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BPIFB3 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  BPIFB3
Gene Name:  BPI fold containing family B, member 3
Sequence             
Original Clone ID: ccsbBroad304_10063
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00436668

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1429nts         Open reading frame : 1 to 1429
Reference Sequence Alignment

ATGCAGCCAGTCATGCTGGCCCTGTGGTCCCTGCTTCTGCTCTGGGGCCTGGCGACTCCA
TGCCAGGAGCTGCTAGAGACGGTGGGCACGCTCGCTCGGATTGACAAGGATGAACTCGGC
AAAGCCATCCAGAACTCACTGGTTGGGGAGCCCATTCTGCAGAATGTGCTGGGATCGGTC
ACAGCTGTGAACCGGGGCCTCTTGGGCTCAGGAGGGCTGCTTGGAGGAGGCGGCTTGCTG
GGCCACGGAGGGGTTTTTGGCGTTGTCGAGGAGCTCTCTGGTCTGAAGATTGAGGAGCTC
ACGCTGCCAAAGGTGTTGCTGAAGCTGCTGCCGGGATTTGGGGTGCAGCTGAGCCTGCAC
ACCAAAGTGGGCATGCATTGCTCTGGCCCCCTTGGTGGCCTTCTGCAGCTGGCTGCGGAG
GTGAACGTGACATCGCGGGTGGCGCTGGCCGTGAGCTCAAGGGGCACACCCATCCTTATC
CTCAAGCGCTGCAGCACGCTCCTGGGCCACATCAGCCTGTTCTCAGGGCTGCTGCCCACA
CCACTCTTTGGGGTCGTGGAACAGATGCTCTTCAAGGTGCTTCCGGGACTGCTGTGCCCC
GTGGTGGACAGTGTGCTGGGTGTGGTGAATGAGCTCCTGGGGGCTGTGCTGGGCCTGGTG
TCCCTTGGGGCTCTTGGGTCCGTGGAATTCTCTCTGGCCACATTGCCTCTCATCTCCAAC
CAGTACATAGAACTGGACATCAACCCTATCGTGAAGAGTGTAGCTGGTGATATCATTGAC
TTCCCCAAGTCCCGTGCCCCAGCCAAGGTGCCCCCCAAGAAGGACCACACATCCCAGGTG
ATGGTGCCACTGTACCTCTTCAACACCATGTTTGGACTCCTGCAGACCAACGGCGCCCTC
GACATGGACATCACCCCTGAGCTGGTTCCCAGCGATGTCCCACTGACAACTACAGACCTG
GCAGCTTTGCTCCCTGAGGCCCTGGGGAAGCTGCCCCTGCACCAGCAACTCCTACTGTTC
CTGCGGGTGAGGGAAGCTCCCACGGTCACACTCCACAACAAGAAGGCCTTGGTCTCCCTC
CCAGCCAACATCCATGTGCTGTTCTATGTCCCTAAGGGGACCCCTGAATCCCTCTTTGAG
CTGAACTCCGTCATGACTGTGCGTGCCCAGCTGGCTCCCTCGGCTACCAAGCTGCACATC
TCCCTGTCCCTGGAACGGCTCAGTGTCAAGGTGGCCTCCTCCTTTACCCATGCCTTTGAC
GGATCGCGTTTAGAAGAATGGCTCAGCCATGTGGTCGGGGCAGTGTATGCACCAAAGCTT
AACGTGGCCCTGGATGTTGGAATTTCCCTGCCTAAGGTTCTTAATATCAATTTTTCCAAT
TCAGTTCTGGAGATCGTAGAGAATGCTGTTGTGCTGACCGTGGCATCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : BPIFB3
Symbol Nomenclature : BPIFB3
SYNONYM : C20orf185; LPLUNC3; RYA3; dJ726C3.4
Designation : BPI fold-containing family B member 3|ligand-binding protein RYA3|long palate, lung and nasal epithelium carcinoma associated 3|long palate, lung and nasal epithelium carcinoma-associated protein 3
Full Nomenclature : BPI fold containing family B, member 3
GENEID : 359710
GenBank Accession : HQ258440
HGNC : 16178
Vega : OTTHUMG00000032234
Target GenBank: HQ258440

 

Reference Sequence Alignment

  

HsCD00436668      ATGCAGCCAGTCATGCTGGCCCTGTGGTCCCTGCTTCTGCTCTGGGGCCTGGCGACTCCA
NM_182658.1       ATGCAGCCAGTCATGCTGGCCCTGTGGTCCCTGCTTCTGCTCTGGGGCCTGGCGACTCCA
                  ************************************************************

HsCD00436668      TGCCAGGAGCTGCTAGAGACGGTGGGCACGCTCGCTCGGATTGACAAGGATGAACTCGGC
NM_182658.1       TGCCAGGAGCTGCTAGAGACGGTGGGCACGCTCGCTCGGATTGACAAGGATGAACTCGGC
                  ************************************************************

HsCD00436668      AAAGCCATCCAGAACTCACTGGTTGGGGAGCCCATTCTGCAGAATGTGCTGGGATCGGTC
NM_182658.1       AAAGCCATCCAGAACTCACTGGTTGGGGAGCCCATTCTGCAGAATGTGCTGGGATCGGTC
                  ************************************************************

HsCD00436668      ACAGCTGTGAACCGGGGCCTCTTGGGCTCAGGAGGGCTGCTTGGAGGAGGCGGCTTGCTG
NM_182658.1       ACAGCTGTGAACCGGGGCCTCTTGGGCTCAGGAGGGCTGCTTGGAGGAGGCGGCTTGCTG
                  ************************************************************

HsCD00436668      GGCCACGGAGGGGTTTTTGGCGTTGTCGAGGAGCTCTCTGGTCTGAAGATTGAGGAGCTC
NM_182658.1       GGCCACGGAGGGGTTTTTGGCGTTGTCGAGGAGCTCTCTGGTCTGAAGATTGAGGAGCTC
                  ************************************************************

HsCD00436668      ACGCTGCCAAAGGTGTTGCTGAAGCTGCTGCCGGGATTTGGGGTGCAGCTGAGCCTGCAC
NM_182658.1       ACGCTGCCAAAGGTGTTGCTGAAGCTGCTGCCGGGATTTGGGGTGCAGCTGAGCCTGCAC
                  ************************************************************

HsCD00436668      ACCAAAGTGGGCATGCATTGCTCTGGCCCCCTTGGTGGCCTTCTGCAGCTGGCTGCGGAG
NM_182658.1       ACCAAAGTGGGCATGCATTGCTCCGGCCCCCTTGGTGGCCTTCTGCAGCTGGCTGCGGAG
                  *********************** ************************************

HsCD00436668      GTGAACGTGACATCGCGGGTGGCGCTGGCCGTGAGCTCAAGGGGCACACCCATCCTTATC
NM_182658.1       GTGAACGTGACATCGCGGGTGGCGCTGGCCGTGAGCTCAAGGGGCACACCCATCCTTATC
                  ************************************************************

HsCD00436668      CTCAAGCGCTGCAGCACGCTCCTGGGCCACATCAGCCTGTTCTCAGGGCTGCTGCCCACA
NM_182658.1       CTCAAGCGCTGCAGCACGCTCCTGGGCCACATCAGCCTGTTCTCAGGGCTGCTGCCCACA
                  ************************************************************

HsCD00436668      CCACTCTTTGGGGTCGTGGAACAGATGCTCTTCAAGGTGCTTCCGGGACTGCTGTGCCCC
NM_182658.1       CCACTCTTTGGGGTCGTGGAACAGATGCTCTTCAAGGTGCTTCCGGGACTGCTGTGCCCC
                  ************************************************************

HsCD00436668      GTGGTGGACAGTGTGCTGGGTGTGGTGAATGAGCTCCTGGGGGCTGTGCTGGGCCTGGTG
NM_182658.1       GTGGTGGACAGTGTGCTGGGTGTGGTGAATGAGCTCCTGGGGGCTGTGCTGGGCCTGGTG
                  ************************************************************

HsCD00436668      TCCCTTGGGGCTCTTGGGTCCGTGGAATTCTCTCTGGCCACATTGCCTCTCATCTCCAAC
NM_182658.1       TCCCTTGGGGCTCTTGGGTCCGTGGAATTCTCTCTGGCCACATTGCCTCTCATCTCCAAC
                  ************************************************************

HsCD00436668      CAGTACATAGAACTGGACATCAACCCTATCGTGAAGAGTGTAGCTGGTGATATCATTGAC
NM_182658.1       CAGTACATAGAACTGGACATCAACCCTATCGTGAAGAGTGTAGCTGGTGATATCATTGAC
                  ************************************************************

HsCD00436668      TTCCCCAAGTCCCGTGCCCCAGCCAAGGTGCCCCCCAAGAAGGACCACACATCCCAGGTG
NM_182658.1       TTCCCCAAGTCCCGTGCCCCAGCCAAGGTGCCCCCCAAGAAGGACCACACATCCCAGGTG
                  ************************************************************

HsCD00436668      ATGGTGCCACTGTACCTCTTCAACACCATGTTTGGACTCCTGCAGACCAACGGCGCCCTC
NM_182658.1       ATGGTGCCACTGTACCTCTTCAACACCACGTTTGGACTCCTGCAGACCAACGGCGCCCTC
                  **************************** *******************************

HsCD00436668      GACATGGACATCACCCCTGAGCTGGTTCCCAGCGATGTCCCACTGACAACTACAGACCTG
NM_182658.1       GACATGGACATCACCCCTGAGCTGGTTCCCAGCGATGTCCCACTGACAACTACAGACCTG
                  ************************************************************

HsCD00436668      GCAGCTTTGCTCCCTGAGGCCCTGGGGAAGCTGCCCCTGCACCAGCAACTCCTACTGTTC
NM_182658.1       GCAGCTTTGCTCCCTGAGGCCCTGGGGAAGCTGCCCCTGCACCAGCAACTCCTACTGTTC
                  ************************************************************

HsCD00436668      CTGCGGGTGAGGGAAGCTCCCACGGTCACACTCCACAACAAGAAGGCCTTGGTCTCCCTC
NM_182658.1       CTGCGGGTGAGGGAAGCTCCCACGGTCACACTCCACAACAAGAAGGCCTTGGTCTCCCTC
                  ************************************************************

HsCD00436668      CCAGCCAACATCCATGTGCTGTTCTATGTCCCTAAGGGGACCCCTGAATCCCTCTTTGAG
NM_182658.1       CCAGCCAACATCCATGTGCTGTTCTATGTCCCTAAGGGGACCCCTGAATCCCTCTTTGAG
                  ************************************************************

HsCD00436668      CTGAACTCCGTCATGACTGTGCGTGCCCAGCTGGCTCCCTCGGCTACCAAGCTGCACATC
NM_182658.1       CTGAACTCCGTCATGACTGTGCGTGCCCAGCTGGCTCCCTCGGCTACCAAGCTGCACATC
                  ************************************************************

HsCD00436668      TCCCTGTCCCTGGAACGGCTCAGTGTCAAGGTGGCCTCCTCCTTTACCCATGCCTTTGAC
NM_182658.1       TCCCTGTCCCTGGAACGGCTCAGTGTCAAGGTGGCCTCCTCCTTTACCCATGCCTTTGAC
                  ************************************************************

HsCD00436668      GGATCGCGTTTAGAAGAATGGCTCAGCCATGTGGTCGGGGCAGTGTATGCACCAAAGCTT
NM_182658.1       GGATCGCGTTTAGAAGAATGGCTCAGCCATGTGGTCGGGGCAGTGTATGCACCAAAGCTT
                  ************************************************************

HsCD00436668      AACGTGGCCCTGGATGTTGGAATTTCCCTGCCTAAGGTTCTTAATATCAATTTTTCCAAT
NM_182658.1       AACGTGGCCCTGGATGTTGGAATTCCCCTGCCTAAGGTTCTTAATATCAATTTTTCCAAT
                  ************************ ***********************************

HsCD00436668      TCAGTTCTGGAGATCGTAGAGAATGCTGTTGTGCTGACCGTGGCATCC---
NM_182658.1       TCAGTTCTGGAGATCGTAGAGAATGCTGTTGTGCTGACCGTGGCATCCTGA
                  ************************************************   

Clone

Original Clone ID : ccsbBroad304_10063

Organism


TAX_ID : 9606
Species Specific ID: 359710

Genome

Chromosome : 20
Map Location : 20q11.21
Ensembl : ENSG00000186190

Reagents

Labome : BPIFB3-antibody