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CRISPLD1 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CRISPLD1
Gene Name:  cysteine-rich secretory protein LCCL domain containing 1
Sequence             
Original Clone ID: ccsbBroad304_09106
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00435230

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1501nts         Open reading frame : 1 to 1501
Reference Sequence Alignment

ATGAAGTGTACCGCTCGGGAGTGGCTCAGAGTAACCACAGTGCTGTTCATGGCTAGAGCA
ATTCCAGCCATGGTGGTTCCCAATGCCACTTTATTGGAGAAACTTTTGGAAAAATACATG
GATGAGGATGGTGAGTGGTGGATAGCCAAACAACGAGGGAAAAGGGCCATCACAGACAAT
GACATGCAGAGTATTTTGGACCTTCATAATAAATTACGAAGTCAGGTGTATCCAACAGCC
TCTAATATGGAGTATATGACATGGGATGTAGAGCTGGAAAGATCTGCAGAATCCTGGGCT
GAAAGTTGCTTGTGGGAACATGGACCTGCAAGCTTGCTTCCATCAATTGGACAGAATTTG
GGAGCACACTGGGGAAGATATAGGCCCCCGACGTTTCATGTACAATCGTGGTATGATGAA
GTGAAAGACTTTAGCTACCCATATGAACATGAATGCAACCCATATTGTCCATTCAGGTGT
TCTGGCCCTGTATGTACACATTATACACAGGTCGTGTGGGCAACTAGTAACAGAATCGGT
TGTGCCATTAATTTGTGTCATAACATGAACATCTGGGGGCAGATATGGCCCAAAGCTGTC
TACCTGGTGTGCAATTACTCCCCAAAGGGAAACTGGTGGGGCCATGCCCCTTACAAACAT
GGGCGGCCCTGTTCTGCTTGCCCACCTAGTTTTGGAGGGGGCTGTAGAGAAAATCTGTGC
TACAAAGAAGGGTCAGACAGGTATTATCCCCCTCGAGAAGAGGAAACAAATGAAATAGAA
CGACAGCAGTCACAAGTCCATGACACCCATGTCCGGACAAGATCAGATGATAGTAGCAGA
AATGAAGTCATAAGCGCACAGCAAATGTCCCAAATTGTTTCTTGTGAAGTAAGATTAAGA
GATCAGTGCAAAGGAACAACCTGCAATAGGTACGAATGTCCTGCTGGCTGTTTGGATAGT
AAAGCTAAAGTTATTGGCAGTGTACATTATGAAATGCAATCCAGCATCTGTAGAGCTGCA
ATTCATTATGGTATAATAGACAATGATGGTGGCTGGGTAGATATCACTAGACAAGGAAGA
AAGCATTATTTCATCAAGTCCAATAGAAATGGTATTCAAACAATTGGCAAATATCAGTCT
GCTAATTCCTTCACAGTCTCTAAAGTAACAGTTCAGGCTGTGACTTGTGAAACAACTGTG
GAACAGCTCTGTCCATTTCATAAGCCTGCTTCACATTGCCCAAGAGTATACTGTCCTCGT
AACTGTATGCAAGCAAATCCACATTATGCTCGTGTAATTGGAACTCGAGTTTATTCTGAT
CTGTCCAGTATCTGCAGAGCAGCAGTACATGCTGGAGTGGTTCGAAATCACGGTGGTTAT
GTTGATGTAATGCCTGTGGACAAAAGAAAGACCTACATTGCTTCTTTTCAGAATGGAATC
TTCTCAGAAAGTTTACAGAATCCTCCAGGAGGAAAGGCATTCAGAGTGTTTGCTGTTGTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
1 error
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  None
Description: with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: EvNO00877063
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CRISPLD1
Symbol Nomenclature : CRISPLD1
SYNONYM : CRISP-10; CRISP10; LCRISP1
Designation : CocoaCrisp|LCCL domain containing cysteine-rich secretory protein 1|LCCL domain-containing cysteine-rich secretory protein 1|cysteine-rich secretory protein 10|cysteine-rich secretory protein LCCL domain-containing 1|trypsin inhibitor Hl
Full Nomenclature : cysteine-rich secretory protein LCCL domain containing 1
GENEID : 83690
Locus Tag : UNQ342/PRO541
GenBank Accession : JF432689
HGNC : 18206
Vega : OTTHUMG00000164529
Target GenBank: JF432689

 

Reference Sequence Alignment

  

HsCD00435230      ATGAAGTGTACCGCTCGGGAGTGGCTCAGAGTAACCACAGTGCTGTTCATGGCTAGAGCA
NM_031461.5       ATGAAGTGTACCGCGCGGGAGTGGCTCAGAGTAACCACAGTGCTGTTCATGGCTAGAGCA
                  ************** *********************************************

HsCD00435230      ATTCCAGCCATGGTGGTTCCCAATGCCACTTTATTGGAGAAACTTTTGGAAAAATACATG
NM_031461.5       ATTCCAGCCATGGTGGTTCCCAATGCCACTTTATTGGAGAAACTTTTGGAAAAATACATG
                  ************************************************************

HsCD00435230      GATGAGGATGGTGAGTGGTGGATAGCCAAACAACGAGGGAAAAGGGCCATCACAGACAAT
NM_031461.5       GATGAGGATGGTGAGTGGTGGATAGCCAAACAACGAGGGAAAAGGGCCATCACAGACAAT
                  ************************************************************

HsCD00435230      GACATGCAGAGTATTTTGGACCTTCATAATAAATTACGAAGTCAGGTGTATCCAACAGCC
NM_031461.5       GACATGCAGAGTATTTTGGACCTTCATAATAAATTACGAAGTCAGGTGTATCCAACAGCC
                  ************************************************************

HsCD00435230      TCTAATATGGAGTATATGACATGGGATGTAGAGCTGGAAAGATCTGCAGAATCCTGGGCT
NM_031461.5       TCTAATATGGAGTATATGACATGGGATGTAGAGCTGGAAAGATCTGCAGAATCCTGGGCT
                  ************************************************************

HsCD00435230      GAAAGTTGCTTGTGGGAACATGGACCTGCAAGCTTGCTTCCATCAATTGGACAGAATTTG
NM_031461.5       GAAAGTTGCTTGTGGGAACATGGACCTGCAAGCTTGCTTCCATCAATTGGACAGAATTTG
                  ************************************************************

HsCD00435230      GGAGCACACTGGGGAAGATATAGGCCCCCGACGTTTCATGTACAATCGTGGTATGATGAA
NM_031461.5       GGAGCACACTGGGGAAGATATAGGCCCCCGACGTTTCATGTACAATCGTGGTATGATGAA
                  ************************************************************

HsCD00435230      GTGAAAGACTTTAGCTACCCATATGAACATGAATGCAACCCATATTGTCCATTCAGGTGT
NM_031461.5       GTGAAAGACTTTAGCTACCCATATGAACATGAATGCAACCCATATTGTCCATTCAGGTGT
                  ************************************************************

HsCD00435230      TCTGGCCCTGTATGTACACATTATACACAGGTCGTGTGGGCAACTAGTAACAGAATCGGT
NM_031461.5       TCTGGCCCTGTATGTACACATTATACACAGGTCGTGTGGGCAACTAGTAACAGAATCGGT
                  ************************************************************

HsCD00435230      TGTGCCATTAATTTGTGTCATAACATGAACATCTGGGGGCAGATATGGCCCAAAGCTGTC
NM_031461.5       TGTGCCATTAATTTGTGTCATAACATGAACATCTGGGGGCAGATATGGCCCAAAGCTGTC
                  ************************************************************

HsCD00435230      TACCTGGTGTGCAATTACTCCCCAAAGGGAAACTGGTGGGGCCATGCCCCTTACAAACAT
NM_031461.5       TACCTGGTGTGCAATTACTCCCCAAAGGGAAACTGGTGGGGCCATGCCCCTTACAAACAT
                  ************************************************************

HsCD00435230      GGGCGGCCCTGTTCTGCTTGCCCACCTAGTTTTGGAGGGGGCTGTAGAGAAAATCTGTGC
NM_031461.5       GGGCGGCCCTGTTCTGCTTGCCCACCTAGTTTTGGAGGGGGCTGTAGAGAAAATCTGTGC
                  ************************************************************

HsCD00435230      TACAAAGAAGGGTCAGACAGGTATTATCCCCCTCGAGAAGAGGAAACAAATGAAATAGAA
NM_031461.5       TACAAAGAAGGGTCAGACAGGTATTATCCCCCTCGAGAAGAGGAAACAAATGAAATAGAA
                  ************************************************************

HsCD00435230      CGACAGCAGTCACAAGTCCATGACACCCATGTCCGGACAAGATCAGATGATAGTAGCAGA
NM_031461.5       CGACAGCAGTCACAAGTCCATGACACCCATGTCCGGACAAGATCAGATGATAGTAGCAGA
                  ************************************************************

HsCD00435230      AATGAAGTCATAAGCGCACAGCAAATGTCCCAAATTGTTTCTTGTGAAGTAAGATTAAGA
NM_031461.5       AATGAAGTCATAAGCGCACAGCAAATGTCCCAAATTGTTTCTTGTGAAGTAAGATTAAGA
                  ************************************************************

HsCD00435230      GATCAGTGCAAAGGAACAACCTGCAATAGGTACGAATGTCCTGCTGGCTGTTTGGATAGT
NM_031461.5       GATCAGTGCAAAGGAACAACCTGCAATAGGTACGAATGTCCTGCTGGCTGTTTGGATAGT
                  ************************************************************

HsCD00435230      AAAGCTAAAGTTATTGGCAGTGTACATTATGAAATGCAATCCAGCATCTGTAGAGCTGCA
NM_031461.5       AAAGCTAAAGTTATTGGCAGTGTACATTATGAAATGCAATCCAGCATCTGTAGAGCTGCA
                  ************************************************************

HsCD00435230      ATTCATTATGGTATAATAGACAATGATGGTGGCTGGGTAGATATCACTAGACAAGGAAGA
NM_031461.5       ATTCATTATGGTATAATAGACAATGATGGTGGCTGGGTAGATATCACTAGACAAGGAAGA
                  ************************************************************

HsCD00435230      AAGCATTATTTCATCAAGTCCAATAGAAATGGTATTCAAACAATTGGCAAATATCAGTCT
NM_031461.5       AAGCATTATTTCATCAAGTCCAATAGAAATGGTATTCAAACAATTGGCAAATATCAGTCT
                  ************************************************************

HsCD00435230      GCTAATTCCTTCACAGTCTCTAAAGTAACAGTTCAGGCTGTGACTTGTGAAACAACTGTG
NM_031461.5       GCTAATTCCTTCACAGTCTCTAAAGTAACAGTTCAGGCTGTGACTTGTGAAACAACTGTG
                  ************************************************************

HsCD00435230      GAACAGCTCTGTCCATTTCATAAGCCTGCTTCACATTGCCCAAGAGTATACTGTCCTCGT
NM_031461.5       GAACAGCTCTGTCCATTTCATAAGCCTGCTTCACATTGCCCAAGAGTATACTGTCCTCGT
                  ************************************************************

HsCD00435230      AACTGTATGCAAGCAAATCCACATTATGCTCGTGTAATTGGAACTCGAGTTTATTCTGAT
NM_031461.5       AACTGTATGCAAGCAAATCCACATTATGCTCGTGTAATTGGAACTCGAGTTTATTCTGAT
                  ************************************************************

HsCD00435230      CTGTCCAGTATCTGCAGAGCAGCAGTACATGCTGGAGTGGTTCGAAATCACGGTGGTTAT
NM_031461.5       CTGTCCAGTATCTGCAGAGCAGCAGTACATGCTGGAGTGGTTCGAAATCACGGTGGTTAT
                  ************************************************************

HsCD00435230      GTTGATGTAATGCCTGTGGACAAAAGAAAGACCTACATTGCTTCTTTTCAGAATGGAATC
NM_031461.5       GTTGATGTAATGCCTGTGGACAAAAGAAAGACCTACATTGCTTCTTTTCAGAATGGAATC
                  ************************************************************

HsCD00435230      TTCTCAGAAAGTTTACAGAATCCTCCAGGAGGAAAGGCATTCAGAGTGTTTGCTGTTGTG
NM_031461.5       TTCTCAGAAAGTTTACAGAATCCTCCAGGAGGAAAGGCATTCAGAGTGTTTGCTGTTGTG
                  ************************************************************

HsCD00435230      ---
NM_031461.5       TGA
                     

Clone

Original Clone ID : ccsbBroad304_09106

Organism


TAX_ID : 9606
Species Specific ID: 83690

Genome

Chromosome : 8
Map Location : 8q21.11
Ensembl : ENSG00000121005

Reagents

Labome : CRISPLD1-antibody