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FGF14 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  FGF14
Gene Name:  fibroblast growth factor 14
Sequence             
Original Clone ID: ccsbBroad304_00560
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00436828

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 757nts         Open reading frame : 1 to 757
Reference Sequence Alignment

ATGGTAAAACCGGTGCCCCTCTTCAGGAGAACTGATTTCAAATTATTATTATGCAACCAC
AAGGATCTCTTCTTTCTCAGGGTGTCTAAGCTGCTGGATTGCTTTTCGCCCAAATCAATG
TGGTTTCTTTGGAACATTTTCAGCAAAGGAACGCATATGCTGCAGTGTCTTTGTGGCAAG
AGTCTTAAGAAAAACAAGAACCCAACTGATCCCCAGCTCAAGGGTATAGTGACCAGGTTA
TATTGCAGGCAAGGCTACTACTTGCAAATGCACCCCGATGGAGCTCTCGATGGAACCAAG
GATGACAGCACTAATTCTACACTCTTCAACCTCATACCAGTGGGACTACGTGTTGTTGCC
ATCCAGGGAGTGAAAACAGGGTTGTATATAGCCATGAATGGAGAAGGTTACCTCTACCCA
TCAGAACTTTTTACCCCTGAATGCAAGTTTAAAGAATCTGTTTTTGAAAATTATTATGTA
ATCTACTCATCCATGTTGTACAGACAACAGGAATCTGGTAGAGCCTGGTTTTTGGGATTA
AATAAGGAAGGGCAAGCTATGAAAGGGAACAGAGTAAAGAAAACCAAACCAGCAGCTCAT
TTTCTACCCAAGCCATTGGAAGTTGCCATGTACCGAGAACCATCTTTGCATGATGTTGGG
GAAACGGTCCCGAAGCCTGGGGTGACGCCAAGTAAAAGCACAAGTGCGTCTGCAATAATG
AATGGAGGCAAACCAGTCAACAAGAGTAAGACAACA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FGF14
Symbol Nomenclature : FGF14
SYNONYM : FGF-14; FHF-4; FHF4; SCA27
Designation : bA397O8.2|fibroblast growth factor homologous factor 4
Full Nomenclature : fibroblast growth factor 14
GENEID : 2259
Locus Tag : RP11-397O8.6
HGNC : 3671
MIM : 601515
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00436828      ATGGTAAAACCGGTGCCCCTCTTCAGGAGAACTGATTTCAAATTATTATTATGCAACCAC
NM_175929.2       ATGGTAAAACCGGTGCCCCTCTTCAGGAGAACTGATTTCAAATTATTATTATGCAACCAC
NM_004115.3       ------------ATGGCCGCGGCCATCGCTAGCGGCTTGATCCGCCAGAAGCGGCAGGCG
                              .** **     **  . :*  *. ** *:.  . :.::. * .*  . 

HsCD00436828      AAGGATCTCTTCTTTCTCAGGGTGTCTAAGCTGCTGGATTGCTTTTCGCCCAAATCAATG
NM_175929.2       AAGGATCTCTTCTTTCTCAGGGTGTCTAAGCTGCTGGATTGCTTTTCGCCCAAATCAATG
NM_004115.3       CGGGAGCAGCACTGGGACCGGCCGTCTGCCAGCAGGAGGCGGAGCAGCCCCAGCAAGAAC
                  ..*** *:  :**   :*.**  ****.. .  . *..  * :  :  ****..:..*: 

HsCD00436828      TGGTTTCTTTGGAACATTTTCAGCAAAGGAACGCATATGCTGCAGTGTCTTTGTGGCAAG
NM_175929.2       TGGTTTCTTTGGAACATTTTCAGCAAAGGAACGCATATGCTGCAGTGTCTTTGTGGCAAG
NM_004115.3       CGCGGGCTCTGCAACGGCAACCTGGTGGATATCTTCTCCAAAGTGCGCATCTTCGGCCTC
                   *    ** ** ***.  ::*.  .:.*.:*   : :  .:. :* * .* *  ***.: 

HsCD00436828      AGTCTTAAGAAAAACAAGAACCCAACTGATCCCCAGCTCAAGGGTATAGTGACCAGGTTA
NM_175929.2       AGTCTTAAGAAAAACAAGAACCCAACTGATCCCCAGCTCAAGGGTATAGTGACCAGGTTA
NM_004115.3       AAGAAGCG---CAGGTTGCGGCGCCAAGATCCCCAGCTCAAGGGTATAGTGACCAGGTTA
                  *. .: ..   .*. ::*.. * ...:*********************************

HsCD00436828      TATTGCAGGCAAGGCTACTACTTGCAAATGCACCCCGATGGAGCTCTCGATGGAACCAAG
NM_175929.2       TATTGCAGGCAAGGCTACTACTTGCAAATGCACCCCGATGGAGCTCTCGATGGAACCAAG
NM_004115.3       TATTGCAGGCAAGGCTACTACTTGCAAATGCACCCCGATGGAGCTCTCGATGGAACCAAG
                  ************************************************************

HsCD00436828      GATGACAGCACTAATTCTACACTCTTCAACCTCATACCAGTGGGACTACGTGTTGTTGCC
NM_175929.2       GATGACAGCACTAATTCTACACTCTTCAACCTCATACCAGTGGGACTACGTGTTGTTGCC
NM_004115.3       GATGACAGCACTAATTCTACACTCTTCAACCTCATACCAGTGGGACTACGTGTTGTTGCC
                  ************************************************************

HsCD00436828      ATCCAGGGAGTGAAAACAGGGTTGTATATAGCCATGAATGGAGAAGGTTACCTCTACCCA
NM_175929.2       ATCCAGGGAGTGAAAACAGGGTTGTATATAGCCATGAATGGAGAAGGTTACCTCTACCCA
NM_004115.3       ATCCAGGGAGTGAAAACAGGGTTGTATATAGCCATGAATGGAGAAGGTTACCTCTACCCA
                  ************************************************************

HsCD00436828      TCAGAACTTTTTACCCCTGAATGCAAGTTTAAAGAATCTGTTTTTGAAAATTATTATGTA
NM_175929.2       TCAGAACTTTTTACCCCTGAATGCAAGTTTAAAGAATCTGTTTTTGAAAATTATTATGTA
NM_004115.3       TCAGAACTTTTTACCCCTGAATGCAAGTTTAAAGAATCTGTTTTTGAAAATTATTATGTA
                  ************************************************************

HsCD00436828      ATCTACTCATCCATGTTGTACAGACAACAGGAATCTGGTAGAGCCTGGTTTTTGGGATTA
NM_175929.2       ATCTACTCATCCATGTTGTACAGACAACAGGAATCTGGTAGAGCCTGGTTTTTGGGATTA
NM_004115.3       ATCTACTCATCCATGTTGTACAGACAACAGGAATCTGGTAGAGCCTGGTTTTTGGGATTA
                  ************************************************************

HsCD00436828      AATAAGGAAGGGCAAGCTATGAAAGGGAACAGAGTAAAGAAAACCAAACCAGCAGCTCAT
NM_175929.2       AATAAGGAAGGGCAAGCTATGAAAGGGAACAGAGTAAAGAAAACCAAACCAGCAGCTCAT
NM_004115.3       AATAAGGAAGGGCAAGCTATGAAAGGGAACAGAGTAAAGAAAACCAAACCAGCAGCTCAT
                  ************************************************************

HsCD00436828      TTTCTACCCAAGCCATTGGAAGTTGCCATGTACCGAGAACCATCTTTGCATGATGTTGGG
NM_175929.2       TTTCTACCCAAGCCATTGGAAGTTGCCATGTACCGAGAACCATCTTTGCATGATGTTGGG
NM_004115.3       TTTCTACCCAAGCCATTGGAAGTTGCCATGTACCGAGAACCATCTTTGCATGATGTTGGG
                  ************************************************************

HsCD00436828      GAAACGGTCCCGAAGCCTGGGGTGACGCCAAGTAAAAGCACAAGTGCGTCTGCAATAATG
NM_175929.2       GAAACGGTCCCGAAGCCTGGGGTGACGCCAAGTAAAAGCACAAGTGCGTCTGCAATAATG
NM_004115.3       GAAACGGTCCCGAAGCCTGGGGTGACGCCAAGTAAAAGCACAAGTGCGTCTGCAATAATG
                  ************************************************************

HsCD00436828      AATGGAGGCAAACCAGTCAACAAGAGTAAGACAACA---
NM_175929.2       AATGGAGGCAAACCAGTCAACAAGAGTAAGACAACATAG
NM_004115.3       AATGGAGGCAAACCAGTCAACAAGAGTAAGACAACATAG
                  ************************************   

Pathways

Panther : FGF signaling pathway
WikiPathway : Regulation of Actin Cytoskeleton

Clone

Original Clone ID : ccsbBroad304_00560

Organism


TAX_ID : 9606
Species Specific ID: 2259

Genome

Chromosome : 13
Map Location : 13q34

Reagents

Labome : FGF14-antibody