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CSTF2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CSTF2
Gene Name:  cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH177775.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00043615

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1734nts         Open reading frame : 1 to 1734
Reference Sequence Alignment

ATGGCGGGTTTGACTGTGAGAGACCCAGCGGTGGATCGTTCTCTACGTTCTGTGTTCGTG
GGGAACATTCCTTATGAAGCTACTGAAGAGCAGTTGAAGGACATCTTTTCTGAGGTTGGA
CCTGTTGTTAGTTTCAGATTGGTATACGATAGAGAGACAGGAAAGCCAAAGGGTTATGGC
TTCTGTGAATACCAAGACCAAGAGACAGCACTTAGTGCCATGCGGAACCTGAATGGGCGC
GAATTCAGTGGGAGAGCACTTCGAGTGGACAATGCTGCCAGTGAAAAGAACAAAGAAGAG
CTGAAGAGCCTTGGCACTGGTGCCCCTGTCATTGAGTCACCTTATGGAGAGACCATCAGT
CCTGAGGATGCCCCTGAGTCCATTAGCAAAGCAGTTGCCAGCCTTCCACCAGAGCAGATG
TTTGAGCTGATGAAACAAATGAAGCTCTGTGTCCAGAATAGTCCCCAGGAGGCACGGAAC
ATGTTACTTCAGAACCCTCAACTGGCTTATGCTTTGCTGCAAGCACAGGTAGTGATGAGA
ATTGTGGATCCGGAAATTGCCCTGAAAATTCTGCATCGCCAGACAAATATCCCAACGCTG
ATTGCAGGCAACCCTCAGCCAGTCCATGGTGCTGGGCCTGGCTCAGGATCCAATGTGTCA
ATGAACCAGCAGAATCCTCAGGCCCCTCAGGCCCAGTCTTTGGGTGGAATGCATGTCAAT
GGCGCACCTCCTCTGATGCAAGCTTCTATGCAGGGTGGAGTTCCAGCACCAGGGCAAATG
CCAGCTGCTGTCACAGGACCTGGCCCTGGTTCCTTAGCTCCTGGAGGAGGAATGCAGGCT
CAGGTTGGAATGCCAGGAAGTGGACCAGTGTCCATGGAACGGGGGCAAGTGCCGATGCAA
GACCCCAGAGCAGCTATGCAGCGGGGATCCTTGCCTGCGAATGTCCCAACCCCTCGAGGC
TTGTTAGGAGATGCTCCGAATGATCCACGGGGAGGCACTTTACTTTCTGTAACTGGAGAG
GTAGAGCCTAGAGGTTACTTGGGACCACCTCATCAGGGTCCACCCATGCACCATGTCCCT
GGCCATGAGAGCCGAGGACCACCCCCACATGAACTGAGGGGAGGGCCATTACCCGAGCCC
AGACCTCTAATGGCAGAACCAAGAGGACCCATGCTAGATCAGAGGGGTCCACCCTTGGAT
GGCAGAGGTGGAAGGGATCCCCGAGGAATAGATGCACGAGGGATGGAGGCCCGAGCCATG
GAGGCAAGAGGGTTAGATGCCAGAGGATTAGAGGCCCGTGCAATGGAGGCCCGTGCGATG
GAAGCTCGTGCAATGGAGGCCCGAGCGATGGAGGCCCGTGCAATGGAAGTCCGAGGGATG
GAGGCCAGAGGCATGGATACCAGAGGCCCAGTGCCTGGCCCCAGAGGACCTATACCTAGT
GGAATGCAGGGTCCCAGTCCAATTAACATGGGGGCGGTTGTCCCCCAGGGATCCAGACAG
GTCCCAGTCATGCAGGGAACAGGAATGCAAGGAGCAAGTATACAGGGTGGAAGCCAGCCT
GGCGGCTTTAGTCCCGGGCAGAACCAAGTCACTCCACAGGATCATGAGAAGGCTGCTTTG
ATTATGCAGGTTCTACAACTGACTGCAGACCAGATTGCCATGTTGCCTCCTGAGCAAAGG
CAGAGTATCCTGATTTTAAAGGAACAAATACAGAAATCCACTGGAGCACCTTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1761
Start on reference sequence 28
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CSTF2
Symbol Nomenclature : CSTF2
SYNONYM : CstF-64
Designation : CF-1 64 kDa subunit|CSTF 64 kDa subunit|betaCstF-64 variant 2|cleavage stimulation factor 64 kDa subunit|cleavage stimulation factor subunit 2
Full Nomenclature : cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
GENEID : 1478
Locus Tag : RP1-146H21.2
HGNC : 2484
MIM : 300907
Vega : OTTHUMG00000022709
Target GenBank: BC017712

 

Reference Sequence Alignment

  

HsCD00043615      ATGGCGGGTTTGACTGTGAGAGACCCAGCGGTGGATCGTTCTCTACGTTCTGTGTTCGTG
NM_001325.2       ATGGCGGGTTTGACTGTGAGAGACCCAGCGGTGGATCGTTCTCTACGTTCTGTGTTCGTG
                  ************************************************************

HsCD00043615      GGGAACATTCCTTATGAAGCTACTGAAGAGCAGTTGAAGGACATCTTTTCTGAGGTTGGA
NM_001325.2       GGGAACATTCCTTATGAAGCTACTGAAGAGCAGTTGAAGGACATCTTTTCTGAGGTTGGA
                  ************************************************************

HsCD00043615      CCTGTTGTTAGTTTCAGATTGGTATACGATAGAGAGACAGGAAAGCCAAAGGGTTATGGC
NM_001325.2       CCTGTTGTTAGTTTCAGATTGGTATACGATAGAGAGACAGGAAAGCCAAAGGGTTATGGC
                  ************************************************************

HsCD00043615      TTCTGTGAATACCAAGACCAAGAGACAGCACTTAGTGCCATGCGGAACCTGAATGGGCGC
NM_001325.2       TTCTGTGAATACCAAGACCAAGAGACAGCACTTAGTGCCATGCGGAACCTGAATGGGCGC
                  ************************************************************

HsCD00043615      GAATTCAGTGGGAGAGCACTTCGAGTGGACAATGCTGCCAGTGAAAAGAACAAAGAAGAG
NM_001325.2       GAATTCAGTGGGAGAGCACTTCGAGTGGACAATGCTGCCAGTGAAAAGAACAAAGAAGAG
                  ************************************************************

HsCD00043615      CTGAAGAGCCTTGGCACTGGTGCCCCTGTCATTGAGTCACCTTATGGAGAGACCATCAGT
NM_001325.2       CTGAAGAGCCTTGGCACTGGTGCCCCTGTCATTGAGTCACCTTATGGAGAGACCATCAGT
                  ************************************************************

HsCD00043615      CCTGAGGATGCCCCTGAGTCCATTAGCAAAGCAGTTGCCAGCCTTCCACCAGAGCAGATG
NM_001325.2       CCTGAGGATGCCCCTGAGTCCATTAGCAAAGCAGTTGCCAGCCTTCCACCAGAGCAGATG
                  ************************************************************

HsCD00043615      TTTGAGCTGATGAAACAAATGAAGCTCTGTGTCCAGAATAGTCCCCAGGAGGCACGGAAC
NM_001325.2       TTTGAGCTGATGAAACAAATGAAGCTCTGTGTCCAGAATAGTCCCCAGGAGGCACGGAAC
                  ************************************************************

HsCD00043615      ATGTTACTTCAGAACCCTCAACTGGCTTATGCTTTGCTGCAAGCACAGGTAGTGATGAGA
NM_001325.2       ATGTTACTTCAGAACCCTCAACTGGCTTATGCTTTGCTGCAAGCACAGGTAGTGATGAGA
                  ************************************************************

HsCD00043615      ATTGTGGATCCGGAAATTGCCCTGAAAATTCTGCATCGCCAGACAAATATCCCAACGCTG
NM_001325.2       ATTGTGGATCCGGAAATTGCCCTGAAAATTCTGCATCGCCAGACAAATATCCCAACGCTG
                  ************************************************************

HsCD00043615      ATTGCAGGCAACCCTCAGCCAGTCCATGGTGCTGGGCCTGGCTCAGGATCCAATGTGTCA
NM_001325.2       ATTGCAGGCAACCCTCAGCCAGTCCATGGTGCTGGGCCTGGCTCAGGATCCAATGTGTCA
                  ************************************************************

HsCD00043615      ATGAACCAGCAGAATCCTCAGGCCCCTCAGGCCCAGTCTTTGGGTGGAATGCATGTCAAT
NM_001325.2       ATGAACCAGCAGAATCCTCAGGCCCCTCAGGCCCAGTCTTTGGGTGGAATGCATGTCAAT
                  ************************************************************

HsCD00043615      GGCGCACCTCCTCTGATGCAAGCTTCTATGCAGGGTGGAGTTCCAGCACCAGGGCAAATG
NM_001325.2       GGCGCACCTCCTCTGATGCAAGCTTCTATGCAGGGTGGAGTTCCAGCACCAGGGCAAATG
                  ************************************************************

HsCD00043615      CCAGCTGCTGTCACAGGACCTGGCCCTGGTTCCTTAGCTCCTGGAGGAGGAATGCAGGCT
NM_001325.2       CCAGCTGCTGTCACAGGACCTGGCCCTGGTTCCTTAGCTCCTGGAGGAGGAATGCAGGCT
                  ************************************************************

HsCD00043615      CAGGTTGGAATGCCAGGAAGTGGACCAGTGTCCATGGAACGGGGGCAAGTGCCGATGCAA
NM_001325.2       CAGGTTGGAATGCCAGGAAGTGGACCAGTGTCCATGGAACGGGGGCAAGTGCCGATGCAA
                  ************************************************************

HsCD00043615      GACCCCAGAGCAGCTATGCAGCGGGGATCCTTGCCTGCGAATGTCCCAACCCCTCGAGGC
NM_001325.2       GACCCCAGAGCAGCTATGCAGCGGGGATCCTTGCCTGCGAATGTCCCAACCCCTCGAGGC
                  ************************************************************

HsCD00043615      TTGTTAGGAGATGCTCCGAATGATCCACGGGGAGGCACTTTACTTTCTGTAACTGGAGAG
NM_001325.2       TTGTTAGGAGATGCTCCGAATGATCCACGGGGAGGCACTTTACTTTCTGTAACTGGAGAG
                  ************************************************************

HsCD00043615      GTAGAGCCTAGAGGTTACTTGGGACCACCTCATCAGGGTCCACCCATGCACCATGTCCCT
NM_001325.2       GTAGAGCCTAGAGGTTACTTGGGACCACCTCATCAGGGTCCACCCATGCACCATGTCCCT
                  ************************************************************

HsCD00043615      GGCCATGAGAGCCGAGGACCACCCCCACATGAACTGAGGGGAGGGCCATTACCCGAGCCC
NM_001325.2       GGCCATGAGAGCCGAGGACCACCCCCACATGAACTGAGGGGAGGGCCATTACCCGAGCCC
                  ************************************************************

HsCD00043615      AGACCTCTAATGGCAGAACCAAGAGGACCCATGCTAGATCAGAGGGGTCCACCCTTGGAT
NM_001325.2       AGACCTCTAATGGCAGAACCAAGAGGACCCATGCTAGATCAGAGGGGTCCACCCTTGGAT
                  ************************************************************

HsCD00043615      GGCAGAGGTGGAAGGGATCCCCGAGGAATAGATGCACGAGGGATGGAGGCCCGAGCCATG
NM_001325.2       GGCAGAGGTGGAAGGGATCCCCGAGGAATAGATGCACGAGGGATGGAGGCCCGAGCCATG
                  ************************************************************

HsCD00043615      GAGGCAAGAGGGTTAGATGCCAGAGGATTAGAGGCCCGTGCAATGGAGGCCCGTGCGATG
NM_001325.2       GAGGCAAGAGGGTTAGATGCCAGAGGATTAGAGGCCCGTGCAATGGAGGCCCGTGCGATG
                  ************************************************************

HsCD00043615      GAAGCTCGTGCAATGGAGGCCCGAGCGATGGAGGCCCGTGCAATGGAAGTCCGAGGGATG
NM_001325.2       GAAGCTCGTGCAATGGAGGCCCGAGCGATGGAGGCCCGTGCAATGGAAGTCCGAGGGATG
                  ************************************************************

HsCD00043615      GAGGCCAGAGGCATGGATACCAGAGGCCCAGTGCCTGGCCCCAGAGGACCTATACCTAGT
NM_001325.2       GAGGCCAGAGGCATGGATACCAGAGGCCCAGTGCCTGGCCCCAGAGGACCTATACCTAGT
                  ************************************************************

HsCD00043615      GGAATGCAGGGTCCCAGTCCAATTAACATGGGGGCGGTTGTCCCCCAGGGATCCAGACAG
NM_001325.2       GGAATGCAGGGTCCCAGTCCAATTAACATGGGGGCGGTTGTCCCCCAGGGATCCAGACAG
                  ************************************************************

HsCD00043615      GTCCCAGTCATGCAGGGAACAGGAATGCAAGGAGCAAGTATACAGGGTGGAAGCCAGCCT
NM_001325.2       GTCCCAGTCATGCAGGGAACAGGAATGCAAGGAGCAAGTATACAGGGTGGAAGCCAGCCT
                  ************************************************************

HsCD00043615      GGCGGCTTTAGTCCCGGGCAGAACCAAGTCACTCCACAGGATCATGAGAAGGCTGCTTTG
NM_001325.2       GGCGGCTTTAGTCCCGGGCAGAACCAAGTCACTCCACAGGATCATGAGAAGGCTGCTTTG
                  ************************************************************

HsCD00043615      ATTATGCAGGTTCTACAACTGACTGCAGACCAGATTGCCATGTTGCCTCCTGAGCAAAGG
NM_001325.2       ATTATGCAGGTTCTACAACTGACTGCAGACCAGATTGCCATGTTGCCTCCTGAGCAAAGG
                  ************************************************************

HsCD00043615      CAGAGTATCCTGATTTTAAAGGAACAAATACAGAAATCCACTGGAGCACCTTAG
NM_001325.2       CAGAGTATCCTGATTTTAAAGGAACAAATACAGAAATCCACTGGAGCACCTTGA
                  ****************************************************..

Pathways

Reactome : Cleavage of Growing Transcript in the Termination Region
Reactome : Gene Expression
Reactome : Post-Elongation Processing of Intron-Containing pre-mRNA
Reactome : Post-Elongation Processing of Intronless pre-mRNA
Reactome : Post-Elongation Processing of the Transcript
Reactome : Processing of Capped Intron-Containing Pre-mRNA
Reactome : Processing of Capped Intronless Pre-mRNA
Reactome : Processing of Intronless Pre-mRNAs
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Termination
Reactome : Transcription
Reactome : mRNA 3'-end processing
Reactome : mRNA Splicing
Reactome : mRNA Splicing - Major Pathway
WikiPathway : mRNA processing

Protein

SMART domain : RRM : RNA recognition motif
UniProt : P33240
HPRD : 02652

Clone

Cloning Information : 177775
HIP Master Clone ID : 152920
Original Clone ID : FLH177775.01X

Organism


TAX_ID : 9606
Species Specific ID: 1478

Genome

Chromosome : X
Map Location : Xq22.1
Ensembl : ENSG00000101811

Reagents

Labome : CSTF2-antibody