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LGALS9 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  LGALS9
Gene Name:  lectin, galactoside-binding, soluble, 9
Sequence             
Original Clone ID: ccsbBroad304_00940
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00436780

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 970nts         Open reading frame : 1 to 970
Reference Sequence Alignment

ATGGCCTTCAGCGGTTCCCAGGCTCCCTACCTGAGTCCAGCTGTCCCCTTTTCTGGGACT
ATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCC
AGTGGAACCAGGTTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTC
CACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGCAGAACGGA
AGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGAC
CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGATCCTCTTCGTG
CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
CAGCTGTCCTACATCAGCTTCCAGCCTCCCGGCGTGTGGCCTGCCAACCCGGCTCCCATT
ACCCAGACAGTCATCCACACAGTGCAGAGCGCCCCTGGACAGATGTTCTCTACTCCCGCC
ATCCCACCTATGATGTACCCCCACCCCGCCTATCCGATGCCTTTCATCACCACCATTCTG
GGAGGGCTGTACCCATCCAAGTCCATCCTCCTGTCAGGCACTGTCCTGCCCAGTGCTCAG
AGGTTCCACATCAACCTGTGCTCTGGGAACCACATCGCCTTCCACCTGAACCCCCGTTTT
GATGAGAATGCTGTGGTCCGCAACACCCAGATCGACAACTCCTGGGGGTCTGAGGAGCGA
AGTCTGCCCCGAAAAATGCCCTTCGTCCGTGGCCAGAGCTTCTCAGTGTGGATCTTGTGT
GAAGCTCACTGCCTCAAGGTGGCCGTGGATGGTCAGCACCTGTTTGAATACTACCATCGC
CTGAGGAACCTGCCCACCATCAACAGACTGGAAGTGGGGGGCGACATCCAGCTGACCCAT
GTGCAGACA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LGALS9
Symbol Nomenclature : LGALS9
SYNONYM : HUAT; LGALS9A
Designation : ecalectin|gal-9|galectin 9|galectin-9|tumor antigen HOM-HD-21|urate transporter/channel protein
Full Nomenclature : lectin, galactoside-binding, soluble, 9
GENEID : 3965
HGNC : 6570
MIM : 601879
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00436780        ATGGCCTTCAGCGGTTCCCAGGCTCCCTACCTGAGTCCAGCTGTCCCCTTTTCTGGGACT
NM_009587.2         ATGGCCTTCAGCGGTTCCCAGGCTCCCTACCTGAGTCCAGCTGTCCCCTTTTCTGGGACT
NM_002308.3         ATGGCCTTCAGCGGTTCCCAGGCTCCCTACCTGAGTCCAGCTGTCCCCTTTTCTGGGACT
XM_005256770.1      ATGGCCTTCAGCGGTTGCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
                    **************** ************ ***** ***** ******************

HsCD00436780        ATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCC
NM_009587.2         ATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCC
NM_002308.3         ATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCC
XM_005256770.1      ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTGC
                    ** *****.*************** ********************.************ *

HsCD00436780        AGTGGAACCAGGTTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTC
NM_009587.2         AGTGGAACCAGGTTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTC
NM_002308.3         AGTGGAACCAGGTTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTC
XM_005256770.1      AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
                    *********************.********** ************** ************

HsCD00436780        CACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGCAGAACGGA
NM_009587.2         CACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGCAGAACGGA
NM_002308.3         CACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGCAGAACGGA
XM_005256770.1      CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
                    *********************** ******** ***********************.***

HsCD00436780        AGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGAC
NM_009587.2         AGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGAC
NM_002308.3         AGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGAC
XM_005256770.1      ACATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
                    * .********************** .******** ************************

HsCD00436780        CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGATCCTCTTCGTG
NM_009587.2         CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGATCCTCTTCGTG
NM_002308.3         CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGATCCTCTTCGTG
XM_005256770.1      CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
                    ************************************************* **********

HsCD00436780        CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
NM_009587.2         CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
NM_002308.3         CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
XM_005256770.1      CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
                    ************************************************************

HsCD00436780        CAGCTGTCCTACATCAGCTTCCAGCCTCCCG-----------------------------
NM_009587.2         CAGCTGTCCTACATCAGCTTCCAGAACCCCCGCACAGTCCCTGTTCAGCCTGCCTTCTCC
NM_002308.3         CAGCTGTCCTACATCAGCTTCCAGCCTCCCG-----------------------------
XM_005256770.1      CAGCTGTCCTACATCAGCTTCCAGCCTCCCAG----------------------------
                    ************************.. ***                              

HsCD00436780        ------------------------------------------------------------
NM_009587.2         ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
NM_002308.3         ------------------------------------------------------------
XM_005256770.1      ------------------------------------------------------------
                                                                                

HsCD00436780        -------GCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGTG
NM_009587.2         CCTCCCGGCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGTG
NM_002308.3         -------GCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGTG
XM_005256770.1      --------CGTGCGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
                            **** *************.*****************************.***

HsCD00436780        CAGAGCGCCCCTGGACAGATGTTCTCTACTCCCGCCATCCCACCTATGATGTACCCCCAC
NM_009587.2         CAGAGCGCCCCTGGACAGATGTTCTCTACTCCCGCCATCCCACCTATGATGTACCCCCAC
NM_002308.3         CAGAGCGCCCCTGGACAGATGTTCTCTACTCCCGCCATCCCACCTATGATGTACCCCCAC
XM_005256770.1      CAGAGTGCCTCTGGACAGATGTTCTCTACTCCCGCCATCCCACCTATGATGTACCCCCAC
                    ***** *** **************************************************

HsCD00436780        CCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAGTCC
NM_009587.2         CCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAGTCC
NM_002308.3         CCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAGTCC
XM_005256770.1      CCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAGTCC
                    ** ******************************* *************************

HsCD00436780        ATCCTCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGCTCT
NM_009587.2         ATCCTCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGCTCT
NM_002308.3         ATCCTCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGCTCT
XM_005256770.1      ATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGCTCT
                    ***.********************************************************

HsCD00436780        GGGAACCACATCGCCTTCCACCTGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGCAAC
NM_009587.2         GGGAACCACATCGCCTTCCACCTGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGCAAC
NM_002308.3         GGGAACCACATCGCCTTCCACCTGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGCAAC
XM_005256770.1      GGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGTAAC
                    ****.****************.********************************** ***

HsCD00436780        ACCCAGATCGACAACTCCTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCCTTC
NM_009587.2         ACCCAGATCGACAACTCCTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCCTTC
NM_002308.3         ACCCAGATCGACAACTCCTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCCTTC
XM_005256770.1      ACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCCTTC
                    *********.******* ******************************************

HsCD00436780        GTCCGTGGCCAGAGCTTCTCAGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTGGCC
NM_009587.2         GTCCGTGGCCAGAGCTTCTCAGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTGGCC
NM_002308.3         GTCCGTGGCCAGAGCTTCTCAGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTGGCC
XM_005256770.1      GTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTGGCC
                    *****:**************.***************************************

HsCD00436780        GTGGATGGTCAGCACCTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATCAAC
NM_009587.2         GTGGATGGTCAGCACCTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATCAAC
NM_002308.3         GTGGATGGTCAGCACCTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATCAAC
XM_005256770.1      GTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATCAAC
                    *************** ********************************************

HsCD00436780        AGACTGGAAGTGGGGGGCGACATCCAGCTGACCCATGTGCAGACA---
NM_009587.2         AGACTGGAAGTGGGGGGCGACATCCAGCTGACCCATGTGCAGACATAG
NM_002308.3         AGACTGGAAGTGGGGGGCGACATCCAGCTGACCCATGTGCAGACATAG
XM_005256770.1      AAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACATAG
                    *.************ ******************** *********   

Protein

SMART domain : Gal-bind_lectin : Galactoside-binding lectin
SMART domain : GLECT : Galectin
HPRD : 03528

Clone

Original Clone ID : ccsbBroad304_00940

Organism


TAX_ID : 9606
Species Specific ID: 3965

Genome

Chromosome : 17
Map Location : 17q11.2

Reagents

Labome : galectin-9-antibody