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GPSM3 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  GPSM3
Gene Name:  G-protein signaling modulator 3
Sequence             
Original Clone ID: ccsbBroad304_03912
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00435538

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 481nts         Open reading frame : 1 to 481
Reference Sequence Alignment

ATGGAGGCTGAGAGACCCCAGGAAGAAGAGGATGGTGAGCAGGGCCCCCCTCAGGATGAG
GAAGGCTGGCCCCCTCCAAACTCCACCACTCGGCCTTGGCGATCTGCTCCTCCATCCCCT
CCTCCTCCAGGGACCCGCCACACAGCCCTGGGACCCCGCTCGGCCTCCCTGCTCTCCCTG
CAGACTGAACTCCTTCTGGACCTGGTGGCTGAAGCCCAGTCCCGCCGCCTGGAGGAGCAG
AGGGCCACCTTCTACACCCCCCAAAACCCCTCAAGCCTAGCCCCTGCCCCACTCCGTCCT
CTCGAGGACAGAGAACAGCTTTACAGCACTATCCTCAGTCACCAGTGCCAGCGGATGGAA
GCCCAGCGGTCAGAGCCTCCCCTCCCTCCAGGGGGGCAAGAGCTCCTGGAGTTGCTGCTG
AGAGTTCAGGGTGGGGGTCGAATGGAGGAGCAAAGGTCCCGGCCCCCCACACACACCTGC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GPSM3
Symbol Nomenclature : GPSM3
SYNONYM : AGS4; C6orf9; G18; G18.1a; G18.1b; G18.2; NG1
Designation : G-protein signaling modulator 3 (AGS3-like, C. elegans)|G-protein signalling modulator 3 (AGS3-like, C. elegans)|G-protein-signaling modulator 3|activator of G-protein signaling 4
Full Nomenclature : G-protein signaling modulator 3
GENEID : 63940
Locus Tag : DAAP-218M18.6
GenBank Accession : HQ447974
HGNC : 13945
Vega : OTTHUMG00000031244
Target GenBank: HQ447974

 

Reference Sequence Alignment

  

HsCD00435538        ATGGAGGCTGAGAGACCCCAGGAAGAAGAGGATGGTGAGCAGGGCCCCCCTCAGGATGAG
NM_022107.1         ATGGAGGCTGAGAGACCCCAGGAAGAAGAGGATGGTGAGCAGGGCCCCCCTCAGGATGAG
NM_001276501.1      ATGGAGGCTGAGAGACCCCAGGAAGAAGAGGATGGTGAGCAGGGCCCCCCTCAGGATGAG
                    ************************************************************

HsCD00435538        GAAGGCTGGCCCCCTCCAAACTCCACCACTCGGCCTTGGCGATCTGCTCCTCCATCCCCT
NM_022107.1         GAAGGCTGGCCCCCTCCAAACTCCACCACTCGGCCTTGGCGATCTGCTCCTCCATCCCCT
NM_001276501.1      GAAGGCTGGCCCCCTCCAAACTCCACCACTCGGCCTTGGCGATCTGCTCCTCCATCCCCT
                    ************************************************************

HsCD00435538        CCTCCTCCAGGGACCCGCCACACAGCCCTGGGACCCCGCTCGGCCTCCCTGCTCTCCCTG
NM_022107.1         CCTCCTCCAGGGACCCGCCACACAGCCCTGGGACCCCGCTCGGCCTCCCTGCTCTCCCTG
NM_001276501.1      CCTCCTCCAGGGACCCGCCACACAGCCCTGGGACCCCGCTCGGCCTCCCTGCTCTCCCTG
                    ************************************************************

HsCD00435538        CAGACTGAACTCCTTCTGGACCTGGTGGCTGAAGCCCAGTCCCGCCGCCTGGAGGAGCAG
NM_022107.1         CAGACTGAACTCCTTCTGGACCTGGTGGCTGAAGCCCAGTCCCGCCGCCTGGAGGAGCAG
NM_001276501.1      CAGACTGAACTCCTTCTGGACCTGGTGGCTGAAGCCCAGTCCCGCCGCCTGGAGGAGCAG
                    ************************************************************

HsCD00435538        AGGGCCACCTTCTACACCCCCCAAAACCCCTCAAGCCTAGCCCCTGCCCCACTCCGTCCT
NM_022107.1         AGGGCCACCTTCTACACCCCCCAAAACCCCTCAAGCCTAGCCCCTGCCCCACTCCGTCCT
NM_001276501.1      AGGGCCACCTTCTACACCCCCCAAAACCCCTCAAGCCTAGCCCCTGCCCCACTCCGTCCT
                    ************************************************************

HsCD00435538        CTCGAGGACAGAGAACAGCTTTACAGCACTATCCTCAGTCACCAGTGCCAGCGGATGGAA
NM_022107.1         CTCGAGGACAGAGAACAGCTTTACAGCACTATCCTCAGTCACCAGTGCCAGCGGATGGAA
NM_001276501.1      CTCGAGGACAGAGAACAGCTTTACAGCACTATCCTCAGTCACCAGTGCCAGCGGATGGAA
                    ************************************************************

HsCD00435538        GCCCAGCGGTCAGAGCCTCCCCTCCCTCCAGGGGGGCAAGAGCTCCTGGAGTTGCTGCTG
NM_022107.1         GCCCAGCGGTCAGAGCCTCCCCTCCCTCCAGGGGGGCAAGAGCTCCTGGAGTTGCTGCTG
NM_001276501.1      GCCCAGCGGTCAGAGCCTCCCCTCCCTCCAGGGGGGCAAGAGCTCCTGGAGTTGCTGCTG
                    ************************************************************

HsCD00435538        AGAGTTCAGGGTGGGGGTCGAATGGAGGAGCAAAGGTCCCGGCCCCCCACACACACCTGC
NM_022107.1         AGAGTTCAGGGTGGGGGTCGAATGGAGGAGCAAAGGTCCCGGCCCCCCACACACACCTGC
NM_001276501.1      AGAGTTCAGGGTGGGGGTCGAATGGAGGAGCAAAGGTCCCGGCCCCCCACACACACCTGC
                    ************************************************************

HsCD00435538        ---
NM_022107.1         TGA
NM_001276501.1      TGA
                       

Clone

Original Clone ID : ccsbBroad304_03912

Organism


TAX_ID : 9606
Species Specific ID: 63940

Genome

Chromosome : 6
Map Location : 6p21.3
Ensembl : ENSG00000213654

Reagents

Labome : G18-antibody