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OPN3 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  OPN3
Gene Name:  opsin 3
Sequence             
Original Clone ID: ccsbBroad304_02806
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00435090

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1207nts         Open reading frame : 1 to 1207
Reference Sequence Alignment

ATGTACTCGGGGAACCGCAGCGGCGGCCACGGCTACTGGGACGGCGGCGGGGCCGCGGGC
GCTGAGGGGCCGGCGCCGGCGGGGACACTGAGCCCCGCGCCCCTCTTCAGCCCCGGCACC
TACGAGCGCCTGGCGCTGCTGCTGGGCTCCATTGGGCTGCTGGGCGTCGGCAACAACCTG
CTGGTGCTCGTCCTCTACTACAAGTTCCAGCGGCTCCGCACTCCCACTCACCTCCTCCTG
GTCAACATCAGCCTCAGCGACCTGCTGGTGTCCCTCTTCGGGGTCACCTTTACCTTCGTG
TCCTGCCTGAGGAACGGCTGGGTGTGGGACACCGTGGGCTGCGTGTGGGACGGGTTTAGC
GGCAGCCTCTTCGGGATTGTTTCCATTGCCACCCTAACCGTGCTGGCCTATGAACGTTAC
ATTCGCGTGGTCCATGCCAGAGTGATCAATTTTTCCTGGGCCTGGAGGGCCATTACCTAC
ATCTGGCTCTACTCACTGGCGTGGGCAGGAGCACCTCTCCTGGGATGGAACAGGTACATC
CTGGACGTACACGGACTAGGCTGCACTGTGGACTGGAAATCCAAGGATGCCAACGATTCC
TCCTTTGTGCTTTTCTTATTTCTTGGCTGCCTGGTGGTGCCCCTGGGTGTCATAGCCCAT
TGCTATGGCCATATTCTATATTCCATTCGAATGCTTCGTTGTGTGGAAGATCTTCAGACA
ATTCAAGTGATCAAGATTTTAAAATATGAAAAGAAACTGGCCAAAATGTGCTTTTTAATG
ATATTCACCTTCCTGGTCTGTTGGATGCCTTATATCGTGATCTGCTTCTTGGTGGTTAAT
GGTCATGGTCACCTGGTCACTCCAACAATATCTATTGTTTCGTACCTCTTTGCTAAATCG
AACACTGTATACAATCCAGTGATTTATGTCTTCATGATCAGAAAGTTTCGAAGATCCCTT
TTGCAGCTTCTGTGCCTCCGACTGCTGAGGTGCCAGAGGCCTGCTAAAGACCTACCAGCA
GCTGGAAGTGAAATGCAGATCAGACCCATTGTGATGTCACAGAAAGATGGGGACAGGCCA
AAGAAAAAAGTGACTTTCAACTCTTCTTCCATCATTTTTATCATCACCAGTGATGAATCA
CTGTCAGTTGACGACAGCGACAAAACCAATGGGTCCAAAGTTGATGTAATCCAAGTTCGT
CCTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : OPN3
Symbol Nomenclature : OPN3
SYNONYM : ECPN
Designation : encephalopsin|opsin 3 (encephalopsin, panopsin)|opsin-3
Full Nomenclature : opsin 3
GENEID : 23596
HGNC : 14007
MIM : 606695
Vega : OTTHUMG00000039691
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00435090      ATGTACTCGGGGAACCGCAGCGGCGGCCACGGCTACTGGGACGGCGGCGGGGCCGCGGGC
NM_014322.2       ATGTACTCGGGGAACCGCAGCGGCGGCCACGGCTACTGGGACGGCGGCGGGGCCGCGGGC
                  ************************************************************

HsCD00435090      GCTGAGGGGCCGGCGCCGGCGGGGACACTGAGCCCCGCGCCCCTCTTCAGCCCCGGCACC
NM_014322.2       GCTGAGGGGCCGGCGCCGGCGGGGACACTGAGCCCCGCGCCCCTCTTCAGCCCCGGCACC
                  ************************************************************

HsCD00435090      TACGAGCGCCTGGCGCTGCTGCTGGGCTCCATTGGGCTGCTGGGCGTCGGCAACAACCTG
NM_014322.2       TACGAGCGCCTGGCGCTGCTGCTGGGCTCCATTGGGCTGCTGGGCGTCGGCAACAACCTG
                  ************************************************************

HsCD00435090      CTGGTGCTCGTCCTCTACTACAAGTTCCAGCGGCTCCGCACTCCCACTCACCTCCTCCTG
NM_014322.2       CTGGTGCTCGTCCTCTACTACAAGTTCCAGCGGCTCCGCACTCCCACTCACCTCCTCCTG
                  ************************************************************

HsCD00435090      GTCAACATCAGCCTCAGCGACCTGCTGGTGTCCCTCTTCGGGGTCACCTTTACCTTCGTG
NM_014322.2       GTCAACATCAGCCTCAGCGACCTGCTGGTGTCCCTCTTCGGGGTCACCTTTACCTTCGTG
                  ************************************************************

HsCD00435090      TCCTGCCTGAGGAACGGCTGGGTGTGGGACACCGTGGGCTGCGTGTGGGACGGGTTTAGC
NM_014322.2       TCCTGCCTGAGGAACGGCTGGGTGTGGGACACCGTGGGCTGCGTGTGGGACGGGTTTAGC
                  ************************************************************

HsCD00435090      GGCAGCCTCTTCGGGATTGTTTCCATTGCCACCCTAACCGTGCTGGCCTATGAACGTTAC
NM_014322.2       GGCAGCCTCTTCGGGATTGTTTCCATTGCCACCCTAACCGTGCTGGCCTATGAACGTTAC
                  ************************************************************

HsCD00435090      ATTCGCGTGGTCCATGCCAGAGTGATCAATTTTTCCTGGGCCTGGAGGGCCATTACCTAC
NM_014322.2       ATTCGCGTGGTCCATGCCAGAGTGATCAATTTTTCCTGGGCCTGGAGGGCCATTACCTAC
                  ************************************************************

HsCD00435090      ATCTGGCTCTACTCACTGGCGTGGGCAGGAGCACCTCTCCTGGGATGGAACAGGTACATC
NM_014322.2       ATCTGGCTCTACTCACTGGCGTGGGCAGGAGCACCTCTCCTGGGATGGAACAGGTACATC
                  ************************************************************

HsCD00435090      CTGGACGTACACGGACTAGGCTGCACTGTGGACTGGAAATCCAAGGATGCCAACGATTCC
NM_014322.2       CTGGACGTACACGGACTAGGCTGCACTGTGGACTGGAAATCCAAGGATGCCAACGATTCC
                  ************************************************************

HsCD00435090      TCCTTTGTGCTTTTCTTATTTCTTGGCTGCCTGGTGGTGCCCCTGGGTGTCATAGCCCAT
NM_014322.2       TCCTTTGTGCTTTTCTTATTTCTTGGCTGCCTGGTGGTGCCCCTGGGTGTCATAGCCCAT
                  ************************************************************

HsCD00435090      TGCTATGGCCATATTCTATATTCCATTCGAATGCTTCGTTGTGTGGAAGATCTTCAGACA
NM_014322.2       TGCTATGGCCATATTCTATATTCCATTCGAATGCTTCGTTGTGTGGAAGATCTTCAGACA
                  ************************************************************

HsCD00435090      ATTCAAGTGATCAAGATTTTAAAATATGAAAAGAAACTGGCCAAAATGTGCTTTTTAATG
NM_014322.2       ATTCAAGTGATCAAGATTTTAAAATATGAAAAGAAACTGGCCAAAATGTGCTTTTTAATG
                  ************************************************************

HsCD00435090      ATATTCACCTTCCTGGTCTGTTGGATGCCTTATATCGTGATCTGCTTCTTGGTGGTTAAT
NM_014322.2       ATATTCACCTTCCTGGTCTGTTGGATGCCTTATATCGTGATCTGCTTCTTGGTGGTTAAT
                  ************************************************************

HsCD00435090      GGTCATGGTCACCTGGTCACTCCAACAATATCTATTGTTTCGTACCTCTTTGCTAAATCG
NM_014322.2       GGTCATGGTCACCTGGTCACTCCAACAATATCTATTGTTTCGTACCTCTTTGCTAAATCG
                  ************************************************************

HsCD00435090      AACACTGTATACAATCCAGTGATTTATGTCTTCATGATCAGAAAGTTTCGAAGATCCCTT
NM_014322.2       AACACTGTATACAATCCAGTGATTTATGTCTTCATGATCAGAAAGTTTCGAAGATCCCTT
                  ************************************************************

HsCD00435090      TTGCAGCTTCTGTGCCTCCGACTGCTGAGGTGCCAGAGGCCTGCTAAAGACCTACCAGCA
NM_014322.2       TTGCAGCTTCTGTGCCTCCGACTGCTGAGGTGCCAGAGGCCTGCTAAAGACCTACCAGCA
                  ************************************************************

HsCD00435090      GCTGGAAGTGAAATGCAGATCAGACCCATTGTGATGTCACAGAAAGATGGGGACAGGCCA
NM_014322.2       GCTGGAAGTGAAATGCAGATCAGACCCATTGTGATGTCACAGAAAGATGGGGACAGGCCA
                  ************************************************************

HsCD00435090      AAGAAAAAAGTGACTTTCAACTCTTCTTCCATCATTTTTATCATCACCAGTGATGAATCA
NM_014322.2       AAGAAAAAAGTGACTTTCAACTCTTCTTCCATCATTTTTATCATCACCAGTGATGAATCA
                  ************************************************************

HsCD00435090      CTGTCAGTTGACGACAGCGACAAAACCAATGGGTCCAAAGTTGATGTAATCCAAGTTCGT
NM_014322.2       CTGTCAGTTGACGACAGCGACAAAACCAATGGGTCCAAAGTTGATGTAATCCAAGTTCGT
                  ************************************************************

HsCD00435090      CCTTTG---
NM_014322.2       CCTTTGTAG
                  ******   

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (i) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Opsins
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class A Rhodopsin-like

Protein

HPRD : 05985

Clone

Original Clone ID : ccsbBroad304_02806

Organism


TAX_ID : 9606
Species Specific ID: 23596

Genome

Chromosome : 1
Map Location : 1q43
Ensembl : ENSG00000054277

Reagents

Labome : OPN3-antibody