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GABRR1 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  GABRR1
Gene Name:  gamma-aminobutyric acid (GABA) A receptor, rho 1
Sequence             
Original Clone ID: ccsbBroad304_10834
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00436671

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1423nts         Open reading frame : 1 to 1423
Reference Sequence Alignment

ATGAGATTTGGCATCTTTCTTTTGTGGTGGGGATGGGTTTTGGCCACTGAAAGCAGAATG
CACTGGCCCGGAAGAGAAGTCCACGAGATGTCTAAGAAAGGCAGTAGGCCCCAAAGACAA
AGACGAGAAGTACATGAAGATGCCCACAAGCAAGTCAGCCCAATTCTGAGACGAAGTCCT
GACATCACCAAATCGCCTCTGACAAAGTCAGAACAGCTTCTGAGGATAGATGACCATGAT
TTCAGCATGAGGCCTGGCTTTGGAGGCCCTGCCATTCCTGTTGGTGTGGATGTGCAGGTG
GAGAGTTTGGATAGCATCTCAGAGGTTGACATGGACTTTACGATGACCCTCTACCTGAGG
CACTACTGGAAGGACGAGAGGCTGTCTTTTCCAAGCACCAACAACCTCAGCATGACGTTT
GATGGCCGGCTGGTCAAGAAGATCTGGGTCCCTGACATGTTTTTCGTGCACTCCAAACGC
TCCTTCATCCACGACACCACCACAGACAACGTCATGTTGCGGGTCCAGCCTGATGGGAAA
GTGCTCTATAGTCTCAGGGTTACAGTAACTGCAATGTGCAACATGGACTTCAGCCGATTT
CCCTTGGACACACAAACGTGCTCTCTTGAAATTGAAAGCTATGCCTATACAGAAGATGAC
CTCATGCTGTACTGGAAAAAGGGCAATGACTCCTTAAAGACAGATGAACGGATCTCACTC
TCCCAGTTCCTCATTCAGGAATTCCACACCACCACCAAACTGGCTTTCTACAGCAGCACA
GGCTGGTACAACCGTCTCTACATTAATTTCACGTTGCGTCGCCACATCTTCTTCTTCTTG
CTCCAAACTTATTTCCCCGCTACCCTGATGGTCATGCTGTCCTGGGTGTCCTTCTGGATC
GACCGCAGAGCCGTGCCTGCCAGAGTCCCCTTAGGTATCACAACGGTGCTGACCATGTCC
ACCATCATCACGGGCGTGAATGCCTCCATGCCGCGCGTCTCCTACATCAAGGCCGTGGAC
ATCTACCTCTGGGTCAGCTTTGTGTTCGTGTTCCTCTCGGTGCTGGAGTATGCGGCCGTC
AACTACCTGACCACTGTGCAGGAGAGGAAGGAACAGAAGCTGCGGGAGAAGCTTCCCTGC
ACCAGCGGATTACCTCCGCCCCGCACTGCAATGCTGGACGGCAACTACAGTGATGGGGAG
GTGAATGACCTGGACAACTACATGCCAGAGAATGGAGAGAAGCCCGACAGGATGATGGTG
CAGCTGACCCTGGCCTCAGAGAGGAGCTCCCCACAGAGGAAAAGTCAGAGAAGCAGCTAT
GTGAGCATGAGAATCGACACCCACGCCATTGATAAATACTCCAGGATCATCTTTCCAGCA
GCATACATTTTATTCAATTTAATATACTGGTCTATTTTCTCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GABRR1
Symbol Nomenclature : GABRR1
Designation : GABA(A) receptor subunit rho-1|GABA(A) receptor, rho 1|GABA(C) receptor|bA135P14.1 (gamma-aminobutyric acid (GABA) receptor, rho 1)|gamma-aminobutyric acid (GABA) A receptor, rho-1|gamma-aminobutyric acid (GABA) receptor, rho 1|gamma-aminobutyric acid receptor subunit rho-1
Full Nomenclature : gamma-aminobutyric acid (GABA) A receptor, rho 1
GENEID : 2569
Locus Tag : RP11-63L7.1
HGNC : 4090
MIM : 137161
Vega : OTTHUMG00000015195
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00436671        ------------------ATGAGATTTGGCATCTTTCTTTTGTGGTGGGGATGGGTTTTG
NM_002042.4         ATGTTGGCTGTCCCAAATATGAGATTTGGCATCTTTCTTTTGTGGTGGGGATGGGTTTTG
NM_001256703.1      ATGTTGGCTGTCCCAAATATGAGATTTGGCATCTTTCTTTTGTGGTGGGGATGGGTTTTG
                                      ******************************************

HsCD00436671        GCCACTGAAAGCAGAATGCACTGGCCCGGAAGAGAAGTCCACGAGATGTCTAAGAAAGGC
NM_002042.4         GCCACTGAAAGCAGAATGCACTGGCCCGGAAGAGAAGTCCACGAGATGTCTAAGAAAG--
NM_001256703.1      GCCACTGAAAGCAGAATGCACTGGCCCGGAAGAGAAGTCCACGAGATGTCTAAGAAAG--
                    **********************************************************  

HsCD00436671        AGTAGGCCCCAAAGACAAAGACGAGAAGTACATGAAGATGCCCACAAGCAAGTCAGCCCA
NM_002042.4         -GCAGGCCCCAAAGACAAAGACGAGAAGTACATGAAGATGCCCACAAGCAAGTCAGCCCA
NM_001256703.1      -GCAG---------------------------------------------------CCCA
                     * **                                                   ****

HsCD00436671        ATTCTGAGACGAAGTCCTGACATCACCAAATCGCCTCTGACAAAGTCAGAACAGCTTCTG
NM_002042.4         ATTCTGAGACGAAGTCCTGACATCACCAAATCGCCTCTGACAAAGTCAGAACAGCTTCTG
NM_001256703.1      ATTCTGAGACGAAGTCCTGACATCACCAAATCGCCTCTGACAAAGTCAGAACAGCTTCTG
                    ************************************************************

HsCD00436671        AGGATAGATGACCATGATTTCAGCATGAGGCCTGGCTTTGGAGGCCCTGCCATTCCTGTT
NM_002042.4         AGGATAGATGACCATGATTTCAGCATGAGGCCTGGCTTTGGAGGCCCTGCCATTCCTGTT
NM_001256703.1      AGGATAGATGACCATGATTTCAGCATGAGGCCTGGCTTTGGAGGCCCTGCCATTCCTGTT
                    ************************************************************

HsCD00436671        GGTGTGGATGTGCAGGTGGAGAGTTTGGATAGCATCTCAGAGGTTGACATGGACTTTACG
NM_002042.4         GGTGTGGATGTGCAGGTGGAGAGTTTGGATAGCATCTCAGAGGTTGACATGGACTTTACG
NM_001256703.1      GGTGTGGATGTGCAGGTGGAGAGTTTGGATAGCATCTCAGAGGTTGACATGGACTTTACG
                    ************************************************************

HsCD00436671        ATGACCCTCTACCTGAGGCACTACTGGAAGGACGAGAGGCTGTCTTTTCCAAGCACCAAC
NM_002042.4         ATGACCCTCTACCTGAGGCACTACTGGAAGGACGAGAGGCTGTCTTTTCCAAGCACCAAC
NM_001256703.1      ATGACCCTCTACCTGAGGCACTACTGGAAGGACGAGAGGCTGTCTTTTCCAAGCACCAAC
                    ************************************************************

HsCD00436671        AACCTCAGCATGACGTTTGATGGCCGGCTGGTCAAGAAGATCTGGGTCCCTGACATGTTT
NM_002042.4         AACCTCAGCATGACGTTTGACGGCCGGCTGGTCAAGAAGATCTGGGTCCCTGACATGTTT
NM_001256703.1      AACCTCAGCATGACGTTTGACGGCCGGCTGGTCAAGAAGATCTGGGTCCCTGACATGTTT
                    ******************** ***************************************

HsCD00436671        TTCGTGCACTCCAAACGCTCCTTCATCCACGACACCACCACAGACAACGTCATGTTGCGG
NM_002042.4         TTCGTGCACTCCAAACGCTCCTTCATCCACGACACCACCACAGACAACGTCATGTTGCGG
NM_001256703.1      TTCGTGCACTCCAAACGCTCCTTCATCCACGACACCACCACAGACAACGTCATGTTGCGG
                    ************************************************************

HsCD00436671        GTCCAGCCTGATGGGAAAGTGCTCTATAGTCTCAGGGTTACAGTAACTGCAATGTGCAAC
NM_002042.4         GTCCAGCCTGATGGGAAAGTGCTCTATAGTCTCAGGGTTACAGTAACTGCAATGTGCAAC
NM_001256703.1      GTCCAGCCTGATGGGAAAGTGCTCTATAGTCTCAGGGTTACAGTAACTGCAATGTGCAAC
                    ************************************************************

HsCD00436671        ATGGACTTCAGCCGATTTCCCTTGGACACACAAACGTGCTCTCTTGAAATTGAAAGCTAT
NM_002042.4         ATGGACTTCAGCCGATTTCCCTTGGACACACAAACGTGCTCTCTTGAAATTGAAAGCTAT
NM_001256703.1      ATGGACTTCAGCCGATTTCCCTTGGACACACAAACGTGCTCTCTTGAAATTGAAAGCTAT
                    ************************************************************

HsCD00436671        GCCTATACAGAAGATGACCTCATGCTGTACTGGAAAAAGGGCAATGACTCCTTAAAGACA
NM_002042.4         GCCTATACAGAAGATGACCTCATGCTGTACTGGAAAAAGGGCAATGACTCCTTAAAGACA
NM_001256703.1      GCCTATACAGAAGATGACCTCATGCTGTACTGGAAAAAGGGCAATGACTCCTTAAAGACA
                    ************************************************************

HsCD00436671        GATGAACGGATCTCACTCTCCCAGTTCCTCATTCAGGAATTCCACACCACCACCAAACTG
NM_002042.4         GATGAACGGATCTCACTCTCCCAGTTCCTCATTCAGGAATTCCACACCACCACCAAACTG
NM_001256703.1      GATGAACGGATCTCACTCTCCCAGTTCCTCATTCAGGAATTCCACACCACCACCAAACTG
                    ************************************************************

HsCD00436671        GCTTTCTACAGCAGCACAGGCTGGTACAACCGTCTCTACATTAATTTCACGTTGCGTCGC
NM_002042.4         GCTTTCTACAGCAGCACAGGCTGGTACAACCGTCTCTACATTAATTTCACGTTGCGTCGC
NM_001256703.1      GCTTTCTACAGCAGCACAGGCTGGTACAACCGTCTCTACATTAATTTCACGTTGCGTCGC
                    ************************************************************

HsCD00436671        CACATCTTCTTCTTCTTGCTCCAAACTTATTTCCCCGCTACCCTGATGGTCATGCTGTCC
NM_002042.4         CACATCTTCTTCTTCTTGCTCCAAACTTATTTCCCCGCTACCCTGATGGTCATGCTGTCC
NM_001256703.1      CACATCTTCTTCTTCTTGCTCCAAACTTATTTCCCCGCTACCCTGATGGTCATGCTGTCC
                    ************************************************************

HsCD00436671        TGGGTGTCCTTCTGGATCGACCGCAGAGCCGTGCCTGCCAGAGTCCCCTTAGGTATCACA
NM_002042.4         TGGGTGTCCTTCTGGATCGACCGCAGAGCCGTGCCTGCCAGAGTCCCCTTAGGTATCACA
NM_001256703.1      TGGGTGTCCTTCTGGATCGACCGCAGAGCCGTGCCTGCCAGAGTCCCCTTAGGTATCACA
                    ************************************************************

HsCD00436671        ACGGTGCTGACCATGTCCACCATCATCACGGGCGTGAATGCCTCCATGCCGCGCGTCTCC
NM_002042.4         ACGGTGCTGACCATGTCCACCATCATCACGGGCGTGAATGCCTCCATGCCGCGCGTCTCC
NM_001256703.1      ACGGTGCTGACCATGTCCACCATCATCACGGGCGTGAATGCCTCCATGCCGCGCGTCTCC
                    ************************************************************

HsCD00436671        TACATCAAGGCCGTGGACATCTACCTCTGGGTCAGCTTTGTGTTCGTGTTCCTCTCGGTG
NM_002042.4         TACATCAAGGCCGTGGACATCTACCTCTGGGTCAGCTTTGTGTTCGTGTTCCTCTCGGTG
NM_001256703.1      TACATCAAGGCCGTGGACATCTACCTCTGGGTCAGCTTTGTGTTCGTGTTCCTCTCGGTG
                    ************************************************************

HsCD00436671        CTGGAGTATGCGGCCGTCAACTACCTGACCACTGTGCAGGAGAGGAAGGAACAGAAGCTG
NM_002042.4         CTGGAGTATGCGGCCGTCAACTACCTGACCACTGTGCAGGAGAGGAAGGAACAGAAGCTG
NM_001256703.1      CTGGAGTATGCGGCCGTCAACTACCTGACCACTGTGCAGGAGAGGAAGGAACAGAAGCTG
                    ************************************************************

HsCD00436671        CGGGAGAAGCTTCCCTGCACCAGCGGATTACCTCCGCCCCGCACTGCAATGCTGGACGGC
NM_002042.4         CGGGAGAAGCTTCCCTGCACCAGCGGATTACCTCCGCCCCGCACTGCGATGCTGGACGGC
NM_001256703.1      CGGGAGAAGCTTCCCTGCACCAGCGGATTACCTCCGCCCCGCACTGCGATGCTGGACGGC
                    ***********************************************.************

HsCD00436671        AACTACAGTGATGGGGAGGTGAATGACCTGGACAACTACATGCCAGAGAATGGAGAGAAG
NM_002042.4         AACTACAGTGATGGGGAGGTGAATGACCTGGACAACTACATGCCAGAGAATGGAGAGAAG
NM_001256703.1      AACTACAGTGATGGGGAGGTGAATGACCTGGACAACTACATGCCAGAGAATGGAGAGAAG
                    ************************************************************

HsCD00436671        CCCGACAGGATGATGGTGCAGCTGACCCTGGCCTCAGAGAGGAGCTCCCCACAGAGGAAA
NM_002042.4         CCCGACAGGATGATGGTGCAGCTGACCCTGGCCTCAGAGAGGAGCTCCCCACAGAGGAAA
NM_001256703.1      CCCGACAGGATGATGGTGCAGCTGACCCTGGCCTCAGAGAGGAGCTCCCCACAGAGGAAA
                    ************************************************************

HsCD00436671        AGTCAGAGAAGCAGCTATGTGAGCATGAGAATCGACACCCACGCCATTGATAAATACTCC
NM_002042.4         AGTCAGAGAAGCAGCTATGTGAGCATGAGAATCGACACCCACGCCATTGATAAATACTCC
NM_001256703.1      AGTCAGAGAAGCAGCTATGTGAGCATGAGAATCGACACCCACGCCATTGATAAATACTCC
                    ************************************************************

HsCD00436671        AGGATCATCTTTCCAGCAGCATACATTTTATTCAATTTAATATACTGGTCTATTTTCTCC
NM_002042.4         AGGATCATCTTTCCAGCAGCATACATTTTATTCAATTTAATATACTGGTCTATTTTCTCC
NM_001256703.1      AGGATCATCTTTCCAGCAGCATACATTTTATTCAATTTAATATACTGGTCTATTTTCTCC
                    ************************************************************

HsCD00436671        ---
NM_002042.4         TAG
NM_001256703.1      TAG
                       

Pathways

Reactome : GABA A (rho) receptor activation
Reactome : GABA receptor activation
Reactome : Ion channel transport
Reactome : Ligand-gated ion channel transport
Reactome : Neuronal System
Reactome : Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
Reactome : Transmembrane transport of small molecules
Reactome : Transmission across Chemical Synapses

Protein

HPRD : 15921

Clone

Original Clone ID : ccsbBroad304_10834

Organism


TAX_ID : 9606
Species Specific ID: 2569

Genome

Chromosome : 6
Map Location : 6q15
Ensembl : ENSG00000146276

Reagents

Labome : GABRR1-antibody