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CAPN5 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN5
Gene Name:  calpain 5
Sequence             
Original Clone ID: ccsbBroad304_00187
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00435642

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1921nts         Open reading frame : 1 to 1921
Reference Sequence Alignment

ATGTTCTCGTGTGTGAAGCCCTATGAGGACCAGAACTACTCAGCCCTGAGGCGGGACTGC
CGGCGCAGGAAGGTGCTCTTCGAGGACCCCCTCTTCCCCGCCACTGACGACTCACTCTAC
TATAAGGGCACGCCGGGGCCCGCCGTCAGGTGGAAGCGACCCAAGGGCATCTGCGAGGAC
CCCCGCCTCTTTGTGGATGGCATCAGCTCCCACGACCTGCACCAGGGCCAGGTGGGCAAC
TGCTGGTTTGTGGCAGCCTGCTCGTCACTTGCCTCCCGGGAGTCGCTGTGGCAAAAGGTC
ATCCCAGACTGGAAGGAGCAGGAATGGGACCCCGAAAAGCCCAACGCCTACGCGGGCATC
TTCCACTTCCACTTCTGGCGCTTCGGGGAATGGGTGGACGTGGTCATCGATGACCGGCTG
CCCACAGTCAACAACCAGCTCATCTACTGCCACTCCAACTCCCGCAATGAGTTTTGGTGC
GCCCTAGTGGAGAAGGCCTATGCCAAACTGGCAGGCTGTTACCAGGCCCTGGATGGAGGC
AACACAGCAGACGCACTGGTGGACTTCACGGGTGGTGTTTCTGAGCCCATCGACCTGACC
GAGGGTGACTTTGCCAACGATGAGACTAAGAGGAACCAGCTCTTTGAGCGCATGTTAAAG
GTGCACAGCCGGGGCGGCCTCATCAGTGCCTCCATCAAGGCAGTGACAGCAGCTGACATG
GAGGCCCGCCTGGCGTGCGGCCTGGTAAAGGGCCACGCATACGCCGTCACTGATGTGCGC
AAGGTGCGCCTGGGCCACGGCCTACTGGCCTTCTTCAAGTCAGAGAAGTTGGACATGATC
CGCCTGCGCAACCCCTGGGGCGAGCGGGAGTGGAACGGGCCCTGGAGTGACACCTCGGAG
GAGTGGCAGAAAGTGAGCAAGAGTGAGCGGGAGAAGATGGGTGTGACCGTGCAGGACGAC
GGTGAGTTCTGGATGACCTTCGAGGACGTGTGCCGGTACTTCACGGACATCATCAAGTGC
CGCGTGATCAACACATCCCACCTGAGCATCCACAAGACGTGGGAGGAGGCCCGGCTGCAT
GGCGCCTGGACGCTGCATGAGGACCCGCGACAGAACCGCGGTGGCGGCTGCATCAACCAC
AAGGACACCTTCTTCCAGAACCCACAGTACATCTTCGAAGTCAAGAAGCCAGAAGATGAA
GTCCTGATCTGCATCCAGCAGCGGCCAAAGCGGTCTACGCGCCGGGAGGGCAAGGGTGAG
AACCTGGCCATTGGCTTTGACATCTACAAGGTGGAGGAGAACCGCCAGTACCGCATGCAC
AGCCTGCAGCACAAGGCCGCCAGCTCCATCTACATCAACTCACGCAGCGTCTTCCTGCGC
ACCGACCAGCCCGAGGGCCGCTATGTCATCATCCCCACAACCTTCGAGCCAGGCCACACT
GGCGAGTTCCTGCTCCGAGTCTTCACTGATGTGCCCTCCAACTGCCGGGAGCTGCGCCTG
GATGAGCCCCCACACACCTGCTGGAGCTCCCTCTGTGGCTACCCCCAGCTGGTGACCCAG
GTACATGTCCTGGGAGCTGCTGGCCTCAAGGACTCCCCAACAGGGGCTAACTCTTATGTG
ATCATCAAGTGTGAGGGAGACAAAGTCCGCTCGGCTGTGCAGAAGGGCACCTCCACACCA
GAGTACAATGTGAAAGGCATCTTCTACCGCAAGAAGCTGAGCCAGCCCATCACTGTACAG
GTCTGGAACCACCGAGTGCTGAAGGATGAATTTCTGGGCCAGGTGCACCTAAAGGCTGAC
CCGGACAACCTCCAGGCCCTGCATACCCTCCACCTCCGGGACCGAAATAGCCGGCAGCCC
AGCAACCTGCCAGGCACTGTGGCCGTGCACATTCTCAGCAGCACCTCCCTCATGGCTGTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN5
Symbol Nomenclature : CAPN5
SYNONYM : ADNIV; HTRA3; VRNI; nCL-3
Designation : calpain htra-3|calpain-5|new calpain 3|vitreoretinopathy, neovascular inflammatory
Full Nomenclature : calpain 5
GENEID : 726
GenBank Accession : EU176587
HGNC : 1482
MIM : 602537
Vega : OTTHUMG00000165192
Target GenBank: EU176587

 

Reference Sequence Alignment

  

HsCD00435642      ATGTTCTCGTGTGTGAAGCCCTATGAGGACCAGAACTACTCAGCCCTGAGGCGGGACTGC
NM_004055.4       ATGTTCTCGTGTGTGAAGCCCTATGAGGACCAGAACTACTCAGCCCTGAGGCGGGACTGC
                  ************************************************************

HsCD00435642      CGGCGCAGGAAGGTGCTCTTCGAGGACCCCCTCTTCCCCGCCACTGACGACTCACTCTAC
NM_004055.4       CGGCGCAGGAAGGTGCTCTTCGAGGACCCCCTCTTCCCCGCCACTGACGACTCACTCTAC
                  ************************************************************

HsCD00435642      TATAAGGGCACGCCGGGGCCCGCCGTCAGGTGGAAGCGACCCAAGGGCATCTGCGAGGAC
NM_004055.4       TATAAGGGCACGCCGGGGCCCGCCGTCAGGTGGAAGCGACCCAAGGGCATCTGCGAGGAC
                  ************************************************************

HsCD00435642      CCCCGCCTCTTTGTGGATGGCATCAGCTCCCACGACCTGCACCAGGGCCAGGTGGGCAAC
NM_004055.4       CCCCGCCTCTTTGTGGATGGCATCAGCTCCCACGACCTGCACCAGGGCCAGGTGGGCAAC
                  ************************************************************

HsCD00435642      TGCTGGTTTGTGGCAGCCTGCTCGTCACTTGCCTCCCGGGAGTCGCTGTGGCAAAAGGTC
NM_004055.4       TGCTGGTTTGTGGCAGCCTGCTCGTCACTTGCCTCCCGGGAGTCGCTGTGGCAAAAGGTC
                  ************************************************************

HsCD00435642      ATCCCAGACTGGAAGGAGCAGGAATGGGACCCCGAAAAGCCCAACGCCTACGCGGGCATC
NM_004055.4       ATCCCAGACTGGAAGGAGCAGGAATGGGACCCCGAAAAGCCCAACGCCTACGCGGGCATC
                  ************************************************************

HsCD00435642      TTCCACTTCCACTTCTGGCGCTTCGGGGAATGGGTGGACGTGGTCATCGATGACCGGCTG
NM_004055.4       TTCCACTTCCACTTCTGGCGCTTCGGGGAATGGGTGGACGTGGTCATCGATGACCGGCTG
                  ************************************************************

HsCD00435642      CCCACAGTCAACAACCAGCTCATCTACTGCCACTCCAACTCCCGCAATGAGTTTTGGTGC
NM_004055.4       CCCACAGTCAACAACCAGCTCATCTACTGCCACTCCAACTCCCGCAATGAGTTTTGGTGC
                  ************************************************************

HsCD00435642      GCCCTAGTGGAGAAGGCCTATGCCAAACTGGCAGGCTGTTACCAGGCCCTGGATGGAGGC
NM_004055.4       GCCCTAGTGGAGAAGGCCTATGCCAAACTGGCAGGCTGTTACCAGGCCCTGGATGGAGGC
                  ************************************************************

HsCD00435642      AACACAGCAGACGCACTGGTGGACTTCACGGGTGGTGTTTCTGAGCCCATCGACCTGACC
NM_004055.4       AACACAGCAGACGCACTGGTGGACTTCACGGGTGGTGTTTCTGAGCCCATCGACCTGACC
                  ************************************************************

HsCD00435642      GAGGGTGACTTTGCCAACGATGAGACTAAGAGGAACCAGCTCTTTGAGCGCATGTTAAAG
NM_004055.4       GAGGGTGACTTTGCCAACGATGAGACTAAGAGGAACCAGCTCTTTGAGCGCATGTTAAAG
                  ************************************************************

HsCD00435642      GTGCACAGCCGGGGCGGCCTCATCAGTGCCTCCATCAAGGCAGTGACAGCAGCTGACATG
NM_004055.4       GTGCACAGCCGGGGCGGCCTCATCAGTGCCTCCATCAAGGCAGTGACAGCAGCTGACATG
                  ************************************************************

HsCD00435642      GAGGCCCGCCTGGCGTGCGGCCTGGTAAAGGGCCACGCATACGCCGTCACTGATGTGCGC
NM_004055.4       GAGGCCCGCCTGGCGTGCGGCCTGGTAAAGGGCCACGCATACGCCGTCACTGATGTGCGC
                  ************************************************************

HsCD00435642      AAGGTGCGCCTGGGCCACGGCCTACTGGCCTTCTTCAAGTCAGAGAAGTTGGACATGATC
NM_004055.4       AAGGTGCGCCTGGGCCACGGCCTACTGGCCTTCTTCAAGTCAGAGAAGTTGGACATGATC
                  ************************************************************

HsCD00435642      CGCCTGCGCAACCCCTGGGGCGAGCGGGAGTGGAACGGGCCCTGGAGTGACACCTCGGAG
NM_004055.4       CGCCTGCGCAACCCCTGGGGCGAGCGGGAGTGGAACGGGCCCTGGAGTGACACCTCGGAG
                  ************************************************************

HsCD00435642      GAGTGGCAGAAAGTGAGCAAGAGTGAGCGGGAGAAGATGGGTGTGACCGTGCAGGACGAC
NM_004055.4       GAGTGGCAGAAAGTGAGCAAGAGTGAGCGGGAGAAGATGGGTGTGACCGTGCAGGACGAC
                  ************************************************************

HsCD00435642      GGTGAGTTCTGGATGACCTTCGAGGACGTGTGCCGGTACTTCACGGACATCATCAAGTGC
NM_004055.4       GGTGAGTTCTGGATGACCTTCGAGGACGTGTGCCGGTACTTCACGGACATCATCAAGTGC
                  ************************************************************

HsCD00435642      CGCGTGATCAACACATCCCACCTGAGCATCCACAAGACGTGGGAGGAGGCCCGGCTGCAT
NM_004055.4       CGCGTGATCAACACATCCCACCTGAGCATCCACAAGACGTGGGAGGAGGCCCGGCTGCAT
                  ************************************************************

HsCD00435642      GGCGCCTGGACGCTGCATGAGGACCCGCGACAGAACCGCGGTGGCGGCTGCATCAACCAC
NM_004055.4       GGCGCCTGGACGCTGCATGAGGACCCGCGACAGAACCGCGGTGGCGGCTGCATCAACCAC
                  ************************************************************

HsCD00435642      AAGGACACCTTCTTCCAGAACCCACAGTACATCTTCGAAGTCAAGAAGCCAGAAGATGAA
NM_004055.4       AAGGACACCTTCTTCCAGAACCCACAGTACATCTTCGAAGTCAAGAAGCCAGAAGATGAA
                  ************************************************************

HsCD00435642      GTCCTGATCTGCATCCAGCAGCGGCCAAAGCGGTCTACGCGCCGGGAGGGCAAGGGTGAG
NM_004055.4       GTCCTGATCTGCATCCAGCAGCGGCCAAAGCGGTCTACGCGCCGGGAGGGCAAGGGTGAG
                  ************************************************************

HsCD00435642      AACCTGGCCATTGGCTTTGACATCTACAAGGTGGAGGAGAACCGCCAGTACCGCATGCAC
NM_004055.4       AACCTGGCCATTGGCTTTGACATCTACAAGGTGGAGGAGAACCGCCAGTACCGCATGCAC
                  ************************************************************

HsCD00435642      AGCCTGCAGCACAAGGCCGCCAGCTCCATCTACATCAACTCACGCAGCGTCTTCCTGCGC
NM_004055.4       AGCCTGCAGCACAAGGCCGCCAGCTCCATCTACATCAACTCACGCAGCGTCTTCCTGCGC
                  ************************************************************

HsCD00435642      ACCGACCAGCCCGAGGGCCGCTATGTCATCATCCCCACAACCTTCGAGCCAGGCCACACT
NM_004055.4       ACCGACCAGCCCGAGGGCCGCTATGTCATCATCCCCACAACCTTCGAGCCAGGCCACACT
                  ************************************************************

HsCD00435642      GGCGAGTTCCTGCTCCGAGTCTTCACTGATGTGCCCTCCAACTGCCGGGAGCTGCGCCTG
NM_004055.4       GGCGAGTTCCTGCTCCGAGTCTTCACTGATGTGCCCTCCAACTGCCGGGAGCTGCGCCTG
                  ************************************************************

HsCD00435642      GATGAGCCCCCACACACCTGCTGGAGCTCCCTCTGTGGCTACCCCCAGCTGGTGACCCAG
NM_004055.4       GATGAGCCCCCACACACCTGCTGGAGCTCCCTCTGTGGCTACCCCCAGCTGGTGACCCAG
                  ************************************************************

HsCD00435642      GTACATGTCCTGGGAGCTGCTGGCCTCAAGGACTCCCCAACAGGGGCTAACTCTTATGTG
NM_004055.4       GTACATGTCCTGGGAGCTGCTGGCCTCAAGGACTCCCCAACAGGGGCTAACTCTTATGTG
                  ************************************************************

HsCD00435642      ATCATCAAGTGTGAGGGAGACAAAGTCCGCTCGGCTGTGCAGAAGGGCACCTCCACACCA
NM_004055.4       ATCATCAAGTGTGAGGGAGACAAAGTCCGCTCGGCTGTGCAGAAGGGCACCTCCACACCA
                  ************************************************************

HsCD00435642      GAGTACAATGTGAAAGGCATCTTCTACCGCAAGAAGCTGAGCCAGCCCATCACTGTACAG
NM_004055.4       GAGTACAATGTGAAAGGCATCTTCTACCGCAAGAAGCTGAGCCAGCCCATCACTGTACAG
                  ************************************************************

HsCD00435642      GTCTGGAACCACCGAGTGCTGAAGGATGAATTTCTGGGCCAGGTGCACCTAAAGGCTGAC
NM_004055.4       GTCTGGAACCACCGAGTGCTGAAGGATGAATTTCTGGGCCAGGTGCACCTAAAGGCTGAC
                  ************************************************************

HsCD00435642      CCGGACAACCTCCAGGCCCTGCATACCCTCCACCTCCGGGACCGAAATAGCCGGCAGCCC
NM_004055.4       CCGGACAACCTCCAGGCCCTGCATACCCTCCACCTCCGGGACCGAAATAGCCGGCAGCCC
                  ************************************************************

HsCD00435642      AGCAACCTGCCAGGCACTGTGGCCGTGCACATTCTCAGCAGCACCTCCCTCATGGCTGTC
NM_004055.4       AGCAACCTGCCAGGCACTGTGGCCGTGCACATTCTCAGCAGCACCTCCCTCATGGCTGTC
                  ************************************************************

HsCD00435642      ---
NM_004055.4       TGA
                     

Pathways

Panther : Huntington disease
WikiPathway : Integrin-mediated cell adhesion

Clone

Original Clone ID : ccsbBroad304_00187

Organism


TAX_ID : 9606
Species Specific ID: 726

Genome

Chromosome : 11
Map Location : 11q14
Ensembl : ENSG00000149260

Reagents

Labome : CAPN5-antibody