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CYP3A43 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CYP3A43
Gene Name:  cytochrome P450, family 3, subfamily A, polypeptide 43
Sequence             
Original Clone ID: ccsbBroad304_12497
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00436736

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1180nts         Open reading frame : 1 to 1180
Reference Sequence Alignment

ATGGATCTCATTCCAAACTTTGCCATGGAAACATGGGTTCTTGTGGCTACCAGCCTGGTA
CTCCTCTATATTTATGGGACCCATTCACATAAACTTTTTAAGAAGCTGGGAATTCCTGGG
CCAACCCCTCTGCCTTTTCTGGGAACTATTTTGTTCTACCTTAGGAGGTCTCTGAATAAG
ATTCCCAGCTGGGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAAGCA
GGAGGATCACCTAAGGTCAGGAGTTCGAGACCAGCTTTGCCAACATGGGTCTTTGGAATT
TTGACAGAGAATGTAATGAAAAATACGGAGAAATGTGGGGCACTCTTTCCATTTCTTACC
CCAGTTTTTGAAGCCCTAAATATCGGTTTGTTTCCAAAAGATGTTACCCATTTTTTAAAA
AATTCCATTGAAAGGATGAAAGAAAGTCGCCTCAAAGATAAACAAAAGCATCGAGTAGAT
TTCTTTCAACAGATGATCGACTCCCAGAATTCCAAAGAAACAAAGTCCCATAAAGCTCTG
TCTGATCTGGAGCTTGTGGCCCAGTCAATTATCATCATTTTTGCTGCCTATGACACAACT
AGCACCACTCTCCCCTTCATTATGTATGAACTGGCCACTCACCCTGATGTCCAGCAGAAA
CTGCAGGAGGAGATTGACGCAGTTTTACCCAATAAGGCACCTGTCACCTACGATGCCCTG
GTACAGATGGAGTACCTTGACATGGTGGTGAATGAAACGCTCAGATTATTCCCAGTTGTT
AGTAGAGTTACGAGAGTCTGCAAGAAAGATATTGAAATCAATGGAGTGTTCATTCCCAAA
GGGTTAGCAGTGATGGTTCCAATCTATGCTCTTCACCATGACCCAAAGTACTGGACAGAG
CCTGAGAAGTTCTGCCCTGAAAGGTTCAGTAAGAAGAACAAGGACAGCATAGATCTTTAC
AGATACATACCTTTTGGAGCTGGACCCCGAAACTGCATTGGCATGAGGTTTGCTCTCACA
AACATAAAACTTGCTGTCATTAGAGCACTGCAGAACTTCTCCTTCAAACCTTGTAAAGAG
ACTCAGATCCCACTGAAATTAGACAATCTACCAATTCTTCAACCAGAAAAACCTATTGTT
CTAAAAGTGCACTTAAGAGATGGGATTACAAGTGGACCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CYP3A43
Symbol Nomenclature : CYP3A43
Designation : cytochrome P450 3A43|cytochrome P450, subfamily IIIA, polypeptide 43
Full Nomenclature : cytochrome P450, family 3, subfamily A, polypeptide 43
GENEID : 64816
HGNC : 17450
MIM : 606534
Vega : OTTHUMG00000156498
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00436736        ATGGATCTCATTCCAAACTTTGCCATGGAAACATGGGTTCTTGTGGCTACCAGCCTGGTA
NM_022820.4         ATGGATCTCATTCCAAACTTTGCCATGGAAACATGGGTTCTTGTGGCTACCAGCCTGGTA
NM_001278921.1      ATGGATCTCATTCCAAACTTTGCCATGGAAACATGGGTTCTTGTGGCTACCAGCCTGGTA
NM_057095.2         ATGGATCTCATTCCAAACTTTGCCATGGAAACATGGGTTCTTGTGGCTACCAGCCTGGTA
                    ************************************************************

HsCD00436736        CTCCTCTATATTTATGGGACCCATTCACATAAACTTTTTAAGAAGCTGGGAATTCCTGGG
NM_022820.4         CTCCTCTATATTTATGGGACCCATTCACATAAACTTTTTAAGAAGCTGGGAATTCCTGGG
NM_001278921.1      CTCCTCTATATTTATGGGACCCATTCACATAAACTTTTTAAGAAGCTGGGAATTCCTGGG
NM_057095.2         CTCCTCTATATTTATGGGACCCATTCACATAAACTTTTTAAGAAGCTGGGAATTCCTGGG
                    ************************************************************

HsCD00436736        CCAACCCCTCTGCCTTTTCTGGGAACTATTTTGTTCTACCTTAGGAGGTCTCTGAATAAG
NM_022820.4         CCAACCCCTCTGCCTTTTCTGGGAACTATTTTGTTCTACCTTAGGGGTCTTTGGAATTTT
NM_001278921.1      CCAACCCCTCTGCCTTTTCTGGGAACTATTTTGTTCTACCTTAGGAGGTCTCTGAATAAG
NM_057095.2         CCAACCCCTCTGCCTTTTCTGGGAACTATTTTGTTCTACCTTAGGGGTCTTTGGAATTTT
                    *********************************************.*   *  ****:: 

HsCD00436736        ------------------------------------------------------------
NM_022820.4         GACAGAGAATGTAATGAAAAATACGGAGAAATGTGGGGGCTGTATGAGGGGCAACAGCCC
NM_001278921.1      ------------------------------------------------------------
NM_057095.2         GACAGAGAATGTAATGAAAAATACGGAGAAATGTGGGGGCTGTATGAGGGGCAACAGCCC
                                                                                

HsCD00436736        ------------------------------------------------------------
NM_022820.4         ATGCTGGTCATCATGGATCCCGACATGATCAAAACAGTGTTAGTGAAAGAATGTTACTCT
NM_001278921.1      ------------------------------------------------------------
NM_057095.2         ATGCTGGTCATCATGGATCCCGACATGATCAAAACAGTGTTAGTGAAAGAATGTTACTCT
                                                                                

HsCD00436736        ------------------------------------------------------------
NM_022820.4         GTCTTCACAAACCAGATGCCTTTAGGTCCAATGGGATTTCTGAAAAGTGCCTTAAGTTTT
NM_001278921.1      ------------------------------------------------------------
NM_057095.2         GTCTTCACAAACCAGATGCCTTTAGGTCCAATGGGATTTCTGAAAAGTGCCTTAAGTTTT
                                                                                

HsCD00436736        ------------------------------------------------------------
NM_022820.4         GCTGAAGATGAAGAATGGAAGAGAATACGAACATTGCTATCTCCAGCTTTCACCAGTGTA
NM_001278921.1      ------------------------------------------------------------
NM_057095.2         GCTGAAGATGAAGAATGGAAGAGAATACGAACATTGCTATCTCCAGCTTTCACCAGTGTA
                                                                                

HsCD00436736        -------------------------------------------------ATTCCCAGCTG
NM_022820.4         AAATTCAAGGAAATGGTCCCCATCATTTCCCAATGTGGAGATATGTTGGTGAGAAGCCTG
NM_001278921.1      -------------------------------------------------ATTCCCAGCTG
NM_057095.2         AAATTCAAGGAAATGGTCCCCATCATTTCCCAATGTGGAGATATGTTGGTGAGAAGCCTG
                                                                     : : ... ***

HsCD00436736        GGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAAGCAGGAGGATCACC
NM_022820.4         AGGCAGGAAGCAGAGAACAGCAAGTCCATCAACTTGAAAGATTTCTTTGGGGCCTACACC
NM_001278921.1      GGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAAGCAGGAGGATCACC
NM_057095.2         AGGCAGGAAGCAGAGAACAGCAAGTCCATCAACTTGAAAGATTTCTTTGGGGCCTACACC
                    .*  :**:.**: * ..*:* **  *** **. ***..**. * .:  .**.  ::****

HsCD00436736        TAAGGTCAGGAGTTCGAGACCAGCTTTG--------------------------------
NM_022820.4         ATGGATGTAATCACTGGCACATTATTTGGAGTGAACTTGGATTCTCTCAACAATCCACAA
NM_001278921.1      TAAGGTCAGGAGTTCGAGACCAGCTTTG--------------------------------
NM_057095.2         ATGGATGTAATCACTGGCACATTATTTGGAGTGAACTTGGATTCTCTCAACAATCCACAA
                    ::.*.* :..: :  *. **.: .****                                

HsCD00436736        ---------CCAACATGGGTCTTTGGAATTTTGACAGAGAATGTAATGAAAAATACGGAG
NM_022820.4         GATCCCTTTCTGAAAAATATGAAGAAGCTTTTAAAATTGGATTTTTTGGATCCCTTTTTA
NM_001278921.1      ---------CCAACATGGGTCTTTGGAATTTTGACAGAGAATGTAATGAAAAATACGGAG
NM_057095.2         GATCCCTTTCTGAAAAATATGAAGAAGCTTTTAAAATTGGATTTTTTGGATCCCTTTTTA
                             * .*.*:. .* :: ....****.*.* :*.** *::**.*:.. :   :.

HsCD00436736        AAATGTGGGGCACTCTTTCCATTTCTTACCCCAGTTTTTGAAGCCCTAAATATCGGTTTG
NM_022820.4         CTCTTAATATCACTCTTTCCATTTCTTACCCCAGTTTTTGAAGCCCTAAATATCGGTTTG
NM_001278921.1      AAATGTGGGGCACTCTTTCCATTTCTTACCCCAGTTTTTGAAGCCCTAAATATCGGTTTG
NM_057095.2         CTCTTAATATCACTCTTTCCATTTCTTACCCCAGTTTTTGAAGCCCTAAATATCGGTTTG
                    .:.* :. . **************************************************

HsCD00436736        TTTCCAAAAGATGTTACCCATTTTTTAAAAAATTCCATTGAAAGGATGAAAGAAAGTCGC
NM_022820.4         TTTCCAAAAGATGTTACCCATTTTTTAAAAAATTCCATTGAAAGGATGAAAGAAAGTCGC
NM_001278921.1      TTTCCAAAAGATGTTACCCATTTTTTAAAAAATTCCATTGAAAGGATGAAAGAAAGTCGC
NM_057095.2         TTTCCAAAAGATGTTACCCATTTTTTAAAAAATTCCATTGAAAGGATGAAAGAAAGTCGC
                    ************************************************************

HsCD00436736        CTCAAAGATAAACAAAAGCATCGAGTAGATTTCTTTCAACAGATGATCGACTCCCAGAAT
NM_022820.4         CTCAAAGATAAACAAAAGCATCGAGTAGATTTCTTTCAACAGATGATCGACTCCCAGAAT
NM_001278921.1      CTCAAAGATAAACAAAAGCATCGAGTAGATTTCTTTCAACAGATGATCGACTCCCAGAAT
NM_057095.2         CTCAAAGATAAACAAAAGCATCGAGTAGATTTCTTTCAACAGATGATCGACTCCCAGAAT
                    ************************************************************

HsCD00436736        TCCAAAGAAACAAAGTCCCATAAAGCTCTGTCTGATCTGGAGCTTGTGGCCCAGTCAATT
NM_022820.4         TCCAAAGAAACAAAGTCCCATAAAGCTCTGTCTGATCTGGAGCTTGTGGCCCAGTCAATT
NM_001278921.1      TCCAAAGAAACAAAGTCCCATAAAGCTCTGTCTGATCTGGAGCTTGTGGCCCAGTCAATT
NM_057095.2         TCCAAAGAAACAAAGTCCCATAAAGCTCTGTCTGATCTGGAGCTTGTGGCCCAGTCAATT
                    ************************************************************

HsCD00436736        ATCATCATTTTTGCTGCCTATGACACAACTAGCACCACTCTCCCCTTCATTATGTATGAA
NM_022820.4         ATCATCATTTTTGCTGCCTATGACACAACTAGCACCACTCTCCCCTTCATTATGTATGAA
NM_001278921.1      ATCATCATTTTTGCTGCCTATGACACAACTAGCACCACTCTCCCCTTCATTATGTATGAA
NM_057095.2         ATCATCATTTTTGCTGCCTATGACACAACTAGCACCACTCTCCCCTTCATTATGTATGAA
                    ************************************************************

HsCD00436736        CTGGCCACTCACCCTGATGTCCAGCAGAAACTGCAGGAGGAGATTGACGCAGTTTTACCC
NM_022820.4         CTGGCCACTCACCCTGATGTCCAGCAGAAACTGCAGGAGGAGATTGACGCAGTTTTACCC
NM_001278921.1      CTGGCCACTCACCCTGATGTCCAGCAGAAACTGCAGGAGGAGATTGACGCAGTTTTACCC
NM_057095.2         CTGGCCACTCACCCTGATGTCCAGCAGAAACTGCAGGAGGAGATTGACGCAGTTTTACCC
                    ************************************************************

HsCD00436736        AATAAGGCACCTGTCACCTACGATGCCCTGGTACAGATGGAGTACCTTGACATGGTGGTG
NM_022820.4         AATAAGGCACCTGTCACCTACGATGCCCTGGTACAGATGGAGTACCTTGACATGGTGGTG
NM_001278921.1      AATAAGGCACCTGTCACCTACGATGCCCTGGTACAGATGGAGTACCTTGACATGGTGGTG
NM_057095.2         AATAAGGCACCTGTCACCTACGATGCCCTGGTACAGATGGAGTACCTTGACATGGTGGTG
                    ************************************************************

HsCD00436736        AATGAAACGCTCAGATTATTCCCAGTTGTTAGTAGAGTTACGAGAGTCTGCAAGAAAGAT
NM_022820.4         AATGAAACGCTCAGATTATTCCCAGTTGTTAGTAGAGTTACGAGAGTCTGCAAGAAAGAT
NM_001278921.1      AATGAAACGCTCAGATTATTCCCAGTTGTTAGTAGAGTTACGAGAGTCTGCAAGAAAGAT
NM_057095.2         AATGAAACGCTCAGATTATTCCCAGTTGTTAGTAGAGTTACGAGAGTCTGCAAGAAAGAT
                    ************************************************************

HsCD00436736        ATTGAAATCAATGGAGTGTTCATTCCCAAAGGGTTAGCAGTGATGGTTCCAATCTATGCT
NM_022820.4         ATTGAAATCAATGGAGTGTTCATTCCCAAAGGGTTAGCAGTGATGGTTCCAATCTATGCT
NM_001278921.1      ATTGAAATCAATGGAGTGTTCATTCCCAAAGGGTTAGCAGTGATGGTTCCAATCTATGCT
NM_057095.2         ATTGAAATCAATGGAGTGTTCATTCCCAAAGGGTTAGCAGTGATGGTTCCAATCTATGCT
                    ************************************************************

HsCD00436736        CTTCACCATGACCCAAAGTACTGGACAGAGCCTGAGAAGTTCTGCCCTGAAAG---GTTC
NM_022820.4         CTTCACCATGACCCAAAGTACTGGACAGAGCCTGAGAAGTTCTGCCCTGAAAGTAGGTTC
NM_001278921.1      CTTCACCATGACCCAAAGTACTGGACAGAGCCTGAGAAGTTCTGCCCTGAAAG---GTTC
NM_057095.2         CTTCACCATGACCCAAAGTACTGGACAGAGCCTGAGAAGTTCTGCCCTGAAAG---GTTC
                    *****************************************************   ****

HsCD00436736        AGTAAGAAGAACAAGGACAGCATAGATCTTTACAGATACATACCTTTTGGAGCTGGACCC
NM_022820.4         AGTAAGAAGAACAAGGACAGCATAGATCTTTACAGATACATACCTTTTGGAGCTGGACCC
NM_001278921.1      AGTAAGAAGAACAAGGACAGCATAGATCTTTACAGATACATACCTTTTGGAGCTGGACCC
NM_057095.2         AGTAAGAAGAACAAGGACAGCATAGATCTTTACAGATACATACCTTTTGGAGCTGGACCC
                    ************************************************************

HsCD00436736        CGAAACTGCATTGGCATGAGGTTTGCTCTCACAAACATAAAACTTGCTGTCATTAGAGCA
NM_022820.4         CGAAACTGCATTGGCATGAGGTTTGCTCTCACAAACATAAAACTTGCTGTCATTAGAGCA
NM_001278921.1      CGAAACTGCATTGGCATGAGGTTTGCTCTCACAAACATAAAACTTGCTGTCATTAGAGCA
NM_057095.2         CGAAACTGCATTGGCATGAGGTTTGCTCTCACAAACATAAAACTTGCTGTCATTAGAGCA
                    ************************************************************

HsCD00436736        CTGCAGAACTTCTCCTTCAAACCTTGTAAAGAGACTCAGATCCCACTGAAATTAGACAAT
NM_022820.4         CTGCAGAACTTCTCCTTCAAACCTTGTAAAGAGACTCAGATCCCACTGAAATTAGACAAT
NM_001278921.1      CTGCAGAACTTCTCCTTCAAACCTTGTAAAGAGACTCAGATCCCACTGAAATTAGACAAT
NM_057095.2         CTGCAGAACTTCTCCTTCAAACCTTGTAAAGAGACTCAGATCCCACTGAAATTAGACAAT
                    ************************************************************

HsCD00436736        CTACCAATTCTTCAACCAGAAAAACCTATTGTTCTAAAAGTGCACTTAAGAGATGGGATT
NM_022820.4         CTACCAATTCTTCAACCAGAAAAACCTATTGTTCTAAAAGTGCACTTAAGAGATGGGATT
NM_001278921.1      CTACCAATTCTTCAACCAGAAAAACCTATTGTTCTAAAAGTGCACTTAAGAGATGGGATT
NM_057095.2         CTACCAATTCTTCAACCAGAAAAACCTATTGTTCTAAAAGTGCACTTAAGAGATGGGATT
                    ************************************************************

HsCD00436736        ACAAGTGGACCC---
NM_022820.4         ACAAGTGGACCCTGA
NM_001278921.1      ACAAGTGGACCCTGA
NM_057095.2         ACAAGTGGACCCTGA
                    ************   

Pathways

Reactome : Biological oxidations
Reactome : Cytochrome P450 - arranged by substrate type
Reactome : Metabolism
Reactome : Miscellaneous substrates
Reactome : Phase 1 - Functionalization of compounds
Reactome : Xenobiotics
WikiPathway : cytochrome P450
WikiPathway : metapathway biotransformation

Protein

HPRD : 05942

Clone

Original Clone ID : ccsbBroad304_12497

Organism


TAX_ID : 9606
Species Specific ID: 64816

Genome

Chromosome : 7
Map Location : 7q21.1
Ensembl : ENSG00000021461

Reagents

Labome : CYP3A43-antibody