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PLTP (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PLTP
Gene Name:  phospholipid transfer protein
Sequence             
Original Clone ID: ccsbBroad304_01224
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00435217

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1324nts         Open reading frame : 1 to 1324
Reference Sequence Alignment

ATGGCCCTCTTCGGGGCCCTCTTCCTAGCGCTGCTGGCAGGCGCACATGCAGAGTTCCCA
GGCTGCAAGATCCGCGTCACCTCCAAGGCGCTGGAGCTGGTGAAGCAGGAGGGGCTGCGC
TTTCTGGAGCAAGAGCTGGAGACTATCACCATTCCGGACCTGCGGGGCAAAGAAGGCCAC
TTCTACTACAACATCTCTGAGGTGAAGGTCACAGAGCTGCAACTGACATCTTCCGAGCTC
GATTTCCAGCCACAGCAGGAGCTGATGCTTCAAATCACCAATGCCTCCTTGGGGCTGCGC
TTCCGGAGACAGCTGCTCTACTGGTTCTTGAAGGTGTATGATTTTCTCTCCACGTTCATC
ACCTCAGGGATGCGCTTCCTCCTCAACCAGCAGATCTGCCCTGTCCTCTACCACGCAGGG
ACGGTCCTGCTCAACTCCCTCCTGGACACCGTGCCTGTGCGCAGTTCTGTGGACGAGCTT
GTTGGCATTGACTATTCCCTCATGAAGGATCCTGTGGCTTCCACCAGCAACCTGGACATG
GACTTCCGGGGGGCCTTCTTCCCCCTGACTGAGAGGAACTGGAGCCTCCCCAACCGGGCA
GTGGAGCCCCAGCTGCAGGAGGAAGAGCGGATGGTGTATGTGGCCTTCTCTGAGTTCTTC
TTCGACTCTGCCATGGAGAGCTACTTCCGGGCGGGGGCCCTGCAGCTGTTGCTGGTGGGG
GACAAGGTGCCCCACGACCTGGACATGCTGCTGAGGGCCACCTACTTTGGGAGCATTGTC
CTGCTGAGCCCAGCAGTGATTGACTCCCCATTGAAGCTGGAGCTGCGGGTCCTGGCCCCA
CCGCGCTGCACCATCAAGCCCTCTGGCACCACCATCTCTGTCACTGCTAGCGTCACCATT
GCCCTGGTCCCACCAGACCAGCCTGAGGTCCAGCTGTCCAGCATGACTATGGACGCCCGT
CTCAGCGCCAAGATGGCTCTCCGGGGGAAGGCCCTGCGCACGCAGCTGGACCTGCGCAGG
TTCCGAATCTATTCCAACCATTCTGCACTGGAGTCGCTGGCTCTGATCCCATTACAGGCC
CCTCTGAAGACCATGCTGCAGATTGGGGTGATGCCCATGCTCAATGAGCGGACCTGGCGT
GGGGTGCAGATCCCACTACCTGAGGGCATCAACTTTGTGCATGAGGTGGTGACGAACCAT
GCGGGATTCCTCACCATCGGGGCTGATCTCCACTTTGCCAAAGGGCTGCGAGAGGTGATT
GAGAAGAACCGGCCTGCTGATGTCAGGGCGTCCACTGCCCCCACACCGTCCACAGCAGCT
GTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PLTP
Symbol Nomenclature : PLTP
SYNONYM : BPIFE; HDLCQ9
Designation : BPI fold containing family E|lipid transfer protein II
Full Nomenclature : phospholipid transfer protein
GENEID : 5360
GenBank Accession : DQ894981
HGNC : 9093
MIM : 172425
Vega : OTTHUMG00000033047
Target GenBank: DQ894981

 

Reference Sequence Alignment

  

HsCD00435217      ATGGCCCTCTTCGGGGCCCTCTTCCTAGCGCTGCTGGCAGGCGCACATGCAGAGTTCCCA
NM_182676.2       ATGGCCCTCTTCGGGGCCCTCTTCCTAGCGCTGCTGGCAGGCGCACATGCAGAGTTCCCA
                  ************************************************************

HsCD00435217      GGCTGCAAGATCCGCGTCACCTCCAAGGCGCTGGAGCTGGTGAAGCAGGAGGGGCTGCGC
NM_182676.2       GGCTGCAAGATCCGCGTCACCTCCAAGGCGCTGGAGCTGGTGAAGCAGGAGGGGCTGCGC
                  ************************************************************

HsCD00435217      TTTCTGGAGCAAGAGCTGGAGACTATCACCATTCCGGACCTGCGGGGCAAAGAAGGCCAC
NM_182676.2       TTTCTGGAGCAAGAGCTGGAGACTATCACCATTCCGGACCTGCGGGGCAAAGAAGGCCAC
                  ************************************************************

HsCD00435217      TTCTACTACAACATCTCTGAGGTGAAGGTCACAGAGCTGCAACTGACATCTTCCGAGCTC
NM_182676.2       TTCTACTACAACATCTCTGAGGTGAAGGTCACAGAGCTGCAACTGACATCTTCCGAGCTC
                  ************************************************************

HsCD00435217      GATTTCCAGCCACAGCAGGAGCTGATGCTTCAAATCACCAATGCCTCCTTGGGGCTGCGC
NM_182676.2       GATTTCCAGCCACAGCAGGAGCTGATGCTTCAAATCACCAATGCCTCCTTGGGGCTGCGC
                  ************************************************************

HsCD00435217      TTCCGGAGACAGCTGCTCTACTGGTTCTTGAAGGTGTATGATTTTCTCTCCACGTTCATC
NM_182676.2       TTCCGGAGACAGCTGCTCTACTGGTTCTTGAAGGTGTATGATTTTCTCTCCACGTTCATC
                  ************************************************************

HsCD00435217      ACCTCAGGGATGCGCTTCCTCCTCAACCAGCAGATCTGCCCTGTCCTCTACCACGCAGGG
NM_182676.2       ACCTCAGGGATGCGCTTCCTCCTCAACCAGCAGATCTGCCCTGTCCTCTACCACGCAGGG
                  ************************************************************

HsCD00435217      ACGGTCCTGCTCAACTCCCTCCTGGACACCGTGCCTGTGCGCAGTTCTGTGGACGAGCTT
NM_182676.2       ACGGTCCTGCTCAACTCCCTCCTGGACACCGTGCCTGTGCGCAGTTCTGTGGACGAGCTT
                  ************************************************************

HsCD00435217      GTTGGCATTGACTATTCCCTCATGAAGGATCCTGTGGCTTCCACCAGCAACCTGGACATG
NM_182676.2       GTTGGCATTGACTATTCCCTCATGAAGGATCCTGTGGCTTCCACCAGCAACCTGGACATG
                  ************************************************************

HsCD00435217      GACTTCCGGGGGGCCTTCTTCCCCCTGACTGAGAGGAACTGGAGCCTCCCCAACCGGGCA
NM_182676.2       GACTTCCGGGGGGCCTTCTTCCCCCTGACTGAGAGGAACTGGAGCCTCCCCAACCGGGCA
                  ************************************************************

HsCD00435217      GTGGAGCCCCAGCTGCAGGAGGAAGAGCGGATGGTGTATGTGGCCTTCTCTGAGTTCTTC
NM_182676.2       GTGGAGCCCCAGCTGCAGGAGGAAGAGCGGATGGTGTATGTGGCCTTCTCTGAGTTCTTC
                  ************************************************************

HsCD00435217      TTCGACTCTGCCATGGAGAGCTACTTCCGGGCGGGGGCCCTGCAGCTGTTGCTGGTGGGG
NM_182676.2       TTCGACTCTGCCATGGAGAGCTACTTCCGGGCGGGGGCCCTGCAGCTGTTGCTGGTGGGG
                  ************************************************************

HsCD00435217      GACAAGGTGCCCCACGACCTGGACATGCTGCTGAGGGCCACCTACTTTGGGAGCATTGTC
NM_182676.2       GACAAGGTGCCCCACGACCTGGACATGCTGCTGAGGGCCACCTACTTTGGGAGCATTGTC
                  ************************************************************

HsCD00435217      CTGCTGAGCCCAGCAGTGATTGACTCCCCATTGAAGCTGGAGCTGCGGGTCCTGGCCCCA
NM_182676.2       CTGCTGAGCCCAGCAGTGATTGACTCCCCATTGAAGCTGGAGCTGCGGGTCCTGGCCCCA
                  ************************************************************

HsCD00435217      CCGCGCTGCACCATCAAGCCCTCTGGCACCACCATCTCTGTCACTGCTAGCGTCACCATT
NM_182676.2       CCGCGCTGCACCATCAAGCCCTCTGGCACCACCATCTCTGTCACTGCTAGCGTCACCATT
                  ************************************************************

HsCD00435217      GCCCTGGTCCCACCAGACCAGCCTGAGGTCCAGCTGTCCAGCATGACTATGGACGCCCGT
NM_182676.2       GCCCTGGTCCCACCAGACCAGCCTGAGGTCCAGCTGTCCAGCATGACTATGGACGCCCGT
                  ************************************************************

HsCD00435217      CTCAGCGCCAAGATGGCTCTCCGGGGGAAGGCCCTGCGCACGCAGCTGGACCTGCGCAGG
NM_182676.2       CTCAGCGCCAAGATGGCTCTCCGGGGGAAGGCCCTGCGCACGCAGCTGGACCTGCGCAGG
                  ************************************************************

HsCD00435217      TTCCGAATCTATTCCAACCATTCTGCACTGGAGTCGCTGGCTCTGATCCCATTACAGGCC
NM_182676.2       TTCCGAATCTATTCCAACCATTCTGCACTGGAGTCGCTGGCTCTGATCCCATTACAGGCC
                  ************************************************************

HsCD00435217      CCTCTGAAGACCATGCTGCAGATTGGGGTGATGCCCATGCTCAATGAGCGGACCTGGCGT
NM_182676.2       CCTCTGAAGACCATGCTGCAGATTGGGGTGATGCCCATGCTCAATGAGCGGACCTGGCGT
                  ************************************************************

HsCD00435217      GGGGTGCAGATCCCACTACCTGAGGGCATCAACTTTGTGCATGAGGTGGTGACGAACCAT
NM_182676.2       GGGGTGCAGATCCCACTACCTGAGGGCATCAACTTTGTGCATGAGGTGGTGACGAACCAT
                  ************************************************************

HsCD00435217      GCGGGATTCCTCACCATCGGGGCTGATCTCCACTTTGCCAAAGGGCTGCGAGAGGTGATT
NM_182676.2       GCGGGATTCCTCACCATCGGGGCTGATCTCCACTTTGCCAAAGGGCTGCGAGAGGTGATT
                  ************************************************************

HsCD00435217      GAGAAGAACCGGCCTGCTGATGTCAGGGCGTCCACTGCCCCCACACCGTCCACAGCAGCT
NM_182676.2       GAGAAGAACCGGCCTGCTGATGTCAGGGCGTCCACTGCCCCCACACCGTCCACAGCAGCT
                  ************************************************************

HsCD00435217      GTC---
NM_182676.2       GTCTGA
                  ***   

Pathways

Reactome : HDL-mediated lipid transport
Reactome : Lipid digestion, mobilization, and transport
Reactome : Lipoprotein metabolism
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
WikiPathway : Statin Pathway

Clone

Original Clone ID : ccsbBroad304_01224

Organism


TAX_ID : 9606
Species Specific ID: 5360

Genome

Chromosome : 20
Map Location : 20q13.12
Ensembl : ENSG00000100979