTCGTGTAGACATGCTCCGGGACATCATGGATGCCCAGCGTCGCCAGCGCCCTTATGTC
                    ************************************************************

HsCD00043493        GTCACCTTCTGCGGCGTTAATGGAGTGGGGAAATCTACTAATCTTGCCAAGATTTCCTTC
NM_003139.3         GTCACCTTCTGCGGCGTTAATGGAGTGGGGAAATCTACTAATCTTGCCAAGATTTCCTTC
NM_001177842.1      GTCACCTTCTGCGGCGTTAATGGAGTGGGGAAATCTACTAATCTTGCCAAGATTTCCTTC
                    ************************************************************

HsCD00043493        TGGTTGTTAGAGAATGGCTTCAGTGTCCTCATTGCTGCCTGTGATACATTTCGTGCTGGG
NM_003139.3         TGGTTGTTAGAGAATGGCTTCAGTGTCCTCATTGCTGCCTGTGATACATTTCGTGCTGGG
NM_001177842.1      TGGTTGTTAGAGAATGGCTTCAGTGTCCTCATTGCTGCCTGTGATACATTTCGTGCTGGG
                    ************************************************************

HsCD00043493        GCCGTGGAGCAGCTGCGTACACACACCCGGCGTTTGAGTGCCCTACACCCTCCAGAGAAG
NM_003139.3         GCCGTGGAGCAGCTGCGTACACACACCCGGCGTTTGAGTGCCCTACACCCTCCAGAGAAG
NM_001177842.1      GCCGTGGAGCAGCTGCGTACACACACCCGGCGTTTGAGTGCCCTACACCCTCCAGAGAAG
                    ************************************************************

HsCD00043493        CATGGTGGCCGCACCATGGTGCAGTTGTTTGAAAAGGGCTATGGCAAGGATGCTGCTGGC
NM_003139.3         CATGGTGGCCGCACCATGGTGCAGTTGTTTGAAAAGGGCTATGGCAAGGATGCTGCTGGC
NM_001177842.1      CATGGTGGCCGCACCATGGTGCAGTTGTTTGAAAAGGGCTATGGCAAGGATGCTGCTGGC
                    ************************************************************

HsCD00043493        ATTGCCATGGAAGCCATTGCTTTTGCACGTAACCAAGGCTTTGACGTGGTGCTGGTGGAC
NM_003139.3         ATTGCCATGGAAGCCATTGCTTTTGCACGTAACCAAGGCTTTGACGTGGTGCTGGTGGAC
NM_001177842.1      ATTGCCATGGAAGCCATTGCTTTTGCACGTAACCAAGGCTTTGACGTGGTGCTGGTGGAC
                    ************************************************************

HsCD00043493        ACGGCAGGCCGCATGCAAGACAATGCCCCTCTGATGACTGCCCTGGCCAAACTCATTACT
NM_003139.3         ACGGCAGGCCGCATGCAAGACAATGCCCCTCTGATGACTGCCCTGGCCAAACTCATTACT
NM_001177842.1      ACGGCAGGCCGCATGCAAGACAATGCCCCTCTGATGACTGCCCTGGCCAAACTCATTACT
                    ************************************************************

HsCD00043493        GTCAATACACCTGATTTGGTGCTGTTTGTAGGAGAAGCCTTAGTAGGCAATGAAGCCGTG
NM_003139.3         GTCAATACACCTGATTTGGTGCTGTTTGTAGGAGAAGCCTTAGTAGGCAATGAAGCCGTG
NM_001177842.1      GTCAATACACCTGATTTGGTGCTGTTTGTAGGAGAAGCCTTAGTAGGCAATGAAGCCGTG
                    ************************************************************

HsCD00043493        GACCAGCTGGTCAAGTTCAACAGAGCCTTGGCTGACCATTCTATGGCTCAGACACCTCGG
NM_003139.3         GACCAGCTGGTCAAGTTCAACAGAGCCTTGGCTGACCATTCTATGGCTCAGACACCTCGG
NM_001177842.1      GACCAGCTGGTCAAGTTCAACAGAGCCTTGGCTGACCATTCTATGGCTCAGACACCTCGG
                    ************************************************************

HsCD00043493        CTCATTGATGGCATTGTTCTTACCAAATTTGATACCATTGATGACAAGGTGGGAGCTGCT
NM_003139.3         CTCATTGATGGCATTGTTCTTACCAAATTTGATACCATTGATGACAAGGTGGGAGCTGCT
NM_001177842.1      CTCATTGATGGCATTGTTCTTACCAAATTTGATACCATTGATGACAAGGTGGGAGCTGCT
                    ************************************************************

HsCD00043493        ATTTCTATGACGTACATCACAAGCAAACCCATCGTCTTTGTGGGCACCGGCCAGACCTAC
NM_003139.3         ATTTCTATGACGTACATCACAAGCAAACCCATCGTCTTTGTGGGCACCGGCCAGACCTAC
NM_001177842.1      ATTTCTATGACGTACATCACAAGCAAACCCATCGTCTTTGTGGGCACCGGCCAGACCTAC
                    ************************************************************

HsCD00043493        TGTGACCTACGCAGCCTCAATGCCAAGGCTGTGGTGGCTGCCCTCATGAAGGCTTAG
NM_003139.3         TGTGACCTACGCAGCCTCAATGCCAAGGCTGTGGTGGCTGCCCTCATGAAGGCTTAA
NM_001177842.1      TGTGACCTACGCAGCCTCAATGCCAAGGCTGTGGTGGCTGCCCTCATGAAGGCTTAA
                    ********************************************************.

Pathways

Reactome : Gene Expression
Reactome : IRE1alpha activates chaperones
Reactome : Metabolism of proteins
Reactome : SRP-dependent cotranslational protein targeting to membrane
Reactome : Translation
Reactome : Unfolded Protein Response (UPR)
Reactome : XBP1(S) activates chaperone genes

Clone

Cloning Information : 177318
HIP Master Clone ID : 152463
Original Clone ID : FLH177318.01X

Organism


TAX_ID : 9606
Species Specific ID: 6734

Genome

Chromosome : 11
Map Location : 11q24.2
Ensembl : ENSG00000182934

Reagents

Labome : SRPR-antibody