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AQP11 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  AQP11
Gene Name:  aquaporin 11
Sequence             
Original Clone ID: ccsbBroad304_05339
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00435862

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 814nts         Open reading frame : 1 to 814
Reference Sequence Alignment

ATGTCGCCGCTGCTGGGGCTCCGGTCCGAGCTGCAGGACACCTGCACCTCGCTGGGACTG
ATGCTGTCGGTGGTGCTGCTCATGGGGCTGGCCCGCGTAGTCGCCCGGCAGCAGCTGCAC
AGGCCGGTGGCCCACGCCTTCGTCCTGGAGTTTCTAGCCACCTTCCAGCTCTGCTGCTGC
ACCCACGAGCTGCAACTGCTGAGCGAACAGCACCCCGCGCACCCCACCTGGACGCTGACG
CTCGTCTACTTCTTCTCGCTTGTGCATGGCCTGACTCTGGTGGGCACGTCCAGCAACCCG
TGCGGCGTGATGATGCAGATGATGCTGGGGGGCATGTCCCCCGAGACGGGTGCGGTGAGG
CTATTGGCTCAGCTGGTTAGTGCCCTGTGCAGCAGGTACTGCACAAGCGCCTTGTGGAGC
TTGGGTCTGACCCAGTATCACGTCAGCGAGAGGAGCTTCGCTTGCAAGAATCCCATCCGA
GTCGACTTGCTCAAAGCGGTCATCACAGAGGCCGTCTGCTCCTTTCTCTTCCACAGCGCT
CTGCTGCACTTCCAGGAAGTCCGAACCAAGCTTCGTATCCACCTGCTGGCTGCACTCATC
ACCTTTTTGGTCTATGCAGGAGGAAGTCTAACAGGAGCTGTATTTAATCCAGCTTTGGCA
CTTTCGCTACATTTCATGTGTTTTGATGAAGCATTCCCTCAGTTTTTTATAGTATACTGG
CTGGCTCCTTCTTTAGGTATATTGTTGATGATTTTGATGTTCAGCTTTTTCCTTCCATGG
CTGCATAACAACCATACAATTAATAAAAAGGAA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : AQP11
Symbol Nomenclature : AQP11
SYNONYM : AQPX1
Designation : AQP-11|aquaporin-11
Full Nomenclature : aquaporin 11
GENEID : 282679
Locus Tag : PSEC0027
GenBank Accession : HQ448503
HGNC : 19940
MIM : 609914
Vega : OTTHUMG00000165194
Target GenBank: HQ448503

 

Reference Sequence Alignment

  

HsCD00435862      ATGTCGCCGCTGCTGGGGCTCCGGTCCGAGCTGCAGGACACCTGCACCTCGCTGGGACTG
NM_173039.2       ATGTCGCCGCTGCTGGGGCTCCGGTCCGAGCTGCAGGACACCTGCACCTCGCTGGGACTG
                  ************************************************************

HsCD00435862      ATGCTGTCGGTGGTGCTGCTCATGGGGCTGGCCCGCGTAGTCGCCCGGCAGCAGCTGCAC
NM_173039.2       ATGCTGTCGGTGGTGCTGCTCATGGGGCTGGCCCGCGTAGTCGCCCGGCAGCAGCTGCAC
                  ************************************************************

HsCD00435862      AGGCCGGTGGCCCACGCCTTCGTCCTGGAGTTTCTAGCCACCTTCCAGCTCTGCTGCTGC
NM_173039.2       AGGCCGGTGGCCCACGCCTTCGTCCTGGAGTTTCTAGCCACCTTCCAGCTCTGCTGCTGC
                  ************************************************************

HsCD00435862      ACCCACGAGCTGCAACTGCTGAGCGAACAGCACCCCGCGCACCCCACCTGGACGCTGACG
NM_173039.2       ACCCACGAGCTGCAACTGCTGAGCGAACAGCACCCCGCGCACCCCACCTGGACGCTGACG
                  ************************************************************

HsCD00435862      CTCGTCTACTTCTTCTCGCTTGTGCATGGCCTGACTCTGGTGGGCACGTCCAGCAACCCG
NM_173039.2       CTCGTCTACTTCTTCTCGCTTGTGCATGGCCTGACTCTGGTGGGCACGTCCAGCAACCCG
                  ************************************************************

HsCD00435862      TGCGGCGTGATGATGCAGATGATGCTGGGGGGCATGTCCCCCGAGACGGGTGCGGTGAGG
NM_173039.2       TGCGGCGTGATGATGCAGATGATGCTGGGGGGCATGTCCCCCGAGACGGGTGCGGTGAGG
                  ************************************************************

HsCD00435862      CTATTGGCTCAGCTGGTTAGTGCCCTGTGCAGCAGGTACTGCACAAGCGCCTTGTGGAGC
NM_173039.2       CTATTGGCTCAGCTGGTTAGTGCCCTGTGCAGCAGGTACTGCACAAGCGCCTTGTGGAGC
                  ************************************************************

HsCD00435862      TTGGGTCTGACCCAGTATCACGTCAGCGAGAGGAGCTTCGCTTGCAAGAATCCCATCCGA
NM_173039.2       TTGGGTCTGACCCAGTATCACGTCAGCGAGAGGAGCTTCGCTTGCAAGAATCCCATCCGA
                  ************************************************************

HsCD00435862      GTCGACTTGCTCAAAGCGGTCATCACAGAGGCCGTCTGCTCCTTTCTCTTCCACAGCGCT
NM_173039.2       GTCGACTTGCTCAAAGCGGTCATCACAGAGGCCGTCTGCTCCTTTCTCTTCCACAGCGCT
                  ************************************************************

HsCD00435862      CTGCTGCACTTCCAGGAAGTCCGAACCAAGCTTCGTATCCACCTGCTGGCTGCACTCATC
NM_173039.2       CTGCTGCACTTCCAGGAAGTCCGAACCAAGCTTCGTATCCACCTGCTGGCTGCACTCATC
                  ************************************************************

HsCD00435862      ACCTTTTTGGTCTATGCAGGAGGAAGTCTAACAGGAGCTGTATTTAATCCAGCTTTGGCA
NM_173039.2       ACCTTTTTGGTCTATGCAGGAGGAAGTCTAACAGGAGCTGTATTTAATCCAGCTTTGGCA
                  ************************************************************

HsCD00435862      CTTTCGCTACATTTCATGTGTTTTGATGAAGCATTCCCTCAGTTTTTTATAGTATACTGG
NM_173039.2       CTTTCGCTACATTTCATGTGTTTTGATGAAGCATTCCCTCAGTTTTTTATAGTATACTGG
                  ************************************************************

HsCD00435862      CTGGCTCCTTCTTTAGGTATATTGTTGATGATTTTGATGTTCAGCTTTTTCCTTCCATGG
NM_173039.2       CTGGCTCCTTCTTTAGGTATATTGTTGATGATTTTGATGTTCAGCTTTTTCCTTCCATGG
                  ************************************************************

HsCD00435862      CTGCATAACAACCATACAATTAATAAAAAGGAA---
NM_173039.2       CTGCATAACAACCATACAATTAATAAAAAGGAATAA
                  *********************************   

Pathways

Reactome : Aquaporin-mediated transport
Reactome : Passive transport by Aquaporins
Reactome : Transmembrane transport of small molecules

Protein

HPRD : 16503

Clone

Original Clone ID : ccsbBroad304_05339

Organism


TAX_ID : 9606
Species Specific ID: 282679

Genome

Chromosome : 11
Map Location : 11q14.1
Ensembl : ENSG00000178301

Reagents

Labome : AQP11-antibody