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ACTR3 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTR3
Gene Name:  ARP3 actin-related protein 3 homolog (yeast)
Sequence             
Original Clone ID: ccsbBroad304_02313
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00435777

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1255nts        
Open reading frame : 1 to 1255
Reference Sequence Alignment

ATGGCGGGACGGCTGCCGGCCTGTGTGGTGGACTGTGGCACGGGGTATACAAAACTAGGA
TATGCTGGAAATACAGAACCACAGTTTATCATCCCTTCCTGTATTGCTATTAAGGAGTCA
GCAAAAGTGGGTGATCAAGCTCAAAGGAGGGTGATGAAAGGTGTTGATGACCTAGACTTC
TTCATTGGTGATGAAGCAATAGAAAAACCTACATATGCAACAAAGTGGCCAATCCGCCAT
GGTATAGTTGAAGATTGGGACTTAATGGAAAGGTTTATGGAGCAAGTGATCTTTAAATAT
TTAAGGGCAGAACCTGAAGACCATTATTTTCTTTTGACTGAACCTCCATTGAATACTCCA
GAAAACAGGGAATATACTGCTGAAATAATGTTTGAGTCCTTCAATGTTCCAGGCTTGTAC
ATTGCTGTGCAGGCTGTTCTTGCCTTAGCTGCATCTTGGACCTCAAGACAAGTAGGAGAA
CGGACGTTGACCGGTACGGTAATAGACAGTGGAGATGGTGTCACTCATGTCATTCCTGTG
GCTGAAGGGTATGTGATTGGCAGCTGTATTAAACACATTCCAATCGCAGGACGAGATATA
ACATATTTTATTCAGCAACTGCTGAGAGACCGAGAAGTAGGAATCCCTCCAGAACAATCC
TTGGAAACTGCTAAGGCAGTAAAGGAGCGCTATAGTTATGTCTGCCCAGATTTAGTAAAA
GAATTTAACAAGTATGATACAGATGGGTCAAAATGGATTAAACAGTATACTGGAATCAAT
GCTATCTCAAAGAAAGAGTTTTCTATCGATGTTGGTTATGAGAGATTTTTGGGACCTGAA
ATCTTTTTTCATCCAGAGTTTGCTAATCCAGACTTTACACAACCTATCTCAGAAGTTGTA
GATGAAGTAATTCAGAATTGTCCTATTGATGTCAGACGTCCTCTCTACAAGAATATTGTC
CTCTCTGGAGGTTCAACCATGTTCAGGGACTTTGGACGTCGCTTGCAAAGAGATTTGAAA
AGAACTGTAGATGCCCGGCTGAAATTAAGTGAGGAATTGAGTGGTGGTAGATTGAAGCCA
AAACCTATTGATGTACAAGTCATTACACACCACATGCAGCGATATGCAGTTTGGTTTGGA
GGATCAATGCTGGCTTCCACGCCTGAGTTCTACCAAGTATGCCACACCAAAAAGGATTAT
GAAGAAATTGGACCTAGCATTTGTCGTCACAATCCAGTGTTTGGAGTCATGTCG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  CMV forward
Reverse:  None
Description: with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: EvNO00877063
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter CMV CMV promoter 14 566
promoter T7 T7 promoter 3690 3672
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker BlastR Blasticidin resistance gene 8753 9148
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTR3
Symbol Nomenclature : ACTR3
SYNONYM : ARP3
Designation : actin-like protein 3|actin-related protein 3
Full Nomenclature : ARP3 actin-related protein 3 homolog (yeast)
GENEID : 10096
HGNC : 170
MIM : 604222
Vega : OTTHUMG00000153497
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00435777      ATGGCGGGACGGCTGCCGGCCTGTGTGGTGGACTGTGGCACGGGGTATACAAAACTAGGA
NM_005721.4       ATGGCGGGACGGCTGCCGGCCTGTGTGGTGGACTGTGGCACGGGGTATACAAAACTAGGA
NM_020445.5       ATGGCAGGCTCCCTGCCTCCCTGCGTGGTGGACTGTGGCACCGGGTATACCAAGCTTGGC
                  *****.**.   *****  **** ***************** ********.**.**:**.

HsCD00435777      TATGCTGGAAATACAGAACCACAGTTTATCATCCCTTCCTGTATTGCTATTAAGGAGTCA
NM_005721.4       TATGCTGGAAATACAGAACCACAGTTTATCATCCCTTCCTGTATTGCTATTAAGGAGTCA
NM_020445.5       TACGCAGGCAACACTGAGCCCCAGTTCATTATTCCTTCATGTATTGCCATCAGAGAGTCA
                  ** **:**.** **:**.**.***** ** ** *****.******** ** *..******

HsCD00435777      GCAAAAGTGGGTGATCAAGCTCAAAGGAGGGTGATGAAAGGTGTTGATGACCTAGACTTC
NM_005721.4       GCAAAAGTGGGTGATCAAGCTCAAAGGAGGGTGATGAAAGGTGTTGATGACCTAGACTTC
NM_020445.5       GCAAAGGTAGTTGACCAAGCTCAAAGGAGAGTGTTGAGGGGAGTTGATGACCTTGACTTT
                  *****.**.* *** **************.***:***..**:***********:***** 

HsCD00435777      TTCATTGGTGATGAAGCAATAGAAAAACCTACATATGCAACAAAGTGGCCAATCCGCCAT
NM_005721.4       TTCATTGGTGATGAAGCAATAGAAAAACCTACATATGCAACAAAGTGGCCAATCCGCCAT
NM_020445.5       TTCATAGGAGATGAAGCCATCGATAAACCTACATATGCTACAAAGTGGCCGATACGACAT
                  *****:**:********.**.**:**************:***********.**.**.***

HsCD00435777      GGTATAGTTGAAGATTGGGACTTAATGGAAAGGTTTATGGAGCAAGTGATCTTTAAATAT
NM_005721.4       GGTATAGTTGAAGATTGGGACTTAATGGAAAGGTTTATGGAGCAAGTGATCTTTAAATAT
NM_020445.5       GGAATCATTGAAGACTGGGATCTTATGGAAAGGTTCATGGAGCAAGTGGTTTTTAAATAT
                  **:**..******* *****  *:*********** ************.* *********

HsCD00435777      TTAAGGGCAGAACCTGAAGACCATTATTTTCTTTTGACTGAACCTCCATTGAATACTCCA
NM_005721.4       TTAAGGGCAGAACCTGAAGACCATTATTTTCTTTTGACTGAACCTCCATTGAATACTCCA
NM_020445.5       CTTCGAGCTGAACCTGAGGACCATTATTTTTTAATGACAGAACCTCCACTCAATACACCA
                   *:.*.**:********.************ *::****:********* * *****:***

HsCD00435777      GAAAACAGGGAATATACTGCTGAAATAATGTTTGAGTCCTTCAATGTTCCAGGCTTGTAC
NM_005721.4       GAAAACAGGGAATATACTGCTGAAATAATGTTTGAGTCCTTCAATGTTCCAGGCTTGTAC
NM_020445.5       GAAAACAGAGAGTATCTTGCAGAAATTATGTTTGAATCATTTAACGTACCAGGACTCTAC
                  ********.**.***. ***:*****:********.**.** ** **:*****. * ***

HsCD00435777      ATTGCTGTGCAGGCTGTTCTTGCCTTAGCTGCATCTTGGACCTCAAGACAAGTAGGAGAA
NM_005721.4       ATTGCTGTGCAGGCTGTTCTTGCCTTAGCTGCATCTTGGACCTCAAGACAAGTAGGAGAA
NM_020445.5       ATTGCAGTTCAGGCAGTGCTGGCCTTGGCGGCATCTTGGACATCTCGACAAGTGGGTGAA
                  *****:** *****:** ** *****.** ***********.**:.*******.**:***

HsCD00435777      CGGACGTTGACCGGTACGGTAATAGACAGTGGAGATGGTGTCACTCATGTCATTCCTGTG
NM_005721.4       CGGACGTTGACCGGTACGGTAATAGACAGTGGAGATGGTGTCACTCATGTCATTCCTGTG
NM_020445.5       CGTACGTTAACGGGGATAGTCATTGACAGCGGAGATGGAGTCACCCATGTTATCCCAGTG
                  ** *****.** ** * .**.**:***** ********:***** ***** ** **:***

HsCD00435777      GCTGAAGGGTATGTGATTGGCAGCTGTATTAAACACATTCCAATCGCAGGACGAGATATA
NM_005721.4       GCTGAAGGGTATGTGATTGGCAGCTGTATTAAACACATTCCAATCGCAGGACGAGATATA
NM_020445.5       GCAGAAGGTTATGTAATTGGAAGCTGCATCAAACACATCCCGATTGCAGGTAGAGATATT
                  **:***** *****.*****.***** ** ******** **.** *****:.*******:

HsCD00435777      ACATATTTTATTCAGCAACTGCTGAGAGACCGAGAAGTAGGAATCCCTCCAGAACAATCC
NM_005721.4       ACATATTTTATTCAGCAACTGCTGAGAGACCGAGAAGTAGGAATCCCTCCAGAACAATCC
NM_020445.5       ACGTATTTCATTCAACAGCTGCTAAGGGAGAGGGAGGTGGGAATCCCTCCTGAGCAGTCA
                  **.***** *****.**.*****.**.** .*.**.**.***********:**.**.**.

HsCD00435777      TTGGAAACTGCTAAGGCAGTAAAGGAGCGCTATAGTTATGTCTGCCCAGATTTAGTAAAA
NM_005721.4       TTGGAAACTGCTAAGGCAGTAAAGGAGCGCTATAGTTATGTCTGCCCAGATTTAGTAAAA
NM_020445.5       CTGGAGACCGCAAAAGCCATTAAGGAGAAATACTGTTACATTTGCCCCGATATAGTCAAG
                   ****.** **:**.**..*:******...** :**** .* *****.***:****.**.

HsCD00435777      GAATTTAACAAGTATGATACAGATGGGTCAAAATGGATTAAACAGTATACTGGAATCAAT
NM_005721.4       GAATTTAACAAGTATGATACAGATGGGTCAAAATGGATTAAACAGTATACTGGAATCAAT
NM_020445.5       GAATTTGCCAAGTATGATGTGGATCCCCGGAAGTGGATCAAACAGTACACGGGTATCAAT
                  ******..**********. .***     .**.***** ******** ** **:******

HsCD00435777      GCTATCTCAAAGAAAGAGTTTTCTATCGATGTTGGTTATGAGAGATTTTTGGGACCTGAA
NM_005721.4       GCTATCTCAAAGAAAGAGTTTTCTATCGATGTTGGTTATGAGAGATTTTTGGGACCTGAA
NM_020445.5       GCGATCAACCAGAAGAAGTTTGTTATAGACGTTGGTTACGAAAGATTCCTGGGACCTGAA
                  ** ***:...****..*****  ***.** ******** **.*****  ***********

HsCD00435777      ATCTTTTTTCATCCAGAGTTTGCTAATCCAGACTTTACACAACCTATCTCAGAAGTTGTA
NM_005721.4       ATCTTTTTTCATCCAGAGTTTGCTAATCCAGACTTTACACAACCTATCTCAGAAGTTGTA
NM_020445.5       ATATTCTTTCACCCGGAGTTTGCCAACCCAGACTTTATGGAGTCCATCTCAGATGTTGTT
                  **.** ***** **.******** ** ********** . *. * ********:*****:

HsCD00435777      GATGAAGTAATTCAGAATTGTCCTATTGATGTCAGACGTCCTCTCTACAAGAATATTGTC
NM_005721.4       GATGAAGTAATTCAGAATTGTCCTATTGATGTCAGACGTCCTCTCTACAAGAATATTGTC
NM_020445.5       GATGAAGTAATACAGAACTGCCCCATCGATGTGCGGCGCCCGCTGTATAAGAATGTCGTA
                  ***********:***** ** ** ** ***** .*.** ** ** ** ******.* **.

HsCD00435777      CTCTCTGGAGGTTCAACCATGTTCAGGGACTTTGGACGTCGCTTGCAAAGAGATTTGAAA
NM_005721.4       CTCTCTGGAGGTTCAACCATGTTCAGGGACTTTGGACGTCGCTTGCAAAGAGATTTGAAA
NM_020445.5       CTCTCAGGAGGCTCCACCATGTTCAGGGATTTCGGACGCCGACTGCAGAGGGATTTGAAG
                  *****:***** **.************** ** ***** **. ****.**.********.

HsCD00435777      AGAACTGTAGATGCCCGGCTGAAATTAAGTGAGGAATTGAGTGGTGGTAGATTGAAGCCA
NM_005721.4       AGAACTGTAGATGCCCGGCTGAAATTAAGTGAGGAATTGAGTGGTGGTAGATTGAAGCCA
NM_020445.5       AGAGTGGTGGATGCTAGGCTGAGGCTCAGCGAGGAGCTCAGCGGCGGGAGGATCAAGCCG
                  ***.  **.***** .******.. *.** *****. * ** ** ** **.:* *****.

HsCD00435777      AAACCTATTGATGTACAAGTCATTACACACCACATGCAGCGATATGCAGTTTGGTTTGGA
NM_005721.4       AAACCTATTGATGTACAAGTCATTACACACCACATGCAGCGATATGCAGTTTGGTTTGGA
NM_020445.5       AAGCCTGTGGAGGTCCAGGTGGTCACGCATCACATGCAGCGCTACGCCGTGTGGTTCGGA
                  **.***.* ** **.**.** .* **.** ***********.** **.** ***** ***

HsCD00435777      GGATCAATGCTGGCTTCCACGCCTGAGTTCTACCAAGTATGCCACACCAAAAAGGATTAT
NM_005721.4       GGATCAATGCTGGCTTCCACGCCTGAGTTCTACCAAGTATGCCACACCAAAAAGGATTAT
NM_020445.5       GGCTCCATGCTGGCCTCGACTCCCGAGTTCTTTCAGGTCTGCCACACCAAGAAGGACTAT
                  **.**.******** ** ** ** *******: **.**.***********.***** ***

HsCD00435777      GAAGAAATTGGACCTAGCATTTGTCGTCACAATCCAGTGTTTGGAGTCATGTCG---
NM_005721.4       GAAGAAATTGGACCTAGCATTTGTCGTCACAATCCAGTGTTTGGAGTCATGTCGTAA
NM_020445.5       GAAGAGTACGGGCCCAGCATCTGCCGCCACAACCCCGTCTTTGGAGTCATGTCCTAG
                  *****.:: **.** ***** ** ** ***** **.** **************    

Pathways

NCI : CDC42 signaling events
NCI : ErbB1 downstream signaling
NCI : PDGFR-beta signaling pathway
NCI : RAC1 signaling pathway
Reactome : Fcgamma receptor (FCGR) dependent phagocytosis
Reactome : Immune System
Reactome : Innate Immune System
Reactome : Regulation of actin dynamics for phagocytic cup formation

Protein

SMART domain : ACTIN : Actin
HPRD : 05024

Clone

Original Clone ID : ccsbBroad304_02313

Organism


TAX_ID : 9606
Species Specific ID: 10096

Genome

Chromosome : 2
Map Location : 2q14.1
Ensembl : ENSG00000115091

Reagents

Labome : ACTR3-antibody