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TBRG1 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TBRG1
Gene Name:  transforming growth factor beta regulator 1
Sequence             
Original Clone ID: ccsbBroad304_12875
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00436915

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 562nts         Open reading frame : 1 to 562
Reference Sequence Alignment

ATGAAGTGCCCAGACCAGAAGTGTCTATATACCTGTCAGATCAAGGATGGTGGTGTGCAG
CCTCAGTTTGAAATTGTTCCTGAAGATGACCCCCAGAATGCCATTGTCAGCTCTTCTGCA
GATGCTTGTCATGCAGAACTGCTCAGGACTATAAGCACTACTATGGGGAAACTAATGCCT
AACCTGCTTCCAGCTGGAGCTGACTTTTTTGGATTTTCTCATCCAGCCATCCACAACCTG
ATCCAGAGCTGTCCAGGAGCTCGAAAATGCATCAATTACCAGTGGGTGAAATTTGATGTG
TGCAAACCTGGAGATGGGCAGCTACCTGAGGGGCTGCCGGAGAATGATGCAGCTATGAGC
TTTGAAGCCTTTCAGAGACAGATCTTTGATGAAGATCAGAATGATCCCCTTCTGCCAGGA
TCCTTGGACCTCCCAGAGCTTCAGCCTGCAGCCTTTGTGTCTTCTTACCAGCCCATGTAC
CTGACACATGAACCCTTGGTAGATACTCACCTGCAGCACTTGAAGTCTCCATCACAGGGT
AGCCCAATTCAGTCTTCAGAT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  None
Description: with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: EvNO00877063
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TBRG1
Symbol Nomenclature : TBRG1
SYNONYM : NIAM; TB-5
Designation : nuclear interactor of ARF and MDM2
Full Nomenclature : transforming growth factor beta regulator 1
GENEID : 84897
HGNC : 29551
MIM : 610614
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00436915        ------------------------------------------------------------
XM_005271709.1      ATGAGCCTGCTGGACGGCCTCGCTTCCTCGCCGCGGGCTCCGCTGCAGTCCAGCAAGGCC
NM_032811.2         ATGAGCCTGCTGGACGGCCTCGCTTCCTCGCCGCGGGCTCCGCTGCAGTCCAGCAAGGCC
                                                                                

HsCD00436915        ------------------------------------------------------------
XM_005271709.1      AGGATGAAAAAGCTCCCGAAGAAGAGCCAGAATGAGAAGTACCGGCTGAAGTACCTGCGG
NM_032811.2         AGGATGAAAAAGCTCCCGAAGAAGAGCCAGAATGAGAAGTACCGGCTGAAGTACCTGCGG
                                                                                

HsCD00436915        ------------------------------------------------------------
XM_005271709.1      CTGCGCAAAGCGGCCAAGGCCACG------------------------------------
NM_032811.2         CTGCGCAAAGCGGCCAAGGCCACGGTGTTTGAAAATGCTGCTATTTGTGATGAAATTGCT
                                                                                

HsCD00436915        ------------------------------------------------------------
XM_005271709.1      ------------------------------------------------------------
NM_032811.2         CGTCTTGAGGAAAAATTTCTTAAAGCAAAAGAAGAAAGAAGGTACTTGCTAAAGAAGCTC
                                                                                

HsCD00436915        ------------------------------------------------------------
XM_005271709.1      ------------------------------------------------------------
NM_032811.2         CTCCAGCTTCAGGCTCTAACTGAAGGGGAAGTACAGGCTGCAGCTCCTTCCCACAGTTCC
                                                                                

HsCD00436915        ------------------------------------------------------------
XM_005271709.1      ------------------------------------------------------------
NM_032811.2         AGTTTGCCCCTGACTTATGGTGTGGCCAGCTCTGTGGGAACTATACAGGGAGCTGGGCCT
                                                                                

HsCD00436915        ------------------------------------------------------------
XM_005271709.1      ------------------------------------------------------------
NM_032811.2         ATTTCAGGGCCCAGCACTGGGGCTGAGGAACCATTTGGGAAGAAAACTAAGAAGGAGAAA
                                                                                

HsCD00436915        ------------------------------------------------------------
XM_005271709.1      ------------------------------------------------------------
NM_032811.2         AAAGAAAAAGGCAAAGAGAACAACAAACTGGAAGTTCTGAAGAAAACATGCAAGAAAAAG
                                                                                

HsCD00436915        ------------------------------------------------------------
XM_005271709.1      ------------------------------------------------------------
NM_032811.2         AAAATGGCGGGAGGTGCTCGCAAGCTGGTTCAGCCCATTGCCCTGGATCCCTCAGGACGG
                                                                                

HsCD00436915        ------------------------------------------------------------
XM_005271709.1      ---------------------------------------------GTGTTTATCATCACC
NM_032811.2         CCTGTGTTCCCCATCGGACTAGGGGGTCTAACAGTATATAGCCTGGGGGAGATCATCACC
                                                                                

HsCD00436915        ------------------------------------------------------------
XM_005271709.1      GACCGACCTGGCTTTCATGATGAGAGTGCCATCTACCCCGTGGGCTATTGCAGTACTCGA
NM_032811.2         GACCGACCTGGCTTTCATGATGAGAGTGCCATCTACCCCGTGGGCTATTGCAGTACTCGA
                                                                                

HsCD00436915        ------------ATGAAGTGCCCAGACCAGAAGTGTCTATATACCTGTCAGATCAAGGAT
XM_005271709.1      ATATATGCCAGCATGAAGTGCCCAGACCAGAAGTGTCTATATACCTGTCAGATCAAGGAT
NM_032811.2         ATATATGCCAGCATGAAGTGCCCAGACCAGAAGTGTCTATATACCTGTCAGATCAAGGAT
                                ************************************************

HsCD00436915        GGTGGTGTGCAGCCTCAGTTTGAAATTGTTCCTGAAGATGACCCCCAGAATGCCATTGTC
XM_005271709.1      GGTGGTGTGCAGCCTCAGTTTGAAATTGTTCCTGAAGATGACCCCCAGAATGCCATTGTC
NM_032811.2         GGTGGTGTGCAGCCTCAGTTTGAAATTGTTCCTGAAGATGACCCCCAGAATGCCATTGTC
                    ************************************************************

HsCD00436915        AGCTCTTCTGCAGATGCTTGTCATGCAGAACTGCTCAGGACTATAAGCACTACTATGGGG
XM_005271709.1      AGCTCTTCTGCAGATGCTTGTCATGCAGAACTGCTCAGGACTATAAGCACTACTATGGGG
NM_032811.2         AGCTCTTCTGCAGATGCTTGTCATGCAGAACTGCTCAGGACTATAAGCACTACTATGGGG
                    ************************************************************

HsCD00436915        AAACTAATGCCTAACCTGCTTCCAGCTGGAGCTGACTTTTTTGGATTTTCTCATCCAGCC
XM_005271709.1      AAACTAATGCCTAACCTGCTTCCAGCTGGAGCTGACTTTTTTGGATTTTCTCATCCAGCC
NM_032811.2         AAACTAATGCCTAACCTGCTTCCAGCTGGAGCTGACTTTTTTGGATTTTCTCATCCAGCC
                    ************************************************************

HsCD00436915        ATCCACAACCTGATCCAGAGCTGTCCAGGAGCTCGAAAATGCATCAATTACCAGTGGGTG
XM_005271709.1      ATCCACAACCTGATCCAGAGCTGTCCAGGAGCTCGAAAATGCATCAATTACCAGTGGGTG
NM_032811.2         ATCCACAACCTGATCCAGAGCTGTCCAGGAGCTCGAAAATGCATCAATTACCAGTGGGTG
                    ************************************************************

HsCD00436915        AAATTTGATGTGTGCAAACCTGGAGATGGGCAGCTACCTGAGGGGCTGCCGGAGAATGAT
XM_005271709.1      AAATTTGATGTGTGCAAACCTGGAGATGGGCAGCTACCTGAGGGGCTGCCGGAGAATGAT
NM_032811.2         AAATTTGATGTGTGCAAACCTGGAGATGGGCAGCTACCTGAGGGGCTGCCGGAGAATGAT
                    ************************************************************

HsCD00436915        GCAGCTATGAGCTTTGAAGCCTTTCAGAGACAGATCTTTGATGAAGATCAGAATGATCCC
XM_005271709.1      GCAGCTATGAGCTTTGAAGCCTTTCAGAGACAGATCTTTGATGAAGATCAGAATGATCCC
NM_032811.2         GCAGCTATGAGCTTTGAAGCCTTTCAGAGACAGATCTTTGATGAAGATCAGAATGATCCC
                    ************************************************************

HsCD00436915        CTTCTGCCAGGATCCTTGGACCTCCCAGAGCTTCAGCCTGCAGCCTTTGTGTCTTCTTAC
XM_005271709.1      CTTCTGCCAGGATCCTTGGACCTCCCAGAGCTTCAGCCTGCAGCCTTTGTGTCTTCTTAC
NM_032811.2         CTTCTGCCAGGATCCTTGGACCTCCCAGAGCTTCAGCCTGCAGCCTTTGTGTCTTCTTAC
                    ************************************************************

HsCD00436915        CAGCCCATGTACCTGACACATGAACCCTTGGTAGATACTCACCTGCAGCACTTGAAGTCT
XM_005271709.1      CAGCCCATGTACCTGACACATGAACCCTTGGTAGATACTCACCTGCAGCACTTGAAGTCT
NM_032811.2         CAGCCCATGTACCTGACACATGAACCCTTGGTAGATACTCACCTGCAGCACTTGAAGTCT
                    ************************************************************

HsCD00436915        CCATCACAGGGTAGCCCAATTCAGTCTTCAGAT---
XM_005271709.1      CCATCACAGGGTAGCCCAATTCAGTCTTCAGATTGA
NM_032811.2         CCATCACAGGGTAGCCCAATTCAGTCTTCAGATTGA
                    *********************************   

Protein

SMART domain : FYRC : FY-rich domain, C-terminal region
HPRD : 15477

Clone

Original Clone ID : ccsbBroad304_12875

Organism


TAX_ID : 9606
Species Specific ID: 84897

Genome

Chromosome : 11
Map Location : 11q24.2

Reagents

Labome : TBRG1-antibody