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TCTE1 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TCTE1
Gene Name:  t-complex-associated-testis-expressed 1
Sequence             
Original Clone ID: ccsbBroad304_09824
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00435260

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1504nts         Open reading frame : 1 to 1504
Reference Sequence Alignment

ATGCAGGATACCGTAACGACATCAGCATTGTTGGACCCCAGCCACTCCTCAGTCTCCACC
CAGGACAATTCCTCCACTGGAGGACACACTTCAAGCACAAGCCTACAGCTCTCAAAGCCT
TCAATCACACCAGTCCCTGCAAAGTCCAGGAACCCACGTCCCAGGGCCAATATCCGTCGG
ATGCGCCGGATCATTGCTGAGGATCCTGAGTGGTCACTGGCCATCGTGCCCCTCCTCACA
GAGCTCTGCATTCAGCACATTATCAGGAACTTCCAGAAAAACCCTATCCTGAAGCAGATG
CTCCCGGAACACCAGCAGAAGGTCCTGAACCACCTGTCCCCTGACCTACCACTGGCTGTG
ACCGCCAACCTGATAGACAGTGAGAACTACTGGCTCCGCTGCTGCATGCATCGCTGGCCC
GTGTGCCACGTGGCCCACCATGGCGGCAGCTGGAAACGCATGTTCTTCGAGCGGCACCTG
GAGAACCTGCTAAAGCACTTTATCCCAGGCACCACAGACCCTGCGGTGATCCTCGACCTG
CTGCCGCTCTGCCGGAATTACGTGCGCAGGGTCCACGTCGATCAGTTCCTTCCGCCGGTG
CAGCTCCCGGCCCAGCTCCGGCCGGGCGACCAGTCCGACTCAGGCAGCGAGGGAGAGATG
GAGGAGCCCACCGTTGACCACTACCAACTGGGCGATCTGGTAGCTGGCCTGAGCCACCTG
GAGGAGCTGGACCTGGTGTACGATGTCAAGGACTGCGGCATGAATTTCGAGTGGAATCTC
TTCCTCTTCACCTACCGCGACTGCCTCTCCTTGGCAGCCGCCATCAAGGCATGCCACACC
CTCAAGATCTTCAAGCTGACCCGAAGCAAGGTGGATGATGACAAGGCACGCATCATAATT
CGAAGCCTTCTGGACCACCCAGTCCTCGAGGAGCTGGACTTGTCACAAAACCTCATTGGA
GACCGTGGTGCACGAGGCGCTGCCAAGCTGCTGAGCCACAGCCGCCTGCGTGTGCTCAAC
CTGGCTAACAACCAGGTGCGTGCACCCGGTGCCCAGTCCCTGGCTCACGCTCTGGCACAC
AACACCAACCTCATTTCCCTCAACCTACGTCTCAACTGCATCGAGGATGAGGGTGGCCAG
GCTCTTGCCCATGCCTTGCAGACCAACAAGTGCCTCACCACGCTGCACCTCGGTGGCAAT
GAGCTGTCTGAGCCCACCGCCACACTCCTGTCACAGGTGCTCGCCATCAACACCACACTC
ACCAGCATCAACCTGTCCTGCAACCACATCGGGCTGGACGGTGGGAAGCAGCTCCTGGAA
GGCATGTCAGACAACAAGACCCTCCTGGAATTTGACTTGCGCCTGTCAGATGTGGCCCAG
GAAAGCGAGTACCTCATTGGCCAGGCCCTCTACGCAAACCGAGAAGCAGCCCGCCAGCGG
GCCCTGAATCCCAGCCACTTCATGTCAACCATCACTGCCAATGGCCCTGAGAACTCTGTG
GGA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TCTE1
Symbol Nomenclature : TCTE1
SYNONYM : D6S46
Designation : T-complex-associated testis-expressed protein 1|tcte-1
Full Nomenclature : t-complex-associated-testis-expressed 1
GENEID : 202500
Locus Tag : RP11-444E17.2
GenBank Accession : DQ894702
HGNC : 11693
MIM : 186975
Target GenBank: DQ894702

 

Reference Sequence Alignment

  

HsCD00435260        ATGCAGGATACCGTAACGACATCAGCATTGTTGGACCCCAGCCACTCCTCAGTCTCCACC
NM_182539.3         ATGCAGGATACCGTAACGACATCAGCATTGTTGGACCCCAGCCACTCCTCAGTCTCCACC
XM_005248872.1      ATGCAGGATACCGTAACGACATCAGCATTGTTGGACCCCAGCCACTCCTCAGTCTCCACC
                    ************************************************************

HsCD00435260        CAGGACAATTCCTCCACTGGAGGACACACTTCAAGCACAAGCCTACAGCTCTCAAAGCCT
NM_182539.3         CAGGACAATTCCTCCACTGGAGGACACACTTCAAGCACAAGCCCACAGCTCTCAAAGCCT
XM_005248872.1      CAGGACAATTCCTCCACTGGAGGACACACTTCAAGCACAAGCCCACAGCTCTCAAAGCCT
                    ******************************************* ****************

HsCD00435260        TCAATCACACCAGTCCCTGCAAAGTCCAGGAACCCACGTCCCAGGGCCAATATCCGTCGG
NM_182539.3         TCAATCACACCAGTCCCTGCAAAGTCCAGGAACCCACATCCCAGGGCCAATATCCGTCGG
XM_005248872.1      TCAATCACACCAGTCCCTGCAAAGTCCAGGAACCCACATCCCAGGGCCAATATCCGTCGG
                    *************************************.**********************

HsCD00435260        ATGCGCCGGATCATTGCTGAGGATCCTGAGTGGTCACTGGCCATCGTGCCCCTCCTCACA
NM_182539.3         ATGCGCCGGATCATTGCTGAGGATCCTGAGTGGTCACTGGCCATCGTGCCCCTCCTCACA
XM_005248872.1      ATGCGCCGGATCATTGCTGAGGATCCTGAGTGGTCACTGGCCATCGTGCCCCTCCTCACA
                    ************************************************************

HsCD00435260        GAGCTCTGCATTCAGCACATTATCAGGAACTTCCAGAAAAACCCTATCCTGAAGCAGATG
NM_182539.3         GAGCTCTGCATTCAGCACATTATCAGGAACTTCCAGAAAAACCCTATCCTGAAGCAGATG
XM_005248872.1      GAGCTCTGCATTCAGCACATTATCAGGAACTTCCAGAAAAACCCTATCCTGAAGCAGATG
                    ************************************************************

HsCD00435260        CTCCCGGAACACCAGCAGAAGGTCCTGAACCACCTGTCCCCTGACCTACCACTGGCTGTG
NM_182539.3         CTCCCGGAACACCAGCAGAAGGTCCTGAACCACCTGTCCCCTGACCTACCACTGGCTGTG
XM_005248872.1      CTCCCGGAACACCAGCAGAAGGTCCTGAACCACCTGTCCCCTGACCTACCACTGGCTGTG
                    ************************************************************

HsCD00435260        ACCGCCAACCTGATAGACAGTGAGAACTACTGGCTCCGCTGCTGCATGCATCGCTGGCCC
NM_182539.3         ACCGCCAACCTGATAGACAGTGAGAACTACTGGCTCCGCTGCTGCATGCATCGCTGGCCC
XM_005248872.1      ACCGCCAACCTGATAGACAGTGAGAACTACTGGCTCCGCTGCTGCATGCATCGCTGGCCC
                    ************************************************************

HsCD00435260        GTGTGCCACGTGGCCCACCATGGCGGCAGCTGGAAACGCATGTTCTTCGAGCGGCACCTG
NM_182539.3         GTGTGCCACGTGGCCCACCATGGCGGCAGCTGGAAACGCATGTTCTTCGAGCGGCACCTG
XM_005248872.1      GTGTGCCACGTGGCCCACCATGGCGGCAGCTGGAAACGCATGTTCTTCGAGCGGCACCTG
                    ************************************************************

HsCD00435260        GAGAACCTGCTAAAGCACTTTATCCCAGGCACCACAGACCCTGCGGTGATCCTCGACCTG
NM_182539.3         GAGAACCTGCTAAAGCACTTTATCCCAGGCACCACAGACCCTGCGGTGATCCTCGACCTG
XM_005248872.1      GAGAACCTGCTAAAGCACTTTATCCCAGGCACCACAGACCCTGCGGTGATCCTCGACCTG
                    ************************************************************

HsCD00435260        CTGCCGCTCTGCCGGAATTACGTGCGCAGGGTCCACGTCGATCAGTTCCTTCCGCCGGTG
NM_182539.3         CTGCCGCTCTGCCGGAATTACGTGCGCAGGGTCCACGTCGATCAGTTCCTTCCGCCGGTG
XM_005248872.1      CTGCCGCTCTGCCGGAATTACGTGCGCAGGGTCCACGTCGATCAGTTCCTTCCGCCGGTG
                    ************************************************************

HsCD00435260        CAGCTCCCGGCCCAGCTCCGGCCGGGCGACCAGTCCGACTCAGGCAGCGAGGGAGAGATG
NM_182539.3         CAGCTCCCGGCCCAGCTCCGGCCGGGCGACCAGTCCGACTCAGGCAGCGAGGGAGAGATG
XM_005248872.1      CAGCTCCCGGCCCAGCTCCGGCCGGGCGACCAGTCCGACTCAGGCAGCGAGGGAGAGATG
                    ************************************************************

HsCD00435260        GAGGAGCCCACCGTTGACCACTACCAACTGGGCGATCTGGTAGCTGGCCTGAGCCACCTG
NM_182539.3         GAGGAGCCCACCGTTGACCACTACCAACTGGGCGATCTGGTAGCTGGCCTGAGCCACCTG
XM_005248872.1      GAGGAGCCCACCGTTGACCACTACCAACTGGGCGATCTGGTAGCTGGCCTGAGCCACCTG
                    ************************************************************

HsCD00435260        GAGGAGCTGGACCTGGTGTACGATGTCAAGGACTGCGGCATGAATTTCGAGTGGAATCTC
NM_182539.3         GAGGAGCTGGACCTGGTGTACGATGTCAAGGACTGCGGCATGAATTTCGAGTGGAATCTC
XM_005248872.1      GAGGAGCTGGACCTGGTGTACGATGTCAAGGACTGCGGCATGAATTTCGAGTGGAATCTC
                    ************************************************************

HsCD00435260        TTCCTCTTCACCTACCGCGACTGCCTCTCCTTGGCAGCCGCCATCAAGGCATGCCACACC
NM_182539.3         TTCCTCTTCACCTACCGTGACTGCCTCTCCTTGGCAGCCGCCATCAAGGCATGCCACACC
XM_005248872.1      TTCCTCTTCACCTACCGTGACTGCCTCTCCTTGGCAGCCGCCATCAAGGCATGCCACACC
                    ***************** ******************************************

HsCD00435260        CTCAAGATCTTCAAGCTGACCCGAAGCAAGGTGGATGATGACAAGGCACGCATCATAATT
NM_182539.3         CTCAAGATCTTCAAGCTGACCCGAAGCAAGGTGGATGATGACAAGGCACGCATCATAATT
XM_005248872.1      CTCAAGATCTTCAAGCTGACCCGAAGCAAGGTGGATGATGACAAGGCACGCATCATAATT
                    ************************************************************

HsCD00435260        CGAAGCCTTCTGGACCACCCAGTCCTCGAGGAGCTGGACTTGTCACAAAACCTCATTGGA
NM_182539.3         CGAAGCCTTCTGGACCACCCAGTCCTCGAGGAGCTGGACTTGTCACAAAACCTCATTGGA
XM_005248872.1      CGAAGCCTTCTGGACCACCCAGTCCTCGAGGAGCTGGACTTGTCACAAAACCTCATTGGA
                    ************************************************************

HsCD00435260        GACCGTGGTGCACGAGGCGCTGCCAAGCTGCTGAGCCACAGCCGCCTGCGTGTGCTCAAC
NM_182539.3         GACCGTGGTGCACGAGGTGCTGCCAAGCTGCTGAGCCACAGCCGCCTGCGTGTGCTCAAC
XM_005248872.1      GACCGTGGTGCACGAGGTGCTGCCAAGCTGCTGAGCCACAGCCGCCTGCGTGTGCTCAAC
                    ***************** ******************************************

HsCD00435260        CTGGCTAACAACCAGGTGCGTGCACCCGGTGCCCAGTCCCTGGCTCACGCTCTGGCACAC
NM_182539.3         CTGGCTAACAACCAGGTGCGTGCACCCGGTGCCCAGTCCCTGGCTCACGCTCTGGCACAC
XM_005248872.1      CTGGCTAACAACCAGGTGCGTGCACCCGGTGCCCAGTCCCTGGCTCACGCTCTGGCACAC
                    ************************************************************

HsCD00435260        AACACCAACCTCATTTCCCTCAACCTACGTCTCAACTGCATCGAGGATGAGGGTGGCCAG
NM_182539.3         AACACCAACCTCATTTCCCTCAACCTACGTCTCAACTGCATCGAGGATGAGGGTGGCCAG
XM_005248872.1      AACACCAACCTCATTTCCCTCAACCTACGTCTCAACTGCATCGAGGATGAGGGTGGCCAG
                    ************************************************************

HsCD00435260        GCTCTTGCCCATGCCTTGCAGACCAACAAGTGCCTCACCACGCTGCACCTCGGTGGCAAT
NM_182539.3         GCTCTTGCCCATGCCTTGCAGACCAACAAGTGCCTCACCACGCTGCACCTCGGTGGCAAT
XM_005248872.1      GCTCTTGCCCATGCCTTGCAGACCAACAAGTGCCTCACCACGCTGCACCTCGGTGGCAAT
                    ************************************************************

HsCD00435260        GAGCTGTCTGAGCCCACCGCCACACTCCTGTCACAGGTGCTCGCCATCAACACCACACTC
NM_182539.3         GAGCTGTCTGAGCCCACCGCCACACTCCTGTCACAGGTGCTCGCCATCAACACCACACTC
XM_005248872.1      GAGCTGTCTGAGCCCACCGCCACACTCCTGTCACAGGTGCTCGCCATCAACACCACACTC
                    ************************************************************

HsCD00435260        ACCAGCATCAACCTGTCCTGCAACCACATCGGGCTGGACGGTGGGAAGCAGCTCCTGGAA
NM_182539.3         ACCAGCATCAACCTGTCCTGCAACCACATCGGGCTGGACGGTGGGAAGCAGCTCCTGGAA
XM_005248872.1      ACCAGCATCAACCTGTCCTGCAACCACATCGGGCTGGACGGTGGGAAGCAGCTCCTGGAA
                    ************************************************************

HsCD00435260        GGCATGTCAGACAACAAGACCCTCCTGGAATTTGACTTGCGCCTGTCAGATGTGGCCCAG
NM_182539.3         GGCATGTCAGACAACAAGACCCTCCTGGAATTTGACTTGCGCCTGTCAGATGTGGCCCAG
XM_005248872.1      GGCATGTCAGACAACAAGACCCTCCTGGAATTTGACTTGCGCCTGTCAGATGTGGCCCAG
                    ************************************************************

HsCD00435260        GAAAGCGAGTACCTCATTGGCCAGGCCCTCTACGCAAACCGAGAAGCAGCCCGCCAGCGG
NM_182539.3         GAAAGCGAGTACCTCATTGGCCAGGCCCTCTACGCAAACCGAGAAGCAGCCCGCCAGCGG
XM_005248872.1      GAAAGCGAGTACCTCATTGGCCAGGCCCTCTACGCAAACCGAGAAGCAGCCCGCCAGCGG
                    ************************************************************

HsCD00435260        GCCCTGAATCCCAGCCACTTCATGTCAACCATCACTGCCAATGGCCCTGAGAACTCTGTG
NM_182539.3         GCCCTGAATCCCAGCCACTTCATGTCAACCATAACTGCCAATGGCCCTGAGAACTCTGTG
XM_005248872.1      GCCCTGAATCCCAGCCACTTCATGTCAACCATAACTGCCAATGGCCCTGAGAACTCTGTG
                    ********************************.***************************

HsCD00435260        GGA---
NM_182539.3         GGATAA
XM_005248872.1      GGATAA
                    ***   

Clone

Original Clone ID : ccsbBroad304_09824

Organism


TAX_ID : 9606
Species Specific ID: 202500

Genome

Chromosome : 6
Map Location : 6p21.1

Reagents

Labome : TCTE1-antibody